PMC:7795856 / 38577-39514 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7795856","sourcedb":"PMC","sourceid":"7795856","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7795856","text":"Figure 2 Analysis of Tα1-PAS N-acetylation, peptide purity, and hydrodynamic volume. (a) The deconvoluted ESI-MS spectrum of the Tα1-PAS flow-through fraction after the subtractive AEX step (see text) shows a molecular mass of 52,734.56 Da, corresponding to N-acetylated Tα1-PAS (calc. mass: 52,734.56 Da). (b) ESI-MS analysis of the bound and eluted peptide fraction from the same chromatography reveals a molecular mass of 52,692.37 Da, which matches the calculated mass of non-acetylated Tα1-PAS (52,692.54 Da). (c) Reverse-phase chromatography of the flow-through fraction indicates a purity \u003e 96% for N-acetylated Tα1-PAS. (d) Analytical SEC of the finally purified Tα1-PAS in the presence of phosphate-buffered saline (PBS) results in a single peak with an elution volume of 13.5 mL. Comparison with a half-logarithmic calibration curve (inset) indicates an expanded hydrodynamic volume with an apparent molecular size of 557 kDa.","divisions":[{"label":"label","span":{"begin":0,"end":8}}],"tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"826","span":{"begin":22,"end":25},"obj":"Gene"},{"id":"827","span":{"begin":130,"end":133},"obj":"Gene"},{"id":"828","span":{"begin":672,"end":675},"obj":"Gene"},{"id":"829","span":{"begin":261,"end":271},"obj":"Chemical"},{"id":"830","span":{"begin":607,"end":627},"obj":"Chemical"},{"id":"831","span":{"begin":699,"end":724},"obj":"Chemical"}],"attributes":[{"id":"A826","pred":"tao:has_database_id","subj":"826","obj":"Gene:134864"},{"id":"A827","pred":"tao:has_database_id","subj":"827","obj":"Gene:134864"},{"id":"A828","pred":"tao:has_database_id","subj":"828","obj":"Gene:134864"},{"subj":"826","pred":"source","obj":"LitCovid-PubTator"},{"subj":"827","pred":"source","obj":"LitCovid-PubTator"},{"subj":"828","pred":"source","obj":"LitCovid-PubTator"},{"subj":"829","pred":"source","obj":"LitCovid-PubTator"},{"subj":"830","pred":"source","obj":"LitCovid-PubTator"},{"subj":"831","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T297","span":{"begin":0,"end":292},"obj":"Sentence"},{"id":"T298","span":{"begin":293,"end":790},"obj":"Sentence"},{"id":"T299","span":{"begin":791,"end":937},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T297","pred":"source","obj":"LitCovid-sentences"},{"subj":"T298","pred":"source","obj":"LitCovid-sentences"},{"subj":"T299","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#ec93c9","default":true},{"id":"LitCovid-sentences","color":"#93e3ec"}]}]}}