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PMC:7795856 / 38577-39514 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
826 22-25 Gene denotes Tα1 Gene:134864
827 130-133 Gene denotes Tα1 Gene:134864
828 672-675 Gene denotes Tα1 Gene:134864
829 261-271 Chemical denotes acetylated
830 607-627 Chemical denotes N-acetylated Tα1-PAS
831 699-724 Chemical denotes phosphate-buffered saline

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T297 0-292 Sentence denotes Figure 2 Analysis of Tα1-PAS N-acetylation, peptide purity, and hydrodynamic volume. (a) The deconvoluted ESI-MS spectrum of the Tα1-PAS flow-through fraction after the subtractive AEX step (see text) shows a molecular mass of 52,734.56 Da, corresponding to N-acetylated Tα1-PAS (calc. mass:
T298 293-790 Sentence denotes 52,734.56 Da). (b) ESI-MS analysis of the bound and eluted peptide fraction from the same chromatography reveals a molecular mass of 52,692.37 Da, which matches the calculated mass of non-acetylated Tα1-PAS (52,692.54 Da). (c) Reverse-phase chromatography of the flow-through fraction indicates a purity > 96% for N-acetylated Tα1-PAS. (d) Analytical SEC of the finally purified Tα1-PAS in the presence of phosphate-buffered saline (PBS) results in a single peak with an elution volume of 13.5 mL.
T299 791-937 Sentence denotes Comparison with a half-logarithmic calibration curve (inset) indicates an expanded hydrodynamic volume with an apparent molecular size of 557 kDa.