PMC:7795856 / 29079-29973 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"522","span":{"begin":615,"end":635},"obj":"Gene"},{"id":"523","span":{"begin":815,"end":835},"obj":"Gene"},{"id":"524","span":{"begin":637,"end":639},"obj":"Gene"},{"id":"525","span":{"begin":591,"end":596},"obj":"Species"},{"id":"526","span":{"begin":91,"end":99},"obj":"Chemical"},{"id":"527","span":{"begin":151,"end":187},"obj":"Chemical"},{"id":"528","span":{"begin":189,"end":193},"obj":"Chemical"},{"id":"529","span":{"begin":396,"end":404},"obj":"Chemical"},{"id":"530","span":{"begin":284,"end":303},"obj":"Disease"},{"id":"531","span":{"begin":533,"end":538},"obj":"Disease"}],"attributes":[{"id":"A522","pred":"tao:has_database_id","subj":"522","obj":"Gene:16870"},{"id":"A523","pred":"tao:has_database_id","subj":"523","obj":"Gene:16870"},{"id":"A524","pred":"tao:has_database_id","subj":"524","obj":"Gene:16870"},{"id":"A525","pred":"tao:has_database_id","subj":"525","obj":"Tax:10090"},{"id":"A526","pred":"tao:has_database_id","subj":"526","obj":"MESH:C026272"},{"id":"A527","pred":"tao:has_database_id","subj":"527","obj":"MESH:C008550"},{"id":"A528","pred":"tao:has_database_id","subj":"528","obj":"MESH:C008550"},{"id":"A529","pred":"tao:has_database_id","subj":"529","obj":"MESH:D011136"},{"id":"A530","pred":"tao:has_database_id","subj":"530","obj":"MESH:D034721"},{"id":"A531","pred":"tao:has_database_id","subj":"531","obj":"MESH:D011535"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"4.6. Western Blot Analysis\nThe whole cell protein extract was applied to a 4–12% SurePAGE™ Bis–Tris gradient gel (Genscript, Piscataway, NJ, USA) with 3-(N-morpholino)propanesulfonic acid (MOPS) as running buffer and electrotransferred onto a nitrocellulose membrane using an iBlot 1 dry blotting system (ThermoFisher, Waltham, MA, USA). After washing 3 times with PBS supplemented with 0.1% v/v Tween 20 (PBS/T), the membrane was incubated with 1 µg/mL anti-PAS antibody in PBS/T for 1 h at RT. The membrane was washed 3 times with PBS/T and incubated with a 1:5000 dilution of a goat anti-mouse IgG (Fc-specific) alkaline phosphatase (AP) conjugate (Merck) for 1 h. After washing twice with PBS/T and twice with PBS, the blot was developed by a chromogenic reaction using BCIP (37.5 µg/mL) and NBT (150 µg/mL) in alkaline phosphatase buffer (100 mM Tris/HCl, pH 8.8, 100 mM NaCl, 5 mM MgCl2)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T170","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T171","span":{"begin":5,"end":26},"obj":"Sentence"},{"id":"T172","span":{"begin":27,"end":337},"obj":"Sentence"},{"id":"T173","span":{"begin":338,"end":495},"obj":"Sentence"},{"id":"T174","span":{"begin":496,"end":667},"obj":"Sentence"},{"id":"T175","span":{"begin":668,"end":894},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"4.6. Western Blot Analysis\nThe whole cell protein extract was applied to a 4–12% SurePAGE™ Bis–Tris gradient gel (Genscript, Piscataway, NJ, USA) with 3-(N-morpholino)propanesulfonic acid (MOPS) as running buffer and electrotransferred onto a nitrocellulose membrane using an iBlot 1 dry blotting system (ThermoFisher, Waltham, MA, USA). After washing 3 times with PBS supplemented with 0.1% v/v Tween 20 (PBS/T), the membrane was incubated with 1 µg/mL anti-PAS antibody in PBS/T for 1 h at RT. The membrane was washed 3 times with PBS/T and incubated with a 1:5000 dilution of a goat anti-mouse IgG (Fc-specific) alkaline phosphatase (AP) conjugate (Merck) for 1 h. After washing twice with PBS/T and twice with PBS, the blot was developed by a chromogenic reaction using BCIP (37.5 µg/mL) and NBT (150 µg/mL) in alkaline phosphatase buffer (100 mM Tris/HCl, pH 8.8, 100 mM NaCl, 5 mM MgCl2)."}