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PMC:7795856 / 29079-29973 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
522 615-635 Gene denotes alkaline phosphatase Gene:16870
523 815-835 Gene denotes alkaline phosphatase Gene:16870
524 637-639 Gene denotes AP Gene:16870
525 591-596 Species denotes mouse Tax:10090
526 91-99 Chemical denotes Bis–Tris MESH:C026272
527 151-187 Chemical denotes 3-(N-morpholino)propanesulfonic acid MESH:C008550
528 189-193 Chemical denotes MOPS MESH:C008550
529 396-404 Chemical denotes Tween 20 MESH:D011136
530 284-303 Disease denotes dry blotting system MESH:D034721
531 533-538 Disease denotes PBS/T MESH:D011535

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T170 0-4 Sentence denotes 4.6.
T171 5-26 Sentence denotes Western Blot Analysis
T172 27-337 Sentence denotes The whole cell protein extract was applied to a 4–12% SurePAGE™ Bis–Tris gradient gel (Genscript, Piscataway, NJ, USA) with 3-(N-morpholino)propanesulfonic acid (MOPS) as running buffer and electrotransferred onto a nitrocellulose membrane using an iBlot 1 dry blotting system (ThermoFisher, Waltham, MA, USA).
T173 338-495 Sentence denotes After washing 3 times with PBS supplemented with 0.1% v/v Tween 20 (PBS/T), the membrane was incubated with 1 µg/mL anti-PAS antibody in PBS/T for 1 h at RT.
T174 496-667 Sentence denotes The membrane was washed 3 times with PBS/T and incubated with a 1:5000 dilution of a goat anti-mouse IgG (Fc-specific) alkaline phosphatase (AP) conjugate (Merck) for 1 h.
T175 668-894 Sentence denotes After washing twice with PBS/T and twice with PBS, the blot was developed by a chromogenic reaction using BCIP (37.5 µg/mL) and NBT (150 µg/mL) in alkaline phosphatase buffer (100 mM Tris/HCl, pH 8.8, 100 mM NaCl, 5 mM MgCl2).