PMC:7795856 / 29079-29973 JSON TXT

Annnotations TAB JSON ListView MergeView

Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
522	615-635	Gene	denotes	alkaline phosphatase	Gene:16870
523	815-835	Gene	denotes	alkaline phosphatase	Gene:16870
524	637-639	Gene	denotes	AP	Gene:16870
525	591-596	Species	denotes	mouse	Tax:10090
526	91-99	Chemical	denotes	Bis–Tris	MESH:C026272
527	151-187	Chemical	denotes	3-(N-morpholino)propanesulfonic acid	MESH:C008550
528	189-193	Chemical	denotes	MOPS	MESH:C008550
529	396-404	Chemical	denotes	Tween 20	MESH:D011136
530	284-303	Disease	denotes	dry blotting system	MESH:D034721
531	533-538	Disease	denotes	PBS/T	MESH:D011535

Id	Subject	Object	Predicate	Lexical cue
T170	0-4	Sentence	denotes	4.6.
T171	5-26	Sentence	denotes	Western Blot Analysis
T172	27-337	Sentence	denotes	The whole cell protein extract was applied to a 4–12% SurePAGE™ Bis–Tris gradient gel (Genscript, Piscataway, NJ, USA) with 3-(N-morpholino)propanesulfonic acid (MOPS) as running buffer and electrotransferred onto a nitrocellulose membrane using an iBlot 1 dry blotting system (ThermoFisher, Waltham, MA, USA).
T173	338-495	Sentence	denotes	After washing 3 times with PBS supplemented with 0.1% v/v Tween 20 (PBS/T), the membrane was incubated with 1 µg/mL anti-PAS antibody in PBS/T for 1 h at RT.
T174	496-667	Sentence	denotes	The membrane was washed 3 times with PBS/T and incubated with a 1:5000 dilution of a goat anti-mouse IgG (Fc-specific) alkaline phosphatase (AP) conjugate (Merck) for 1 h.
T175	668-894	Sentence	denotes	After washing twice with PBS/T and twice with PBS, the blot was developed by a chromogenic reaction using BCIP (37.5 µg/mL) and NBT (150 µg/mL) in alkaline phosphatase buffer (100 mM Tris/HCl, pH 8.8, 100 mM NaCl, 5 mM MgCl2).