PMC:7605337 / 9414-10228 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7605337","sourcedb":"PMC","sourceid":"7605337","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7605337","text":"It is not known whether these mutations are linked to the severity of coronavirus in these regions. Starr and co-workers25 performed a deep mutational scanning of nCOV-2019 RBD and used flow cytometry to measure the effect of single mutations on the expression of the folded protein as well as its binding affinity to ACE2. They showed that RBD is very tolerant to these mutations to maintain its expression level as well as binding affinity to ACE2 in most cases. According to their results, most natural mutations exert similar binding affinities to ACE2 as wild-type nCOV-2019. Furthermore, they showed that mutations at critical positions at the RBD-ACE2 interface at nCOV-2019 such as residues Q493 and Q498 do not reduce the binding affinity to ACE2 which shows the substantial plasticity of the interface.25","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T36","span":{"begin":275,"end":282},"obj":"Body_part"}],"attributes":[{"id":"A36","pred":"fma_id","subj":"T36","obj":"http://purl.org/sig/ont/fma/fma67257"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T72","span":{"begin":163,"end":172},"obj":"Disease"},{"id":"T73","span":{"begin":570,"end":579},"obj":"Disease"},{"id":"T74","span":{"begin":672,"end":681},"obj":"Disease"}],"attributes":[{"id":"A72","pred":"mondo_id","subj":"T72","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A73","pred":"mondo_id","subj":"T73","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A74","pred":"mondo_id","subj":"T74","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T73","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T74","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T76","span":{"begin":133,"end":134},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"attributes":[{"subj":"T76","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T37","span":{"begin":275,"end":282},"obj":"Chemical"}],"attributes":[{"id":"A37","pred":"chebi_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"subj":"T37","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T47","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"T48","span":{"begin":100,"end":323},"obj":"Sentence"},{"id":"T49","span":{"begin":324,"end":464},"obj":"Sentence"},{"id":"T50","span":{"begin":465,"end":580},"obj":"Sentence"},{"id":"T51","span":{"begin":581,"end":814},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T47","pred":"source","obj":"LitCovid-sentences"},{"subj":"T48","pred":"source","obj":"LitCovid-sentences"},{"subj":"T49","pred":"source","obj":"LitCovid-sentences"},{"subj":"T50","pred":"source","obj":"LitCovid-sentences"},{"subj":"T51","pred":"source","obj":"LitCovid-sentences"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"265","span":{"begin":318,"end":322},"obj":"Gene"},{"id":"266","span":{"begin":445,"end":449},"obj":"Gene"},{"id":"267","span":{"begin":552,"end":556},"obj":"Gene"},{"id":"268","span":{"begin":654,"end":658},"obj":"Gene"},{"id":"269","span":{"begin":751,"end":755},"obj":"Gene"},{"id":"270","span":{"begin":70,"end":81},"obj":"Species"},{"id":"271","span":{"begin":163,"end":167},"obj":"Species"},{"id":"272","span":{"begin":570,"end":574},"obj":"Species"},{"id":"273","span":{"begin":672,"end":676},"obj":"Species"}],"attributes":[{"id":"A265","pred":"tao:has_database_id","subj":"265","obj":"Gene:59272"},{"id":"A266","pred":"tao:has_database_id","subj":"266","obj":"Gene:59272"},{"id":"A267","pred":"tao:has_database_id","subj":"267","obj":"Gene:59272"},{"id":"A268","pred":"tao:has_database_id","subj":"268","obj":"Gene:59272"},{"id":"A269","pred":"tao:has_database_id","subj":"269","obj":"Gene:59272"},{"id":"A270","pred":"tao:has_database_id","subj":"270","obj":"Tax:11118"},{"id":"A271","pred":"tao:has_database_id","subj":"271","obj":"Tax:2697049"},{"id":"A272","pred":"tao:has_database_id","subj":"272","obj":"Tax:2697049"},{"id":"A273","pred":"tao:has_database_id","subj":"273","obj":"Tax:2697049"},{"subj":"265","pred":"source","obj":"LitCovid-PubTator"},{"subj":"266","pred":"source","obj":"LitCovid-PubTator"},{"subj":"267","pred":"source","obj":"LitCovid-PubTator"},{"subj":"268","pred":"source","obj":"LitCovid-PubTator"},{"subj":"269","pred":"source","obj":"LitCovid-PubTator"},{"subj":"270","pred":"source","obj":"LitCovid-PubTator"},{"subj":"271","pred":"source","obj":"LitCovid-PubTator"},{"subj":"272","pred":"source","obj":"LitCovid-PubTator"},{"subj":"273","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#ec93a2","default":true},{"id":"LitCovid-PD-MONDO","color":"#93bcec"},{"id":"LitCovid-PD-CLO","color":"#d6ec93"},{"id":"LitCovid-PD-CHEBI","color":"#e893ec"},{"id":"LitCovid-sentences","color":"#93ecce"},{"id":"LitCovid-PubTator","color":"#ecb493"}]}]}}