PMC:7291971 / 40471-41123
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7291971","sourcedb":"PMC","sourceid":"7291971","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7291971","text":"4.1.7 General method C for the synthesis of compounds 1, 3, 5–7, 9, 10-16\nThe synthesis intermediate was treated at room temperature with TFA in water (8/2, conc. 0.14 M). After stirring for 3–6 h until completion of reaction (TLC DCM/MeOH), solvents were removed and the residue was coevaporated three times with 7 M NH3 in MeOH. The residue was purified by flash column chromatography on a reversed-phase silica gel column C18 (4 g, 40 μm) with a linear gradient of acetonitrile in TEAAc buffer 50 mM, pH 7. The fractions containing the pure compound were pooled, concentrated and lyophilized with water/dioxane to give desired compound as a 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