PMC:7143804 / 47008-47754 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"307","span":{"begin":381,"end":386},"obj":"Chemical"}],"attributes":[{"id":"A307","pred":"tao:has_database_id","subj":"307","obj":"MESH:D014867"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28]."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T326","span":{"begin":106,"end":107},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T327","span":{"begin":143,"end":144},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T328","span":{"begin":312,"end":314},"obj":"http://purl.obolibrary.org/obo/CLO_0001527"},{"id":"T329","span":{"begin":370,"end":371},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T330","span":{"begin":528,"end":529},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28]."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T319","span":{"begin":381,"end":386},"obj":"Chemical"}],"attributes":[{"id":"A319","pred":"chebi_id","subj":"T319","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"}],"text":"Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28]."}

{"project":"LitCovid-sentences","denotations":[{"id":"T366","span":{"begin":0,"end":66},"obj":"Sentence"},{"id":"T367","span":{"begin":67,"end":191},"obj":"Sentence"},{"id":"T368","span":{"begin":192,"end":323},"obj":"Sentence"},{"id":"T369","span":{"begin":324,"end":439},"obj":"Sentence"},{"id":"T370","span":{"begin":440,"end":648},"obj":"Sentence"},{"id":"T371","span":{"begin":649,"end":746},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28]."}

{"project":"2_test","denotations":[{"id":"32106462-2003928-69893332","span":{"begin":319,"end":321},"obj":"2003928"}],"text":"Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28]."}