| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T366 |
0-66 |
Sentence |
denotes |
Different amplification techniques require different temperatures. |
| T367 |
67-191 |
Sentence |
denotes |
Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. |
| T368 |
192-323 |
Sentence |
denotes |
This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. |
| T369 |
324-439 |
Sentence |
denotes |
The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. |
| T370 |
440-648 |
Sentence |
denotes |
However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. |
| T371 |
649-746 |
Sentence |
denotes |
This can be done by using different heater geometries [26,37] or using double-sided heating [28]. |
