Different amplification techniques require different temperatures. Based on Table 1 one can conclude that a higher temperature would also give a larger temperature gradient within the system. This can be disadvantageous for amplification reactions, as optimal denaturation temperatures are in the range 92 °C to 94 °C [77]. The denaturation in this research was done in a separate water bath, so this temperature gradient was circumvented. However, when on-chip denaturation and/or another amplification technique will be used, a second step will be the optimization of the heater in order to create better temperature uniformity within the system. This can be done by using different heater geometries [26,37] or using double-sided heating [28].