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PMC:7091888 / 25936-26543 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1054 92-97 Gene denotes hACE2 Gene:59272
1055 106-110 Gene denotes ACE2 Gene:59272
1056 210-215 Gene denotes hACE2 Gene:59272
1057 73-83 Species denotes SARS-CoV-2 Tax:2697049
1058 310-320 Species denotes SARS-CoV-2 Tax:2697049
1059 391-399 Species denotes SARS-CoV Tax:694009
1060 404-412 Species denotes MERS-CoV Tax:1335626
1061 491-496 Species denotes human Tax:9606
1062 243-260 Chemical denotes calcium phosphate MESH:C020243
1063 465-469 Chemical denotes FITC MESH:D016650
1064 156-160 CellLine denotes 293T CVCL:0063
1065 177-181 CellLine denotes 293T CVCL:0063

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T210 161-166 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T211 182-187 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T212 325-332 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T213 497-500 Body_part denotes IgG http://purl.org/sig/ont/fma/fma62872
T214 501-509 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T460 73-81 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T461 73-77 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T462 310-318 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T463 310-314 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T464 391-399 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T465 391-395 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T453 102-105 http://purl.obolibrary.org/obo/NCBITaxon_9397 denotes bat
T454 156-160 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T455 156-160 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T456 156-160 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T457 161-166 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T458 177-181 http://purl.obolibrary.org/obo/CLO_0050894 denotes 293T
T459 177-181 http://purl.obolibrary.org/obo/CLO_0051650 denotes 293T
T460 177-181 http://purl.obolibrary.org/obo/CLO_0052052 denotes 293T
T461 182-187 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T462 273-275 http://purl.obolibrary.org/obo/CLO_0001382 denotes 48
T463 481-485 http://purl.obolibrary.org/obo/NCBITaxon_9925 denotes goat
T464 491-500 http://purl.obolibrary.org/obo/CLO_0053695 denotes human IgG

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T134 243-260 Chemical denotes calcium phosphate http://purl.obolibrary.org/obo/CHEBI_77635|http://purl.obolibrary.org/obo/CHEBI_9679
T136 243-250 Chemical denotes calcium http://purl.obolibrary.org/obo/CHEBI_22984|http://purl.obolibrary.org/obo/CHEBI_29320
T138 251-260 Chemical denotes phosphate http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474
T142 325-332 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T143 465-469 Chemical denotes FITC http://purl.obolibrary.org/obo/CHEBI_37926
T144 549-556 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T149 0-167 Sentence denotes Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells.
T150 168-390 Sentence denotes Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature.
T151 391-444 Sentence denotes SARS-CoV and MERS-CoV RBDs were included as controls.
T152 445-607 Sentence denotes After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above.