PMC:7091888 / 25936-26543 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"1054","span":{"begin":92,"end":97},"obj":"Gene"},{"id":"1055","span":{"begin":106,"end":110},"obj":"Gene"},{"id":"1056","span":{"begin":210,"end":215},"obj":"Gene"},{"id":"1057","span":{"begin":73,"end":83},"obj":"Species"},{"id":"1058","span":{"begin":310,"end":320},"obj":"Species"},{"id":"1059","span":{"begin":391,"end":399},"obj":"Species"},{"id":"1060","span":{"begin":404,"end":412},"obj":"Species"},{"id":"1061","span":{"begin":491,"end":496},"obj":"Species"},{"id":"1062","span":{"begin":243,"end":260},"obj":"Chemical"},{"id":"1063","span":{"begin":465,"end":469},"obj":"Chemical"},{"id":"1064","span":{"begin":156,"end":160},"obj":"CellLine"},{"id":"1065","span":{"begin":177,"end":181},"obj":"CellLine"}],"attributes":[{"id":"A1054","pred":"tao:has_database_id","subj":"1054","obj":"Gene:59272"},{"id":"A1055","pred":"tao:has_database_id","subj":"1055","obj":"Gene:59272"},{"id":"A1056","pred":"tao:has_database_id","subj":"1056","obj":"Gene:59272"},{"id":"A1057","pred":"tao:has_database_id","subj":"1057","obj":"Tax:2697049"},{"id":"A1058","pred":"tao:has_database_id","subj":"1058","obj":"Tax:2697049"},{"id":"A1059","pred":"tao:has_database_id","subj":"1059","obj":"Tax:694009"},{"id":"A1060","pred":"tao:has_database_id","subj":"1060","obj":"Tax:1335626"},{"id":"A1061","pred":"tao:has_database_id","subj":"1061","obj":"Tax:9606"},{"id":"A1062","pred":"tao:has_database_id","subj":"1062","obj":"MESH:C020243"},{"id":"A1063","pred":"tao:has_database_id","subj":"1063","obj":"MESH:D016650"},{"id":"A1064","pred":"tao:has_database_id","subj":"1064","obj":"CVCL:0063"},{"id":"A1065","pred":"tao:has_database_id","subj":"1065","obj":"CVCL:0063"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T210","span":{"begin":161,"end":166},"obj":"Body_part"},{"id":"T211","span":{"begin":182,"end":187},"obj":"Body_part"},{"id":"T212","span":{"begin":325,"end":332},"obj":"Body_part"},{"id":"T213","span":{"begin":497,"end":500},"obj":"Body_part"},{"id":"T214","span":{"begin":501,"end":509},"obj":"Body_part"}],"attributes":[{"id":"A210","pred":"fma_id","subj":"T210","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A211","pred":"fma_id","subj":"T211","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A212","pred":"fma_id","subj":"T212","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A213","pred":"fma_id","subj":"T213","obj":"http://purl.org/sig/ont/fma/fma62872"},{"id":"A214","pred":"fma_id","subj":"T214","obj":"http://purl.org/sig/ont/fma/fma62871"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T460","span":{"begin":73,"end":81},"obj":"Disease"},{"id":"T461","span":{"begin":73,"end":77},"obj":"Disease"},{"id":"T462","span":{"begin":310,"end":318},"obj":"Disease"},{"id":"T463","span":{"begin":310,"end":314},"obj":"Disease"},{"id":"T464","span":{"begin":391,"end":399},"obj":"Disease"},{"id":"T465","span":{"begin":391,"end":395},"obj":"Disease"}],"attributes":[{"id":"A460","pred":"mondo_id","subj":"T460","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A461","pred":"mondo_id","subj":"T461","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A462","pred":"mondo_id","subj":"T462","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A463","pred":"mondo_id","subj":"T463","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A464","pred":"mondo_id","subj":"T464","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A465","pred":"mondo_id","subj":"T465","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T453","span":{"begin":102,"end":105},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T454","span":{"begin":156,"end":160},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T455","span":{"begin":156,"end":160},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T456","span":{"begin":156,"end":160},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T457","span":{"begin":161,"end":166},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T458","span":{"begin":177,"end":181},"obj":"http://purl.obolibrary.org/obo/CLO_0050894"},{"id":"T459","span":{"begin":177,"end":181},"obj":"http://purl.obolibrary.org/obo/CLO_0051650"},{"id":"T460","span":{"begin":177,"end":181},"obj":"http://purl.obolibrary.org/obo/CLO_0052052"},{"id":"T461","span":{"begin":182,"end":187},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T462","span":{"begin":273,"end":275},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T463","span":{"begin":481,"end":485},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9925"},{"id":"T464","span":{"begin":491,"end":500},"obj":"http://purl.obolibrary.org/obo/CLO_0053695"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T134","span":{"begin":243,"end":260},"obj":"Chemical"},{"id":"T136","span":{"begin":243,"end":250},"obj":"Chemical"},{"id":"T138","span":{"begin":251,"end":260},"obj":"Chemical"},{"id":"T142","span":{"begin":325,"end":332},"obj":"Chemical"},{"id":"T143","span":{"begin":465,"end":469},"obj":"Chemical"},{"id":"T144","span":{"begin":549,"end":556},"obj":"Chemical"}],"attributes":[{"id":"A134","pred":"chebi_id","subj":"T134","obj":"http://purl.obolibrary.org/obo/CHEBI_77635"},{"id":"A135","pred":"chebi_id","subj":"T134","obj":"http://purl.obolibrary.org/obo/CHEBI_9679"},{"id":"A136","pred":"chebi_id","subj":"T136","obj":"http://purl.obolibrary.org/obo/CHEBI_22984"},{"id":"A137","pred":"chebi_id","subj":"T136","obj":"http://purl.obolibrary.org/obo/CHEBI_29320"},{"id":"A138","pred":"chebi_id","subj":"T138","obj":"http://purl.obolibrary.org/obo/CHEBI_18367"},{"id":"A139","pred":"chebi_id","subj":"T138","obj":"http://purl.obolibrary.org/obo/CHEBI_26020"},{"id":"A140","pred":"chebi_id","subj":"T138","obj":"http://purl.obolibrary.org/obo/CHEBI_35780"},{"id":"A141","pred":"chebi_id","subj":"T138","obj":"http://purl.obolibrary.org/obo/CHEBI_43474"},{"id":"A142","pred":"chebi_id","subj":"T142","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A143","pred":"chebi_id","subj":"T143","obj":"http://purl.obolibrary.org/obo/CHEBI_37926"},{"id":"A144","pred":"chebi_id","subj":"T144","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}

{"project":"LitCovid-sentences","denotations":[{"id":"T149","span":{"begin":0,"end":167},"obj":"Sentence"},{"id":"T150","span":{"begin":168,"end":390},"obj":"Sentence"},{"id":"T151","span":{"begin":391,"end":444},"obj":"Sentence"},{"id":"T152","span":{"begin":445,"end":607},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Flow cytometry analysis was also performed to detect the binding between SARS-CoV-2 RBD and hACE2, or bat-ACE2 (bACE2), receptor in transiently transfected 293T cells. Briefly, 293T cells were transfected with hACE2 or bACE2 plasmid using the calcium phosphate method, and 48 h later, they were incubated with SARS-CoV-2 RBD protein at various concentrations for 30 min at room temperature. SARS-CoV and MERS-CoV RBDs were included as controls. After staining with FITC-conjugated goat anti-human IgG antibody (1:500; Thermo Fisher Scientific), the mixture was analyzed by flow cytometry as described above."}