PMC:7068163 / 5546-6961 JSONTXT

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[5] and a commercial test kit with kit-specific assays, Bavaria, February 2020\nReal-time RT-PCR system PCR efficiency (%)a, linearity (R2) Limit of detection (copies/reaction) Unspecific signals count in E gene assay in totalb Unspecific signals in E gene assay (%)b Run time (hours)\nQuantiTect Virus +Rox Vial kit (QIAGEN) ND ND 451/743(75/126 NC, 376/617 patient samples) 60.7 1:50\nSuperScript III One-step RT-PCR System with Platinum TaqDNA Polymerase (Invitrogen) 95 / 0,99c 50c 13/257(2/38 NC,11/219 patient samples) 5.1 1:28\nRealStar SARS-CoV-2 RT-PCR kit 1.0 (Altona) 125 / 0,97d 10d 0/111(0/38 NC, 0/73 patients samples) 0 2:15\nNC: negative control samples; ND: not determined.\na E = 10−1/slope − 1.\nb Indicated counts and percentage values of unspecific background signals in the SARS-CoV E gene assay are based on the total number of tested patient samples as well as the negative extraction and non-template controls.\nc Only for RdRp gene assays, tested with four replicates of SARS-CoV Frankfurt 1 RNA [6]; 10-fold serial dilutions were determined. 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