PMC:7045880 / 4939-5743 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"131","span":{"begin":92,"end":100},"obj":"Species"},{"id":"132","span":{"begin":650,"end":660},"obj":"Species"},{"id":"133","span":{"begin":213,"end":221},"obj":"Chemical"},{"id":"134","span":{"begin":223,"end":233},"obj":"Chemical"},{"id":"135","span":{"begin":234,"end":246},"obj":"Chemical"},{"id":"136","span":{"begin":396,"end":399},"obj":"Chemical"},{"id":"137","span":{"begin":406,"end":440},"obj":"Chemical"},{"id":"138","span":{"begin":442,"end":446},"obj":"Chemical"},{"id":"139","span":{"begin":492,"end":502},"obj":"Chemical"},{"id":"140","span":{"begin":503,"end":515},"obj":"Chemical"},{"id":"141","span":{"begin":83,"end":91},"obj":"Disease"}],"attributes":[{"id":"A131","pred":"tao:has_database_id","subj":"131","obj":"Tax:9606"},{"id":"A132","pred":"tao:has_database_id","subj":"132","obj":"Tax:2697049"},{"id":"A134","pred":"tao:has_database_id","subj":"134","obj":"MESH:D010406"},{"id":"A135","pred":"tao:has_database_id","subj":"135","obj":"MESH:D013307"},{"id":"A136","pred":"tao:has_database_id","subj":"136","obj":"MESH:D002245"},{"id":"A139","pred":"tao:has_database_id","subj":"139","obj":"MESH:D010406"},{"id":"A140","pred":"tao:has_database_id","subj":"140","obj":"MESH:D013307"},{"id":"A141","pred":"tao:has_database_id","subj":"141","obj":"MESH:C000657245"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T23","span":{"begin":341,"end":346},"obj":"Body_part"},{"id":"T24","span":{"begin":364,"end":369},"obj":"Body_part"},{"id":"T25","span":{"begin":482,"end":487},"obj":"Body_part"},{"id":"T26","span":{"begin":592,"end":596},"obj":"Body_part"}],"attributes":[{"id":"A23","pred":"fma_id","subj":"T23","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A24","pred":"fma_id","subj":"T24","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A25","pred":"fma_id","subj":"T25","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A26","pred":"fma_id","subj":"T26","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T5","span":{"begin":482,"end":487},"obj":"Body_part"}],"attributes":[{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T26","span":{"begin":83,"end":91},"obj":"Disease"},{"id":"T27","span":{"begin":650,"end":658},"obj":"Disease"}],"attributes":[{"id":"A26","pred":"mondo_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A27","pred":"mondo_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T41","span":{"begin":4,"end":9},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T42","span":{"begin":247,"end":250},"obj":"http://purl.obolibrary.org/obo/CLO_0053733"},{"id":"T43","span":{"begin":261,"end":265},"obj":"http://purl.obolibrary.org/obo/CLO_0001757"},{"id":"T44","span":{"begin":302,"end":305},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T45","span":{"begin":336,"end":340},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T46","span":{"begin":336,"end":340},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T47","span":{"begin":341,"end":346},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T48","span":{"begin":359,"end":363},"obj":"http://purl.obolibrary.org/obo/CLO_0009524"},{"id":"T49","span":{"begin":359,"end":363},"obj":"http://purl.obolibrary.org/obo/CLO_0050515"},{"id":"T50","span":{"begin":364,"end":369},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T51","span":{"begin":390,"end":394},"obj":"http://purl.obolibrary.org/obo/CLO_0002131"},{"id":"T52","span":{"begin":517,"end":522},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T53","span":{"begin":592,"end":596},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T54","span":{"begin":678,"end":679},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T16","span":{"begin":200,"end":211},"obj":"Chemical"},{"id":"T17","span":{"begin":223,"end":233},"obj":"Chemical"},{"id":"T19","span":{"begin":234,"end":246},"obj":"Chemical"},{"id":"T21","span":{"begin":396,"end":399},"obj":"Chemical"},{"id":"T22","span":{"begin":492,"end":502},"obj":"Chemical"},{"id":"T24","span":{"begin":503,"end":515},"obj":"Chemical"},{"id":"T26","span":{"begin":612,"end":620},"obj":"Chemical"}],"attributes":[{"id":"A16","pred":"chebi_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/CHEBI_33281"},{"id":"A17","pred":"chebi_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/CHEBI_17334"},{"id":"A18","pred":"chebi_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/CHEBI_51356"},{"id":"A19","pred":"chebi_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/CHEBI_17076"},{"id":"A20","pred":"chebi_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/CHEBI_58007"},{"id":"A21","pred":"chebi_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/CHEBI_16526"},{"id":"A22","pred":"chebi_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CHEBI_17334"},{"id":"A23","pred":"chebi_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CHEBI_51356"},{"id":"A24","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_17076"},{"id":"A25","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_58007"},{"id":"A26","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_10545"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}

{"project":"LitCovid-sentences","denotations":[{"id":"T48","span":{"begin":0,"end":101},"obj":"Sentence"},{"id":"T49","span":{"begin":102,"end":347},"obj":"Sentence"},{"id":"T50","span":{"begin":348,"end":516},"obj":"Sentence"},{"id":"T51","span":{"begin":517,"end":632},"obj":"Sentence"},{"id":"T52","span":{"begin":633,"end":804},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention."}