PMC:7039956 / 16554-17032 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7039956","sourcedb":"PMC","sourceid":"7039956","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7039956","text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 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