PMC:7039956 / 16554-17032 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"409","span":{"begin":368,"end":371},"obj":"Gene"},{"id":"410","span":{"begin":109,"end":117},"obj":"Chemical"},{"id":"411","span":{"begin":232,"end":248},"obj":"Chemical"}],"attributes":[{"id":"A409","pred":"tao:has_database_id","subj":"409","obj":"Gene:3815"},{"id":"A410","pred":"tao:has_database_id","subj":"410","obj":"MESH:D002857"},{"id":"A411","pred":"tao:has_database_id","subj":"411","obj":"MESH:D009841"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T306","span":{"begin":7,"end":11},"obj":"Body_part"},{"id":"T307","span":{"begin":12,"end":15},"obj":"Body_part"},{"id":"T308","span":{"begin":66,"end":70},"obj":"Body_part"},{"id":"T309","span":{"begin":159,"end":164},"obj":"Body_part"},{"id":"T310","span":{"begin":166,"end":171},"obj":"Body_part"},{"id":"T311","span":{"begin":363,"end":367},"obj":"Body_part"},{"id":"T312","span":{"begin":373,"end":375},"obj":"Body_part"}],"attributes":[{"id":"A306","pred":"fma_id","subj":"T306","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A307","pred":"fma_id","subj":"T307","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A308","pred":"fma_id","subj":"T308","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A309","pred":"fma_id","subj":"T309","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A310","pred":"fma_id","subj":"T310","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A311","pred":"fma_id","subj":"T311","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A312","pred":"fma_id","subj":"T312","obj":"http://purl.org/sig/ont/fma/fma13442"}],"text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T315","span":{"begin":7,"end":11},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T316","span":{"begin":66,"end":70},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T317","span":{"begin":159,"end":164},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T318","span":{"begin":166,"end":171},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T319","span":{"begin":298,"end":299},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T320","span":{"begin":363,"end":367},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T321","span":{"begin":373,"end":375},"obj":"http://purl.obolibrary.org/obo/CLO_0050428"}],"text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T17","span":{"begin":109,"end":117},"obj":"Chemical"},{"id":"T18","span":{"begin":232,"end":248},"obj":"Chemical"},{"id":"T19","span":{"begin":347,"end":355},"obj":"Chemical"}],"attributes":[{"id":"A17","pred":"chebi_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/CHEBI_28073"},{"id":"A18","pred":"chebi_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A19","pred":"chebi_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/CHEBI_28073"}],"text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System."}

{"project":"LitCovid-sentences","denotations":[{"id":"T104","span":{"begin":0,"end":46},"obj":"Sentence"},{"id":"T105","span":{"begin":47,"end":165},"obj":"Sentence"},{"id":"T106","span":{"begin":166,"end":403},"obj":"Sentence"},{"id":"T107","span":{"begin":404,"end":478},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Single cell RNA-sequencing library preparation\nTo generate single-cell Gel Beads-in-Emulsion (GEMs), the 10× chromium platform was used to capture and barcode cells. Cells were partitioned into GEMs along with gel beads coated with oligonucleotides and cDNAs with both barcodes were amplified, and a library was constructed using the 10× Genomics Chromium Single Cell Kit (v3 chemistry) for each sample. The resulting libraries were sequenced on an Illumina NovaSeq 6000 System."}