PMC:7039956 / 16025-16552 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"398","span":{"begin":285,"end":289},"obj":"Gene"},{"id":"399","span":{"begin":451,"end":460},"obj":"Gene"},{"id":"400","span":{"begin":462,"end":465},"obj":"Gene"},{"id":"401","span":{"begin":88,"end":92},"obj":"Gene"},{"id":"402","span":{"begin":218,"end":221},"obj":"Gene"},{"id":"403","span":{"begin":222,"end":227},"obj":"Species"},{"id":"404","span":{"begin":42,"end":47},"obj":"Chemical"},{"id":"405","span":{"begin":521,"end":526},"obj":"Chemical"}],"attributes":[{"id":"A398","pred":"tao:has_database_id","subj":"398","obj":"Gene:54453"},{"id":"A399","pred":"tao:has_database_id","subj":"399","obj":"Gene:308"},{"id":"A400","pred":"tao:has_database_id","subj":"400","obj":"Gene:3815"},{"id":"A401","pred":"tao:has_database_id","subj":"401","obj":"Gene:54453"},{"id":"A402","pred":"tao:has_database_id","subj":"402","obj":"Gene:3815"},{"id":"A403","pred":"tao:has_database_id","subj":"403","obj":"Tax:9606"},{"id":"A404","pred":"tao:has_database_id","subj":"404","obj":"MESH:C012939"},{"id":"A405","pred":"tao:has_database_id","subj":"405","obj":"MESH:C012939"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T293","span":{"begin":13,"end":20},"obj":"Body_part"},{"id":"T294","span":{"begin":93,"end":99},"obj":"Body_part"},{"id":"T295","span":{"begin":136,"end":140},"obj":"Body_part"},{"id":"T296","span":{"begin":180,"end":187},"obj":"Body_part"},{"id":"T297","span":{"begin":200,"end":204},"obj":"Body_part"},{"id":"T298","span":{"begin":327,"end":342},"obj":"Body_part"},{"id":"T299","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T300","span":{"begin":357,"end":371},"obj":"Body_part"},{"id":"T301","span":{"begin":361,"end":366},"obj":"Body_part"},{"id":"T302","span":{"begin":367,"end":371},"obj":"Body_part"},{"id":"T303","span":{"begin":402,"end":407},"obj":"Body_part"},{"id":"T304","span":{"begin":437,"end":441},"obj":"Body_part"},{"id":"T305","span":{"begin":487,"end":491},"obj":"Body_part"}],"attributes":[{"id":"A293","pred":"fma_id","subj":"T293","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A294","pred":"fma_id","subj":"T294","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A295","pred":"fma_id","subj":"T295","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A296","pred":"fma_id","subj":"T296","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A297","pred":"fma_id","subj":"T297","obj":"http://purl.org/sig/ont/fma/fma7163"},{"id":"A298","pred":"fma_id","subj":"T298","obj":"http://purl.org/sig/ont/fma/fma62845"},{"id":"A299","pred":"fma_id","subj":"T299","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A300","pred":"fma_id","subj":"T300","obj":"http://purl.org/sig/ont/fma/fma62845"},{"id":"A301","pred":"fma_id","subj":"T301","obj":"http://purl.org/sig/ont/fma/fma9670"},{"id":"A302","pred":"fma_id","subj":"T302","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A303","pred":"fma_id","subj":"T303","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A304","pred":"fma_id","subj":"T304","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A305","pred":"fma_id","subj":"T305","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T124","span":{"begin":93,"end":99},"obj":"Body_part"},{"id":"T125","span":{"begin":200,"end":204},"obj":"Body_part"},{"id":"T126","span":{"begin":331,"end":336},"obj":"Body_part"},{"id":"T127","span":{"begin":361,"end":366},"obj":"Body_part"}],"attributes":[{"id":"A124","pred":"uberon_id","subj":"T124","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A125","pred":"uberon_id","subj":"T125","obj":"http://purl.obolibrary.org/obo/UBERON_0000014"},{"id":"A126","pred":"uberon_id","subj":"T126","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A127","pred":"uberon_id","subj":"T127","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T47","span":{"begin":88,"end":92},"obj":"Disease"},{"id":"T48","span":{"begin":285,"end":289},"obj":"Disease"}],"attributes":[{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0100004"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0100004"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T302","span":{"begin":136,"end":140},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T303","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0000014"},{"id":"T304","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0001003"},{"id":"T305","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0002097"},{"id":"T306","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0002199"},{"id":"T307","span":{"begin":200,"end":204},"obj":"http://www.ebi.ac.uk/efo/EFO_0000962"},{"id":"T308","span":{"begin":222,"end":227},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T309","span":{"begin":283,"end":289},"obj":"http://purl.obolibrary.org/obo/CLO_0050087"},{"id":"T310","span":{"begin":327,"end":342},"obj":"http://purl.obolibrary.org/obo/CL_0000232"},{"id":"T311","span":{"begin":357,"end":371},"obj":"http://purl.obolibrary.org/obo/CL_0000232"},{"id":"T312","span":{"begin":402,"end":407},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T313","span":{"begin":437,"end":441},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T314","span":{"begin":487,"end":491},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T4","span":{"begin":100,"end":107},"obj":"http://purl.obolibrary.org/obo/GO_0051235"},{"id":"T5","span":{"begin":372,"end":377},"obj":"http://purl.obolibrary.org/obo/GO_0019835"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}

{"project":"LitCovid-sentences","denotations":[{"id":"T100","span":{"begin":0,"end":128},"obj":"Sentence"},{"id":"T101","span":{"begin":129,"end":316},"obj":"Sentence"},{"id":"T102","span":{"begin":317,"end":477},"obj":"Sentence"},{"id":"T103","span":{"begin":478,"end":527},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS."}