PMC:7039956 / 16025-16552
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7039956","sourcedb":"PMC","sourceid":"7039956","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7039956","text":"Fresh biopsy tissues were washed twice by D-PBS (Hyclone) and collected into prechilled MACS Tissue Storage Solution (Miltenyi). Single-cell suspensions were generated from biopsy tissues using Whole Skin Dissociation Kit human (Miltenyi) manufacturer guidelines and filtered by 70 μm MACS Smartstrainers (Miltenyi). Carryover red blood cells were lysed by Red Blood Cell Lysis Buffer (Abcam) and dead cells were removed by Easysep Dead Cell Removal (Annexin V) Kit (STEMCELL). Finally, cell pellets were re-suspended in D-PBS.","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"398","span":{"begin":285,"end":289},"obj":"Gene"},{"id":"399","span":{"begin":451,"end":460},"obj":"Gene"},{"id":"400","span":{"begin":462,"end":465},"obj":"Gene"},{"id":"401","span":{"begin":88,"end":92},"obj":"Gene"},{"id":"402","span":{"begin":218,"end":221},"obj":"Gene"},{"id":"403","span":{"begin":222,"end":227},"obj":"Species"},{"id":"404","span":{"begin":42,"end":47},"obj":"Chemical"},{"id":"405","span":{"begin":521,"end":526},"obj":"Chemical"}],"attributes":[{"id":"A398","pred":"tao:has_database_id","subj":"398","obj":"Gene:54453"},{"id":"A399","pred":"tao:has_database_id","subj":"399","obj":"Gene:308"},{"id":"A400","pred":"tao:has_database_id","subj":"400","obj":"Gene:3815"},{"id":"A401","pred":"tao:has_database_id","subj":"401","obj":"Gene:54453"},{"id":"A402","pred":"tao:has_database_id","subj":"402","obj":"Gene:3815"},{"id":"A403","pred":"tao:has_database_id","subj":"403","obj":"Tax:9606"},{"id":"A404","pred":"tao:has_database_id","subj":"404","obj":"MESH:C012939"},{"id":"A405","pred":"tao:has_database_id","subj":"405","obj":"MESH:C012939"},{"subj":"398","pred":"source","obj":"LitCovid-PubTator"},{"subj":"399","pred":"source","obj":"LitCovid-PubTator"},{"subj":"400","pred":"source","obj":"LitCovid-PubTator"},{"subj":"401","pred":"source","obj":"LitCovid-PubTator"},{"subj":"402","pred":"source","obj":"LitCovid-PubTator"},{"subj":"403","pred":"source","obj":"LitCovid-PubTator"},{"subj":"404","pred":"source","obj":"LitCovid-PubTator"},{"subj":"405","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T293","span":{"begin":13,"end":20},"obj":"Body_part"},{"id":"T294","span":{"begin":93,"end":99},"obj":"Body_part"},{"id":"T295","span":{"begin":136,"end":140},"obj":"Body_part"},{"id":"T296","span":{"begin":180,"end":187},"obj":"Body_part"},{"id":"T297","span":{"begin":200,"end":204},"obj":"Body_part"},{"id":"T298","span":{"begin":327,"end":342},"obj":"Body_part"},{"id":"T299","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T300","span":{"begin":357,"end":371},"obj":"Body_part"},{"id":"T301","span":{"begin":361,"end":366},"obj":"Body_part"},{"id":"T302","span":{"begin":367,"end":371},"obj":"Body_part"},{"id":"T303","span":{"begin":402,"end":407},"obj":"Body_part"},{"id":"T304","span":{"begin":437,"end":441},"obj":"Body_part"},{"id":"T305","span":{"begin":487,"end":491},"obj":"Body_part"}],"attributes":[{"id":"A293","pred":"fma_id","subj":"T293","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A294","pred":"fma_id","subj":"T294","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A295","pred":"fma_id","subj":"T295","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A296","pred":"fma_id","subj":"T296","obj":"http://purl.org/sig/ont/fma/fma9637"},{"id":"A297","pred":"fma_id","subj":"T297","obj":"http://purl.org/sig/ont/fma/fma7163"},{"id":"A298","pred":"fma_id","subj":"T298","obj":"http://purl.org/sig/ont/fma/fma62845"},{"id":"A299","pred":"fma_id","subj":"T299","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A300","pred":"fma_id","subj":"T300","obj":"http://purl.org/sig/ont/fma/fma62845"},{"id":"A301","pred":"fma_id","subj":"T301","obj":"http://purl.org/sig/ont/fma/fma9670"},{"id":"A302","pred":"fma_id","subj":"T302","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A303","pred":"fma_id","subj":"T303","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A304","pred":"fma_id","subj":"T304","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A305","pred":"fma_id","subj":"T305","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T293","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T294","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T295","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T296","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T297","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T298","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T299","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T300","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T301","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T302","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T303","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T304","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T305","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T124","span":{"begin":93,"end":99},"obj":"Body_part"},{"id":"T125","span":{"begin":200,"end":204},"obj":"Body_part"},{"id":"T126","span":{"begin":331,"end":336},"obj":"Body_part"},{"id":"T127","span":{"begin":361,"end":366},"obj":"Body_part"}],"attributes":[{"id":"A124","pred":"uberon_id","subj":"T124","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A125","pred":"uberon_id","subj":"T125","obj":"http://purl.obolibrary.org/obo/UBERON_0000014"},{"id":"A126","pred":"uberon_id","subj":"T126","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A127","pred":"uberon_id","subj":"T127","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"subj":"T124","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T125","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T126","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T127","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T47","span":{"begin":88,"end":92},"obj":"Disease"},{"id":"T48","span":{"begin":285,"end":289},"obj":"Disease"}],"attributes":[{"id":"A47","pred":"mondo_id","subj":"T47","obj":"http://purl.obolibrary.org/obo/MONDO_0100004"},{"id":"A48","pred":"mondo_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/MONDO_0100004"},{"subj":"T47","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T48","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T302","span":{"begin":136,"end":140},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T303","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0000014"},{"id":"T304","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0001003"},{"id":"T305","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0002097"},{"id":"T306","span":{"begin":200,"end":204},"obj":"http://purl.obolibrary.org/obo/UBERON_0002199"},{"id":"T307","span":{"begin":200,"end":204},"obj":"http://www.ebi.ac.uk/efo/EFO_0000962"},{"id":"T308","span":{"begin":222,"end":227},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T309","span":{"begin":283,"end":289},"obj":"http://purl.obolibrary.org/obo/CLO_0050087"},{"id":"T310","span":{"begin":327,"end":342},"obj":"http://purl.obolibrary.org/obo/CL_0000232"},{"id":"T311","span":{"begin":357,"end":371},"obj":"http://purl.obolibrary.org/obo/CL_0000232"},{"id":"T312","span":{"begin":402,"end":407},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T313","span":{"begin":437,"end":441},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T314","span":{"begin":487,"end":491},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"attributes":[{"subj":"T302","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T303","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T304","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T305","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T306","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T307","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T308","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T309","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T310","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T311","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T312","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T313","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T314","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T4","span":{"begin":100,"end":107},"obj":"http://purl.obolibrary.org/obo/GO_0051235"},{"id":"T5","span":{"begin":372,"end":377},"obj":"http://purl.obolibrary.org/obo/GO_0019835"}],"attributes":[{"subj":"T4","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T5","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T100","span":{"begin":0,"end":128},"obj":"Sentence"},{"id":"T101","span":{"begin":129,"end":316},"obj":"Sentence"},{"id":"T102","span":{"begin":317,"end":477},"obj":"Sentence"},{"id":"T103","span":{"begin":478,"end":527},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T100","pred":"source","obj":"LitCovid-sentences"},{"subj":"T101","pred":"source","obj":"LitCovid-sentences"},{"subj":"T102","pred":"source","obj":"LitCovid-sentences"},{"subj":"T103","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#ec93b0","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#93caec"},{"id":"LitCovid-PD-UBERON","color":"#e4ec93"},{"id":"LitCovid-PD-MONDO","color":"#da93ec"},{"id":"LitCovid-PD-CLO","color":"#93ecc0"},{"id":"LitCovid-PD-GO-BP","color":"#eca693"},{"id":"LitCovid-sentences","color":"#939aec"}]}]}}