PMC:7019868 / 13939-15069 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"247","span":{"begin":25,"end":29},"obj":"Species"},{"id":"253","span":{"begin":661,"end":664},"obj":"Gene"},{"id":"254","span":{"begin":77,"end":81},"obj":"Species"},{"id":"255","span":{"begin":204,"end":208},"obj":"Chemical"},{"id":"256","span":{"begin":289,"end":299},"obj":"Disease"},{"id":"257","span":{"begin":309,"end":316},"obj":"Disease"}],"attributes":[{"id":"A247","pred":"tao:has_database_id","subj":"247","obj":"Tax:28295"},{"id":"A253","pred":"tao:has_database_id","subj":"253","obj":"Gene:6236"},{"id":"A254","pred":"tao:has_database_id","subj":"254","obj":"Tax:28295"},{"id":"A256","pred":"tao:has_database_id","subj":"256","obj":"MESH:D006973"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T70","span":{"begin":408,"end":411},"obj":"Body_part"},{"id":"T71","span":{"begin":973,"end":976},"obj":"Body_part"},{"id":"T72","span":{"begin":1112,"end":1115},"obj":"Body_part"}],"attributes":[{"id":"A70","pred":"fma_id","subj":"T70","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A71","pred":"fma_id","subj":"T71","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A72","pred":"fma_id","subj":"T72","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T153","span":{"begin":97,"end":100},"obj":"http://purl.obolibrary.org/obo/CLO_0051582"},{"id":"T154","span":{"begin":297,"end":299},"obj":"http://purl.obolibrary.org/obo/CLO_0004265"},{"id":"T155","span":{"begin":633,"end":634},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T156","span":{"begin":768,"end":770},"obj":"http://purl.obolibrary.org/obo/CLO_0053799"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T80","span":{"begin":241,"end":253},"obj":"Chemical"},{"id":"T81","span":{"begin":249,"end":253},"obj":"Chemical"},{"id":"T82","span":{"begin":408,"end":411},"obj":"Chemical"},{"id":"T83","span":{"begin":599,"end":604},"obj":"Chemical"}],"attributes":[{"id":"A80","pred":"chebi_id","subj":"T80","obj":"http://purl.obolibrary.org/obo/CHEBI_33696"},{"id":"A81","pred":"chebi_id","subj":"T81","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"},{"id":"A82","pred":"chebi_id","subj":"T82","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A83","pred":"chebi_id","subj":"T83","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T30","span":{"begin":36,"end":50},"obj":"http://purl.obolibrary.org/obo/GO_0019076"},{"id":"T31","span":{"begin":82,"end":96},"obj":"http://purl.obolibrary.org/obo/GO_0019076"},{"id":"T32","span":{"begin":432,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T33","span":{"begin":440,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T34","span":{"begin":472,"end":493},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T35","span":{"begin":480,"end":493},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T36","span":{"begin":989,"end":1010},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T37","span":{"begin":997,"end":1010},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}

{"project":"LitCovid-sentences","denotations":[{"id":"T103","span":{"begin":0,"end":6},"obj":"Sentence"},{"id":"T104","span":{"begin":7,"end":50},"obj":"Sentence"},{"id":"T105","span":{"begin":51,"end":132},"obj":"Sentence"},{"id":"T106","span":{"begin":133,"end":393},"obj":"Sentence"},{"id":"T107","span":{"begin":394,"end":685},"obj":"Sentence"},{"id":"T108","span":{"begin":686,"end":823},"obj":"Sentence"},{"id":"T109","span":{"begin":824,"end":1063},"obj":"Sentence"},{"id":"T110","span":{"begin":1064,"end":1130},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"2.5.1. Quantification of PEDV Fecal Viral Shedding\nMethods to quantify fecal PEDV viral shedding has been described previously [19]. Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions. Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA). The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s. The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above. The detection limit was calculated as 4.8 log10 RNA copies per mL."}