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PMC:7019868 / 13939-15069 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
247 25-29 Species denotes PEDV Tax:28295
253 661-664 Gene denotes Rad Gene:6236
254 77-81 Species denotes PEDV Tax:28295
255 204-208 Chemical denotes DPBS
256 289-299 Disease denotes QIAcube HT MESH:D006973
257 309-316 Disease denotes QIAcube

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T70 408-411 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T71 973-976 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T72 1112-1115 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T153 97-100 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T154 297-299 http://purl.obolibrary.org/obo/CLO_0004265 denotes HT
T155 633-634 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T156 768-770 http://purl.obolibrary.org/obo/CLO_0053799 denotes 45

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T80 241-253 Chemical denotes nucleic acid http://purl.obolibrary.org/obo/CHEBI_33696
T81 249-253 Chemical denotes acid http://purl.obolibrary.org/obo/CHEBI_37527
T82 408-411 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T83 599-604 Chemical denotes probe http://purl.obolibrary.org/obo/CHEBI_50406

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T30 36-50 http://purl.obolibrary.org/obo/GO_0019076 denotes Viral Shedding
T31 82-96 http://purl.obolibrary.org/obo/GO_0019076 denotes viral shedding
T32 432-453 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse transcription
T33 440-453 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T34 472-493 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T35 480-493 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T36 989-1010 http://purl.obolibrary.org/obo/GO_0001171 denotes reverse transcription
T37 997-1010 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T103 0-6 Sentence denotes 2.5.1.
T104 7-50 Sentence denotes Quantification of PEDV Fecal Viral Shedding
T105 51-132 Sentence denotes Methods to quantify fecal PEDV viral shedding has been described previously [19].
T106 133-393 Sentence denotes Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions.
T107 394-685 Sentence denotes Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA).
T108 686-823 Sentence denotes The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s.
T109 824-1063 Sentence denotes The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above.
T110 1064-1130 Sentence denotes The detection limit was calculated as 4.8 log10 RNA copies per mL.