PMC:6988269 / 3935-8891 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/6988269","sourcedb":"PMC","sourceid":"6988269","source_url":"https://www.ncbi.nlm.nih.gov/pmc/6988269","text":"Methods\n\nClinical samples and coronavirus cell culture supernatants for initial assay evaluation\nCell culture supernatants containing typed coronaviruses and other respiratory viruses were provided by Charité and University of Hong Kong research laboratories. Respiratory samples were obtained during 2019 from patients hospitalised at Charité medical centre and tested by the NxTAG respiratory pathogen panel (Luminex, S´Hertogenbosch, The Netherlands) or in cases of MERS-CoV by the MERS-CoV upE assay as published before [10]. Additional samples were selected from biobanks at the Rijksinstituut voor Volksgezondheid en Milieu (RIVM), Bilthoven, at Erasmus University Medical Center, Rotterdam, at Public Health England (PHE), London, and at the University of Hong Kong. Samples from all collections comprised sputum as well as nose and throat swabs with or without viral transport medium.\nFaecal samples containing bat-derived SARS-related CoV samples (identified by GenBank accession numbers) were tested: KC633203, Betacoronavirus BtCoV/Rhi_eur/BB98–98/BGR/2008; KC633204, Betacoronavirus BtCoV/Rhi_eur/BB98–92/BGR/2008; KC633201, Betacoronavirus BtCoV/Rhi_bla/BB98–22/BGR/2008; GU190221 Betacoronavirus Bat coronavirus BR98–19/BGR/2008; GU190222 Betacoronavirus Bat coronavirus BM98–01/BGR/2008; GU190223, Betacoronavirus Bat coronavirus BM98–13/BGR/2008.\nAll synthetic RNA used in this study was photometrically quantified.\n\nRNA extraction\nRNA was extracted from clinical samples with the MagNA Pure 96 system (Roche, Penzberg, Germany) and from cell culture supernatants with the viral RNA mini kit (QIAGEN, Hilden, Germany).\n\nReal-time reverse-transcription PCR\nA 25 μL reaction contained 5 μL of RNA, 12.5 μL of 2 × reaction buffer provided with the Superscript III one step RT-PCR system with Platinum Taq Polymerase (Invitrogen, Darmstadt, Germany; containing 0.4 mM of each deoxyribont triphosphates (dNTP) and 3.2 mM magnesium sulphate), 1 μL of reverse transcriptase/Taq mixture from the kit, 0.4 μL of a 50 mM magnesium sulphate solution (Invitrogen), and 1 μg of nonacetylated bovine serum albumin (Roche). Primer and probe sequences, as well as optimised concentrations are shown in Table 1. All oligonucleotides were synthesised and provided by Tib-Molbiol (Berlin, Germany). Thermal cycling was performed at 55 °C for 10 min for reverse transcription, followed by 95 °C for 3 min and then 45 cycles of 95 °C for 15 s, 58 °C for 30 s. Participating laboratories used either Roche Light Cycler 480II or Applied Biosystems ViiA7 instruments (Applied Biosystems, Hong Kong, China).\nTable 1 Primers and probes, real-time RT-PCR for 2019 novel coronavirus\nAssay/use Oligonucleotide Sequencea Concentrationb\nRdRP gene RdRp_SARSr-F GTGARATGGTCATGTGTGGCGG Use 600 nM per reaction\nRdRp_SARSr-P2 FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ Specific for 2019-nCoV, will not detect SARS-CoV.Use 100 nM per reaction and mix with P1\nRdRP_SARSr-P1 FAM-CCAGGTGGWACRTCATCMGGTGATGC-BBQ Pan Sarbeco-Probe will detect 2019-nCoV, SARS-CoV and bat-SARS-related CoVs.Use 100 nM per reaction and mix with P2\nRdRp_SARSr-R CARATGTTAAASACACTATTAGCATA Use 800 nM per reaction\nE gene E_Sarbeco_F ACAGGTACGTTAATAGTTAATAGCGT Use 400 nm per reaction\nE_Sarbeco_P1 FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ Use 200 nm per reaction\nE_Sarbeco_R ATATTGCAGCAGTACGCACACA Use 400 nm per reaction\nN gene N_Sarbeco_F CACATTGGCACCCGCAATC Use 600 nm per reaction\nN_Sarbeco_P FAM-ACTTCCTCAAGGAACAACATTGCCA-BBQ Use 200 nm per reaction\nN_Sarbeco_R GAGGAACGAGAAGAGGCTTG Use 800 nm per reaction\na W is A/T; R is G/A; M is A/C; S is G/C. FAM: 6-carboxyfluorescein; BBQ: blackberry quencher.\nb Optimised concentrations are given in nanomol per litre (nM) based on the final reaction mix, e.g. 1.5 µL of a 10 µM primer stock solution per 25 µL total reaction volume yields a final concentration of 600 nM as indicated in the table.\n\nProtocol options and application notes\nLaboratories participating in the evaluation used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) with the same oligonucleotide concentrations and cycling conditions. The QIAGEN One-Step RT-PCR Kit was also tested and found to be compatible.\nThe intended cross-reactivity of all assays with viral RNA of SARS-CoV allows us to use the assays without having to rely on external sources of specific 2019-nCoV RNA.\nFor a routine workflow, we recommend the E gene assay as the first-line screening tool, followed by confirmatory testing with the RdRp gene assay. Application of the RdRp gene assay with dual colour technology can discriminate 2019-nCoV (both probes positive) from SARS-CoV RNA if the latter is used as positive control. Alternatively, laboratories may choose to run the RdRp assay with only the 2019-nCoV-specific probe.\n\nEthical statement\nThe internal use of samples for diagnostic workflow optimisation was agreed under the medical ethical rules of each of the participating partners.","divisions":[{"label":"title","span":{"begin":0,"end":7}},{"label":"sec","span":{"begin":9,"end":1431}},{"label":"title","span":{"begin":9,"end":96}},{"label":"p","span":{"begin":97,"end":892}},{"label":"p","span":{"begin":893,"end":1362}},{"label":"p","span":{"begin":1363,"end":1431}},{"label":"sec","span":{"begin":1433,"end":1634}},{"label":"title","span":{"begin":1433,"end":1447}},{"label":"p","span":{"begin":1448,"end":1634}},{"label":"sec","span":{"begin":1636,"end":3909}},{"label":"title","span":{"begin":1636,"end":1671}},{"label":"p","span":{"begin":1672,"end":2598}},{"label":"table-wrap","span":{"begin":2599,"end":3909}},{"label":"label","span":{"begin":2599,"end":2606}},{"label":"caption","span":{"begin":2608,"end":2671}},{"label":"title","span":{"begin":2608,"end":2671}},{"label":"table","span":{"begin":2672,"end":3575}},{"label":"tr","span":{"begin":2672,"end":2725}},{"label":"th","span":{"begin":2672,"end":2681}},{"label":"th","span":{"begin":2683,"end":2698}},{"label":"th","span":{"begin":2700,"end":2709}},{"label":"th","span":{"begin":2711,"end":2725}},{"label":"tr","span":{"begin":2726,"end":2798}},{"label":"td","span":{"begin":2726,"end":2735}},{"label":"td","span":{"begin":2737,"end":2749}},{"label":"td","span":{"begin":2751,"end":2773}},{"label":"td","span":{"begin":2775,"end":2798}},{"label":"tr","span":{"begin":2799,"end":2937}},{"label":"td","span":{"begin":2799,"end":2812}},{"label":"td","span":{"begin":2814,"end":2847}},{"label":"td","span":{"begin":2849,"end":2937}},{"label":"tr","span":{"begin":2938,"end":3104}},{"label":"td","span":{"begin":2938,"end":2951}},{"label":"td","span":{"begin":2953,"end":2987}},{"label":"td","span":{"begin":2989,"end":3104}},{"label":"tr","span":{"begin":3105,"end":3170}},{"label":"td","span":{"begin":3105,"end":3117}},{"label":"td","span":{"begin":3119,"end":3145}},{"label":"td","span":{"begin":3147,"end":3170}},{"label":"tr","span":{"begin":3171,"end":3243}},{"label":"td","span":{"begin":3171,"end":3177}},{"label":"td","span":{"begin":3179,"end":3190}},{"label":"td","span":{"begin":3192,"end":3218}},{"label":"td","span":{"begin":3220,"end":3243}},{"label":"tr","span":{"begin":3244,"end":3317}},{"label":"td","span":{"begin":3244,"end":3256}},{"label":"td","span":{"begin":3258,"end":3292}},{"label":"td","span":{"begin":3294,"end":3317}},{"label":"tr","span":{"begin":3318,"end":3378}},{"label":"td","span":{"begin":3318,"end":3329}},{"label":"td","span":{"begin":3331,"end":3353}},{"label":"td","span":{"begin":3355,"end":3378}},{"label":"tr","span":{"begin":3379,"end":3444}},{"label":"td","span":{"begin":3379,"end":3385}},{"label":"td","span":{"begin":3387,"end":3398}},{"label":"td","span":{"begin":3400,"end":3419}},{"label":"td","span":{"begin":3421,"end":3444}},{"label":"tr","span":{"begin":3445,"end":3516}},{"label":"td","span":{"begin":3445,"end":3456}},{"label":"td","span":{"begin":3458,"end":3491}},{"label":"td","span":{"begin":3493,"end":3516}},{"label":"tr","span":{"begin":3517,"end":3575}},{"label":"td","span":{"begin":3517,"end":3528}},{"label":"td","span":{"begin":3530,"end":3550}},{"label":"td","span":{"begin":3552,"end":3575}},{"label":"table-wrap-foot","span":{"begin":3576,"end":3909}},{"label":"p","span":{"begin":3576,"end":3671}},{"label":"p","span":{"begin":3671,"end":3910}},{"label":"sec","span":{"begin":3911,"end":4790}},{"label":"title","span":{"begin":3911,"end":3949}},{"label":"p","span":{"begin":3950,"end":4199}},{"label":"p","span":{"begin":4200,"end":4368}},{"label":"p","span":{"begin":4369,"end":4790}},{"label":"title","span":{"begin":4792,"end":4809}}],"tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T4","span":{"begin":42,"end":46},"obj":"Body_part"},{"id":"T5","span":{"begin":97,"end":101},"obj":"Body_part"},{"id":"T6","span":{"begin":813,"end":819},"obj":"Body_part"},{"id":"T7","span":{"begin":831,"end":835},"obj":"Body_part"},{"id":"T8","span":{"begin":840,"end":846},"obj":"Body_part"},{"id":"T9","span":{"begin":1377,"end":1380},"obj":"Body_part"},{"id":"T10","span":{"begin":1433,"end":1436},"obj":"Body_part"},{"id":"T11","span":{"begin":1448,"end":1451},"obj":"Body_part"},{"id":"T12","span":{"begin":1554,"end":1558},"obj":"Body_part"},{"id":"T13","span":{"begin":1595,"end":1598},"obj":"Body_part"},{"id":"T14","span":{"begin":1707,"end":1710},"obj":"Body_part"},{"id":"T15","span":{"begin":2102,"end":2107},"obj":"Body_part"},{"id":"T16","span":{"begin":2731,"end":2735},"obj":"Body_part"},{"id":"T17","span":{"begin":3173,"end":3177},"obj":"Body_part"},{"id":"T18","span":{"begin":3381,"end":3385},"obj":"Body_part"},{"id":"T19","span":{"begin":3605,"end":3609},"obj":"Body_part"},{"id":"T20","span":{"begin":4255,"end":4258},"obj":"Body_part"},{"id":"T21","span":{"begin":4364,"end":4367},"obj":"Body_part"},{"id":"T22","span":{"begin":4412,"end":4416},"obj":"Body_part"},{"id":"T23","span":{"begin":4504,"end":4508},"obj":"Body_part"},{"id":"T24","span":{"begin":4540,"end":4544},"obj":"Body_part"},{"id":"T25","span":{"begin":4643,"end":4646},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A5","pred":"fma_id","subj":"T5","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A6","pred":"fma_id","subj":"T6","obj":"http://purl.org/sig/ont/fma/fma312401"},{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma46472"},{"id":"A8","pred":"fma_id","subj":"T8","obj":"http://purl.org/sig/ont/fma/fma228738"},{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A10","pred":"fma_id","subj":"T10","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A11","pred":"fma_id","subj":"T11","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A12","pred":"fma_id","subj":"T12","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A13","pred":"fma_id","subj":"T13","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A14","pred":"fma_id","subj":"T14","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A15","pred":"fma_id","subj":"T15","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A16","pred":"fma_id","subj":"T16","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A17","pred":"fma_id","subj":"T17","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A18","pred":"fma_id","subj":"T18","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A19","pred":"fma_id","subj":"T19","obj":"http://purl.org/sig/ont/fma/fma284995"},{"id":"A20","pred":"fma_id","subj":"T20","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A21","pred":"fma_id","subj":"T21","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A22","pred":"fma_id","subj":"T22","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A23","pred":"fma_id","subj":"T23","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A24","pred":"fma_id","subj":"T24","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A25","pred":"fma_id","subj":"T25","obj":"http://purl.org/sig/ont/fma/fma67095"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T5","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T6","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T8","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T11","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T12","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T13","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T14","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T15","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T21","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T22","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T23","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T24","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T25","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T1","span":{"begin":813,"end":819},"obj":"Body_part"},{"id":"T2","span":{"begin":831,"end":835},"obj":"Body_part"},{"id":"T3","span":{"begin":840,"end":846},"obj":"Body_part"},{"id":"T4","span":{"begin":2102,"end":2107},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0007311"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000004"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000341"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"subj":"T1","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T2","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-UBERON"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T13","span":{"begin":931,"end":935},"obj":"Disease"},{"id":"T14","span":{"begin":2889,"end":2893},"obj":"Disease"},{"id":"T15","span":{"begin":3030,"end":3038},"obj":"Disease"},{"id":"T16","span":{"begin":3030,"end":3034},"obj":"Disease"},{"id":"T17","span":{"begin":3047,"end":3051},"obj":"Disease"},{"id":"T18","span":{"begin":4262,"end":4270},"obj":"Disease"},{"id":"T19","span":{"begin":4262,"end":4266},"obj":"Disease"},{"id":"T20","span":{"begin":4634,"end":4642},"obj":"Disease"},{"id":"T21","span":{"begin":4634,"end":4638},"obj":"Disease"}],"attributes":[{"id":"A13","pred":"mondo_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A14","pred":"mondo_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A15","pred":"mondo_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A16","pred":"mondo_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A17","pred":"mondo_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A18","pred":"mondo_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A19","pred":"mondo_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A20","pred":"mondo_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A21","pred":"mondo_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T13","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T14","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T15","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T21","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T33","span":{"begin":42,"end":46},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T34","span":{"begin":97,"end":101},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T35","span":{"begin":176,"end":183},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T36","span":{"begin":363,"end":369},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T37","span":{"begin":620,"end":622},"obj":"http://purl.obolibrary.org/obo/CLO_0037161"},{"id":"T38","span":{"begin":831,"end":835},"obj":"http://www.ebi.ac.uk/efo/EFO_0000828"},{"id":"T39","span":{"begin":919,"end":922},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T40","span":{"begin":1003,"end":1009},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T41","span":{"begin":1059,"end":1062},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T42","span":{"begin":1117,"end":1120},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T43","span":{"begin":1172,"end":1174},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T44","span":{"begin":1175,"end":1178},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T45","span":{"begin":1234,"end":1237},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T46","span":{"begin":1293,"end":1296},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T47","span":{"begin":1353,"end":1356},"obj":"http://purl.obolibrary.org/obo/PR_000001807"},{"id":"T48","span":{"begin":1554,"end":1558},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T49","span":{"begin":1672,"end":1673},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T50","span":{"begin":2019,"end":2020},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T51","span":{"begin":2278,"end":2284},"obj":"http://purl.obolibrary.org/obo/CLO_0001929"},{"id":"T52","span":{"begin":2410,"end":2412},"obj":"http://purl.obolibrary.org/obo/CLO_0053799"},{"id":"T53","span":{"begin":2547,"end":2558},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T54","span":{"begin":2731,"end":2735},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T55","span":{"begin":2810,"end":2812},"obj":"http://purl.obolibrary.org/obo/CLO_0008307"},{"id":"T56","span":{"begin":2935,"end":2937},"obj":"http://purl.obolibrary.org/obo/CLO_0008285"},{"id":"T57","span":{"begin":2949,"end":2951},"obj":"http://purl.obolibrary.org/obo/CLO_0008285"},{"id":"T58","span":{"begin":2989,"end":2992},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9596"},{"id":"T59","span":{"begin":3043,"end":3046},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T60","span":{"begin":3102,"end":3104},"obj":"http://purl.obolibrary.org/obo/CLO_0008307"},{"id":"T61","span":{"begin":3173,"end":3177},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T62","span":{"begin":3381,"end":3385},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T63","span":{"begin":3576,"end":3577},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T64","span":{"begin":3583,"end":3584},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T65","span":{"begin":3595,"end":3596},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T66","span":{"begin":3603,"end":3604},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T67","span":{"begin":3671,"end":3672},"obj":"http://purl.obolibrary.org/obo/CLO_0001021"},{"id":"T68","span":{"begin":3782,"end":3786},"obj":"http://purl.obolibrary.org/obo/CLO_0001550"},{"id":"T69","span":{"begin":3851,"end":3852},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T70","span":{"begin":4016,"end":4021},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T71","span":{"begin":4165,"end":4171},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T72","span":{"begin":4373,"end":4374},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T73","span":{"begin":4412,"end":4416},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T74","span":{"begin":4482,"end":4489},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T75","span":{"begin":4504,"end":4508},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T76","span":{"begin":4540,"end":4544},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"}],"attributes":[{"subj":"T33","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T34","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T35","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T37","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T38","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T39","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T40","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T41","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T42","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T43","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T44","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T45","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T46","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T47","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T48","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T49","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T50","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T51","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T52","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T53","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T54","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T55","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T56","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T57","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T58","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T59","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T60","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T61","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T62","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T63","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T64","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T65","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T66","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T67","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T68","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T69","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T70","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T71","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T72","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T73","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T74","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T75","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T76","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T5","span":{"begin":620,"end":622},"obj":"Chemical"},{"id":"T6","span":{"begin":724,"end":727},"obj":"Chemical"},{"id":"T7","span":{"begin":1736,"end":1742},"obj":"Chemical"},{"id":"T8","span":{"begin":1932,"end":1950},"obj":"Chemical"},{"id":"T9","span":{"begin":1932,"end":1941},"obj":"Chemical"},{"id":"T10","span":{"begin":1942,"end":1950},"obj":"Chemical"},{"id":"T11","span":{"begin":1987,"end":1994},"obj":"Chemical"},{"id":"T12","span":{"begin":2027,"end":2045},"obj":"Chemical"},{"id":"T13","span":{"begin":2027,"end":2036},"obj":"Chemical"},{"id":"T14","span":{"begin":2037,"end":2045},"obj":"Chemical"},{"id":"T15","span":{"begin":2046,"end":2054},"obj":"Chemical"},{"id":"T16","span":{"begin":2136,"end":2141},"obj":"Chemical"},{"id":"T17","span":{"begin":2215,"end":2231},"obj":"Chemical"},{"id":"T18","span":{"begin":2500,"end":2505},"obj":"Chemical"},{"id":"T19","span":{"begin":2683,"end":2698},"obj":"Chemical"},{"id":"T20","span":{"begin":2810,"end":2812},"obj":"Chemical"},{"id":"T21","span":{"begin":2935,"end":2937},"obj":"Chemical"},{"id":"T22","span":{"begin":2949,"end":2951},"obj":"Chemical"},{"id":"T23","span":{"begin":3102,"end":3104},"obj":"Chemical"},{"id":"T24","span":{"begin":3623,"end":3643},"obj":"Chemical"},{"id":"T25","span":{"begin":3803,"end":3811},"obj":"Chemical"},{"id":"T26","span":{"begin":3932,"end":3943},"obj":"Chemical"},{"id":"T27","span":{"begin":4070,"end":4085},"obj":"Chemical"},{"id":"T28","span":{"begin":4784,"end":4789},"obj":"Chemical"}],"attributes":[{"id":"A5","pred":"chebi_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CHEBI_30347"},{"id":"A6","pred":"chebi_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CHEBI_28044"},{"id":"A7","pred":"chebi_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A8","pred":"chebi_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CHEBI_32599"},{"id":"A9","pred":"chebi_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CHEBI_25107"},{"id":"A10","pred":"chebi_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CHEBI_16189"},{"id":"A11","pred":"chebi_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CHEBI_60004"},{"id":"A12","pred":"chebi_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/CHEBI_32599"},{"id":"A13","pred":"chebi_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/CHEBI_25107"},{"id":"A14","pred":"chebi_id","subj":"T14","obj":"http://purl.obolibrary.org/obo/CHEBI_16189"},{"id":"A15","pred":"chebi_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A16","pred":"chebi_id","subj":"T16","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"id":"A17","pred":"chebi_id","subj":"T17","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A18","pred":"chebi_id","subj":"T18","obj":"http://purl.obolibrary.org/obo/CHEBI_30212"},{"id":"A19","pred":"chebi_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A20","pred":"chebi_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CHEBI_33472"},{"id":"A21","pred":"chebi_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/CHEBI_60949"},{"id":"A22","pred":"chebi_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CHEBI_60949"},{"id":"A23","pred":"chebi_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/CHEBI_33472"},{"id":"A24","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_39073"},{"id":"A25","pred":"chebi_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/CHEBI_75958"},{"id":"A26","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_33232"},{"id":"A27","pred":"chebi_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/CHEBI_7754"},{"id":"A28","pred":"chebi_id","subj":"T28","obj":"http://purl.obolibrary.org/obo/CHEBI_50406"},{"subj":"T5","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T6","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T8","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T11","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T12","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T13","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T14","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T15","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T16","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T17","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T18","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T19","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T20","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T21","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T22","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T23","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T24","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T25","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T26","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T27","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T28","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T2","span":{"begin":869,"end":884},"obj":"http://purl.obolibrary.org/obo/GO_0046794"},{"id":"T3","span":{"begin":875,"end":884},"obj":"http://purl.obolibrary.org/obo/GO_0006810"},{"id":"T4","span":{"begin":1646,"end":1667},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T5","span":{"begin":1654,"end":1667},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T6","span":{"begin":1969,"end":1982},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T7","span":{"begin":1969,"end":1982},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T8","span":{"begin":2350,"end":2371},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T9","span":{"begin":2358,"end":2371},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T10","span":{"begin":2726,"end":2730},"obj":"http://purl.obolibrary.org/obo/GO_0003968"}],"attributes":[{"subj":"T2","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T5","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T6","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T8","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T9","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T10","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T31","span":{"begin":0,"end":7},"obj":"Sentence"},{"id":"T32","span":{"begin":9,"end":96},"obj":"Sentence"},{"id":"T33","span":{"begin":97,"end":259},"obj":"Sentence"},{"id":"T34","span":{"begin":260,"end":529},"obj":"Sentence"},{"id":"T35","span":{"begin":530,"end":773},"obj":"Sentence"},{"id":"T36","span":{"begin":774,"end":892},"obj":"Sentence"},{"id":"T37","span":{"begin":893,"end":1362},"obj":"Sentence"},{"id":"T38","span":{"begin":1363,"end":1431},"obj":"Sentence"},{"id":"T39","span":{"begin":1433,"end":1447},"obj":"Sentence"},{"id":"T40","span":{"begin":1448,"end":1634},"obj":"Sentence"},{"id":"T41","span":{"begin":1636,"end":1671},"obj":"Sentence"},{"id":"T42","span":{"begin":1672,"end":2124},"obj":"Sentence"},{"id":"T43","span":{"begin":2125,"end":2210},"obj":"Sentence"},{"id":"T44","span":{"begin":2211,"end":2295},"obj":"Sentence"},{"id":"T45","span":{"begin":2296,"end":2454},"obj":"Sentence"},{"id":"T46","span":{"begin":2455,"end":2598},"obj":"Sentence"},{"id":"T47","span":{"begin":2599,"end":2671},"obj":"Sentence"},{"id":"T48","span":{"begin":2672,"end":2725},"obj":"Sentence"},{"id":"T49","span":{"begin":2726,"end":2798},"obj":"Sentence"},{"id":"T50","span":{"begin":2799,"end":2937},"obj":"Sentence"},{"id":"T51","span":{"begin":2938,"end":3104},"obj":"Sentence"},{"id":"T52","span":{"begin":3105,"end":3170},"obj":"Sentence"},{"id":"T53","span":{"begin":3171,"end":3243},"obj":"Sentence"},{"id":"T54","span":{"begin":3244,"end":3317},"obj":"Sentence"},{"id":"T55","span":{"begin":3318,"end":3378},"obj":"Sentence"},{"id":"T56","span":{"begin":3379,"end":3444},"obj":"Sentence"},{"id":"T57","span":{"begin":3445,"end":3516},"obj":"Sentence"},{"id":"T58","span":{"begin":3517,"end":3575},"obj":"Sentence"},{"id":"T59","span":{"begin":3576,"end":3617},"obj":"Sentence"},{"id":"T60","span":{"begin":3618,"end":3622},"obj":"Sentence"},{"id":"T61","span":{"begin":3623,"end":3670},"obj":"Sentence"},{"id":"T62","span":{"begin":3671,"end":3909},"obj":"Sentence"},{"id":"T63","span":{"begin":3911,"end":3949},"obj":"Sentence"},{"id":"T64","span":{"begin":3950,"end":4124},"obj":"Sentence"},{"id":"T65","span":{"begin":4125,"end":4199},"obj":"Sentence"},{"id":"T66","span":{"begin":4200,"end":4368},"obj":"Sentence"},{"id":"T67","span":{"begin":4369,"end":4515},"obj":"Sentence"},{"id":"T68","span":{"begin":4516,"end":4689},"obj":"Sentence"},{"id":"T69","span":{"begin":4690,"end":4790},"obj":"Sentence"},{"id":"T70","span":{"begin":4792,"end":4809},"obj":"Sentence"},{"id":"T71","span":{"begin":4810,"end":4956},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T31","pred":"source","obj":"LitCovid-sentences"},{"subj":"T32","pred":"source","obj":"LitCovid-sentences"},{"subj":"T33","pred":"source","obj":"LitCovid-sentences"},{"subj":"T34","pred":"source","obj":"LitCovid-sentences"},{"subj":"T35","pred":"source","obj":"LitCovid-sentences"},{"subj":"T36","pred":"source","obj":"LitCovid-sentences"},{"subj":"T37","pred":"source","obj":"LitCovid-sentences"},{"subj":"T38","pred":"source","obj":"LitCovid-sentences"},{"subj":"T39","pred":"source","obj":"LitCovid-sentences"},{"subj":"T40","pred":"source","obj":"LitCovid-sentences"},{"subj":"T41","pred":"source","obj":"LitCovid-sentences"},{"subj":"T42","pred":"source","obj":"LitCovid-sentences"},{"subj":"T43","pred":"source","obj":"LitCovid-sentences"},{"subj":"T44","pred":"source","obj":"LitCovid-sentences"},{"subj":"T45","pred":"source","obj":"LitCovid-sentences"},{"subj":"T46","pred":"source","obj":"LitCovid-sentences"},{"subj":"T47","pred":"source","obj":"LitCovid-sentences"},{"subj":"T48","pred":"source","obj":"LitCovid-sentences"},{"subj":"T49","pred":"source","obj":"LitCovid-sentences"},{"subj":"T50","pred":"source","obj":"LitCovid-sentences"},{"subj":"T51","pred":"source","obj":"LitCovid-sentences"},{"subj":"T52","pred":"source","obj":"LitCovid-sentences"},{"subj":"T53","pred":"source","obj":"LitCovid-sentences"},{"subj":"T54","pred":"source","obj":"LitCovid-sentences"},{"subj":"T55","pred":"source","obj":"LitCovid-sentences"},{"subj":"T56","pred":"source","obj":"LitCovid-sentences"},{"subj":"T57","pred":"source","obj":"LitCovid-sentences"},{"subj":"T58","pred":"source","obj":"LitCovid-sentences"},{"subj":"T59","pred":"source","obj":"LitCovid-sentences"},{"subj":"T60","pred":"source","obj":"LitCovid-sentences"},{"subj":"T61","pred":"source","obj":"LitCovid-sentences"},{"subj":"T62","pred":"source","obj":"LitCovid-sentences"},{"subj":"T63","pred":"source","obj":"LitCovid-sentences"},{"subj":"T64","pred":"source","obj":"LitCovid-sentences"},{"subj":"T65","pred":"source","obj":"LitCovid-sentences"},{"subj":"T66","pred":"source","obj":"LitCovid-sentences"},{"subj":"T67","pred":"source","obj":"LitCovid-sentences"},{"subj":"T68","pred":"source","obj":"LitCovid-sentences"},{"subj":"T69","pred":"source","obj":"LitCovid-sentences"},{"subj":"T70","pred":"source","obj":"LitCovid-sentences"},{"subj":"T71","pred":"source","obj":"LitCovid-sentences"}]},{"project":"2_test","denotations":[{"id":"31992387-23041020-29325739","span":{"begin":525,"end":527},"obj":"23041020"}],"attributes":[{"subj":"31992387-23041020-29325739","pred":"source","obj":"2_test"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"71","span":{"begin":30,"end":41},"obj":"Species"},{"id":"80","span":{"begin":140,"end":153},"obj":"Species"},{"id":"81","span":{"begin":311,"end":319},"obj":"Species"},{"id":"82","span":{"begin":469,"end":477},"obj":"Species"},{"id":"83","span":{"begin":485,"end":493},"obj":"Species"},{"id":"84","span":{"begin":164,"end":175},"obj":"Species"},{"id":"85","span":{"begin":260,"end":271},"obj":"Species"},{"id":"86","span":{"begin":383,"end":394},"obj":"Species"},{"id":"87","span":{"begin":584,"end":619},"obj":"Disease"},{"id":"98","span":{"begin":931,"end":947},"obj":"Species"},{"id":"99","span":{"begin":1021,"end":1036},"obj":"Species"},{"id":"100","span":{"begin":1079,"end":1094},"obj":"Species"},{"id":"101","span":{"begin":1137,"end":1152},"obj":"Species"},{"id":"102","span":{"begin":1194,"end":1209},"obj":"Species"},{"id":"103","span":{"begin":1210,"end":1225},"obj":"Species"},{"id":"104","span":{"begin":1253,"end":1268},"obj":"Species"},{"id":"105","span":{"begin":1269,"end":1301},"obj":"Species"},{"id":"106","span":{"begin":1313,"end":1328},"obj":"Species"},{"id":"107","span":{"begin":1329,"end":1361},"obj":"Species"},{"id":"109","span":{"begin":1367,"end":1380},"obj":"Species"},{"id":"111","span":{"begin":1604,"end":1607},"obj":"Gene"},{"id":"115","span":{"begin":2102,"end":2115},"obj":"Gene"},{"id":"116","span":{"begin":2004,"end":2007},"obj":"Gene"},{"id":"117","span":{"begin":2215,"end":2231},"obj":"Chemical"},{"id":"125","span":{"begin":3093,"end":3096},"obj":"Gene"},{"id":"126","span":{"begin":2926,"end":2929},"obj":"Gene"},{"id":"127","span":{"begin":2862,"end":2871},"obj":"Species"},{"id":"128","span":{"begin":2889,"end":2897},"obj":"Species"},{"id":"129","span":{"begin":3019,"end":3028},"obj":"Species"},{"id":"130","span":{"begin":3030,"end":3038},"obj":"Species"},{"id":"131","span":{"begin":3047,"end":3064},"obj":"Species"},{"id":"133","span":{"begin":2649,"end":2671},"obj":"Species"},{"id":"135","span":{"begin":3623,"end":3643},"obj":"Chemical"},{"id":"137","span":{"begin":3762,"end":3765},"obj":"Gene"},{"id":"141","span":{"begin":4036,"end":4039},"obj":"Gene"},{"id":"142","span":{"begin":4152,"end":4155},"obj":"Gene"},{"id":"143","span":{"begin":4070,"end":4085},"obj":"Chemical"},{"id":"146","span":{"begin":4262,"end":4270},"obj":"Species"},{"id":"147","span":{"begin":4354,"end":4363},"obj":"Species"},{"id":"154","span":{"begin":4499,"end":4503},"obj":"Gene"},{"id":"155","span":{"begin":4535,"end":4539},"obj":"Gene"},{"id":"156","span":{"begin":4740,"end":4744},"obj":"Gene"},{"id":"157","span":{"begin":4596,"end":4605},"obj":"Species"},{"id":"158","span":{"begin":4634,"end":4642},"obj":"Species"},{"id":"159","span":{"begin":4765,"end":4774},"obj":"Species"}],"attributes":[{"id":"A71","pred":"tao:has_database_id","subj":"71","obj":"Tax:11118"},{"id":"A80","pred":"tao:has_database_id","subj":"80","obj":"Tax:11118"},{"id":"A81","pred":"tao:has_database_id","subj":"81","obj":"Tax:9606"},{"id":"A82","pred":"tao:has_database_id","subj":"82","obj":"Tax:1335626"},{"id":"A83","pred":"tao:has_database_id","subj":"83","obj":"Tax:1335626"},{"id":"A84","pred":"tao:has_database_id","subj":"84","obj":"Tax:1439707"},{"id":"A85","pred":"tao:has_database_id","subj":"85","obj":"Tax:1439707"},{"id":"A86","pred":"tao:has_database_id","subj":"86","obj":"Tax:1439707"},{"id":"A98","pred":"tao:has_database_id","subj":"98","obj":"Tax:694009"},{"id":"A99","pred":"tao:has_database_id","subj":"99","obj":"Tax:694002"},{"id":"A100","pred":"tao:has_database_id","subj":"100","obj":"Tax:694002"},{"id":"A101","pred":"tao:has_database_id","subj":"101","obj":"Tax:694002"},{"id":"A102","pred":"tao:has_database_id","subj":"102","obj":"Tax:694002"},{"id":"A103","pred":"tao:has_database_id","subj":"103","obj":"Tax:1508220"},{"id":"A104","pred":"tao:has_database_id","subj":"104","obj":"Tax:694002"},{"id":"A105","pred":"tao:has_database_id","subj":"105","obj":"Tax:864602"},{"id":"A106","pred":"tao:has_database_id","subj":"106","obj":"Tax:694002"},{"id":"A107","pred":"tao:has_database_id","subj":"107","obj":"Tax:864605"},{"id":"A109","pred":"tao:has_database_id","subj":"109","obj":"Tax:2086595"},{"id":"A111","pred":"tao:has_database_id","subj":"111","obj":"Gene:3815"},{"id":"A115","pred":"tao:has_database_id","subj":"115","obj":"Gene:213"},{"id":"A116","pred":"tao:has_database_id","subj":"116","obj":"Gene:3815"},{"id":"A117","pred":"tao:has_database_id","subj":"117","obj":"MESH:D009841"},{"id":"A125","pred":"tao:has_database_id","subj":"125","obj":"Gene:83881"},{"id":"A126","pred":"tao:has_database_id","subj":"126","obj":"Gene:83881"},{"id":"A127","pred":"tao:has_database_id","subj":"127","obj":"Tax:2697049"},{"id":"A128","pred":"tao:has_database_id","subj":"128","obj":"Tax:694009"},{"id":"A129","pred":"tao:has_database_id","subj":"129","obj":"Tax:2697049"},{"id":"A130","pred":"tao:has_database_id","subj":"130","obj":"Tax:694009"},{"id":"A131","pred":"tao:has_database_id","subj":"131","obj":"Tax:694009"},{"id":"A133","pred":"tao:has_database_id","subj":"133","obj":"Tax:2697049"},{"id":"A135","pred":"tao:has_database_id","subj":"135","obj":"MESH:C024098"},{"id":"A137","pred":"tao:has_database_id","subj":"137","obj":"Gene:83881"},{"id":"A141","pred":"tao:has_database_id","subj":"141","obj":"Gene:83881"},{"id":"A142","pred":"tao:has_database_id","subj":"142","obj":"Gene:3815"},{"id":"A143","pred":"tao:has_database_id","subj":"143","obj":"MESH:D009841"},{"id":"A146","pred":"tao:has_database_id","subj":"146","obj":"Tax:694009"},{"id":"A147","pred":"tao:has_database_id","subj":"147","obj":"Tax:2697049"},{"id":"A154","pred":"tao:has_database_id","subj":"154","obj":"Gene:43740578"},{"id":"A155","pred":"tao:has_database_id","subj":"155","obj":"Gene:43740578"},{"id":"A156","pred":"tao:has_database_id","subj":"156","obj":"Gene:43740578"},{"id":"A157","pred":"tao:has_database_id","subj":"157","obj":"Tax:2697049"},{"id":"A158","pred":"tao:has_database_id","subj":"158","obj":"Tax:694009"},{"id":"A159","pred":"tao:has_database_id","subj":"159","obj":"Tax:2697049"},{"subj":"71","pred":"source","obj":"LitCovid-PubTator"},{"subj":"80","pred":"source","obj":"LitCovid-PubTator"},{"subj":"81","pred":"source","obj":"LitCovid-PubTator"},{"subj":"82","pred":"source","obj":"LitCovid-PubTator"},{"subj":"83","pred":"source","obj":"LitCovid-PubTator"},{"subj":"84","pred":"source","obj":"LitCovid-PubTator"},{"subj":"85","pred":"source","obj":"LitCovid-PubTator"},{"subj":"86","pred":"source","obj":"LitCovid-PubTator"},{"subj":"87","pred":"source","obj":"LitCovid-PubTator"},{"subj":"98","pred":"source","obj":"LitCovid-PubTator"},{"subj":"99","pred":"source","obj":"LitCovid-PubTator"},{"subj":"100","pred":"source","obj":"LitCovid-PubTator"},{"subj":"101","pred":"source","obj":"LitCovid-PubTator"},{"subj":"102","pred":"source","obj":"LitCovid-PubTator"},{"subj":"103","pred":"source","obj":"LitCovid-PubTator"},{"subj":"104","pred":"source","obj":"LitCovid-PubTator"},{"subj":"105","pred":"source","obj":"LitCovid-PubTator"},{"subj":"106","pred":"source","obj":"LitCovid-PubTator"},{"subj":"107","pred":"source","obj":"LitCovid-PubTator"},{"subj":"109","pred":"source","obj":"LitCovid-PubTator"},{"subj":"111","pred":"source","obj":"LitCovid-PubTator"},{"subj":"115","pred":"source","obj":"LitCovid-PubTator"},{"subj":"116","pred":"source","obj":"LitCovid-PubTator"},{"subj":"117","pred":"source","obj":"LitCovid-PubTator"},{"subj":"125","pred":"source","obj":"LitCovid-PubTator"},{"subj":"126","pred":"source","obj":"LitCovid-PubTator"},{"subj":"127","pred":"source","obj":"LitCovid-PubTator"},{"subj":"128","pred":"source","obj":"LitCovid-PubTator"},{"subj":"129","pred":"source","obj":"LitCovid-PubTator"},{"subj":"130","pred":"source","obj":"LitCovid-PubTator"},{"subj":"131","pred":"source","obj":"LitCovid-PubTator"},{"subj":"133","pred":"source","obj":"LitCovid-PubTator"},{"subj":"135","pred":"source","obj":"LitCovid-PubTator"},{"subj":"137","pred":"source","obj":"LitCovid-PubTator"},{"subj":"141","pred":"source","obj":"LitCovid-PubTator"},{"subj":"142","pred":"source","obj":"LitCovid-PubTator"},{"subj":"143","pred":"source","obj":"LitCovid-PubTator"},{"subj":"146","pred":"source","obj":"LitCovid-PubTator"},{"subj":"147","pred":"source","obj":"LitCovid-PubTator"},{"subj":"154","pred":"source","obj":"LitCovid-PubTator"},{"subj":"155","pred":"source","obj":"LitCovid-PubTator"},{"subj":"156","pred":"source","obj":"LitCovid-PubTator"},{"subj":"157","pred":"source","obj":"LitCovid-PubTator"},{"subj":"158","pred":"source","obj":"LitCovid-PubTator"},{"subj":"159","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"MyTest","denotations":[{"id":"31992387-23041020-29325739","span":{"begin":525,"end":527},"obj":"23041020"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"31992387-23041020-29325739","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#cbec93","default":true},{"id":"LitCovid-PD-UBERON","color":"#ec93e5"},{"id":"LitCovid-PD-MONDO","color":"#93ecd8"},{"id":"LitCovid-PD-CLO","color":"#ecbe93"},{"id":"LitCovid-PD-CHEBI","color":"#a493ec"},{"id":"LitCovid-PD-GO-BP","color":"#9bec93"},{"id":"LitCovid-sentences","color":"#ec93b5"},{"id":"2_test","color":"#93cfec"},{"id":"LitCovid-PubTator","color":"#e9ec93"},{"id":"MyTest","color":"#d593ec"}]}]}}