PMC:3359311 / 7936-9426 JSONTXT

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However, treatment with the caspase-1 inhibitor z-YVAD-fmk (YVAD) did not decrease IL-6 secretion by airway epithelial cells (Fig. 3c).\n10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). 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