PMC:3317373 / 4309-4839 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3317373","sourcedb":"PMC","sourceid":"3317373","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3317373","text":"2.2. In Vitro Model of TBI Established by Scratch Injury\nAstrocyte scratch injury was performed as in a previous report [7]. Astrocytes were planted in 6-well plates and grown to confluence. The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap. Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group. 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