PMC:3291650 / 40331-41366 JSON TXT

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test3

{"project":"test3","denotations":[{"id":"T18656","span":{"begin":1001,"end":1010},"obj":"Gene_expression"},{"id":"T18655","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T18654","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T18653","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T18652","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T18651","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T18650","span":{"begin":428,"end":432},"obj":"Protein"},{"id":"T18649","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T18648","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T18647","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T18646","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T18645","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T18644","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T18680","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T18679","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T18678","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T18677","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T18676","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T18675","span":{"begin":428,"end":432},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19026","span":{"begin":569,"end":572},"obj":"http://www.uniprot.org/uniprot/P21580"},{"id":"T19025","span":{"begin":428,"end":432},"obj":"http://www.uniprot.org/uniprot/P05231"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T18684","span":{"begin":1023,"end":1027},"obj":"http://purl.obolibrary.org/obo/GO_0004422"},{"id":"T18683","span":{"begin":362,"end":366},"obj":"http://purl.obolibrary.org/obo/GO_0004422"},{"id":"T18682","span":{"begin":155,"end":164},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T18681","span":{"begin":20,"end":29},"obj":"http://purl.obolibrary.org/obo/GO_0009058"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T18688","span":{"begin":428,"end":432},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T18687","span":{"begin":424,"end":430},"obj":"http://purl.obolibrary.org/obo/GO_0005135"},{"id":"T18686","span":{"begin":1023,"end":1027},"obj":"http://purl.obolibrary.org/obo/GO_0004422"},{"id":"T18685","span":{"begin":362,"end":366},"obj":"http://purl.obolibrary.org/obo/GO_0004422"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

sentences

{"project":"sentences","denotations":[{"id":"T18668","span":{"begin":989,"end":1035},"obj":"Sentence"},{"id":"T18667","span":{"begin":920,"end":988},"obj":"Sentence"},{"id":"T18666","span":{"begin":845,"end":919},"obj":"Sentence"},{"id":"T18665","span":{"begin":782,"end":844},"obj":"Sentence"},{"id":"T18664","span":{"begin":713,"end":781},"obj":"Sentence"},{"id":"T18663","span":{"begin":641,"end":712},"obj":"Sentence"},{"id":"T18662","span":{"begin":574,"end":640},"obj":"Sentence"},{"id":"T18661","span":{"begin":506,"end":573},"obj":"Sentence"},{"id":"T18660","span":{"begin":434,"end":505},"obj":"Sentence"},{"id":"T18659","span":{"begin":368,"end":433},"obj":"Sentence"},{"id":"T18658","span":{"begin":39,"end":367},"obj":"Sentence"},{"id":"T18657","span":{"begin":0,"end":38},"obj":"Sentence"},{"id":"T262","span":{"begin":0,"end":38},"obj":"Sentence"},{"id":"T263","span":{"begin":39,"end":367},"obj":"Sentence"},{"id":"T264","span":{"begin":368,"end":433},"obj":"Sentence"},{"id":"T265","span":{"begin":434,"end":505},"obj":"Sentence"},{"id":"T266","span":{"begin":506,"end":573},"obj":"Sentence"},{"id":"T267","span":{"begin":574,"end":640},"obj":"Sentence"},{"id":"T268","span":{"begin":641,"end":712},"obj":"Sentence"},{"id":"T269","span":{"begin":713,"end":781},"obj":"Sentence"},{"id":"T270","span":{"begin":782,"end":844},"obj":"Sentence"},{"id":"T271","span":{"begin":845,"end":919},"obj":"Sentence"},{"id":"T272","span":{"begin":920,"end":988},"obj":"Sentence"},{"id":"T273","span":{"begin":989,"end":1035},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

simple1

{"project":"simple1","denotations":[{"id":"T18694","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T18693","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T18692","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T18691","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T18690","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T18689","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T19074","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T19073","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T19072","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T19071","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T19070","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T19069","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T19038","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T19037","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T19036","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T19035","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T19034","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T19033","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T19044","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T19043","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T19042","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T19041","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T19040","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T19039","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T19092","span":{"begin":39,"end":102},"obj":"Negative_regulation"},{"id":"T19091","span":{"begin":15,"end":29},"obj":"Entity"},{"id":"T19090","span":{"begin":428,"end":432},"obj":"Protein"},{"id":"T19089","span":{"begin":339,"end":366},"obj":"Protein"},{"id":"T19088","span":{"begin":1023,"end":1027},"obj":"Protein"},{"id":"T19087","span":{"begin":0,"end":3},"obj":"Protein"},{"id":"T19086","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T19085","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T19084","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T19083","span":{"begin":69,"end":74},"obj":"Entity"},{"id":"T19082","span":{"begin":971,"end":973},"obj":"Protein"},{"id":"T19081","span":{"begin":39,"end":48},"obj":"Protein"},{"id":"T19080","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T19079","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T19078","span":{"begin":34,"end":38},"obj":"Protein"},{"id":"T19077","span":{"begin":142,"end":154},"obj":"Entity"},{"id":"T19076","span":{"begin":81,"end":84},"obj":"Protein"},{"id":"T19075","span":{"begin":252,"end":264},"obj":"Entity"}],"relations":[{"id":"R14348","pred":"themeOf","subj":"T19081","obj":"T19092"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T19050","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T19049","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T19048","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T19047","span":{"begin":257,"end":277},"obj":"Protein"},{"id":"T19046","span":{"begin":155,"end":164},"obj":"Gene_expression"},{"id":"T19045","span":{"begin":142,"end":154},"obj":"Protein"},{"id":"T19053","span":{"begin":1001,"end":1010},"obj":"Gene_expression"},{"id":"T19052","span":{"begin":1023,"end":1027},"obj":"Protein"},{"id":"T19051","span":{"begin":246,"end":251},"obj":"Gene_expression"}],"relations":[{"id":"R14342","pred":"themeOf","subj":"T19045","obj":"T19046"},{"id":"R14343","pred":"themeOf","subj":"T19047","obj":"T19051"},{"id":"R14344","pred":"themeOf","subj":"T19052","obj":"T19053"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

testone

{"project":"testone","denotations":[{"id":"T18643","span":{"begin":1001,"end":1010},"obj":"Gene_expression"},{"id":"T18642","span":{"begin":775,"end":780},"obj":"Protein"},{"id":"T18641","span":{"begin":706,"end":711},"obj":"Protein"},{"id":"T18640","span":{"begin":635,"end":639},"obj":"Protein"},{"id":"T18639","span":{"begin":569,"end":572},"obj":"Protein"},{"id":"T18638","span":{"begin":500,"end":504},"obj":"Protein"},{"id":"T18637","span":{"begin":428,"end":432},"obj":"Protein"}],"text":"RNA isolation, cDNA synthesis and qPCR\nTotal RNA was extracted using Aurum Total RNA mini kit (BioRad) and reverse transcribed into cDNA with iScript cDNA synthesis kit (BioRad) according to the manufacturer's instructions. qPCR was performed by using SYBR Green I master mix I (Roche) in the Lightcycler 480 detection system (Roche) with the following primers: HPRT: 5′-AGTGTTGGATACAGGCCAGAC-3′ and 5′CGTGATTCAAATCCCTGAAGT-3′; IL-6: 5′-GAGGATACCACTCCCAACAGACC-3′ and 5′-AAGTGCATCATCGTTGTTCATACA-3′; IFNβ: 5′-TCAGAATGAGTGGTGGTTGC-3′ and 5′-GACCTTTCAAATGCAGTAGATTCA-3′; A20: 5′-AAACCAATGGTGATGGAAACTG-3′ and 5′-GTTGTCCCATTCGTCATTCC-3′; CCL2: 5′-TTAAAAACCTGGATCGGAACCAA-3′ and 5′-GCATTAGCTTCAGATTTACGGGT-3′; CXCL1: 5′-GAGCCTCTAACCAGTTCCAG-3′ and 5′-TGAGTGTGGCTATGACTTCG-3′ and IFNα4: 5′-TGATGAGCTACTACTGGTCAGC-3′ and 5′-GATCTCTTAGCACAAGGATGGC-3′. Primers were designed with PerlPrimer (http://perlprimer.sourceforge.net). Quantification was performed using the comparative CT method (ΔΔCT). Results are expressed relative to HPRT values."}

PMC:3291650 / 40331-41366 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3291650 / 40331-41366 JSON TXT