PMC:3062687 / 30984-31428
Annnotations
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21438 | 29-33 | P23396 | denotes | RPS3 |
T21439 | 65-69 | O14920 | denotes | IKKβ |
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21367 | 0-8 | NN | denotes | LC-MS/MS |
T21368 | 9-17 | NN | denotes | Analysis |
T21369 | 18-21 | NN | denotes | GST |
T21370 | 22-24 | CC | denotes | or |
T21371 | 25-33 | NN | denotes | GST-RPS3 |
T21372 | 34-37 | VB | denotes | was |
T21373 | 38-47 | VB | denotes | incubated |
T21374 | 48-52 | IN | denotes | with |
T21375 | 53-64 | JJ | denotes | recombinant |
T21376 | 65-69 | NN | denotes | IKKβ |
T21377 | 70-77 | NN | denotes | protein |
T21378 | 78-80 | IN | denotes | as |
T21379 | 81-90 | VB | denotes | described |
T21380 | 91-96 | IN | denotes | above |
T21381 | 97-99 | IN | denotes | in |
T21382 | 100-102 | DT | denotes | an |
T21383 | 103-105 | FW | denotes | in |
T21384 | 106-111 | FW | denotes | vitro |
T21385 | 112-118 | NN | denotes | kinase |
T21386 | 119-124 | NN | denotes | assay |
T21387 | 125-133 | NN | denotes | reaction |
T21388 | 134-143 | VB | denotes | conducted |
T21389 | 144-151 | IN | denotes | without |
T21390 | 152-161 | NN | denotes | 32P-γ-ATP |
T21391 | 162-170 | NN | denotes | labeling |
T21392 | 172-175 | DT | denotes | The |
T21393 | 176-184 | NN | denotes | reaction |
T21394 | 185-188 | VB | denotes | was |
T21395 | 189-198 | VB | denotes | separated |
T21396 | 199-201 | IN | denotes | by |
T21397 | 202-210 | NN | denotes | SDS-PAGE |
T21398 | 210-211 | -COMMA- | denotes | , |
T21399 | 212-215 | CC | denotes | and |
T21400 | 216-219 | DT | denotes | the |
T21401 | 220-227 | NN | denotes | protein |
T21402 | 228-231 | NN | denotes | gel |
T21403 | 232-235 | VB | denotes | was |
T21404 | 236-243 | VB | denotes | stained |
T21405 | 244-248 | IN | denotes | with |
T21406 | 249-258 | NNP | denotes | Colloidal |
T21407 | 259-263 | NNP | denotes | Blue |
T21408 | 264-265 | -LRB- | denotes | ( |
T21409 | 265-275 | NN | denotes | Invitrogen |
T21410 | 275-276 | -RRB- | denotes | ) |
T21411 | 278-281 | DT | denotes | The |
T21412 | 282-295 | VB | denotes | corresponding |
T21413 | 296-303 | NN | denotes | protein |
T21414 | 304-313 | NN | denotes | fragments |
T21415 | 314-318 | VB | denotes | were |
T21416 | 319-326 | VB | denotes | excised |
T21417 | 327-330 | CC | denotes | and |
T21418 | 331-340 | VB | denotes | subjected |
T21419 | 341-343 | TO | denotes | to |
T21420 | 344-351 | NN | denotes | trypsin |
T21421 | 352-361 | NN | denotes | digestion |
T21422 | 362-365 | CC | denotes | and |
T21423 | 366-374 | NN | denotes | LC-MS/MS |
T21424 | 375-377 | IN | denotes | at |
T21425 | 378-381 | DT | denotes | the |
T21426 | 382-386 | NNP | denotes | Yale |
T21427 | 387-393 | NNP | denotes | Cancer |
T21428 | 394-400 | NNP | denotes | Center |
T21429 | 401-405 | NNP | denotes | Mass |
T21430 | 406-418 | NNP | denotes | Spectrometry |
T21431 | 419-427 | NNP | denotes | Resource |
T21432 | 428-429 | -LRB- | denotes | ( |
T21433 | 429-432 | NNP | denotes | New |
T21434 | 433-438 | NNP | denotes | Haven |
T21435 | 438-439 | -COMMA- | denotes | , |
T21436 | 440-442 | NN | denotes | CT |
T21437 | 442-443 | -RRB- | denotes | ) |
R16993 | T21368 | T21367 | arg1Of | Analysis,LC-MS/MS |
R16994 | T21369 | T21370 | arg1Of | GST,or |
R16995 | T21370 | T21372 | arg1Of | or,was |
R16996 | T21370 | T21373 | arg2Of | or,incubated |
R16997 | T21371 | T21370 | arg2Of | GST-RPS3,or |
R16998 | T21373 | T21372 | arg2Of | incubated,was |
R16999 | T21373 | T21374 | arg1Of | incubated,with |
R17000 | T21373 | T21378 | arg1Of | incubated,as |
R17001 | T21377 | T21374 | arg2Of | protein,with |
R17002 | T21377 | T21375 | arg1Of | protein,recombinant |
R17003 | T21377 | T21376 | arg1Of | protein,IKKβ |
R17004 | T21379 | T21378 | arg2Of | described,as |
R17005 | T21379 | T21380 | arg1Of | described,above |
R17006 | T21379 | T21381 | arg1Of | described,in |
R17007 | T21384 | T21383 | arg1Of | vitro,in |
R17008 | T21387 | T21381 | arg2Of | reaction,in |
R17009 | T21387 | T21382 | arg1Of | reaction,an |
R17010 | T21387 | T21384 | arg1Of | reaction,vitro |
R17011 | T21387 | T21385 | arg1Of | reaction,kinase |
R17012 | T21387 | T21386 | arg1Of | reaction,assay |
R17013 | T21387 | T21388 | arg2Of | reaction,conducted |
R17014 | T21388 | T21389 | arg1Of | conducted,without |
R17015 | T21391 | T21389 | arg2Of | labeling,without |
R17016 | T21391 | T21390 | arg1Of | labeling,32P-γ-ATP |
R17017 | T21393 | T21392 | arg1Of | reaction,The |
R17018 | T21393 | T21394 | arg1Of | reaction,was |
R17019 | T21393 | T21395 | arg2Of | reaction,separated |
R17020 | T21395 | T21394 | arg2Of | separated,was |
R17021 | T21395 | T21399 | arg1Of | separated,and |
R17022 | T21397 | T21395 | arg1Of | SDS-PAGE,separated |
R17023 | T21397 | T21396 | arg2Of | SDS-PAGE,by |
R17024 | T21399 | T21398 | arg1Of | and,"," |
R17025 | T21402 | T21400 | arg1Of | gel,the |
R17026 | T21402 | T21401 | arg1Of | gel,protein |
R17027 | T21402 | T21403 | arg1Of | gel,was |
R17028 | T21402 | T21404 | arg2Of | gel,stained |
R17029 | T21404 | T21399 | arg2Of | stained,and |
R17030 | T21404 | T21403 | arg2Of | stained,was |
R17031 | T21404 | T21405 | arg1Of | stained,with |
R17032 | T21407 | T21405 | arg2Of | Blue,with |
R17033 | T21407 | T21406 | arg1Of | Blue,Colloidal |
R17034 | T21407 | T21408 | arg1Of | Blue,( |
R17035 | T21409 | T21408 | arg2Of | Invitrogen,( |
R17036 | T21410 | T21408 | arg3Of | ),( |
R17037 | T21414 | T21411 | arg1Of | fragments,The |
R17038 | T21414 | T21412 | arg1Of | fragments,corresponding |
R17039 | T21414 | T21413 | arg1Of | fragments,protein |
R17040 | T21414 | T21415 | arg1Of | fragments,were |
R17041 | T21414 | T21416 | arg2Of | fragments,excised |
R17042 | T21414 | T21418 | arg2Of | fragments,subjected |
R17043 | T21416 | T21417 | arg1Of | excised,and |
R17044 | T21417 | T21415 | arg2Of | and,were |
R17045 | T21418 | T21417 | arg2Of | subjected,and |
R17046 | T21418 | T21419 | arg1Of | subjected,to |
R17047 | T21421 | T21420 | arg1Of | digestion,trypsin |
R17048 | T21421 | T21422 | arg1Of | digestion,and |
R17049 | T21422 | T21419 | arg2Of | and,to |
R17050 | T21422 | T21424 | arg1Of | and,at |
R17051 | T21423 | T21422 | arg2Of | LC-MS/MS,and |
R17052 | T21431 | T21424 | arg2Of | Resource,at |
R17053 | T21431 | T21425 | arg1Of | Resource,the |
R17054 | T21431 | T21426 | arg1Of | Resource,Yale |
R17055 | T21431 | T21427 | arg1Of | Resource,Cancer |
R17056 | T21431 | T21428 | arg1Of | Resource,Center |
R17057 | T21431 | T21429 | arg1Of | Resource,Mass |
R17058 | T21431 | T21430 | arg1Of | Resource,Spectrometry |
R17059 | T21431 | T21432 | arg1Of | Resource,( |
R17060 | T21434 | T21432 | arg2Of | Haven,( |
R17061 | T21434 | T21433 | arg1Of | Haven,New |
R17062 | T21434 | T21435 | arg1Of | Haven,"," |
R17063 | T21436 | T21435 | arg2Of | CT,"," |
R17064 | T21437 | T21432 | arg3Of | ),( |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21440 | 0-8 | NNP | denotes | LC-MS/MS |
T21441 | 9-17 | NNP | denotes | Analysis |
T21442 | 18-21 | NNP | denotes | GST |
T21443 | 22-24 | CC | denotes | or |
T21444 | 25-33 | NNP | denotes | GST-RPS3 |
T21445 | 34-37 | VBD | denotes | was |
T21446 | 38-47 | VBN | denotes | incubated |
T21447 | 48-52 | IN | denotes | with |
T21448 | 53-64 | JJ | denotes | recombinant |
T21449 | 65-69 | NNP | denotes | IKKβ |
T21450 | 70-77 | NN | denotes | protein |
T21451 | 78-80 | IN | denotes | as |
T21452 | 81-90 | VBN | denotes | described |
T21453 | 91-96 | IN | denotes | above |
T21454 | 97-99 | IN | denotes | in |
T21455 | 100-102 | DT | denotes | an |
T21456 | 103-105 | IN | denotes | in |
T21457 | 106-111 | NN | denotes | vitro |
T21458 | 112-118 | NN | denotes | kinase |
T21459 | 119-124 | NN | denotes | assay |
T21460 | 125-133 | NN | denotes | reaction |
T21461 | 134-143 | VBN | denotes | conducted |
T21462 | 144-151 | IN | denotes | without |
T21463 | 152-161 | NNP | denotes | 32P-γ-ATP |
T21464 | 162-170 | VBG | denotes | labeling |
T21465 | 170-171 | . | denotes | . |
T21466 | 172-175 | DT | denotes | The |
T21467 | 176-184 | NN | denotes | reaction |
T21468 | 185-188 | VBD | denotes | was |
T21469 | 189-198 | VBN | denotes | separated |
T21470 | 199-201 | IN | denotes | by |
T21471 | 202-210 | NNP | denotes | SDS-PAGE |
T21472 | 210-211 | , | denotes | , |
T21473 | 212-215 | CC | denotes | and |
T21474 | 216-219 | DT | denotes | the |
T21475 | 220-227 | NN | denotes | protein |
T21476 | 228-231 | NN | denotes | gel |
T21477 | 232-235 | VBD | denotes | was |
T21478 | 236-243 | VBN | denotes | stained |
T21479 | 244-248 | IN | denotes | with |
T21480 | 249-258 | NNP | denotes | Colloidal |
T21481 | 259-263 | NNP | denotes | Blue |
T21482 | 264-265 | -LRB- | denotes | ( |
T21483 | 265-275 | NNP | denotes | Invitrogen |
T21484 | 275-276 | -RRB- | denotes | ) |
T21485 | 276-277 | . | denotes | . |
T21486 | 278-281 | DT | denotes | The |
T21487 | 282-295 | JJ | denotes | corresponding |
T21488 | 296-303 | NN | denotes | protein |
T21489 | 304-313 | NNS | denotes | fragments |
T21490 | 314-318 | VBD | denotes | were |
T21491 | 319-326 | VBN | denotes | excised |
T21492 | 327-330 | CC | denotes | and |
T21493 | 331-340 | VBN | denotes | subjected |
T21494 | 341-343 | TO | denotes | to |
T21495 | 344-351 | VB | denotes | trypsin |
T21496 | 352-361 | NN | denotes | digestion |
T21497 | 362-365 | CC | denotes | and |
T21498 | 366-374 | NN | denotes | LC-MS/MS |
T21499 | 375-377 | IN | denotes | at |
T21500 | 378-381 | DT | denotes | the |
T21501 | 382-386 | NNP | denotes | Yale |
T21502 | 387-393 | NNP | denotes | Cancer |
T21503 | 394-400 | NNP | denotes | Center |
T21504 | 401-405 | NNP | denotes | Mass |
T21505 | 406-418 | NNP | denotes | Spectrometry |
T21506 | 419-427 | NNP | denotes | Resource |
T21507 | 428-429 | -LRB- | denotes | ( |
T21508 | 429-432 | NNP | denotes | New |
T21509 | 433-438 | NNP | denotes | Haven |
T21510 | 438-439 | , | denotes | , |
T21511 | 440-442 | NNP | denotes | CT |
T21512 | 442-443 | -RRB- | denotes | ) |
T21513 | 443-444 | . | denotes | . |
R17065 | T21440 | T21442 | compound | LC-MS/MS,GST |
R17066 | T21441 | T21442 | compound | Analysis,GST |
R17067 | T21442 | T21446 | nsubjpass | GST,incubated |
R17068 | T21443 | T21442 | cc | or,GST |
R17069 | T21444 | T21442 | conj | GST-RPS3,GST |
R17070 | T21445 | T21446 | auxpass | was,incubated |
R17071 | T21446 | T21446 | ROOT | incubated,incubated |
R17072 | T21447 | T21446 | prep | with,incubated |
R17073 | T21448 | T21450 | compound | recombinant,protein |
R17074 | T21449 | T21450 | compound | IKKβ,protein |
R17075 | T21450 | T21447 | pobj | protein,with |
R17076 | T21451 | T21452 | mark | as,described |
R17077 | T21452 | T21446 | advcl | described,incubated |
R17078 | T21453 | T21452 | advmod | above,described |
R17079 | T21454 | T21452 | prep | in,described |
R17080 | T21455 | T21454 | pcomp | an,in |
R17081 | T21456 | T21455 | prep | in,an |
R17082 | T21457 | T21460 | amod | vitro,reaction |
R17083 | T21458 | T21460 | amod | kinase,reaction |
R17084 | T21459 | T21460 | compound | assay,reaction |
R17085 | T21460 | T21456 | pobj | reaction,in |
R17086 | T21461 | T21460 | acl | conducted,reaction |
R17087 | T21462 | T21461 | prep | without,conducted |
R17088 | T21463 | T21464 | compound | 32P-γ-ATP,labeling |
R17089 | T21464 | T21462 | pobj | labeling,without |
R17090 | T21465 | T21446 | punct | .,incubated |
R17091 | T21466 | T21467 | det | The,reaction |
R17092 | T21467 | T21469 | nsubjpass | reaction,separated |
R17093 | T21468 | T21469 | auxpass | was,separated |
R17094 | T21469 | T21469 | ROOT | separated,separated |
R17095 | T21470 | T21469 | agent | by,separated |
R17096 | T21471 | T21470 | pobj | SDS-PAGE,by |
R17097 | T21472 | T21469 | punct | ",",separated |
R17098 | T21473 | T21469 | cc | and,separated |
R17099 | T21474 | T21476 | det | the,gel |
R17100 | T21475 | T21476 | compound | protein,gel |
R17101 | T21476 | T21478 | nsubjpass | gel,stained |
R17102 | T21477 | T21478 | auxpass | was,stained |
R17103 | T21478 | T21469 | conj | stained,separated |
R17104 | T21479 | T21478 | prep | with,stained |
R17105 | T21480 | T21481 | compound | Colloidal,Blue |
R17106 | T21481 | T21479 | pobj | Blue,with |
R17107 | T21482 | T21481 | punct | (,Blue |
R17108 | T21483 | T21481 | appos | Invitrogen,Blue |
R17109 | T21484 | T21481 | punct | ),Blue |
R17110 | T21485 | T21478 | punct | .,stained |
R17111 | T21486 | T21489 | det | The,fragments |
R17112 | T21487 | T21489 | amod | corresponding,fragments |
R17113 | T21488 | T21489 | compound | protein,fragments |
R17114 | T21489 | T21491 | nsubjpass | fragments,excised |
R17115 | T21490 | T21491 | auxpass | were,excised |
R17116 | T21491 | T21491 | ROOT | excised,excised |
R17117 | T21492 | T21491 | cc | and,excised |
R17118 | T21493 | T21491 | conj | subjected,excised |
R17119 | T21494 | T21495 | aux | to,trypsin |
R17120 | T21495 | T21493 | xcomp | trypsin,subjected |
R17121 | T21496 | T21495 | dobj | digestion,trypsin |
R17122 | T21497 | T21496 | cc | and,digestion |
R17123 | T21498 | T21496 | conj | LC-MS/MS,digestion |
R17124 | T21499 | T21495 | prep | at,trypsin |
R17125 | T21500 | T21503 | det | the,Center |
R17126 | T21501 | T21503 | compound | Yale,Center |
R17127 | T21502 | T21503 | compound | Cancer,Center |
R17128 | T21503 | T21506 | compound | Center,Resource |
R17129 | T21504 | T21506 | compound | Mass,Resource |
R17130 | T21505 | T21506 | compound | Spectrometry,Resource |
R17131 | T21506 | T21499 | pobj | Resource,at |
R17132 | T21507 | T21506 | punct | (,Resource |
R17133 | T21508 | T21509 | compound | New,Haven |
R17134 | T21509 | T21506 | appos | Haven,Resource |
R17135 | T21510 | T21509 | punct | ",",Haven |
R17136 | T21511 | T21509 | conj | CT,Haven |
R17137 | T21512 | T21506 | punct | ),Resource |
R17138 | T21513 | T21491 | punct | .,excised |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21520 | 352-361 | http://purl.obolibrary.org/obo/GO_0007586 | denotes | digestion |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21363 | 0-17 | Sentence | denotes | LC-MS/MS Analysis |
T21364 | 18-171 | Sentence | denotes | GST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. |
T21365 | 172-277 | Sentence | denotes | The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). |
T21366 | 278-444 | Sentence | denotes | The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT). |
T213 | 0-17 | Sentence | denotes | LC-MS/MS Analysis |
T214 | 18-171 | Sentence | denotes | GST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. |
T215 | 172-277 | Sentence | denotes | The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). |
T216 | 278-444 | Sentence | denotes | The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT). |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21532 | 25-33 | Protein | denotes | GST-RPS3 |
T21533 | 65-69 | Protein | denotes | IKKβ |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21521 | 18-21 | Protein | denotes | GST |
T21522 | 25-28 | Protein | denotes | GST |
T21523 | 29-33 | Protein | denotes | RPS3 |
T21524 | 53-77 | Protein | denotes | recombinant IKKβ protein |
T21525 | 344-351 | Protein | denotes | trypsin |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21361 | 25-33 | Protein | denotes | GST-RPS3 |
T21362 | 65-69 | Protein | denotes | IKKβ |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T21359 | 25-33 | Protein | denotes | GST-RPS3 |
T21360 | 65-69 | Protein | denotes | IKKβ |