PMC:3062687 / 30984-31428
Annnotations
bionlp-st-ge-2016-uniprot
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pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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GO-BP
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sentences
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events-check-again
{"project":"events-check-again","denotations":[{"id":"T21532","span":{"begin":25,"end":33},"obj":"Protein"},{"id":"T21533","span":{"begin":65,"end":69},"obj":"Protein"}],"text":"LC-MS/MS Analysis\nGST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT)."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T21521","span":{"begin":18,"end":21},"obj":"Protein"},{"id":"T21522","span":{"begin":25,"end":28},"obj":"Protein"},{"id":"T21523","span":{"begin":29,"end":33},"obj":"Protein"},{"id":"T21524","span":{"begin":53,"end":77},"obj":"Protein"},{"id":"T21525","span":{"begin":344,"end":351},"obj":"Protein"}],"text":"LC-MS/MS Analysis\nGST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT)."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T21361","span":{"begin":25,"end":33},"obj":"Protein"},{"id":"T21362","span":{"begin":65,"end":69},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"LC-MS/MS Analysis\nGST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT)."}
test2
{"project":"test2","denotations":[{"id":"T21359","span":{"begin":25,"end":33},"obj":"Protein"},{"id":"T21360","span":{"begin":65,"end":69},"obj":"Protein"}],"text":"LC-MS/MS Analysis\nGST or GST-RPS3 was incubated with recombinant IKKβ protein as described above in an in vitro kinase assay reaction conducted without 32P-γ-ATP labeling. The reaction was separated by SDS-PAGE, and the protein gel was stained with Colloidal Blue (Invitrogen). The corresponding protein fragments were excised and subjected to trypsin digestion and LC-MS/MS at the Yale Cancer Center Mass Spectrometry Resource (New Haven, CT)."}