PMC:2889865 / 29369-30082 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2889865","sourcedb":"PMC","sourceid":"2889865","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2889865","text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. 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