PMC:2889865 / 29369-30082 JSON TXT

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pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T13746","span":{"begin":529,"end":533},"obj":"http://purl.obolibrary.org/obo/GO_0007568"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T13747","span":{"begin":121,"end":125},"obj":"http://purl.obolibrary.org/obo/GO_0005138"},{"id":"T13748","span":{"begin":158,"end":162},"obj":"http://purl.obolibrary.org/obo/GO_0005138"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T13749","span":{"begin":103,"end":108},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13750","span":{"begin":323,"end":328},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13751","span":{"begin":499,"end":504},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13752","span":{"begin":547,"end":552},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

sentences

{"project":"sentences","denotations":[{"id":"T13450","span":{"begin":0,"end":41},"obj":"Sentence"},{"id":"T13451","span":{"begin":42,"end":304},"obj":"Sentence"},{"id":"T13452","span":{"begin":305,"end":468},"obj":"Sentence"},{"id":"T13453","span":{"begin":469,"end":588},"obj":"Sentence"},{"id":"T13454","span":{"begin":589,"end":657},"obj":"Sentence"},{"id":"T13455","span":{"begin":658,"end":713},"obj":"Sentence"},{"id":"T213","span":{"begin":0,"end":41},"obj":"Sentence"},{"id":"T214","span":{"begin":42,"end":304},"obj":"Sentence"},{"id":"T215","span":{"begin":305,"end":468},"obj":"Sentence"},{"id":"T216","span":{"begin":469,"end":588},"obj":"Sentence"},{"id":"T217","span":{"begin":589,"end":657},"obj":"Sentence"},{"id":"T218","span":{"begin":658,"end":713},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T13778","span":{"begin":121,"end":125},"obj":"Protein"},{"id":"T13779","span":{"begin":127,"end":132},"obj":"Protein"},{"id":"T13780","span":{"begin":158,"end":162},"obj":"Protein"},{"id":"T13781","span":{"begin":190,"end":195},"obj":"Protein"},{"id":"T13782","span":{"begin":226,"end":229},"obj":"Protein"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T13783","span":{"begin":121,"end":125},"obj":"Protein"},{"id":"T13784","span":{"begin":127,"end":132},"obj":"Protein"},{"id":"T13785","span":{"begin":137,"end":140},"obj":"Protein"},{"id":"T13786","span":{"begin":142,"end":172},"obj":"Protein"},{"id":"T13787","span":{"begin":174,"end":205},"obj":"Protein"},{"id":"T13788","span":{"begin":210,"end":239},"obj":"Protein"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T13445","span":{"begin":121,"end":125},"obj":"Protein"},{"id":"T13446","span":{"begin":127,"end":132},"obj":"Protein"},{"id":"T13447","span":{"begin":158,"end":162},"obj":"Protein"},{"id":"T13448","span":{"begin":190,"end":195},"obj":"Protein"},{"id":"T13449","span":{"begin":226,"end":229},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T13734","span":{"begin":121,"end":125},"obj":"P05231"},{"id":"T13735","span":{"begin":127,"end":132},"obj":"P10145"},{"id":"T13736","span":{"begin":137,"end":140},"obj":"P01375"},{"id":"T13737","span":{"begin":158,"end":162},"obj":"P05231"},{"id":"T13738","span":{"begin":190,"end":195},"obj":"P10145"},{"id":"T13739","span":{"begin":226,"end":229},"obj":"P01375"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

test2

{"project":"test2","denotations":[{"id":"T13440","span":{"begin":121,"end":125},"obj":"Protein"},{"id":"T13441","span":{"begin":127,"end":132},"obj":"Protein"},{"id":"T13442","span":{"begin":158,"end":162},"obj":"Protein"},{"id":"T13443","span":{"begin":190,"end":195},"obj":"Protein"},{"id":"T13444","span":{"begin":226,"end":229},"obj":"Protein"}],"text":"Enzyme-linked immunosorbent assay (ELISA)\nELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-6, CXCL8 and TNF (BD OptEIA Human IL-6 Elisa Set, BD OptEIA Human CXCL8 Elisa Set and BD OptEIA Human TNF Elisa Set, Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were stimulated with PMA (162 nM) for 1 h, centrifuged (1000 × g, 8 min) and the supernatants were collected and stored at -80°C until use. Following centrifugation, the cells were resuspended in 1 h aged media, where cells have been grown in, containing PMA. The same procedure was performed to collect media after 2 h and 6 h. The final collection of media was performed after 24 h."}

PMC:2889865 / 29369-30082 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2889865 / 29369-30082 JSON TXT