PMC:2848222 / 5034-5379 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2848222","sourcedb":"PMC","sourceid":"2848222","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2848222","text":"DNA extraction\nFirst, 200-μL cell lysis solution and 20-μl proteinase K stock solution were added to the tissue samples and incubated for 1 hour at 60°C, then for 20 minutes at 70°C. Subsequently, DNA was extracted after 72 hours at 37°C, protein was removed, and the DNA was precipitated using 100% 2-propanol and dissolved in hydration buffer.","divisions":[{"label":"title","span":{"begin":0,"end":14}}],"tracks":[]}