DNA extraction
First, 200-μL cell lysis solution and 20-μl proteinase K stock solution were added to the tissue samples and incubated for 1 hour at 60°C, then for 20 minutes at 70°C. Subsequently, DNA was extracted after 72 hours at 37°C, protein was removed, and the DNA was precipitated using 100% 2-propanol and dissolved in hydration buffer.