PMC:2728203 / 42046-42581 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2728203","sourcedb":"PMC","sourceid":"2728203","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2728203","text":"Strain/Plasmid Description Source/Reference\nYeast strains\nW303(ACY233) MATαura3Δ leu2Δ trp1Δ his3Δ Winston et al.47\nMLP1-TAP (ACY984) MATa his3Δ leu2Δ met15Δ ura3Δ MLP1-TAP::HIS3 Open Biosystems\nMLP1Δ (ACY481) MATa leu2Δ ura3Δ his3Δ MLP1::HIS3 Euroscarf\n\u2028\u2028\nPlasmids\npGEX4T-1 GST, AMP R , bacterial expression vector Invitrogen\npAC2058 GST-Nab2-N(aa 1–97) in pGEX4T-1, AMP R This study\npAC2349 GST-Nab2-N F72D(aa 1–97) in pGEX4T-1, AMP R This study\npAC2350 GST-Nab2-N F73D(aa 1–97) in pGEX4T-1, AMP R This study","divisions":[{"label":"tr","span":{"begin":0,"end":45}},{"label":"th","span":{"begin":0,"end":14}},{"label":"th","span":{"begin":16,"end":27}},{"label":"th","span":{"begin":29,"end":45}},{"label":"tr","span":{"begin":46,"end":60}},{"label":"td","span":{"begin":46,"end":60}},{"label":"tr","span":{"begin":60,"end":119}},{"label":"td","span":{"begin":60,"end":72}},{"label":"td","span":{"begin":74,"end":101}},{"label":"td","span":{"begin":103,"end":119}},{"label":"tr","span":{"begin":120,"end":200}},{"label":"td","span":{"begin":120,"end":137}},{"label":"td","span":{"begin":139,"end":183}},{"label":"td","span":{"begin":185,"end":200}},{"label":"tr","span":{"begin":201,"end":261}},{"label":"td","span":{"begin":201,"end":215}},{"label":"td","span":{"begin":217,"end":250}},{"label":"td","span":{"begin":252,"end":261}},{"label":"tr","span":{"begin":262,"end":264}},{"label":"td","span":{"begin":262,"end":264}},{"label":"tr","span":{"begin":265,"end":274}},{"label":"td","span":{"begin":265,"end":274}},{"label":"tr","span":{"begin":274,"end":339}},{"label":"td","span":{"begin":274,"end":282}},{"label":"td","span":{"begin":284,"end":327}},{"label":"td","span":{"begin":329,"end":339}},{"label":"tr","span":{"begin":340,"end":401}},{"label":"td","span":{"begin":340,"end":347}},{"label":"td","span":{"begin":349,"end":389}},{"label":"td","span":{"begin":391,"end":401}},{"label":"tr","span":{"begin":402,"end":468}},{"label":"td","span":{"begin":402,"end":409}},{"label":"td","span":{"begin":411,"end":456}},{"label":"td","span":{"begin":458,"end":468}},{"label":"td","span":{"begin":469,"end":476}},{"label":"td","span":{"begin":478,"end":523}}],"tracks":[]}