Strain/Plasmid Description Source/Reference Yeast strains W303(ACY233) MATαura3Δ leu2Δ trp1Δ his3Δ Winston et al.47 MLP1-TAP (ACY984) MATa his3Δ leu2Δ met15Δ ura3Δ MLP1-TAP::HIS3 Open Biosystems MLP1Δ (ACY481) MATa leu2Δ ura3Δ his3Δ MLP1::HIS3 Euroscarf 

 Plasmids pGEX4T-1 GST, AMP R , bacterial expression vector Invitrogen pAC2058 GST-Nab2-N(aa 1–97) in pGEX4T-1, AMP R This study pAC2349 GST-Nab2-N F72D(aa 1–97) in pGEX4T-1, AMP R This study pAC2350 GST-Nab2-N F73D(aa 1–97) in pGEX4T-1, AMP R This study