PMC:2724026 / 9655-10059 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"The change in stability upon mutation (ΔΔGD-N) was determined using equilibrium denaturation followed by changes in fluorescence. All equilibrium m-values were the same as WT within error, and so ΔΔGD-N was determined using a mean m-value (\u003cm\u003e) of 1.4 ± 0.21 kcal mol- 1 M- 1 as:25(1) ΔΔGD−N=\u003cm\u003e.δ[urea]50%where δ [urea]50% is the difference in the midpoint of denaturation between WT and mutant protein.","divisions":[{"label":"label","span":{"begin":281,"end":284}}],"tracks":[{"project":"2_test","denotations":[{"id":"19362094-2812029-62520519","span":{"begin":279,"end":281},"obj":"2812029"}],"attributes":[{"subj":"19362094-2812029-62520519","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ecc193","default":true}]}]}}