PMC:2724026 / 9655-10059 JSONTXT

{"project":"2_test","denotations":[{"id":"19362094-2812029-62520519","span":{"begin":279,"end":281},"obj":"2812029"}],"text":"The change in stability upon mutation (ΔΔGD-N) was determined using equilibrium denaturation followed by changes in fluorescence. All equilibrium m-values were the same as WT within error, and so ΔΔGD-N was determined using a mean m-value (\u003cm\u003e) of 1.4 ± 0.21 kcal mol- 1 M- 1 as:25(1) ΔΔGD−N=\u003cm\u003e.δ[urea]50%where δ [urea]50% is the difference in the midpoint of denaturation between WT and mutant protein."}