PMC:2222968 / 36227-36655
Annnotations
2_test
{"project":"2_test","denotations":[{"id":"18162042-16387593-84705060","span":{"begin":326,"end":328},"obj":"16387593"},{"id":"T86850","span":{"begin":326,"end":328},"obj":"16387593"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
bionlp-st-ge-2016-spacy-parsed
{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T18366","span":{"begin":0,"end":3},"obj":"DT"},{"id":"T18367","span":{"begin":4,"end":7},"obj":"NNP"},{"id":"T18368","span":{"begin":8,"end":15},"obj":"NNS"},{"id":"T18369","span":{"begin":16,"end":19},"obj":"CC"},{"id":"T18370","span":{"begin":20,"end":26},"obj":"NNS"},{"id":"T18371","span":{"begin":27,"end":31},"obj":"VBD"},{"id":"T18372","span":{"begin":32,"end":40},"obj":"VBN"},{"id":"T18373","span":{"begin":41,"end":46},"obj":"VBN"},{"id":"T18374","span":{"begin":47,"end":49},"obj":"IN"},{"id":"T18375","span":{"begin":50,"end":53},"obj":"DT"},{"id":"T18376","span":{"begin":54,"end":63},"obj":"NNS"},{"id":"T18377","span":{"begin":64,"end":72},"obj":"VBD"},{"id":"T18378","span":{"begin":73,"end":75},"obj":"IN"},{"id":"T18379","span":{"begin":76,"end":83},"obj":"NNP"},{"id":"T18380","span":{"begin":84,"end":88},"obj":"IN"},{"id":"T18381","span":{"begin":89,"end":92},"obj":"DT"},{"id":"T18382","span":{"begin":93,"end":99},"obj":"NNP"},{"id":"T18383","span":{"begin":100,"end":107},"obj":"NNP"},{"id":"T18384","span":{"begin":108,"end":116},"obj":"NN"},{"id":"T18385","span":{"begin":117,"end":124},"obj":"NN"},{"id":"T18386","span":{"begin":125,"end":128},"obj":"CD"},{"id":"T18387","span":{"begin":129,"end":130},"obj":"-LRB-"},{"id":"T18388","span":{"begin":130,"end":137},"obj":"NNP"},{"id":"T18389","span":{"begin":138,"end":148},"obj":"NNPS"},{"id":"T18390","span":{"begin":148,"end":149},"obj":"-RRB-"},{"id":"T18391","span":{"begin":150,"end":152},"obj":"IN"},{"id":"T18392","span":{"begin":153,"end":160},"obj":"VBZ"},{"id":"T18393","span":{"begin":160,"end":161},"obj":":"},{"id":"T18394","span":{"begin":162,"end":167},"obj":"CD"},{"id":"T18395","span":{"begin":168,"end":175},"obj":"RB"},{"id":"T18396","span":{"begin":176,"end":182},"obj":"NN"},{"id":"T18397","span":{"begin":183,"end":184},"obj":"CD"},{"id":"T18398","span":{"begin":184,"end":185},"obj":"SYM"},{"id":"T18399","span":{"begin":185,"end":186},"obj":":"},{"id":"T18400","span":{"begin":186,"end":189},"obj":"NNP"},{"id":"T18401","span":{"begin":190,"end":193},"obj":"NNP"},{"id":"T18402","span":{"begin":194,"end":197},"obj":"NNP"},{"id":"T18403","span":{"begin":198,"end":201},"obj":"NNP"},{"id":"T18404","span":{"begin":202,"end":205},"obj":"NNP"},{"id":"T18405","span":{"begin":206,"end":209},"obj":"NNP"},{"id":"T18406","span":{"begin":210,"end":213},"obj":"NNP"},{"id":"T18407","span":{"begin":214,"end":218},"obj":"JJ"},{"id":"T18408","span":{"begin":218,"end":219},"obj":"NN"},{"id":"T18409","span":{"begin":219,"end":220},"obj":":"},{"id":"T18410","span":{"begin":221,"end":226},"obj":"CD"},{"id":"T18411","span":{"begin":227,"end":234},"obj":"NN"},{"id":"T18412","span":{"begin":235,"end":241},"obj":"NN"},{"id":"T18413","span":{"begin":242,"end":243},"obj":"CD"},{"id":"T18414","span":{"begin":243,"end":244},"obj":"SYM"},{"id":"T18415","span":{"begin":244,"end":245},"obj":":"},{"id":"T18416","span":{"begin":245,"end":248},"obj":"NNP"},{"id":"T18417","span":{"begin":249,"end":252},"obj":"NNP"},{"id":"T18418","span":{"begin":253,"end":256},"obj":"NNP"},{"id":"T18419","span":{"begin":257,"end":260},"obj":"NNP"},{"id":"T18420","span":{"begin":261,"end":264},"obj":"NNP"},{"id":"T18421","span":{"begin":265,"end":269},"obj":"JJ"},{"id":"T18422","span":{"begin":269,"end":270},"obj":"NN"},{"id":"T18423","span":{"begin":270,"end":271},"obj":":"},{"id":"T18424","span":{"begin":272,"end":277},"obj":"NNP"},{"id":"T18425","span":{"begin":278,"end":285},"obj":"RB"},{"id":"T18426","span":{"begin":286,"end":292},"obj":"JJR"},{"id":"T18427","span":{"begin":293,"end":296},"obj":"CC"},{"id":"T18428","span":{"begin":297,"end":304},"obj":"VB"},{"id":"T18429","span":{"begin":305,"end":311},"obj":"NN"},{"id":"T18430","span":{"begin":312,"end":314},"obj":"IN"},{"id":"T18431","span":{"begin":315,"end":324},"obj":"VBN"},{"id":"T18432","span":{"begin":325,"end":326},"obj":"NNP"},{"id":"T18433","span":{"begin":326,"end":328},"obj":"CD"},{"id":"T18434","span":{"begin":328,"end":329},"obj":"NNP"},{"id":"T18435","span":{"begin":329,"end":330},"obj":":"},{"id":"T18436","span":{"begin":331,"end":336},"obj":"NNP"},{"id":"T18437","span":{"begin":337,"end":344},"obj":"RB"},{"id":"T18438","span":{"begin":345,"end":351},"obj":"JJR"},{"id":"T18439","span":{"begin":352,"end":353},"obj":"CD"},{"id":"T18440","span":{"begin":353,"end":354},"obj":"SYM"},{"id":"T18441","span":{"begin":354,"end":355},"obj":":"},{"id":"T18442","span":{"begin":355,"end":358},"obj":"NNP"},{"id":"T18443","span":{"begin":359,"end":362},"obj":"NNP"},{"id":"T18444","span":{"begin":363,"end":366},"obj":"NNP"},{"id":"T18445","span":{"begin":367,"end":370},"obj":"NNP"},{"id":"T18446","span":{"begin":371,"end":374},"obj":"NNP"},{"id":"T18447","span":{"begin":375,"end":378},"obj":"NNP"},{"id":"T18448","span":{"begin":379,"end":384},"obj":"CD"},{"id":"T18449","span":{"begin":384,"end":385},"obj":"NN"},{"id":"T18450","span":{"begin":386,"end":389},"obj":"CC"},{"id":"T18451","span":{"begin":390,"end":393},"obj":"NN"},{"id":"T18452","span":{"begin":394,"end":395},"obj":"CD"},{"id":"T18453","span":{"begin":395,"end":396},"obj":"SYM"},{"id":"T18454","span":{"begin":396,"end":397},"obj":":"},{"id":"T18455","span":{"begin":397,"end":400},"obj":"NNP"},{"id":"T18456","span":{"begin":401,"end":404},"obj":"NNP"},{"id":"T18457","span":{"begin":405,"end":408},"obj":"NNP"},{"id":"T18458","span":{"begin":409,"end":412},"obj":"NNP"},{"id":"T18459","span":{"begin":413,"end":416},"obj":"NNP"},{"id":"T18460","span":{"begin":417,"end":420},"obj":"NNP"},{"id":"T18461","span":{"begin":421,"end":426},"obj":"JJ"},{"id":"T18462","span":{"begin":426,"end":427},"obj":"NN"},{"id":"T18463","span":{"begin":427,"end":428},"obj":"."}],"relations":[{"id":"R13829","pred":"det","subj":"T18366","obj":"T18368"},{"id":"R13830","pred":"compound","subj":"T18367","obj":"T18368"},{"id":"R13831","pred":"nsubjpass","subj":"T18368","obj":"T18372"},{"id":"R13832","pred":"cc","subj":"T18369","obj":"T18368"},{"id":"R13833","pred":"conj","subj":"T18370","obj":"T18368"},{"id":"R13834","pred":"auxpass","subj":"T18371","obj":"T18372"},{"id":"R13835","pred":"ROOT","subj":"T18372","obj":"T18372"},{"id":"R13836","pred":"prep","subj":"T18373","obj":"T18372"},{"id":"R13837","pred":"prep","subj":"T18374","obj":"T18373"},{"id":"R13838","pred":"det","subj":"T18375","obj":"T18376"},{"id":"R13839","pred":"pobj","subj":"T18376","obj":"T18374"},{"id":"R13840","pred":"acl","subj":"T18377","obj":"T18376"},{"id":"R13841","pred":"prep","subj":"T18378","obj":"T18377"},{"id":"R13842","pred":"pobj","subj":"T18379","obj":"T18378"},{"id":"R13843","pred":"prep","subj":"T18380","obj":"T18377"},{"id":"R13844","pred":"det","subj":"T18381","obj":"T18385"},{"id":"R13845","pred":"compound","subj":"T18382","obj":"T18383"},{"id":"R13846","pred":"compound","subj":"T18383","obj":"T18385"},{"id":"R13847","pred":"compound","subj":"T18384","obj":"T18385"},{"id":"R13848","pred":"pobj","subj":"T18385","obj":"T18380"},{"id":"R13849","pred":"nummod","subj":"T18386","obj":"T18385"},{"id":"R13850","pred":"punct","subj":"T18387","obj":"T18389"},{"id":"R13851","pred":"compound","subj":"T18388","obj":"T18389"},{"id":"R13852","pred":"appos","subj":"T18389","obj":"T18385"},{"id":"R13853","pred":"punct","subj":"T18390","obj":"T18377"},{"id":"R13854","pred":"mark","subj":"T18391","obj":"T18392"},{"id":"R13855","pred":"advcl","subj":"T18392","obj":"T18372"},{"id":"R13856","pred":"punct","subj":"T18393","obj":"T18372"},{"id":"R13857","pred":"nummod","subj":"T18394","obj":"T18396"},{"id":"R13858","pred":"amod","subj":"T18395","obj":"T18396"},{"id":"R13859","pred":"ROOT","subj":"T18396","obj":"T18396"},{"id":"R13860","pred":"meta","subj":"T18397","obj":"T18438"},{"id":"R13861","pred":"punct","subj":"T18398","obj":"T18400"},{"id":"R13862","pred":"punct","subj":"T18399","obj":"T18400"},{"id":"R13863","pred":"ccomp","subj":"T18400","obj":"T18438"},{"id":"R13864","pred":"compound","subj":"T18401","obj":"T18402"},{"id":"R13865","pred":"npadvmod","subj":"T18402","obj":"T18426"},{"id":"R13866","pred":"nmod","subj":"T18403","obj":"T18426"},{"id":"R13867","pred":"nmod","subj":"T18404","obj":"T18408"},{"id":"R13868","pred":"nmod","subj":"T18405","obj":"T18408"},{"id":"R13869","pred":"nmod","subj":"T18406","obj":"T18408"},{"id":"R13870","pred":"amod","subj":"T18407","obj":"T18408"},{"id":"R13871","pred":"appos","subj":"T18408","obj":"T18403"},{"id":"R13872","pred":"punct","subj":"T18409","obj":"T18403"},{"id":"R13873","pred":"nummod","subj":"T18410","obj":"T18412"},{"id":"R13874","pred":"compound","subj":"T18411","obj":"T18412"},{"id":"R13875","pred":"appos","subj":"T18412","obj":"T18403"},{"id":"R13876","pred":"nummod","subj":"T18413","obj":"T18426"},{"id":"R13877","pred":"punct","subj":"T18414","obj":"T18426"},{"id":"R13878","pred":"punct","subj":"T18415","obj":"T18426"},{"id":"R13879","pred":"compound","subj":"T18416","obj":"T18417"},{"id":"R13880","pred":"npadvmod","subj":"T18417","obj":"T18426"},{"id":"R13881","pred":"nmod","subj":"T18418","obj":"T18422"},{"id":"R13882","pred":"nmod","subj":"T18419","obj":"T18422"},{"id":"R13883","pred":"nmod","subj":"T18420","obj":"T18422"},{"id":"R13884","pred":"amod","subj":"T18421","obj":"T18422"},{"id":"R13885","pred":"appos","subj":"T18422","obj":"T18417"},{"id":"R13886","pred":"punct","subj":"T18423","obj":"T18417"},{"id":"R13887","pred":"conj","subj":"T18424","obj":"T18417"},{"id":"R13888","pred":"advmod","subj":"T18425","obj":"T18417"},{"id":"R13889","pred":"amod","subj":"T18426","obj":"T18400"},{"id":"R13890","pred":"cc","subj":"T18427","obj":"T18426"},{"id":"R13891","pred":"conj","subj":"T18428","obj":"T18426"},{"id":"R13892","pred":"dobj","subj":"T18429","obj":"T18428"},{"id":"R13893","pred":"mark","subj":"T18430","obj":"T18431"},{"id":"R13894","pred":"advcl","subj":"T18431","obj":"T18438"},{"id":"R13895","pred":"nmod","subj":"T18432","obj":"T18434"},{"id":"R13896","pred":"nummod","subj":"T18433","obj":"T18434"},{"id":"R13897","pred":"dobj","subj":"T18434","obj":"T18431"},{"id":"R13898","pred":"punct","subj":"T18435","obj":"T18438"},{"id":"R13899","pred":"nsubj","subj":"T18436","obj":"T18438"},{"id":"R13900","pred":"advmod","subj":"T18437","obj":"T18438"},{"id":"R13901","pred":"ROOT","subj":"T18438","obj":"T18438"},{"id":"R13902","pred":"nummod","subj":"T18439","obj":"T18462"},{"id":"R13903","pred":"punct","subj":"T18440","obj":"T18444"},{"id":"R13904","pred":"punct","subj":"T18441","obj":"T18444"},{"id":"R13905","pred":"compound","subj":"T18442","obj":"T18444"},{"id":"R13906","pred":"compound","subj":"T18443","obj":"T18444"},{"id":"R13907","pred":"nmod","subj":"T18444","obj":"T18462"},{"id":"R13908","pred":"compound","subj":"T18445","obj":"T18446"},{"id":"R13909","pred":"compound","subj":"T18446","obj":"T18447"},{"id":"R13910","pred":"dep","subj":"T18447","obj":"T18462"},{"id":"R13911","pred":"nummod","subj":"T18448","obj":"T18449"},{"id":"R13912","pred":"nmod","subj":"T18449","obj":"T18462"},{"id":"R13913","pred":"cc","subj":"T18450","obj":"T18449"},{"id":"R13914","pred":"conj","subj":"T18451","obj":"T18449"},{"id":"R13915","pred":"nummod","subj":"T18452","obj":"T18451"},{"id":"R13916","pred":"punct","subj":"T18453","obj":"T18462"},{"id":"R13917","pred":"punct","subj":"T18454","obj":"T18455"},{"id":"R13918","pred":"compound","subj":"T18455","obj":"T18462"},{"id":"R13919","pred":"compound","subj":"T18456","obj":"T18462"},{"id":"R13920","pred":"compound","subj":"T18457","obj":"T18462"},{"id":"R13921","pred":"nmod","subj":"T18458","obj":"T18462"},{"id":"R13922","pred":"compound","subj":"T18459","obj":"T18460"},{"id":"R13923","pred":"nmod","subj":"T18460","obj":"T18462"},{"id":"R13924","pred":"amod","subj":"T18461","obj":"T18462"},{"id":"R13925","pred":"dep","subj":"T18462","obj":"T18438"},{"id":"R13926","pred":"punct","subj":"T18463","obj":"T18438"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T18337","span":{"begin":194,"end":197},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T18338","span":{"begin":413,"end":416},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T18339","span":{"begin":198,"end":201},"obj":"http://purl.obolibrary.org/obo/GO_0004096"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T18701","span":{"begin":194,"end":197},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T18702","span":{"begin":413,"end":416},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T18703","span":{"begin":198,"end":201},"obj":"http://purl.obolibrary.org/obo/GO_0004096"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T18709","span":{"begin":202,"end":205},"obj":"http://purl.obolibrary.org/obo/GO_0005579"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
sentences
{"project":"sentences","denotations":[{"id":"T18329","span":{"begin":0,"end":428},"obj":"Sentence"},{"id":"T269","span":{"begin":0,"end":428},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T18746","span":{"begin":162,"end":167},"obj":"Protein"},{"id":"T18747","span":{"begin":221,"end":226},"obj":"Protein"},{"id":"T18748","span":{"begin":272,"end":277},"obj":"Protein"},{"id":"T18749","span":{"begin":331,"end":336},"obj":"Protein"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T18711","span":{"begin":76,"end":83},"obj":"Protein"},{"id":"T18712","span":{"begin":162,"end":167},"obj":"Protein"},{"id":"T18713","span":{"begin":190,"end":205},"obj":"Protein"},{"id":"T18714","span":{"begin":206,"end":213},"obj":"Protein"},{"id":"T18715","span":{"begin":221,"end":226},"obj":"Protein"},{"id":"T18716","span":{"begin":272,"end":277},"obj":"Protein"},{"id":"T18717","span":{"begin":331,"end":336},"obj":"Protein"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T18320","span":{"begin":162,"end":167},"obj":"Protein"},{"id":"T18321","span":{"begin":221,"end":226},"obj":"Protein"},{"id":"T18322","span":{"begin":272,"end":277},"obj":"Protein"},{"id":"T18323","span":{"begin":331,"end":336},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T18343","span":{"begin":162,"end":167},"obj":"Q9BZS1"},{"id":"T18344","span":{"begin":221,"end":226},"obj":"Q9BZS1"},{"id":"T18345","span":{"begin":331,"end":336},"obj":"P23771"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}
test2
{"project":"test2","denotations":[{"id":"T18312","span":{"begin":162,"end":167},"obj":"Protein"},{"id":"T18313","span":{"begin":221,"end":226},"obj":"Protein"},{"id":"T18314","span":{"begin":272,"end":277},"obj":"Protein"},{"id":"T18315","span":{"begin":331,"end":336},"obj":"Protein"}],"text":"The PCR primers and probes were designed based on the sequences reported in GenBank with the Primer Express software version 1.2 (Applied Biosystems) as follows: FOXP3 forward primer 5′-GAA ACA GCA CAT TCC CAG AGT TC-3′; FOXP3 reverse primer 5′-ATG GCC CAG CGG ATG AG-3′; EF-1α forward primer and reverse primer as described [61]; GATA3 forward primer 5′-GCG GGC TCT ATC ACA AAA TGA-3′ and rwd 5′-GCT CTC CTG GCT GCA GAC AGC-3′."}