Id |
Subject |
Object |
Predicate |
Lexical cue |
T9832 |
0-8 |
NNP |
denotes |
Southern |
T9833 |
9-13 |
NN |
denotes |
blot |
T9834 |
14-23 |
NN |
denotes |
screening |
T9835 |
24-27 |
IN |
denotes |
for |
T9836 |
28-38 |
JJ |
denotes |
homologous |
T9837 |
39-52 |
NN |
denotes |
recombination |
T9838 |
52-140 |
sentence |
denotes |
ES cells genomic DNA was extracted with PUREGENE™ Cell Lysis Solution (Gentra systems). |
T9839 |
53-55 |
NN |
denotes |
ES |
T9840 |
56-61 |
NNS |
denotes |
cells |
T9841 |
70-73 |
NN |
denotes |
DNA |
T9842 |
62-69 |
JJ |
denotes |
genomic |
T9843 |
78-87 |
VBN |
denotes |
extracted |
T9844 |
74-77 |
VBD |
denotes |
was |
T9845 |
88-92 |
IN |
denotes |
with |
T9846 |
93-101 |
NNP |
denotes |
PUREGENE |
T9847 |
114-122 |
NN |
denotes |
Solution |
T9848 |
101-102 |
SYM |
denotes |
™ |
T9849 |
103-107 |
NN |
denotes |
Cell |
T9850 |
108-113 |
NN |
denotes |
Lysis |
T9851 |
123-124 |
-LRB- |
denotes |
( |
T9852 |
131-138 |
NNPS |
denotes |
systems |
T9853 |
124-130 |
NNP |
denotes |
Gentra |
T9854 |
138-139 |
-RRB- |
denotes |
) |
T9855 |
139-140 |
. |
denotes |
. |
T9856 |
140-306 |
sentence |
denotes |
For 5' Southern blot analysis, genomic DNA was first digested with PstI, then separated on an 0.8% agarose gel and transferred to a nylon membrane as described [20]. |
T9857 |
141-144 |
IN |
denotes |
For |
T9858 |
194-202 |
VBN |
denotes |
digested |
T9859 |
145-146 |
CD |
denotes |
5 |
T9860 |
162-170 |
NN |
denotes |
analysis |
T9861 |
146-147 |
SYM |
denotes |
' |
T9862 |
148-156 |
NNP |
denotes |
Southern |
T9863 |
157-161 |
NN |
denotes |
blot |
T9864 |
170-172 |
, |
denotes |
, |
T9865 |
172-179 |
JJ |
denotes |
genomic |
T9866 |
180-183 |
NN |
denotes |
DNA |
T9867 |
184-187 |
VBD |
denotes |
was |
T9868 |
188-193 |
RB |
denotes |
first |
T9869 |
203-207 |
IN |
denotes |
with |
T9870 |
208-212 |
NN |
denotes |
PstI |
T9871 |
212-214 |
, |
denotes |
, |
T9872 |
214-218 |
RB |
denotes |
then |
T9873 |
219-228 |
VBN |
denotes |
separated |
T9874 |
229-231 |
IN |
denotes |
on |
T9875 |
232-234 |
DT |
denotes |
an |
T9876 |
248-251 |
NN |
denotes |
gel |
T9877 |
235-238 |
CD |
denotes |
0.8 |
T9878 |
238-239 |
NN |
denotes |
% |
T9879 |
240-247 |
NN |
denotes |
agarose |
T9880 |
252-255 |
CC |
denotes |
and |
T9881 |
256-267 |
VBN |
denotes |
transferred |
T9882 |
268-270 |
IN |
denotes |
to |
T9883 |
271-272 |
DT |
denotes |
a |
T9884 |
279-287 |
NN |
denotes |
membrane |
T9885 |
273-278 |
NN |
denotes |
nylon |
T9886 |
288-290 |
IN |
denotes |
as |
T9887 |
291-300 |
VBN |
denotes |
described |
T9888 |
301-302 |
-LRB- |
denotes |
[ |
T9889 |
302-304 |
CD |
denotes |
20 |
T9890 |
304-305 |
-RRB- |
denotes |
] |
T9891 |
305-306 |
. |
denotes |
. |
T9892 |
306-443 |
sentence |
denotes |
A 560 bp 5' probe was amplified using the ESG1S5 (5'- GATGGTGGTGGTGACTCAGAG -3') and ESG1AS5 as (5'- CCTCCATTGCCTCTATATCAG -3') primers. |
T9893 |
307-308 |
DT |
denotes |
A |
T9894 |
319-324 |
NN |
denotes |
probe |
T9895 |
309-312 |
CD |
denotes |
560 |
T9896 |
313-315 |
NN |
denotes |
bp |
T9897 |
316-317 |
CD |
denotes |
5 |
T9898 |
317-318 |
SYM |
denotes |
' |
T9899 |
329-338 |
VBN |
denotes |
amplified |
T9900 |
325-328 |
VBD |
denotes |
was |
T9901 |
339-344 |
VBG |
denotes |
using |
T9902 |
345-348 |
DT |
denotes |
the |
T9903 |
349-355 |
NN |
denotes |
ESG1S5 |
T9904 |
356-357 |
-LRB- |
denotes |
( |
T9905 |
361-382 |
NN |
denotes |
GATGGTGGTGGTGACTCAGAG |
T9906 |
357-358 |
CD |
denotes |
5 |
T9907 |
358-359 |
SYM |
denotes |
' |
T9908 |
359-360 |
HYPH |
denotes |
- |
T9909 |
383-384 |
HYPH |
denotes |
- |
T9910 |
384-385 |
CD |
denotes |
3 |
T9911 |
385-386 |
SYM |
denotes |
' |
T9912 |
386-387 |
-RRB- |
denotes |
) |
T9913 |
388-391 |
CC |
denotes |
and |
T9914 |
392-399 |
NN |
denotes |
ESG1AS5 |
T9915 |
400-402 |
IN |
denotes |
as |
T9916 |
403-404 |
-LRB- |
denotes |
( |
T9917 |
435-442 |
NNS |
denotes |
primers |
T9918 |
404-405 |
CD |
denotes |
5 |
T9919 |
408-429 |
NN |
denotes |
CCTCCATTGCCTCTATATCAG |
T9920 |
405-406 |
SYM |
denotes |
' |
T9921 |
406-407 |
HYPH |
denotes |
- |
T9922 |
430-431 |
HYPH |
denotes |
- |
T9923 |
431-432 |
CD |
denotes |
3 |
T9924 |
432-433 |
SYM |
denotes |
' |
T9925 |
433-434 |
-RRB- |
denotes |
) |
T9926 |
442-443 |
. |
denotes |
. |
T9927 |
443-590 |
sentence |
denotes |
The probe specifically labeled an 18 kbp band from the wild-type locus, a 15 kbp band from the β-geo locus, and a 12 kbp band from the HygR locus. |
T9928 |
444-447 |
DT |
denotes |
The |
T9929 |
448-453 |
NN |
denotes |
probe |
T9930 |
467-474 |
VBD |
denotes |
labeled |
T9931 |
454-466 |
RB |
denotes |
specifically |
T9932 |
475-477 |
DT |
denotes |
an |
T9933 |
485-489 |
NN |
denotes |
band |
T9934 |
478-480 |
CD |
denotes |
18 |
T9935 |
481-484 |
NN |
denotes |
kbp |
T9936 |
490-494 |
IN |
denotes |
from |
T9937 |
495-498 |
DT |
denotes |
the |
T9938 |
509-514 |
NN |
denotes |
locus |
T9939 |
499-503 |
JJ |
denotes |
wild |
T9940 |
503-504 |
HYPH |
denotes |
- |
T9941 |
504-508 |
NN |
denotes |
type |
T9942 |
514-516 |
, |
denotes |
, |
T9943 |
516-517 |
DT |
denotes |
a |
T9944 |
525-529 |
NN |
denotes |
band |
T9945 |
518-520 |
CD |
denotes |
15 |
T9946 |
521-524 |
NN |
denotes |
kbp |
T9947 |
530-534 |
IN |
denotes |
from |
T9948 |
535-538 |
DT |
denotes |
the |
T9949 |
545-550 |
NN |
denotes |
locus |
T9950 |
539-540 |
NN |
denotes |
β |
T9951 |
541-544 |
NN |
denotes |
geo |
T9952 |
540-541 |
HYPH |
denotes |
- |
T9953 |
550-552 |
, |
denotes |
, |
T9954 |
552-555 |
CC |
denotes |
and |
T9955 |
556-557 |
DT |
denotes |
a |
T9956 |
565-569 |
NN |
denotes |
band |
T9957 |
558-560 |
CD |
denotes |
12 |
T9958 |
561-564 |
NN |
denotes |
kbp |
T9959 |
570-574 |
IN |
denotes |
from |
T9960 |
575-578 |
DT |
denotes |
the |
T9961 |
584-589 |
NN |
denotes |
locus |
T9962 |
579-583 |
NN |
denotes |
HygR |
T9963 |
589-590 |
. |
denotes |
. |
T9964 |
590-661 |
sentence |
denotes |
Genomic DNA was also digested with SpeI for 3' Southern blot analysis. |
T9965 |
591-598 |
JJ |
denotes |
Genomic |
T9966 |
599-602 |
NN |
denotes |
DNA |
T9967 |
612-620 |
VBN |
denotes |
digested |
T9968 |
603-606 |
VBD |
denotes |
was |
T9969 |
607-611 |
RB |
denotes |
also |
T9970 |
621-625 |
IN |
denotes |
with |
T9971 |
626-630 |
NN |
denotes |
SpeI |
T9972 |
631-634 |
IN |
denotes |
for |
T9973 |
635-636 |
CD |
denotes |
3 |
T9974 |
652-660 |
NN |
denotes |
analysis |
T9975 |
636-637 |
SYM |
denotes |
' |
T9976 |
638-646 |
NNP |
denotes |
Southern |
T9977 |
647-651 |
NN |
denotes |
blot |
T9978 |
660-661 |
. |
denotes |
. |
T9979 |
661-808 |
sentence |
denotes |
A 1,010 bp 3' probe was amplified with the pH34U-8000 (5'- CCAACCAGCCAGAGTTTCAGTTAT -3') and pH34L-9000 (5'-GATAAGCTGCTGCCAAAAGACAAG -3') primers. |
T9980 |
662-663 |
DT |
denotes |
A |
T9981 |
676-681 |
NN |
denotes |
probe |
T9982 |
664-669 |
CD |
denotes |
1,010 |
T9983 |
670-672 |
NN |
denotes |
bp |
T9984 |
673-674 |
CD |
denotes |
3 |
T9985 |
674-675 |
SYM |
denotes |
' |
T9986 |
686-695 |
VBN |
denotes |
amplified |
T9987 |
682-685 |
VBD |
denotes |
was |
T9988 |
696-700 |
IN |
denotes |
with |
T9989 |
701-704 |
DT |
denotes |
the |
T9990 |
800-807 |
NNS |
denotes |
primers |
T9991 |
705-710 |
NN |
denotes |
pH34U |
T9992 |
710-711 |
HYPH |
denotes |
- |
T9993 |
711-715 |
CD |
denotes |
8000 |
T9994 |
716-717 |
-LRB- |
denotes |
( |
T9995 |
721-745 |
NN |
denotes |
CCAACCAGCCAGAGTTTCAGTTAT |
T9996 |
717-718 |
CD |
denotes |
5 |
T9997 |
718-719 |
SYM |
denotes |
' |
T9998 |
719-720 |
HYPH |
denotes |
- |
T9999 |
746-747 |
HYPH |
denotes |
- |
T10000 |
747-748 |
CD |
denotes |
3 |
T10001 |
748-749 |
SYM |
denotes |
' |
T10002 |
749-750 |
-RRB- |
denotes |
) |
T10003 |
751-754 |
CC |
denotes |
and |
T10004 |
755-760 |
NN |
denotes |
pH34L |
T10005 |
760-761 |
HYPH |
denotes |
- |
T10006 |
761-765 |
CD |
denotes |
9000 |
T10007 |
766-767 |
-LRB- |
denotes |
( |
T10008 |
770-794 |
NN |
denotes |
GATAAGCTGCTGCCAAAAGACAAG |
T10009 |
767-768 |
CD |
denotes |
5 |
T10010 |
768-769 |
SYM |
denotes |
' |
T10011 |
769-770 |
HYPH |
denotes |
- |
T10012 |
795-796 |
HYPH |
denotes |
- |
T10013 |
796-797 |
CD |
denotes |
3 |
T10014 |
797-798 |
SYM |
denotes |
' |
T10015 |
798-799 |
-RRB- |
denotes |
) |
T10016 |
807-808 |
. |
denotes |
. |
T10017 |
808-953 |
sentence |
denotes |
The probe hybridized to an 11.5 kbp band from the wild-type locus, a 12.5 kbp band from the β-geo locus, and a 9.5 kbp band from the HygR locus. |
T10018 |
809-812 |
DT |
denotes |
The |
T10019 |
813-818 |
NN |
denotes |
probe |
T10020 |
819-829 |
VBD |
denotes |
hybridized |
T10021 |
830-832 |
IN |
denotes |
to |
T10022 |
833-835 |
DT |
denotes |
an |
T10023 |
845-849 |
NN |
denotes |
band |
T10024 |
836-840 |
CD |
denotes |
11.5 |
T10025 |
841-844 |
NN |
denotes |
kbp |
T10026 |
850-854 |
IN |
denotes |
from |
T10027 |
855-858 |
DT |
denotes |
the |
T10028 |
869-874 |
NN |
denotes |
locus |
T10029 |
859-863 |
JJ |
denotes |
wild |
T10030 |
864-868 |
NN |
denotes |
type |
T10031 |
863-864 |
HYPH |
denotes |
- |
T10032 |
874-876 |
, |
denotes |
, |
T10033 |
876-877 |
DT |
denotes |
a |
T10034 |
887-891 |
NN |
denotes |
band |
T10035 |
878-882 |
CD |
denotes |
12.5 |
T10036 |
883-886 |
NN |
denotes |
kbp |
T10037 |
892-896 |
IN |
denotes |
from |
T10038 |
897-900 |
DT |
denotes |
the |
T10039 |
907-912 |
NN |
denotes |
locus |
T10040 |
901-902 |
NN |
denotes |
β |
T10041 |
903-906 |
NN |
denotes |
geo |
T10042 |
902-903 |
HYPH |
denotes |
- |
T10043 |
912-914 |
, |
denotes |
, |
T10044 |
914-917 |
CC |
denotes |
and |
T10045 |
918-919 |
DT |
denotes |
a |
T10046 |
928-932 |
NN |
denotes |
band |
T10047 |
920-923 |
CD |
denotes |
9.5 |
T10048 |
924-927 |
NN |
denotes |
kbp |
T10049 |
933-937 |
IN |
denotes |
from |
T10050 |
938-941 |
DT |
denotes |
the |
T10051 |
947-952 |
NN |
denotes |
locus |
T10052 |
942-946 |
NN |
denotes |
HygR |
T10053 |
952-953 |
. |
denotes |
. |
R2683 |
T9832 |
T9833 |
compound |
Southern,blot |
R2684 |
T9833 |
T9834 |
compound |
blot,screening |
R2685 |
T9835 |
T9834 |
prep |
for,screening |
R2686 |
T9836 |
T9837 |
amod |
homologous,recombination |
R2687 |
T9837 |
T9835 |
pobj |
recombination,for |
R2688 |
T9839 |
T9840 |
nmod |
ES,cells |
R2689 |
T9840 |
T9841 |
nmod |
cells,DNA |
R2690 |
T9841 |
T9843 |
nsubjpass |
DNA,extracted |
R2691 |
T9842 |
T9841 |
amod |
genomic,DNA |
R2692 |
T9844 |
T9843 |
auxpass |
was,extracted |
R2693 |
T9845 |
T9843 |
prep |
with,extracted |
R2694 |
T9846 |
T9847 |
nmod |
PUREGENE,Solution |
R2695 |
T9847 |
T9845 |
pobj |
Solution,with |
R2696 |
T9848 |
T9846 |
punct |
™,PUREGENE |
R2697 |
T9849 |
T9850 |
compound |
Cell,Lysis |
R2698 |
T9850 |
T9847 |
compound |
Lysis,Solution |
R2699 |
T9851 |
T9852 |
punct |
(,systems |
R2700 |
T9852 |
T9847 |
parataxis |
systems,Solution |
R2701 |
T9853 |
T9852 |
compound |
Gentra,systems |
R2702 |
T9854 |
T9852 |
punct |
),systems |
R2703 |
T9855 |
T9843 |
punct |
.,extracted |
R2704 |
T9857 |
T9858 |
prep |
For,digested |
R2705 |
T9859 |
T9860 |
nummod |
5,analysis |
R2706 |
T9860 |
T9857 |
pobj |
analysis,For |
R2707 |
T9861 |
T9859 |
punct |
',5 |
R2708 |
T9862 |
T9863 |
compound |
Southern,blot |
R2709 |
T9863 |
T9860 |
compound |
blot,analysis |
R2710 |
T9864 |
T9858 |
punct |
", ",digested |
R2711 |
T9865 |
T9866 |
amod |
genomic,DNA |
R2712 |
T9866 |
T9858 |
nsubjpass |
DNA,digested |
R2713 |
T9867 |
T9858 |
auxpass |
was,digested |
R2714 |
T9868 |
T9858 |
advmod |
first,digested |
R2715 |
T9869 |
T9858 |
prep |
with,digested |
R2716 |
T9870 |
T9869 |
pobj |
PstI,with |
R2717 |
T9871 |
T9858 |
punct |
", ",digested |
R2718 |
T9872 |
T9873 |
advmod |
then,separated |
R2719 |
T9873 |
T9858 |
conj |
separated,digested |
R2720 |
T9874 |
T9873 |
prep |
on,separated |
R2721 |
T9875 |
T9876 |
det |
an,gel |
R2722 |
T9876 |
T9874 |
pobj |
gel,on |
R2723 |
T9877 |
T9878 |
nummod |
0.8,% |
R2724 |
T9878 |
T9876 |
compound |
%,gel |
R2725 |
T9879 |
T9876 |
compound |
agarose,gel |
R2726 |
T9880 |
T9873 |
cc |
and,separated |
R2727 |
T9881 |
T9873 |
conj |
transferred,separated |
R2728 |
T9882 |
T9881 |
prep |
to,transferred |
R2729 |
T9883 |
T9884 |
det |
a,membrane |
R2730 |
T9884 |
T9882 |
pobj |
membrane,to |
R2731 |
T9885 |
T9884 |
compound |
nylon,membrane |
R2732 |
T9886 |
T9887 |
mark |
as,described |
R2733 |
T9887 |
T9881 |
advcl |
described,transferred |
R2734 |
T9888 |
T9889 |
punct |
[,20 |
R2735 |
T9889 |
T9887 |
parataxis |
20,described |
R2736 |
T9890 |
T9889 |
punct |
],20 |
R2737 |
T9891 |
T9858 |
punct |
.,digested |
R2738 |
T9893 |
T9894 |
det |
A,probe |
R2739 |
T9894 |
T9899 |
nsubjpass |
probe,amplified |
R2740 |
T9895 |
T9896 |
nummod |
560,bp |
R2741 |
T9896 |
T9894 |
nmod |
bp,probe |
R2742 |
T9897 |
T9894 |
nummod |
5,probe |
R2743 |
T9898 |
T9897 |
punct |
',5 |
R2744 |
T9900 |
T9899 |
auxpass |
was,amplified |
R2745 |
T9901 |
T9899 |
advcl |
using,amplified |
R2746 |
T9902 |
T9903 |
det |
the,ESG1S5 |
R2747 |
T9903 |
T9901 |
dobj |
ESG1S5,using |
R2748 |
T9904 |
T9905 |
punct |
(,GATGGTGGTGGTGACTCAGAG |
R2749 |
T9905 |
T9903 |
parataxis |
GATGGTGGTGGTGACTCAGAG,ESG1S5 |
R2750 |
T9906 |
T9905 |
nummod |
5,GATGGTGGTGGTGACTCAGAG |
R2751 |
T9907 |
T9906 |
punct |
',5 |
R2752 |
T9908 |
T9905 |
punct |
-,GATGGTGGTGGTGACTCAGAG |
R2753 |
T9909 |
T9905 |
punct |
-,GATGGTGGTGGTGACTCAGAG |
R2754 |
T9910 |
T9905 |
nummod |
3,GATGGTGGTGGTGACTCAGAG |
R2755 |
T9911 |
T9905 |
punct |
',GATGGTGGTGGTGACTCAGAG |
R2756 |
T9912 |
T9905 |
punct |
),GATGGTGGTGGTGACTCAGAG |
R2757 |
T9913 |
T9903 |
cc |
and,ESG1S5 |
R2758 |
T9914 |
T9903 |
conj |
ESG1AS5,ESG1S5 |
R2759 |
T9915 |
T9901 |
prep |
as,using |
R2760 |
T9916 |
T9917 |
punct |
(,primers |
R2761 |
T9917 |
T9915 |
pobj |
primers,as |
R2762 |
T9918 |
T9919 |
nummod |
5,CCTCCATTGCCTCTATATCAG |
R2763 |
T9919 |
T9917 |
nmod |
CCTCCATTGCCTCTATATCAG,primers |
R2764 |
T9920 |
T9918 |
punct |
',5 |
R2765 |
T9921 |
T9919 |
punct |
-,CCTCCATTGCCTCTATATCAG |
R2766 |
T9922 |
T9919 |
punct |
-,CCTCCATTGCCTCTATATCAG |
R2767 |
T9923 |
T9919 |
nummod |
3,CCTCCATTGCCTCTATATCAG |
R2768 |
T9924 |
T9919 |
punct |
',CCTCCATTGCCTCTATATCAG |
R2769 |
T9925 |
T9919 |
punct |
),CCTCCATTGCCTCTATATCAG |
R2770 |
T9926 |
T9899 |
punct |
.,amplified |
R2771 |
T9928 |
T9929 |
det |
The,probe |
R2772 |
T9929 |
T9930 |
nsubj |
probe,labeled |
R2773 |
T9931 |
T9930 |
advmod |
specifically,labeled |
R2774 |
T9932 |
T9933 |
det |
an,band |
R2775 |
T9933 |
T9930 |
dobj |
band,labeled |
R2776 |
T9934 |
T9935 |
nummod |
18,kbp |
R2777 |
T9935 |
T9933 |
compound |
kbp,band |
R2778 |
T9936 |
T9933 |
prep |
from,band |
R2779 |
T9937 |
T9938 |
det |
the,locus |
R2780 |
T9938 |
T9936 |
pobj |
locus,from |
R2781 |
T9939 |
T9938 |
nmod |
wild,locus |
R2782 |
T9940 |
T9939 |
punct |
-,wild |
R2783 |
T9941 |
T9938 |
compound |
type,locus |
R2784 |
T9942 |
T9933 |
punct |
", ",band |
R2785 |
T9943 |
T9944 |
det |
a,band |
R2786 |
T9944 |
T9933 |
conj |
band,band |
R2787 |
T9945 |
T9946 |
nummod |
15,kbp |
R2788 |
T9946 |
T9944 |
compound |
kbp,band |
R2789 |
T9947 |
T9944 |
prep |
from,band |
R2790 |
T9948 |
T9949 |
det |
the,locus |
R2791 |
T9949 |
T9947 |
pobj |
locus,from |
R2792 |
T9950 |
T9951 |
compound |
β,geo |
R2793 |
T9951 |
T9949 |
compound |
geo,locus |
R2794 |
T9952 |
T9951 |
punct |
-,geo |
R2795 |
T9953 |
T9944 |
punct |
", ",band |
R2796 |
T9954 |
T9944 |
cc |
and,band |
R2797 |
T9955 |
T9956 |
det |
a,band |
R2798 |
T9956 |
T9944 |
conj |
band,band |
R2799 |
T9957 |
T9958 |
nummod |
12,kbp |
R2800 |
T9958 |
T9956 |
compound |
kbp,band |
R2801 |
T9959 |
T9956 |
prep |
from,band |
R2802 |
T9960 |
T9961 |
det |
the,locus |
R2803 |
T9961 |
T9959 |
pobj |
locus,from |
R2804 |
T9962 |
T9961 |
compound |
HygR,locus |
R2805 |
T9963 |
T9930 |
punct |
.,labeled |
R2806 |
T9965 |
T9966 |
amod |
Genomic,DNA |
R2807 |
T9966 |
T9967 |
nsubjpass |
DNA,digested |
R2808 |
T9968 |
T9967 |
auxpass |
was,digested |
R2809 |
T9969 |
T9967 |
advmod |
also,digested |
R2810 |
T9970 |
T9967 |
prep |
with,digested |
R2811 |
T9971 |
T9970 |
pobj |
SpeI,with |
R2812 |
T9972 |
T9967 |
prep |
for,digested |
R2813 |
T9973 |
T9974 |
nummod |
3,analysis |
R2814 |
T9974 |
T9972 |
pobj |
analysis,for |
R2815 |
T9975 |
T9973 |
punct |
',3 |
R2816 |
T9976 |
T9977 |
compound |
Southern,blot |
R2817 |
T9977 |
T9974 |
compound |
blot,analysis |
R2818 |
T9978 |
T9967 |
punct |
.,digested |
R2819 |
T9980 |
T9981 |
det |
A,probe |
R2820 |
T9981 |
T9986 |
nsubjpass |
probe,amplified |
R2821 |
T9982 |
T9983 |
nummod |
"1,010",bp |
R2822 |
T9983 |
T9981 |
nmod |
bp,probe |
R2823 |
T9984 |
T9981 |
nummod |
3,probe |
R2824 |
T9985 |
T9984 |
punct |
',3 |
R2825 |
T9987 |
T9986 |
auxpass |
was,amplified |
R2826 |
T9988 |
T9986 |
prep |
with,amplified |
R2827 |
T9989 |
T9990 |
det |
the,primers |
R2828 |
T9990 |
T9988 |
pobj |
primers,with |
R2829 |
T9991 |
T9990 |
nmod |
pH34U,primers |
R2830 |
T9992 |
T9991 |
punct |
-,pH34U |
R2831 |
T9993 |
T9991 |
nummod |
8000,pH34U |
R2832 |
T9994 |
T9995 |
punct |
(,CCAACCAGCCAGAGTTTCAGTTAT |
R2833 |
T9995 |
T9991 |
parataxis |
CCAACCAGCCAGAGTTTCAGTTAT,pH34U |
R2834 |
T9996 |
T9995 |
nummod |
5,CCAACCAGCCAGAGTTTCAGTTAT |
R2835 |
T9997 |
T9996 |
punct |
',5 |
R2836 |
T9998 |
T9995 |
punct |
-,CCAACCAGCCAGAGTTTCAGTTAT |
R2837 |
T9999 |
T9995 |
punct |
-,CCAACCAGCCAGAGTTTCAGTTAT |
R2838 |
T10000 |
T9995 |
nummod |
3,CCAACCAGCCAGAGTTTCAGTTAT |
R2839 |
T10001 |
T9995 |
punct |
',CCAACCAGCCAGAGTTTCAGTTAT |
R2840 |
T10002 |
T9995 |
punct |
),CCAACCAGCCAGAGTTTCAGTTAT |
R2841 |
T10003 |
T9991 |
cc |
and,pH34U |
R2842 |
T10004 |
T9991 |
conj |
pH34L,pH34U |
R2843 |
T10005 |
T10004 |
punct |
-,pH34L |
R2844 |
T10006 |
T10004 |
nummod |
9000,pH34L |
R2845 |
T10007 |
T10008 |
punct |
(,GATAAGCTGCTGCCAAAAGACAAG |
R2846 |
T10008 |
T10004 |
parataxis |
GATAAGCTGCTGCCAAAAGACAAG,pH34L |
R2847 |
T10009 |
T10008 |
nummod |
5,GATAAGCTGCTGCCAAAAGACAAG |
R2848 |
T10010 |
T10009 |
punct |
',5 |
R2849 |
T10011 |
T10008 |
punct |
-,GATAAGCTGCTGCCAAAAGACAAG |
R2850 |
T10012 |
T10008 |
punct |
-,GATAAGCTGCTGCCAAAAGACAAG |
R2851 |
T10013 |
T10008 |
nummod |
3,GATAAGCTGCTGCCAAAAGACAAG |
R2852 |
T10014 |
T10008 |
punct |
',GATAAGCTGCTGCCAAAAGACAAG |
R2853 |
T10015 |
T10008 |
punct |
),GATAAGCTGCTGCCAAAAGACAAG |
R2854 |
T10016 |
T9986 |
punct |
.,amplified |
R2855 |
T10018 |
T10019 |
det |
The,probe |
R2856 |
T10019 |
T10020 |
nsubj |
probe,hybridized |
R2857 |
T10021 |
T10020 |
prep |
to,hybridized |
R2858 |
T10022 |
T10023 |
det |
an,band |
R2859 |
T10023 |
T10021 |
pobj |
band,to |
R2860 |
T10024 |
T10025 |
nummod |
11.5,kbp |
R2861 |
T10025 |
T10023 |
compound |
kbp,band |
R2862 |
T10026 |
T10023 |
prep |
from,band |
R2863 |
T10027 |
T10028 |
det |
the,locus |
R2864 |
T10028 |
T10026 |
pobj |
locus,from |
R2865 |
T10029 |
T10030 |
amod |
wild,type |
R2866 |
T10030 |
T10028 |
compound |
type,locus |
R2867 |
T10031 |
T10030 |
punct |
-,type |
R2868 |
T10032 |
T10023 |
punct |
", ",band |
R2869 |
T10033 |
T10034 |
det |
a,band |
R2870 |
T10034 |
T10023 |
conj |
band,band |
R2871 |
T10035 |
T10036 |
nummod |
12.5,kbp |
R2872 |
T10036 |
T10034 |
compound |
kbp,band |
R2873 |
T10037 |
T10034 |
prep |
from,band |
R2874 |
T10038 |
T10039 |
det |
the,locus |
R2875 |
T10039 |
T10037 |
pobj |
locus,from |
R2876 |
T10040 |
T10041 |
compound |
β,geo |
R2877 |
T10041 |
T10039 |
compound |
geo,locus |
R2878 |
T10042 |
T10041 |
punct |
-,geo |
R2879 |
T10043 |
T10034 |
punct |
", ",band |
R2880 |
T10044 |
T10034 |
cc |
and,band |
R2881 |
T10045 |
T10046 |
det |
a,band |
R2882 |
T10046 |
T10034 |
conj |
band,band |
R2883 |
T10047 |
T10048 |
nummod |
9.5,kbp |
R2884 |
T10048 |
T10046 |
compound |
kbp,band |
R2885 |
T10049 |
T10046 |
prep |
from,band |
R2886 |
T10050 |
T10051 |
det |
the,locus |
R2887 |
T10051 |
T10049 |
pobj |
locus,from |
R2888 |
T10052 |
T10051 |
compound |
HygR,locus |
R2889 |
T10053 |
T10020 |
punct |
.,hybridized |