PMC:1382200 / 881-1254 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/1382200","sourcedb":"PMC","sourceid":"1382200","source_url":"http://www.ncbi.nlm.nih.gov/pmc/1382200","text":"ween the alternatives described above, we deleted an essential coding region in the mouse Capn2 gene in embryonic stems cells and transmitted this mutant allele through the mouse germline. Breeding of heterozygous animals failed to produce homozygous mutant live offspring or implanted embryos. A nested PCR genotyping protocol was established, and homozygous preimplantati","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T833","span":{"begin":42,"end":49},"obj":"VBD"},{"id":"T835","span":{"begin":5,"end":8},"obj":"DT"},{"id":"T836","span":{"begin":9,"end":21},"obj":"NNS"},{"id":"T837","span":{"begin":22,"end":31},"obj":"VBN"},{"id":"T838","span":{"begin":32,"end":37},"obj":"RB"},{"id":"T839","span":{"begin":37,"end":39},"obj":","},{"id":"T840","span":{"begin":39,"end":41},"obj":"PRP"},{"id":"T841","span":{"begin":50,"end":52},"obj":"DT"},{"id":"T842","span":{"begin":70,"end":76},"obj":"NN"},{"id":"T843","span":{"begin":53,"end":62},"obj":"JJ"},{"id":"T844","span":{"begin":63,"end":69},"obj":"NN"},{"id":"T845","span":{"begin":77,"end":79},"obj":"IN"},{"id":"T846","span":{"begin":80,"end":83},"obj":"DT"},{"id":"T847","span":{"begin":96,"end":100},"obj":"NN"},{"id":"T848","span":{"begin":84,"end":89},"obj":"NN"},{"id":"T849","span":{"begin":90,"end":95},"obj":"NN"},{"id":"T850","span":{"begin":101,"end":103},"obj":"IN"},{"id":"T851","span":{"begin":104,"end":113},"obj":"JJ"},{"id":"T852","span":{"begin":120,"end":125},"obj":"NNS"},{"id":"T853","span":{"begin":114,"end":119},"obj":"VBZ"},{"id":"T854","span":{"begin":126,"end":129},"obj":"CC"},{"id":"T855","span":{"begin":130,"end":141},"obj":"VBN"},{"id":"T856","span":{"begin":142,"end":146},"obj":"DT"},{"id":"T857","span":{"begin":154,"end":160},"obj":"NN"},{"id":"T858","span":{"begin":147,"end":153},"obj":"NN"},{"id":"T859","span":{"begin":161,"end":168},"obj":"IN"},{"id":"T860","span":{"begin":169,"end":172},"obj":"DT"},{"id":"T861","span":{"begin":179,"end":187},"obj":"NN"},{"id":"T862","span":{"begin":173,"end":178},"obj":"NN"},{"id":"T863","span":{"begin":187,"end":188},"obj":"."},{"id":"T864","span":{"begin":188,"end":294},"obj":"sentence"},{"id":"T865","span":{"begin":189,"end":197},"obj":"NN"},{"id":"T866","span":{"begin":222,"end":228},"obj":"VBD"},{"id":"T867","span":{"begin":198,"end":200},"obj":"IN"},{"id":"T868","span":{"begin":201,"end":213},"obj":"JJ"},{"id":"T869","span":{"begin":214,"end":221},"obj":"NNS"},{"id":"T870","span":{"begin":229,"end":231},"obj":"TO"},{"id":"T871","span":{"begin":232,"end":239},"obj":"VB"},{"id":"T872","span":{"begin":240,"end":250},"obj":"JJ"},{"id":"T873","span":{"begin":263,"end":272},"obj":"NN"},{"id":"T874","span":{"begin":251,"end":257},"obj":"NN"},{"id":"T875","span":{"begin":258,"end":262},"obj":"JJ"},{"id":"T876","span":{"begin":273,"end":275},"obj":"CC"},{"id":"T877","span":{"begin":276,"end":285},"obj":"VBN"},{"id":"T878","span":{"begin":286,"end":293},"obj":"NNS"},{"id":"T879","span":{"begin":293,"end":294},"obj":"."},{"id":"T881","span":{"begin":295,"end":296},"obj":"DT"},{"id":"T882","span":{"begin":319,"end":327},"obj":"NN"},{"id":"T883","span":{"begin":297,"end":303},"obj":"VBN"},{"id":"T884","span":{"begin":304,"end":307},"obj":"NN"},{"id":"T885","span":{"begin":308,"end":318},"obj":"NN"},{"id":"T886","span":{"begin":332,"end":343},"obj":"VBN"},{"id":"T887","span":{"begin":328,"end":331},"obj":"VBD"},{"id":"T888","span":{"begin":343,"end":345},"obj":","},{"id":"T889","span":{"begin":345,"end":348},"obj":"CC"},{"id":"T890","span":{"begin":349,"end":359},"obj":"JJ"}],"relations":[{"id":"R155","pred":"det","subj":"T835","obj":"T836"},{"id":"R157","pred":"acl","subj":"T837","obj":"T836"},{"id":"R158","pred":"advmod","subj":"T838","obj":"T837"},{"id":"R159","pred":"punct","subj":"T839","obj":"T833"},{"id":"R160","pred":"nsubj","subj":"T840","obj":"T833"},{"id":"R161","pred":"det","subj":"T841","obj":"T842"},{"id":"R162","pred":"dobj","subj":"T842","obj":"T833"},{"id":"R163","pred":"amod","subj":"T843","obj":"T842"},{"id":"R164","pred":"compound","subj":"T844","obj":"T842"},{"id":"R165","pred":"prep","subj":"T845","obj":"T842"},{"id":"R166","pred":"det","subj":"T846","obj":"T847"},{"id":"R167","pred":"pobj","subj":"T847","obj":"T845"},{"id":"R168","pred":"compound","subj":"T848","obj":"T847"},{"id":"R169","pred":"compound","subj":"T849","obj":"T847"},{"id":"R170","pred":"prep","subj":"T850","obj":"T833"},{"id":"R171","pred":"amod","subj":"T851","obj":"T852"},{"id":"R173","pred":"nmod","subj":"T853","obj":"T852"},{"id":"R174","pred":"cc","subj":"T854","obj":"T833"},{"id":"R175","pred":"conj","subj":"T855","obj":"T833"},{"id":"R176","pred":"det","subj":"T856","obj":"T857"},{"id":"R178","pred":"compound","subj":"T858","obj":"T857"},{"id":"R179","pred":"prep","subj":"T859","obj":"T855"},{"id":"R180","pred":"det","subj":"T860","obj":"T861"},{"id":"R182","pred":"compound","subj":"T862","obj":"T861"},{"id":"R183","pred":"punct","subj":"T863","obj":"T833"},{"id":"R184","pred":"nsubj","subj":"T865","obj":"T866"},{"id":"R185","pred":"prep","subj":"T867","obj":"T865"},{"id":"R186","pred":"amod","subj":"T868","obj":"T869"},{"id":"R187","pred":"pobj","subj":"T869","obj":"T867"},{"id":"R188","pred":"aux","subj":"T870","obj":"T871"},{"id":"R189","pred":"xcomp","subj":"T871","obj":"T866"},{"id":"R190","pred":"amod","subj":"T872","obj":"T873"},{"id":"R191","pred":"dobj","subj":"T873","obj":"T871"},{"id":"R192","pred":"nmod","subj":"T874","obj":"T873"},{"id":"R193","pred":"amod","subj":"T875","obj":"T873"},{"id":"R194","pred":"cc","subj":"T876","obj":"T873"},{"id":"R195","pred":"amod","subj":"T877","obj":"T878"},{"id":"R196","pred":"conj","subj":"T878","obj":"T873"},{"id":"R197","pred":"punct","subj":"T879","obj":"T866"},{"id":"R198","pred":"det","subj":"T881","obj":"T882"},{"id":"R199","pred":"nsubjpass","subj":"T882","obj":"T886"},{"id":"R200","pred":"amod","subj":"T883","obj":"T882"},{"id":"R201","pred":"compound","subj":"T884","obj":"T882"},{"id":"R202","pred":"compound","subj":"T885","obj":"T882"},{"id":"R203","pred":"auxpass","subj":"T887","obj":"T886"},{"id":"R204","pred":"punct","subj":"T888","obj":"T886"},{"id":"R205","pred":"cc","subj":"T889","obj":"T886"},{"id":"R172","pred":"pobj","subj":"T852","obj":"T850"},{"id":"R177","pred":"dobj","subj":"T857","obj":"T855"},{"id":"R181","pred":"pobj","subj":"T861","obj":"T859"}],"attributes":[{"subj":"T833","pred":"source","obj":"craft-sa-dev"},{"subj":"T835","pred":"source","obj":"craft-sa-dev"},{"subj":"T836","pred":"source","obj":"craft-sa-dev"},{"subj":"T837","pred":"source","obj":"craft-sa-dev"},{"subj":"T838","pred":"source","obj":"craft-sa-dev"},{"subj":"T839","pred":"source","obj":"craft-sa-dev"},{"subj":"T840","pred":"source","obj":"craft-sa-dev"},{"subj":"T841","pred":"source","obj":"craft-sa-dev"},{"subj":"T842","pred":"source","obj":"craft-sa-dev"},{"subj":"T843","pred":"source","obj":"craft-sa-dev"},{"subj":"T844","pred":"source","obj":"craft-sa-dev"},{"subj":"T845","pred":"source","obj":"craft-sa-dev"},{"subj":"T846","pred":"source","obj":"craft-sa-dev"},{"subj":"T847","pred":"source","obj":"craft-sa-dev"},{"subj":"T848","pred":"source","obj":"craft-sa-dev"},{"subj":"T849","pred":"source","obj":"craft-sa-dev"},{"subj":"T850","pred":"source","obj":"craft-sa-dev"},{"subj":"T851","pred":"source","obj":"craft-sa-dev"},{"subj":"T852","pred":"source","obj":"craft-sa-dev"},{"subj":"T853","pred":"source","obj":"craft-sa-dev"},{"subj":"T854","pred":"source","obj":"craft-sa-dev"},{"subj":"T855","pred":"source","obj":"craft-sa-dev"},{"subj":"T856","pred":"source","obj":"craft-sa-dev"},{"subj":"T857","pred":"source","obj":"craft-sa-dev"},{"subj":"T858","pred":"source","obj":"craft-sa-dev"},{"subj":"T859","pred":"source","obj":"craft-sa-dev"},{"subj":"T860","pred":"source","obj":"craft-sa-dev"},{"subj":"T861","pred":"source","obj":"craft-sa-dev"},{"subj":"T862","pred":"source","obj":"craft-sa-dev"},{"subj":"T863","pred":"source","obj":"craft-sa-dev"},{"subj":"T864","pred":"source","obj":"craft-sa-dev"},{"subj":"T865","pred":"source","obj":"craft-sa-dev"},{"subj":"T866","pred":"source","obj":"craft-sa-dev"},{"subj":"T867","pred":"source","obj":"craft-sa-dev"},{"subj":"T868","pred":"source","obj":"craft-sa-dev"},{"subj":"T869","pred":"source","obj":"craft-sa-dev"},{"subj":"T870","pred":"source","obj":"craft-sa-dev"},{"subj":"T871","pred":"source","obj":"craft-sa-dev"},{"subj":"T872","pred":"source","obj":"craft-sa-dev"},{"subj":"T873","pred":"source","obj":"craft-sa-dev"},{"subj":"T874","pred":"source","obj":"craft-sa-dev"},{"subj":"T875","pred":"source","obj":"craft-sa-dev"},{"subj":"T876","pred":"source","obj":"craft-sa-dev"},{"subj":"T877","pred":"source","obj":"craft-sa-dev"},{"subj":"T878","pred":"source","obj":"craft-sa-dev"},{"subj":"T879","pred":"source","obj":"craft-sa-dev"},{"subj":"T881","pred":"source","obj":"craft-sa-dev"},{"subj":"T882","pred":"source","obj":"craft-sa-dev"},{"subj":"T883","pred":"source","obj":"craft-sa-dev"},{"subj":"T884","pred":"source","obj":"craft-sa-dev"},{"subj":"T885","pred":"source","obj":"craft-sa-dev"},{"subj":"T886","pred":"source","obj":"craft-sa-dev"},{"subj":"T887","pred":"source","obj":"craft-sa-dev"},{"subj":"T888","pred":"source","obj":"craft-sa-dev"},{"subj":"T889","pred":"source","obj":"craft-sa-dev"},{"subj":"T890","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T557","span":{"begin":84,"end":89},"obj":"NCBITaxon:10088"},{"id":"T558","span":{"begin":90,"end":95},"obj":"PR:000005015"},{"id":"T559","span":{"begin":96,"end":100},"obj":"SO:0000704"},{"id":"T560","span":{"begin":104,"end":113},"obj":"UBERON:0000922"},{"id":"T561","span":{"begin":104,"end":125},"obj":"CL:0002322"},{"id":"T562","span":{"begin":154,"end":160},"obj":"SO:0001023"},{"id":"T563","span":{"begin":173,"end":178},"obj":"NCBITaxon:10088"},{"id":"T564","span":{"begin":214,"end":221},"obj":"NCBITaxon:33208"},{"id":"T565","span":{"begin":276,"end":285},"obj":"GO:0007566"},{"id":"T566","span":{"begin":286,"end":293},"obj":"UBERON:0000922"}],"attributes":[{"subj":"T557","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T558","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T559","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T560","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T561","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T562","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T563","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T564","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T565","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T566","pred":"source","obj":"craft-ca-core-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T629","span":{"begin":42,"end":49},"obj":"SO_EXT:sequence_deletion_process"},{"id":"T630","span":{"begin":63,"end":76},"obj":"SO_EXT:coding_sequence"},{"id":"T631","span":{"begin":84,"end":89},"obj":"NCBITaxon:10088"},{"id":"T632","span":{"begin":90,"end":95},"obj":"PR_EXT:000005015"},{"id":"T633","span":{"begin":96,"end":100},"obj":"SO_EXT:0000704"},{"id":"T634","span":{"begin":104,"end":113},"obj":"UBERON:0000922"},{"id":"T635","span":{"begin":104,"end":125},"obj":"CL:0002322"},{"id":"T636","span":{"begin":120,"end":125},"obj":"CL_GO_EXT:cell"},{"id":"T637","span":{"begin":147,"end":153},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T638","span":{"begin":154,"end":160},"obj":"SO_EXT:0001023"},{"id":"T639","span":{"begin":173,"end":178},"obj":"NCBITaxon:10088"},{"id":"T640","span":{"begin":189,"end":197},"obj":"GO_EXT:breeding"},{"id":"T641","span":{"begin":214,"end":221},"obj":"NCBITaxon:33208"},{"id":"T642","span":{"begin":251,"end":257},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T643","span":{"begin":276,"end":285},"obj":"GO:0007566"},{"id":"T644","span":{"begin":286,"end":293},"obj":"UBERON:0000922"}],"attributes":[{"subj":"T629","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T630","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T631","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T632","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T633","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T634","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T635","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T636","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T637","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T638","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T639","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T640","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T641","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T642","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T643","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T644","pred":"source","obj":"craft-ca-core-ex-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#93ecc1","default":true},{"id":"craft-ca-core-dev","color":"#eca793"},{"id":"craft-ca-core-ex-dev","color":"#9398ec"}]}]}}