PMC:1382200 / 881-1254 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T833","span":{"begin":42,"end":49},"obj":"VBD"},{"id":"T835","span":{"begin":5,"end":8},"obj":"DT"},{"id":"T836","span":{"begin":9,"end":21},"obj":"NNS"},{"id":"T837","span":{"begin":22,"end":31},"obj":"VBN"},{"id":"T838","span":{"begin":32,"end":37},"obj":"RB"},{"id":"T839","span":{"begin":37,"end":39},"obj":","},{"id":"T840","span":{"begin":39,"end":41},"obj":"PRP"},{"id":"T841","span":{"begin":50,"end":52},"obj":"DT"},{"id":"T842","span":{"begin":70,"end":76},"obj":"NN"},{"id":"T843","span":{"begin":53,"end":62},"obj":"JJ"},{"id":"T844","span":{"begin":63,"end":69},"obj":"NN"},{"id":"T845","span":{"begin":77,"end":79},"obj":"IN"},{"id":"T846","span":{"begin":80,"end":83},"obj":"DT"},{"id":"T847","span":{"begin":96,"end":100},"obj":"NN"},{"id":"T848","span":{"begin":84,"end":89},"obj":"NN"},{"id":"T849","span":{"begin":90,"end":95},"obj":"NN"},{"id":"T850","span":{"begin":101,"end":103},"obj":"IN"},{"id":"T851","span":{"begin":104,"end":113},"obj":"JJ"},{"id":"T852","span":{"begin":120,"end":125},"obj":"NNS"},{"id":"T853","span":{"begin":114,"end":119},"obj":"VBZ"},{"id":"T854","span":{"begin":126,"end":129},"obj":"CC"},{"id":"T855","span":{"begin":130,"end":141},"obj":"VBN"},{"id":"T856","span":{"begin":142,"end":146},"obj":"DT"},{"id":"T857","span":{"begin":154,"end":160},"obj":"NN"},{"id":"T858","span":{"begin":147,"end":153},"obj":"NN"},{"id":"T859","span":{"begin":161,"end":168},"obj":"IN"},{"id":"T860","span":{"begin":169,"end":172},"obj":"DT"},{"id":"T861","span":{"begin":179,"end":187},"obj":"NN"},{"id":"T862","span":{"begin":173,"end":178},"obj":"NN"},{"id":"T863","span":{"begin":187,"end":188},"obj":"."},{"id":"T864","span":{"begin":188,"end":294},"obj":"sentence"},{"id":"T865","span":{"begin":189,"end":197},"obj":"NN"},{"id":"T866","span":{"begin":222,"end":228},"obj":"VBD"},{"id":"T867","span":{"begin":198,"end":200},"obj":"IN"},{"id":"T868","span":{"begin":201,"end":213},"obj":"JJ"},{"id":"T869","span":{"begin":214,"end":221},"obj":"NNS"},{"id":"T870","span":{"begin":229,"end":231},"obj":"TO"},{"id":"T871","span":{"begin":232,"end":239},"obj":"VB"},{"id":"T872","span":{"begin":240,"end":250},"obj":"JJ"},{"id":"T873","span":{"begin":263,"end":272},"obj":"NN"},{"id":"T874","span":{"begin":251,"end":257},"obj":"NN"},{"id":"T875","span":{"begin":258,"end":262},"obj":"JJ"},{"id":"T876","span":{"begin":273,"end":275},"obj":"CC"},{"id":"T877","span":{"begin":276,"end":285},"obj":"VBN"},{"id":"T878","span":{"begin":286,"end":293},"obj":"NNS"},{"id":"T879","span":{"begin":293,"end":294},"obj":"."},{"id":"T881","span":{"begin":295,"end":296},"obj":"DT"},{"id":"T882","span":{"begin":319,"end":327},"obj":"NN"},{"id":"T883","span":{"begin":297,"end":303},"obj":"VBN"},{"id":"T884","span":{"begin":304,"end":307},"obj":"NN"},{"id":"T885","span":{"begin":308,"end":318},"obj":"NN"},{"id":"T886","span":{"begin":332,"end":343},"obj":"VBN"},{"id":"T887","span":{"begin":328,"end":331},"obj":"VBD"},{"id":"T888","span":{"begin":343,"end":345},"obj":","},{"id":"T889","span":{"begin":345,"end":348},"obj":"CC"},{"id":"T890","span":{"begin":349,"end":359},"obj":"JJ"}],"relations":[{"id":"R155","pred":"det","subj":"T835","obj":"T836"},{"id":"R157","pred":"acl","subj":"T837","obj":"T836"},{"id":"R158","pred":"advmod","subj":"T838","obj":"T837"},{"id":"R159","pred":"punct","subj":"T839","obj":"T833"},{"id":"R160","pred":"nsubj","subj":"T840","obj":"T833"},{"id":"R161","pred":"det","subj":"T841","obj":"T842"},{"id":"R162","pred":"dobj","subj":"T842","obj":"T833"},{"id":"R163","pred":"amod","subj":"T843","obj":"T842"},{"id":"R164","pred":"compound","subj":"T844","obj":"T842"},{"id":"R165","pred":"prep","subj":"T845","obj":"T842"},{"id":"R166","pred":"det","subj":"T846","obj":"T847"},{"id":"R167","pred":"pobj","subj":"T847","obj":"T845"},{"id":"R168","pred":"compound","subj":"T848","obj":"T847"},{"id":"R169","pred":"compound","subj":"T849","obj":"T847"},{"id":"R170","pred":"prep","subj":"T850","obj":"T833"},{"id":"R171","pred":"amod","subj":"T851","obj":"T852"},{"id":"R173","pred":"nmod","subj":"T853","obj":"T852"},{"id":"R174","pred":"cc","subj":"T854","obj":"T833"},{"id":"R175","pred":"conj","subj":"T855","obj":"T833"},{"id":"R176","pred":"det","subj":"T856","obj":"T857"},{"id":"R178","pred":"compound","subj":"T858","obj":"T857"},{"id":"R179","pred":"prep","subj":"T859","obj":"T855"},{"id":"R180","pred":"det","subj":"T860","obj":"T861"},{"id":"R182","pred":"compound","subj":"T862","obj":"T861"},{"id":"R183","pred":"punct","subj":"T863","obj":"T833"},{"id":"R184","pred":"nsubj","subj":"T865","obj":"T866"},{"id":"R185","pred":"prep","subj":"T867","obj":"T865"},{"id":"R186","pred":"amod","subj":"T868","obj":"T869"},{"id":"R187","pred":"pobj","subj":"T869","obj":"T867"},{"id":"R188","pred":"aux","subj":"T870","obj":"T871"},{"id":"R189","pred":"xcomp","subj":"T871","obj":"T866"},{"id":"R190","pred":"amod","subj":"T872","obj":"T873"},{"id":"R191","pred":"dobj","subj":"T873","obj":"T871"},{"id":"R192","pred":"nmod","subj":"T874","obj":"T873"},{"id":"R193","pred":"amod","subj":"T875","obj":"T873"},{"id":"R194","pred":"cc","subj":"T876","obj":"T873"},{"id":"R195","pred":"amod","subj":"T877","obj":"T878"},{"id":"R196","pred":"conj","subj":"T878","obj":"T873"},{"id":"R197","pred":"punct","subj":"T879","obj":"T866"},{"id":"R198","pred":"det","subj":"T881","obj":"T882"},{"id":"R199","pred":"nsubjpass","subj":"T882","obj":"T886"},{"id":"R200","pred":"amod","subj":"T883","obj":"T882"},{"id":"R201","pred":"compound","subj":"T884","obj":"T882"},{"id":"R202","pred":"compound","subj":"T885","obj":"T882"},{"id":"R203","pred":"auxpass","subj":"T887","obj":"T886"},{"id":"R204","pred":"punct","subj":"T888","obj":"T886"},{"id":"R205","pred":"cc","subj":"T889","obj":"T886"},{"id":"R172","pred":"pobj","subj":"T852","obj":"T850"},{"id":"R177","pred":"dobj","subj":"T857","obj":"T855"},{"id":"R181","pred":"pobj","subj":"T861","obj":"T859"}],"text":"ween the alternatives described above, we deleted an essential coding region in the mouse Capn2 gene in embryonic stems cells and transmitted this mutant allele through the mouse germline. Breeding of heterozygous animals failed to produce homozygous mutant live offspring or implanted embryos. A nested PCR genotyping protocol was established, and homozygous preimplantati"}

{"project":"craft-ca-core-dev","denotations":[{"id":"T557","span":{"begin":84,"end":89},"obj":"NCBITaxon:10088"},{"id":"T558","span":{"begin":90,"end":95},"obj":"PR:000005015"},{"id":"T559","span":{"begin":96,"end":100},"obj":"SO:0000704"},{"id":"T560","span":{"begin":104,"end":113},"obj":"UBERON:0000922"},{"id":"T561","span":{"begin":104,"end":125},"obj":"CL:0002322"},{"id":"T562","span":{"begin":154,"end":160},"obj":"SO:0001023"},{"id":"T563","span":{"begin":173,"end":178},"obj":"NCBITaxon:10088"},{"id":"T564","span":{"begin":214,"end":221},"obj":"NCBITaxon:33208"},{"id":"T565","span":{"begin":276,"end":285},"obj":"GO:0007566"},{"id":"T566","span":{"begin":286,"end":293},"obj":"UBERON:0000922"}],"text":"ween the alternatives described above, we deleted an essential coding region in the mouse Capn2 gene in embryonic stems cells and transmitted this mutant allele through the mouse germline. Breeding of heterozygous animals failed to produce homozygous mutant live offspring or implanted embryos. A nested PCR genotyping protocol was established, and homozygous preimplantati"}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T629","span":{"begin":42,"end":49},"obj":"SO_EXT:sequence_deletion_process"},{"id":"T630","span":{"begin":63,"end":76},"obj":"SO_EXT:coding_sequence"},{"id":"T631","span":{"begin":84,"end":89},"obj":"NCBITaxon:10088"},{"id":"T632","span":{"begin":90,"end":95},"obj":"PR_EXT:000005015"},{"id":"T633","span":{"begin":96,"end":100},"obj":"SO_EXT:0000704"},{"id":"T634","span":{"begin":104,"end":113},"obj":"UBERON:0000922"},{"id":"T635","span":{"begin":104,"end":125},"obj":"CL:0002322"},{"id":"T636","span":{"begin":120,"end":125},"obj":"CL_GO_EXT:cell"},{"id":"T637","span":{"begin":147,"end":153},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T638","span":{"begin":154,"end":160},"obj":"SO_EXT:0001023"},{"id":"T639","span":{"begin":173,"end":178},"obj":"NCBITaxon:10088"},{"id":"T640","span":{"begin":189,"end":197},"obj":"GO_EXT:breeding"},{"id":"T641","span":{"begin":214,"end":221},"obj":"NCBITaxon:33208"},{"id":"T642","span":{"begin":251,"end":257},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T643","span":{"begin":276,"end":285},"obj":"GO:0007566"},{"id":"T644","span":{"begin":286,"end":293},"obj":"UBERON:0000922"}],"text":"ween the alternatives described above, we deleted an essential coding region in the mouse Capn2 gene in embryonic stems cells and transmitted this mutant allele through the mouse germline. Breeding of heterozygous animals failed to produce homozygous mutant live offspring or implanted embryos. A nested PCR genotyping protocol was established, and homozygous preimplantati"}