PMC:1253828 / 20121-21564 JSONTXT

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The basal level was reduced in livers from mock- and cadmium-treated Mtf1Mx-cre mice, indicating that MTF-1 is important for the basal expression of Sepw1.\nThree MRE core consensus sequences were found in the region upstream of the mouse Sepw1 transcription start (Figure 2c). Two of them in opposite orientation overlap almost completely proximal to the transcription start (MRE1, −40 bp), the third one is located further upstream (MRE2, −527 bp). Specific binding of MTF-1 to Sepw1 MRE1 but not MRE2 oligonucleotide was observed in EMSA with liver protein extract from an Mtf1loxP control mouse (Figure 2d). As a control, no binding to MRE1 was detected with extract from a pI–pC-induced Mtf1Mx-cre mouse, confirming that the bandshift was indeed dependent on the presence of 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