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PMC:1208879 / 25700-27583 JSONTXT

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Id Subject Object Predicate Lexical cue
T7133 1403-1409 NN denotes mutant
T7132 1421-1433 NN denotes substitution
T7131 1399-1402 DT denotes the
T7130 1390-1398 VBZ denotes contains
T7129 1384-1389 WDT denotes which
T7128 1382-1384 , denotes ,
T7127 1381-1382 SYM denotes '
T7126 1380-1381 CD denotes 3
T7125 1379-1380 HYPH denotes -
T7124 1373-1376 NN denotes TTG
T7123 1369-1372 NN denotes GTA
T7122 1365-1368 NN denotes CCA
T7121 1361-1364 NN denotes TGG
T7120 1357-1360 NN denotes TCT
T7119 1353-1356 NN denotes TAA
T7118 1349-1352 NN denotes CCG
T7117 1345-1348 NN denotes TTA
T7116 1341-1344 NN denotes TTT
T7115 1337-1340 NN denotes CGC
T7114 1333-1336 NN denotes GGC
T7113 1329-1332 NN denotes TGC
T7112 1325-1328 NN denotes TTT
T7111 1324-1325 HYPH denotes -
T7110 1323-1324 SYM denotes '
T7109 1322-1323 CD denotes 5
T7108 1377-1379 NN denotes AG
T7107 1316-1321 NN denotes ST006
T7106 1305-1308 NN denotes PCR
T7105 1297-1304 JJ denotes reverse
T7104 1309-1315 NN denotes primer
T7103 1293-1296 DT denotes the
T7102 1287-1292 VBG denotes using
T7101 1283-1286 CC denotes but
T7100 1281-1283 , denotes ,
T7099 1264-1271 NN denotes PAX6PST
T7098 1272-1281 NN denotes construct
T7097 1260-1263 DT denotes the
T7096 1257-1259 IN denotes as
T7095 1248-1252 JJ denotes same
T7094 1253-1256 NN denotes way
T7093 1244-1247 DT denotes the
T7092 1241-1243 IN denotes in
T7091 1227-1230 VBD denotes was
T7090 1225-1226 -RRB- denotes )
T7089 1220-1225 NN denotes Q422R
T7088 1219-1220 -LRB- denotes (
T7087 1217-1218 NN denotes G
T7086 1216-1217 SYM denotes >
T7085 1211-1216 NN denotes 1627A
T7084 1202-1210 NN denotes mutation
T7083 1198-1201 DT denotes the
T7082 1187-1197 VBG denotes containing
T7081 1231-1240 VBN denotes generated
T7080 1166-1169 NN denotes PST
T7079 1170-1176 NN denotes domain
T7078 1161-1165 NN denotes PAX6
T7077 1177-1186 NN denotes construct
T7076 1159-1160 DT denotes A
T7075 1158-1447 sentence denotes A PAX6 PST domain construct containing the mutation 1627A>G (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).
T7074 1157-1158 . denotes .
T7073 1142-1146 VBN denotes been
T7072 1138-1141 VBD denotes had
T7071 1131-1137 NNS denotes errors
T7070 1128-1130 DT denotes no
T7069 1147-1157 VBN denotes introduced
T7068 1123-1127 IN denotes that
T7067 1117-1122 VB denotes check
T7066 1114-1116 TO denotes to
T7065 1099-1103 VBD denotes were
T7064 1091-1098 NNS denotes linkers
T7063 1086-1090 IN denotes with
T7062 1083-1085 CC denotes or
T7061 1079-1082 NN denotes PCR
T7060 1076-1078 IN denotes by
T7059 1066-1075 VBN denotes generated
T7058 1104-1113 VBN denotes sequenced
T7057 1056-1065 NNS denotes fragments
T7056 1052-1055 DT denotes All
T7055 1051-1158 sentence denotes All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.
T7054 1050-1051 . denotes .
T7053 1033-1044 NN denotes restriction
T7052 1029-1032 CD denotes two
T7051 1045-1050 NNS denotes sites
T7050 1025-1028 DT denotes the
T7049 1017-1024 IN denotes between
T7048 1000-1009 JJ denotes synthetic
T7047 1010-1016 NN denotes linker
T7046 998-999 DT denotes a
T7045 988-997 VBG denotes inserting
T7044 984-987 CC denotes and
T7043 982-984 , denotes ,
T7042 965-966 HYPH denotes -
T7041 966-974 JJ denotes terminal
T7040 964-965 NN denotes C
T7039 975-982 NN denotes peptide
T7038 960-963 DT denotes the
T7037 956-959 RP denotes out
T7036 951-955 VB denotes drop
T7035 948-950 TO denotes to
T7034 943-947 NN denotes NotI
T7033 939-942 CC denotes and
T7032 934-938 NN denotes NdeI
T7031 929-933 IN denotes with
T7030 921-928 NN denotes PAX6PST
T7029 913-920 VBG denotes cutting
T7028 910-912 IN denotes by
T7027 901-904 VBD denotes was
T7026 905-909 VBN denotes made
T7025 896-900 DT denotes This
T7024 895-1051 sentence denotes This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites.
T7023 894-895 . denotes .
T7022 893-894 -RRB- denotes )
T7021 890-893 CD denotes 390
T7020 889-890 SYM denotes –
T7019 886-889 CD denotes 278
T7018 885-886 -LRB- denotes (
T7017 867-868 HYPH denotes -
T7016 868-876 JJ denotes terminal
T7015 866-867 NN denotes C
T7014 877-884 NN denotes peptide
T7013 862-865 DT denotes the
T7012 856-861 CC denotes minus
T7011 845-848 NN denotes PST
T7010 849-855 NN denotes domain
T7009 841-844 DT denotes the
T7008 832-840 VBZ denotes contains
T7007 828-829 HYPH denotes -
T7006 829-831 NN denotes CT
T7005 821-828 NN denotes PAX6PST
T7004 820-895 sentence denotes PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390).
T7003 819-820 . denotes .
T7002 818-819 -RRB- denotes )
T7001 817-818 NN denotes R
T7000 816-817 -LRB- denotes (
T6999 810-815 NN denotes ST005
T6998 806-809 CC denotes and
T6997 804-805 SYM denotes '
T6996 803-804 CD denotes 3
T6995 802-803 HYPH denotes -
T6994 796-799 NN denotes TCA
T6993 792-795 NN denotes GAC
T6992 788-791 NN denotes CAG
T6991 784-787 NN denotes CAA
T6990 780-783 NN denotes CTT
T6989 776-779 NN denotes CCA
T6988 772-775 NN denotes CTA
T6987 768-771 NN denotes CGA
T6986 764-767 NN denotes AGT
T6985 760-763 NN denotes AAA
T6984 759-760 HYPH denotes -
T6983 758-759 SYM denotes '
T6982 757-758 CD denotes 5
T6981 755-756 -RRB- denotes )
T6980 754-755 NN denotes F
T6979 753-754 -LRB- denotes (
T6978 800-802 NN denotes TT
T6977 747-752 NN denotes ST004
T6976 742-746 VBD denotes were
T6975 734-741 NNS denotes Primers
T6974 733-820 sentence denotes Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R).
T6973 732-733 . denotes .
T6972 731-732 -RRB- denotes )
T6971 728-731 CD denotes 422
T6970 727-728 SYM denotes –
T6969 724-727 CD denotes 391
T6968 723-724 -LRB- denotes (
T6967 717-722 JJ denotes alone
T6966 699-700 HYPH denotes -
T6965 700-708 JJ denotes terminal
T6964 698-699 NN denotes C
T6963 690-697 VBN denotes defined
T6962 684-689 RB denotes newly
T6961 709-716 NN denotes peptide
T6960 680-683 DT denotes the
T6959 671-679 VBZ denotes contains
T6958 663-670 NN denotes PAX6CTP
T6957 662-733 sentence denotes PAX6CTP contains the newly defined C-terminal peptide alone (391–422).
T6956 661-662 . denotes .
T6955 660-661 SYM denotes '
T6954 659-660 CD denotes 3
T6953 658-659 HYPH denotes -
T6952 651-654 NN denotes GTA
T6951 647-650 NN denotes CCA
T6950 643-646 NN denotes TGG
T6949 639-642 NN denotes TCT
T6948 635-638 NN denotes TAA
T6947 631-634 NN denotes CTG
T6946 627-630 NN denotes TTA
T6945 623-626 NN denotes TTT
T6944 619-622 NN denotes GGC
T6943 615-618 NN denotes TGC
T6942 611-614 NN denotes TTT
T6941 610-611 HYPH denotes -
T6940 609-610 SYM denotes '
T6939 608-609 CD denotes 5
T6938 606-607 -RRB- denotes )
T6937 605-606 NN denotes R
T6936 604-605 -LRB- denotes (
T6935 655-658 NN denotes TTG
T6934 598-603 NN denotes ST005
T6933 594-597 CC denotes and
T6932 592-593 SYM denotes '
T6931 591-592 CD denotes 3
T6930 590-591 HYPH denotes -
T6928 581-584 NN denotes CCT
T6927 577-580 NN denotes ACA
T6926 573-576 NN denotes AAC
T6925 569-572 NN denotes AGC
T6924 565-568 NN denotes GCC
T6923 561-564 NN denotes ACT
T6922 557-560 NN denotes TCG
T6921 553-556 NN denotes AGT
T6920 549-552 NN denotes AAA
T6919 548-549 HYPH denotes -
T6918 547-548 SYM denotes '
T6909 516-662 sentence denotes Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'.
T6908 515-516 . denotes .
T6907 514-515 -RRB- denotes )
T6906 502-506 NN denotes PAX6
T6905 494-495 HYPH denotes -
T6904 495-501 NN denotes length
T6903 490-494 JJ denotes full
T6902 507-514 NN denotes protein
T6901 486-489 DT denotes the
T6900 483-485 IN denotes of
T6899 479-482 CD denotes 422
T6898 478-479 SYM denotes –
T6897 469-474 NNS denotes acids
T6896 463-468 NN denotes amino
T6895 475-478 CD denotes 278
T6894 462-463 -LRB- denotes (
T6893 451-454 NN denotes PST
T6892 445-450 JJ denotes whole
T6891 455-461 NN denotes domain
T6890 441-444 DT denotes the
T6889 432-440 VBZ denotes contains
T6888 424-431 NN denotes PAX6PST
T6887 423-516 sentence denotes PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein).
T6886 422-423 . denotes .
T6885 408-415 NN denotes binding
T6884 404-407 NN denotes DNA
T6883 399-403 NN denotes GAL4
T6882 393-398 NN denotes yeast
T6881 416-422 NN denotes domain
T6880 389-392 DT denotes the
T6879 386-388 IN denotes to
T6878 380-385 VBN denotes fused
T6877 372-379 NN denotes protein
T6876 370-371 DT denotes a
T6875 360-369 VBZ denotes generates
T6874 354-359 WDT denotes which
T6873 352-354 , denotes ,
T6872 351-352 -RRB- denotes )
T6871 341-351 NNP denotes Invitrogen
T6870 339-341 , denotes ,
T6869 325-326 HYPH denotes -
T6868 326-332 NN denotes Hybrid
T6867 322-325 CD denotes Two
T6866 313-321 NNP denotes ProQuest
T6865 333-339 NN denotes System
T6864 312-313 -LRB- denotes (
T6863 294-304 NN denotes expression
T6862 287-293 NN denotes pDBLeu
T6861 305-311 NN denotes vector
T6860 283-286 DT denotes the
T6859 280-282 IN denotes in
T6858 264-268 NN denotes cDNA
T6857 259-263 NN denotes PAX6
T6856 269-279 NNS denotes constructs
T6855 253-258 CD denotes three
T6854 248-252 VB denotes make
T6853 245-247 TO denotes to
T6852 235-239 VBD denotes were
T6851 240-244 VBN denotes used
T6850 213-223 NN denotes subcloning
T6849 209-212 CC denotes and
T6848 205-208 NN denotes PCR
T6847 224-234 NNS denotes techniques
T6846 196-204 JJ denotes Standard
T6845 195-423 sentence denotes Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain.
T6844 194-195 . denotes .
T6843 193-194 -RRB- denotes ]
T6842 191-193 CD denotes 27
T6841 190-191 -LRB- denotes [
T6840 181-184 NN denotes web
T6839 164-171 NN denotes Variant
T6838 156-163 JJ denotes Allelic
T6837 172-180 NN denotes Database
T6836 151-155 NN denotes PAX6
T6835 145-150 JJ denotes Human
T6834 185-189 NN denotes site
T6833 141-144 DT denotes the
T6832 136-140 IN denotes from
T6831 126-135 JJ denotes available
T6830 106-115 NN denotes reference
T6829 98-105 NN denotes protein
T6828 94-97 CC denotes and
T6827 84-88 NN denotes PAX6
T6826 89-93 NN denotes cDNA
T6825 78-83 JJ denotes human
T6824 116-125 NNS denotes sequences
T6823 74-77 DT denotes the
T6822 71-73 IN denotes on
T6821 65-70 VBN denotes based
T6820 62-64 VBZ denotes is
T6819 47-51 NN denotes acid
T6818 41-46 NN denotes amino
T6817 37-40 CC denotes and
T6816 32-36 NN denotes cDNA
T6815 52-61 NN denotes numbering
T6814 28-31 DT denotes All
T6813 27-195 sentence denotes All cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27].
T6812 9-10 HYPH denotes -
T6811 10-16 NN denotes hybrid
T6810 6-9 CD denotes two
T6809 17-27 NNS denotes constructs
T6808 0-5 NN denotes Yeast
T7154 1522-1523 -RRB- denotes )
T7247 1882-1883 . denotes .
T7246 1870-1873 VBD denotes was
T7245 1874-1882 VBN denotes inserted
T7244 1854-1860 NN denotes mutant
T7243 1861-1869 NN denotes sequence
T7242 1850-1853 DT denotes the
T7241 1846-1849 CC denotes and
T7240 1844-1846 , denotes ,
T7239 1827-1828 HYPH denotes -
T7238 1828-1836 JJ denotes terminal
T7237 1826-1827 NN denotes C
T7236 1819-1825 JJ denotes normal
T7235 1837-1844 NN denotes peptide
T7234 1815-1818 DT denotes the
T7233 1807-1814 VB denotes release
T7232 1804-1806 TO denotes to
T7231 1799-1803 NN denotes NotI
T7230 1795-1798 CC denotes and
T7229 1790-1794 NN denotes NdeI
T7228 1785-1789 IN denotes with
T7227 1777-1780 VBD denotes was
T7226 1781-1784 VBN denotes cut
T7225 1759-1766 NN denotes PAX6PST
T7224 1767-1776 NN denotes construct
T7223 1755-1758 DT denotes The
T7222 1754-1883 sentence denotes The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted.
T7221 1753-1754 . denotes .
T7220 1752-1753 SYM denotes '
T7219 1751-1752 CD denotes 3
T7218 1750-1751 HYPH denotes -
T7217 1744-1747 NN denotes TGT
T7216 1740-1743 NN denotes CAT
T7215 1736-1739 NN denotes CTA
T7214 1732-1735 NN denotes AGT
T7213 1728-1731 NN denotes TCC
T7212 1724-1727 NN denotes CCA
T7211 1720-1723 NN denotes CAT
T7210 1716-1719 NN denotes CCG
T7209 1712-1715 NN denotes CGG
T7208 1708-1711 NN denotes TTG
T7207 1707-1708 HYPH denotes -
T7206 1706-1707 SYM denotes '
T7205 1705-1706 CD denotes 5
T7204 1703-1704 -RRB- denotes )
T7203 1702-1703 NN denotes R
T7202 1701-1702 -LRB- denotes (
T7201 1748-1750 NN denotes TC
T7200 1695-1700 NN denotes ST031
T7199 1691-1694 CC denotes and
T7198 1689-1690 SYM denotes '
T7197 1688-1689 CD denotes 3
T7196 1687-1688 HYPH denotes -
T7195 1682-1685 NN denotes ACA
T7194 1678-1681 NN denotes GAC
T7193 1674-1677 NN denotes GCA
T7192 1670-1673 NN denotes TAT
T7191 1666-1669 NN denotes ACA
T7190 1662-1665 NN denotes CCC
T7189 1661-1662 HYPH denotes -
T7188 1660-1661 SYM denotes '
T7187 1659-1660 CD denotes 5
T7186 1657-1658 -RRB- denotes )
T7185 1656-1657 NN denotes F
T7184 1655-1656 -LRB- denotes (
T7183 1686-1687 NN denotes C
T7182 1649-1654 NN denotes ST015
T7181 1644-1648 VBD denotes were
T7180 1636-1643 NNS denotes Primers
T7179 1635-1754 sentence denotes Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'.
T7178 1634-1635 . denotes .
T7177 1633-1634 -RRB- denotes )
T7176 1620-1623 NNP denotes van
T7175 1618-1619 NNP denotes V
T7174 1624-1633 NNP denotes Heyningen
T7173 1613-1617 NNP denotes Prof
T7172 1609-1612 CC denotes and
T7171 1596-1597 NNP denotes K
T7170 1598-1608 NNP denotes Williamson
T7169 1593-1595 NNP denotes Dr
T7168 1588-1592 IN denotes from
T7167 1581-1582 DT denotes a
T7166 1583-1587 NN denotes gift
T7165 1580-1581 -LRB- denotes (
T7164 1576-1579 NN denotes RNA
T7163 1564-1575 VBN denotes transcribed
T7162 1556-1563 JJ denotes reverse
T7161 1551-1555 IN denotes from
T7160 1547-1550 NN denotes PCR
T7159 1544-1546 IN denotes by
T7158 1529-1533 VBD denotes were
T7157 1527-1528 -RRB- denotes ]
T7156 1525-1527 CD denotes 12
T7155 1524-1525 -LRB- denotes [
T7153 1517-1522 NN denotes X423L
T7152 1516-1517 -LRB- denotes (
T7151 1507-1515 NN denotes 1629insT
T7150 1503-1506 CC denotes and
T7149 1501-1502 -RRB- denotes ]
T7148 1499-1501 CD denotes 28
T7147 1498-1499 -LRB- denotes [
T7146 1488-1497 NN denotes 1615del10
T7145 1478-1487 NNS denotes mutations
T7144 1474-1477 DT denotes the
T7143 1463-1473 VBG denotes containing
T7142 1534-1543 VBN denotes generated
T7141 1453-1462 NNS denotes sequences
T7140 1448-1452 NN denotes cDNA
T7139 1447-1635 sentence denotes cDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen).
T7138 1446-1447 . denotes .
T7137 1445-1446 -RRB- denotes )
T7136 1435-1445 JJ denotes underlined
T7135 1434-1435 -LRB- denotes (
T7134 1410-1420 NN denotes nucleotide
T6917 546-547 CD denotes 5
T6916 544-545 -RRB- denotes )
T6915 537-544 JJ denotes forward
T6914 536-537 -LRB- denotes (
T6913 589-590 NN denotes C
T6912 530-535 NN denotes ST001
T6911 525-529 VBD denotes were
T6910 517-524 NNS denotes Primers
T6929 585-588 NN denotes AGT
R4202 T6808 T6809 nmod Yeast,constructs
R4203 T6810 T6811 nummod two,hybrid
R4204 T6811 T6809 compound hybrid,constructs
R4205 T6812 T6811 punct -,hybrid
R4206 T6814 T6815 det All,numbering
R4207 T6815 T6820 nsubj numbering,is
R4208 T6816 T6815 nmod cDNA,numbering
R4209 T6817 T6816 cc and,cDNA
R4210 T6818 T6819 compound amino,acid
R4211 T6819 T6816 conj acid,cDNA
R4212 T6821 T6820 prep based,is
R4213 T6822 T6821 prep on,based
R4214 T6823 T6824 det the,sequences
R4215 T6824 T6822 pobj sequences,on
R4216 T6825 T6826 amod human,cDNA
R4217 T6826 T6824 nmod cDNA,sequences
R4218 T6827 T6826 nmod PAX6,cDNA
R4219 T6828 T6826 cc and,cDNA
R4220 T6829 T6830 compound protein,reference
R4221 T6830 T6826 conj reference,cDNA
R4222 T6831 T6824 amod available,sequences
R4223 T6832 T6831 prep from,available
R4224 T6833 T6834 det the,site
R4225 T6834 T6832 pobj site,from
R4226 T6835 T6836 amod Human,PAX6
R4227 T6836 T6837 nmod PAX6,Database
R4228 T6837 T6834 compound Database,site
R4229 T6838 T6839 amod Allelic,Variant
R4230 T6839 T6837 compound Variant,Database
R4231 T6840 T6834 compound web,site
R4232 T6841 T6842 punct [,27
R4233 T6842 T6821 parataxis 27,based
R4234 T6843 T6842 punct ],27
R4235 T6844 T6820 punct .,is
R4236 T6846 T6847 amod Standard,techniques
R4237 T6847 T6851 nsubjpass techniques,used
R4238 T6848 T6847 nmod PCR,techniques
R4239 T6849 T6848 cc and,PCR
R4240 T6850 T6848 conj subcloning,PCR
R4241 T6852 T6851 auxpass were,used
R4242 T6853 T6854 aux to,make
R4243 T6854 T6851 advcl make,used
R4244 T6855 T6856 nummod three,constructs
R4245 T6856 T6854 dobj constructs,make
R4246 T6857 T6858 compound PAX6,cDNA
R4247 T6858 T6856 compound cDNA,constructs
R4248 T6859 T6854 prep in,make
R4249 T6860 T6861 det the,vector
R4250 T6861 T6859 pobj vector,in
R4251 T6862 T6861 compound pDBLeu,vector
R4252 T6863 T6861 compound expression,vector
R4253 T6864 T6865 punct (,System
R4254 T6865 T6861 parataxis System,vector
R4255 T6866 T6865 nmod ProQuest,System
R4256 T6867 T6868 nummod Two,Hybrid
R4257 T6868 T6865 compound Hybrid,System
R4258 T6869 T6868 punct -,Hybrid
R4259 T6870 T6865 punct ", ",System
R4260 T6871 T6865 npadvmod Invitrogen,System
R4261 T6872 T6865 punct ),System
R4262 T6873 T6861 punct ", ",vector
R4263 T6874 T6875 dep which,generates
R4264 T6875 T6861 relcl generates,vector
R4265 T6876 T6877 det a,protein
R4266 T6877 T6875 dobj protein,generates
R4267 T6878 T6877 acl fused,protein
R4268 T6879 T6878 prep to,fused
R4269 T6880 T6881 det the,domain
R4270 T6881 T6879 pobj domain,to
R4271 T6882 T6881 compound yeast,domain
R4272 T6883 T6881 compound GAL4,domain
R4273 T6884 T6885 compound DNA,binding
R4274 T6885 T6881 compound binding,domain
R4275 T6886 T6851 punct .,used
R4276 T6888 T6889 nsubj PAX6PST,contains
R4277 T6890 T6891 det the,domain
R4278 T6891 T6889 dobj domain,contains
R4279 T6892 T6891 amod whole,domain
R4280 T6893 T6891 compound PST,domain
R4281 T6894 T6895 punct (,278
R4282 T6895 T6889 parataxis 278,contains
R4283 T6896 T6895 nmod amino,278
R4284 T6897 T6895 nmod acids,278
R4285 T6898 T6899 punct –,422
R4286 T6899 T6895 prep 422,278
R4287 T6900 T6895 prep of,278
R4288 T6901 T6902 det the,protein
R4289 T6902 T6900 pobj protein,of
R4290 T6903 T6904 amod full,length
R4291 T6904 T6902 compound length,protein
R4292 T6905 T6904 punct -,length
R4293 T6906 T6902 compound PAX6,protein
R4294 T6907 T6895 punct ),278
R4295 T6908 T6889 punct .,contains
R4296 T6910 T6911 nsubj Primers,were
R4297 T6912 T6913 nmod ST001,C
R4298 T6913 T6911 attr C,were
R4299 T6914 T6915 punct (,forward
R4300 T6915 T6913 parataxis forward,C
R4301 T6916 T6915 punct ),forward
R4302 T6917 T6913 nummod 5,C
R4303 T6918 T6917 punct ',5
R4304 T6919 T6913 punct -,C
R4305 T6920 T6913 compound AAA,C
R4306 T6921 T6913 compound AGT,C
R4307 T6922 T6913 compound TCG,C
R4308 T6923 T6913 compound ACT,C
R4309 T6924 T6913 compound GCC,C
R4310 T6925 T6913 compound AGC,C
R4311 T6926 T6913 compound AAC,C
R4312 T6927 T6913 compound ACA,C
R4313 T6928 T6913 compound CCT,C
R4314 T6929 T6913 compound AGT,C
R4315 T6930 T6913 punct -,C
R4316 T6931 T6913 nummod 3,C
R4317 T6932 T6913 punct ',C
R4318 T6933 T6913 cc and,C
R4319 T6934 T6935 nmod ST005,TTG
R4320 T6935 T6913 conj TTG,C
R4321 T6936 T6937 punct (,R
R4322 T6937 T6935 parataxis R,TTG
R4323 T6938 T6937 punct ),R
R4324 T6939 T6935 nummod 5,TTG
R4325 T6940 T6939 punct ',5
R4326 T6941 T6935 punct -,TTG
R4327 T6942 T6935 compound TTT,TTG
R4328 T6943 T6935 compound TGC,TTG
R4329 T6944 T6935 compound GGC,TTG
R4330 T6945 T6935 compound TTT,TTG
R4331 T6946 T6935 compound TTA,TTG
R4332 T6947 T6935 compound CTG,TTG
R4333 T6948 T6935 compound TAA,TTG
R4334 T6949 T6935 compound TCT,TTG
R4335 T6950 T6935 compound TGG,TTG
R4336 T6951 T6935 compound CCA,TTG
R4337 T6952 T6935 compound GTA,TTG
R4338 T6953 T6935 punct -,TTG
R4339 T6954 T6935 nummod 3,TTG
R4340 T6955 T6935 punct ',TTG
R4341 T6956 T6911 punct .,were
R4342 T6958 T6959 nsubj PAX6CTP,contains
R4343 T6960 T6961 det the,peptide
R4344 T6961 T6959 dobj peptide,contains
R4345 T6962 T6963 advmod newly,defined
R4346 T6963 T6961 amod defined,peptide
R4347 T6964 T6965 npadvmod C,terminal
R4348 T6965 T6961 amod terminal,peptide
R4349 T6966 T6965 punct -,terminal
R4350 T6967 T6961 amod alone,peptide
R4351 T6968 T6969 punct (,391
R4352 T6969 T6959 parataxis 391,contains
R4353 T6970 T6971 punct –,422
R4354 T6971 T6969 prep 422,391
R4355 T6972 T6969 punct ),391
R4356 T6973 T6959 punct .,contains
R4357 T6975 T6976 nsubj Primers,were
R4358 T6977 T6978 nmod ST004,TT
R4359 T6978 T6976 attr TT,were
R4360 T6979 T6980 punct (,F
R4361 T6980 T6978 parataxis F,TT
R4362 T6981 T6980 punct ),F
R4363 T6982 T6978 nummod 5,TT
R4364 T6983 T6982 punct ',5
R4365 T6984 T6978 punct -,TT
R4366 T6985 T6978 compound AAA,TT
R4367 T6986 T6978 compound AGT,TT
R4368 T6987 T6978 compound CGA,TT
R4369 T6988 T6978 compound CTA,TT
R4370 T6989 T6978 compound CCA,TT
R4371 T6990 T6978 compound CTT,TT
R4372 T6991 T6978 compound CAA,TT
R4373 T6992 T6978 compound CAG,TT
R4374 T6993 T6978 compound GAC,TT
R4375 T6994 T6978 compound TCA,TT
R4376 T6995 T6978 punct -,TT
R4377 T6996 T6978 nummod 3,TT
R4378 T6997 T6978 punct ',TT
R4379 T6998 T6978 cc and,TT
R4380 T6999 T6978 conj ST005,TT
R4381 T7000 T7001 punct (,R
R4382 T7001 T6999 parataxis R,ST005
R4383 T7002 T7001 punct ),R
R4384 T7003 T6976 punct .,were
R4385 T7005 T7006 compound PAX6PST,CT
R4386 T7006 T7008 nsubj CT,contains
R4387 T7007 T7006 punct -,CT
R4388 T7009 T7010 det the,domain
R4389 T7010 T7008 dobj domain,contains
R4390 T7011 T7010 compound PST,domain
R4391 T7012 T7010 cc minus,domain
R4392 T7013 T7014 det the,peptide
R4393 T7014 T7010 conj peptide,domain
R4394 T7015 T7016 npadvmod C,terminal
R4395 T7016 T7014 amod terminal,peptide
R4396 T7017 T7016 punct -,terminal
R4397 T7018 T7019 punct (,278
R4398 T7019 T7008 parataxis 278,contains
R4399 T7020 T7021 punct –,390
R4400 T7021 T7019 prep 390,278
R4401 T7022 T7019 punct ),278
R4402 T7023 T7008 punct .,contains
R4403 T7025 T7026 nsubjpass This,made
R4404 T7027 T7026 auxpass was,made
R4405 T7028 T7026 prep by,made
R4406 T7029 T7028 pcomp cutting,by
R4407 T7030 T7029 dobj PAX6PST,cutting
R4408 T7031 T7029 prep with,cutting
R4409 T7032 T7031 pobj NdeI,with
R4410 T7033 T7032 cc and,NdeI
R4411 T7034 T7032 conj NotI,NdeI
R4412 T7035 T7036 aux to,drop
R4413 T7036 T7029 advcl drop,cutting
R4414 T7037 T7036 prt out,drop
R4415 T7038 T7039 det the,peptide
R4416 T7039 T7036 dobj peptide,drop
R4417 T7040 T7041 npadvmod C,terminal
R4418 T7041 T7039 amod terminal,peptide
R4419 T7042 T7041 punct -,terminal
R4420 T7043 T7029 punct ", ",cutting
R4421 T7044 T7029 cc and,cutting
R4422 T7045 T7029 conj inserting,cutting
R4423 T7046 T7047 det a,linker
R4424 T7047 T7045 dobj linker,inserting
R4425 T7048 T7047 amod synthetic,linker
R4426 T7049 T7047 prep between,linker
R4427 T7050 T7051 det the,sites
R4428 T7051 T7049 pobj sites,between
R4429 T7052 T7051 nummod two,sites
R4430 T7053 T7051 compound restriction,sites
R4431 T7054 T7026 punct .,made
R4432 T7056 T7057 det All,fragments
R4433 T7057 T7058 nsubjpass fragments,sequenced
R4434 T7059 T7057 acl generated,fragments
R4435 T7060 T7059 prep by,generated
R4436 T7061 T7060 pobj PCR,by
R4437 T7062 T7060 cc or,by
R4438 T7063 T7060 conj with,by
R4439 T7064 T7063 pobj linkers,with
R4440 T7065 T7058 auxpass were,sequenced
R4441 T7066 T7067 aux to,check
R4442 T7067 T7058 advcl check,sequenced
R4443 T7068 T7069 mark that,introduced
R4444 T7069 T7067 ccomp introduced,check
R4445 T7070 T7071 det no,errors
R4446 T7071 T7069 nsubjpass errors,introduced
R4447 T7072 T7069 aux had,introduced
R4448 T7073 T7069 auxpass been,introduced
R4449 T7074 T7058 punct .,sequenced
R4450 T7076 T7077 det A,construct
R4451 T7077 T7081 nsubjpass construct,generated
R4452 T7078 T7079 compound PAX6,domain
R4453 T7079 T7077 compound domain,construct
R4454 T7080 T7079 compound PST,domain
R4455 T7082 T7077 acl containing,construct
R4456 T7083 T7084 det the,mutation
R4457 T7084 T7082 dobj mutation,containing
R4458 T7085 T7084 appos 1627A,mutation
R4459 T7086 T7087 punct >,G
R4460 T7087 T7085 prep G,1627A
R4461 T7088 T7084 punct (,mutation
R4462 T7089 T7084 appos Q422R,mutation
R4463 T7090 T7084 punct ),mutation
R4464 T7091 T7081 auxpass was,generated
R4465 T7092 T7081 prep in,generated
R4466 T7093 T7094 det the,way
R4467 T7094 T7092 pobj way,in
R4468 T7095 T7094 amod same,way
R4469 T7096 T7094 prep as,way
R4470 T7097 T7098 det the,construct
R4471 T7098 T7096 pobj construct,as
R4472 T7099 T7098 compound PAX6PST,construct
R4473 T7100 T7092 punct ", ",in
R4474 T7101 T7092 cc but,in
R4475 T7102 T7092 conj using,in
R4476 T7103 T7104 det the,primer
R4477 T7104 T7102 dobj primer,using
R4478 T7105 T7104 amod reverse,primer
R4479 T7106 T7104 compound PCR,primer
R4480 T7107 T7108 nmod ST006,AG
R4481 T7108 T7104 appos AG,primer
R4482 T7109 T7108 nummod 5,AG
R4483 T7110 T7109 punct ',5
R4484 T7111 T7108 punct -,AG
R4485 T7112 T7108 compound TTT,AG
R4486 T7113 T7108 compound TGC,AG
R4487 T7114 T7108 compound GGC,AG
R4488 T7115 T7108 compound CGC,AG
R4489 T7116 T7108 compound TTT,AG
R4490 T7117 T7108 compound TTA,AG
R4491 T7118 T7108 compound CCG,AG
R4492 T7119 T7108 compound TAA,AG
R4493 T7120 T7108 compound TCT,AG
R4494 T7121 T7108 compound TGG,AG
R4495 T7122 T7108 compound CCA,AG
R4496 T7123 T7108 compound GTA,AG
R4497 T7124 T7108 compound TTG,AG
R4498 T7125 T7108 punct -,AG
R4499 T7126 T7108 nummod 3,AG
R4500 T7127 T7108 punct ',AG
R4501 T7128 T7104 punct ", ",primer
R4502 T7129 T7130 dep which,contains
R4503 T7130 T7104 relcl contains,primer
R4504 T7131 T7132 det the,substitution
R4505 T7132 T7130 dobj substitution,contains
R4506 T7133 T7132 compound mutant,substitution
R4507 T7134 T7132 compound nucleotide,substitution
R4508 T7135 T7136 punct (,underlined
R4509 T7136 T7104 parataxis underlined,primer
R4510 T7137 T7136 punct ),underlined
R4511 T7138 T7081 punct .,generated
R4512 T7140 T7141 compound cDNA,sequences
R4513 T7141 T7142 nsubjpass sequences,generated
R4514 T7143 T7141 acl containing,sequences
R4515 T7144 T7145 det the,mutations
R4516 T7145 T7143 dobj mutations,containing
R4517 T7146 T7145 appos 1615del10,mutations
R4518 T7147 T7148 punct [,28
R4519 T7148 T7146 parataxis 28,1615del10
R4520 T7149 T7148 punct ],28
R4521 T7150 T7146 cc and,1615del10
R4522 T7151 T7146 conj 1629insT,1615del10
R4523 T7152 T7151 punct (,1629insT
R4524 T7153 T7151 appos X423L,1629insT
R4525 T7154 T7151 punct ),1629insT
R4526 T7155 T7156 punct [,12
R4527 T7156 T7151 parataxis 12,1629insT
R4528 T7157 T7156 punct ],12
R4529 T7158 T7142 auxpass were,generated
R4530 T7159 T7142 prep by,generated
R4531 T7160 T7159 pobj PCR,by
R4532 T7161 T7160 prep from,PCR
R4533 T7162 T7163 amod reverse,transcribed
R4534 T7163 T7164 amod transcribed,RNA
R4535 T7164 T7161 pobj RNA,from
R4536 T7165 T7166 punct (,gift
R4537 T7166 T7164 parataxis gift,RNA
R4538 T7167 T7166 det a,gift
R4539 T7168 T7166 prep from,gift
R4540 T7169 T7170 compound Dr,Williamson
R4541 T7170 T7168 pobj Williamson,from
R4542 T7171 T7170 compound K,Williamson
R4543 T7172 T7170 cc and,Williamson
R4544 T7173 T7174 compound Prof,Heyningen
R4545 T7174 T7170 conj Heyningen,Williamson
R4546 T7175 T7174 compound V,Heyningen
R4547 T7176 T7174 compound van,Heyningen
R4548 T7177 T7166 punct ),gift
R4549 T7178 T7142 punct .,generated
R4550 T7180 T7181 nsubj Primers,were
R4551 T7182 T7183 nmod ST015,C
R4552 T7183 T7181 attr C,were
R4553 T7184 T7185 punct (,F
R4554 T7185 T7183 parataxis F,C
R4555 T7186 T7185 punct ),F
R4556 T7187 T7183 nummod 5,C
R4557 T7188 T7187 punct ',5
R4558 T7189 T7183 punct -,C
R4559 T7190 T7183 compound CCC,C
R4560 T7191 T7183 compound ACA,C
R4561 T7192 T7183 compound TAT,C
R4562 T7193 T7183 compound GCA,C
R4563 T7194 T7183 compound GAC,C
R4564 T7195 T7183 compound ACA,C
R4565 T7196 T7183 punct -,C
R4566 T7197 T7183 nummod 3,C
R4567 T7198 T7183 punct ',C
R4568 T7199 T7183 cc and,C
R4569 T7200 T7201 nmod ST031,TC
R4570 T7201 T7183 conj TC,C
R4571 T7202 T7203 punct (,R
R4572 T7203 T7201 parataxis R,TC
R4573 T7204 T7203 punct ),R
R4574 T7205 T7201 nummod 5,TC
R4575 T7206 T7205 punct ',5
R4576 T7207 T7201 punct -,TC
R4577 T7208 T7201 compound TTG,TC
R4578 T7209 T7201 compound CGG,TC
R4579 T7210 T7201 compound CCG,TC
R4580 T7211 T7201 compound CAT,TC
R4581 T7212 T7201 compound CCA,TC
R4582 T7213 T7201 compound TCC,TC
R4583 T7214 T7201 compound AGT,TC
R4584 T7215 T7201 compound CTA,TC
R4585 T7216 T7201 compound CAT,TC
R4586 T7217 T7201 compound TGT,TC
R4587 T7218 T7201 punct -,TC
R4588 T7219 T7201 nummod 3,TC
R4589 T7220 T7201 punct ',TC
R4590 T7221 T7181 punct .,were
R4591 T7223 T7224 det The,construct
R4592 T7224 T7226 nsubjpass construct,cut
R4593 T7225 T7224 compound PAX6PST,construct
R4594 T7227 T7226 auxpass was,cut
R4595 T7228 T7226 prep with,cut
R4596 T7229 T7228 pobj NdeI,with
R4597 T7230 T7229 cc and,NdeI
R4598 T7231 T7229 conj NotI,NdeI
R4599 T7232 T7233 aux to,release
R4600 T7233 T7226 advcl release,cut
R4601 T7234 T7235 det the,peptide
R4602 T7235 T7233 dobj peptide,release
R4603 T7236 T7235 amod normal,peptide
R4604 T7237 T7238 npadvmod C,terminal
R4605 T7238 T7235 amod terminal,peptide
R4606 T7239 T7238 punct -,terminal
R4607 T7240 T7226 punct ", ",cut
R4608 T7241 T7226 cc and,cut
R4609 T7242 T7243 det the,sequence
R4610 T7243 T7245 nsubjpass sequence,inserted
R4611 T7244 T7243 compound mutant,sequence
R4612 T7245 T7226 conj inserted,cut
R4613 T7246 T7245 auxpass was,inserted
R4614 T7247 T7245 punct .,inserted

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T6807 1874-1882 SO_EXT:sequence_insertion_process denotes inserted
T6806 1861-1869 SO_EXT:biological_sequence denotes sequence
T6805 1854-1860 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6804 1837-1844 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T6803 1826-1836 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T6802 1807-1814 GO_EXT:biological_release denotes release
T6801 1781-1784 MOP:0000780 denotes cut
T6800 1767-1776 SO_EXT:engineered_biological_sequence denotes construct
T6799 1702-1703 SO:0001031 denotes R
T6798 1656-1657 SO:0001030 denotes F
T6797 1636-1643 SO_EXT:0000112 denotes Primers
T6796 1576-1579 CHEBI_SO_EXT:RNA denotes RNA
T6795 1556-1575 GO:0001171 denotes reverse transcribed
T6794 1478-1487 SO_EXT:sequence_alteration_entity_or_process denotes mutations
T6793 1453-1462 SO_EXT:biological_sequence denotes sequences
T6792 1448-1452 SO_EXT:cDNA denotes cDNA
T6791 1421-1433 SO_EXT:sequence_substitution_entity_or_process denotes substitution
T6790 1410-1420 CHEBI_SO_EXT:nucleotide denotes nucleotide
T6789 1403-1409 SO_EXT:sequence_altered_entity_or_alteration_process denotes mutant
T6788 1309-1315 SO_EXT:0000132 denotes primer
T6787 1297-1304 _FRAGMENT denotes reverse
T6786 1272-1281 SO_EXT:engineered_biological_sequence denotes construct
T6785 1217-1218 CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group denotes G
T6784 1202-1210 SO_EXT:sequence_alteration_entity_or_process denotes mutation
T6783 1177-1186 SO_EXT:engineered_biological_sequence denotes construct
T6782 1170-1176 SO_EXT:0000417 denotes domain
T6781 1161-1165 PR_EXT:000012318 denotes PAX6
T6780 1033-1050 SO_EXT:0000061 denotes restriction sites
T6779 988-997 SO_EXT:sequence_insertion_process denotes inserting
T6778 975-982 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T6777 964-974 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T6776 913-920 MOP:0000780 denotes cutting
T6775 877-884 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T6774 866-876 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T6773 849-855 SO_EXT:0000417 denotes domain
T6772 817-818 SO:0001031 denotes R
T6771 754-755 SO:0001030 denotes F
T6770 734-741 SO_EXT:0000112 denotes Primers
T6769 709-716 CHEBI_SO_EXT:peptide_or_peptide_region denotes peptide
T6768 698-708 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminal
T6767 605-606 SO:0001031 denotes R
T6766 537-544 SO:0001030 denotes forward
T6765 517-524 SO_EXT:0000112 denotes Primers
T6764 507-514 CHEBI_PR_EXT:protein denotes protein
T6763 502-506 PR_EXT:000012318 denotes PAX6
T6762 463-474 CHEBI_SO_EXT:amino_acid denotes amino acids
T6761 455-461 SO_EXT:0000417 denotes domain
T6760 416-422 SO_EXT:0000417 denotes domain
T6759 408-415 CHEMINF_GO_EXT:chemical_binding_or_bond_formation denotes binding
T6758 404-407 CHEBI_SO_EXT:DNA denotes DNA
T6757 399-403 PR_EXT:P04386 denotes GAL4
T6756 393-398 NCBITaxon_EXT:yeast denotes yeast
T6755 380-385 SO_EXT:sequence_fusion_process denotes fused
T6754 372-379 CHEBI_PR_EXT:protein denotes protein
T6753 305-311 SO_EXT:0000440 denotes vector
T6752 294-304 GO:0010467 denotes expression
T6751 269-279 SO_EXT:engineered_biological_sequence denotes constructs
T6750 264-268 SO_EXT:cDNA denotes cDNA
T6749 259-263 PR_EXT:000012318 denotes PAX6
T6748 164-171 SO_EXT:sequence_variant_or_variation denotes Variant
T6747 156-163 SO_EXT:0001023 denotes Allelic
T6746 151-155 PR_EXT:000012318 denotes PAX6
T6745 145-150 NCBITaxon:9606 denotes Human
T6744 116-125 SO_EXT:biological_sequence denotes sequences
T6743 98-105 CHEBI_PR_EXT:protein denotes protein
T6742 89-93 SO_EXT:cDNA denotes cDNA
T6741 84-88 PR_EXT:000012318 denotes PAX6
T6740 78-83 NCBITaxon:9606 denotes human
T6739 41-51 CHEBI_SO_EXT:amino_acid denotes amino acid
T6738 32-36 SO_EXT:cDNA denotes cDNA
T6737 17-27 SO_EXT:engineered_biological_sequence denotes constructs
T6736 0-5 NCBITaxon_EXT:yeast denotes Yeast
R4201 T6788 T6787 _lexicallyChainedTo primer,reverse

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T6719 537-544 SO:0001030 denotes forward
T6718 517-524 SO:0000112 denotes Primers
T6717 502-506 PR:000012318 denotes PAX6
T6716 455-461 SO:0000417 denotes domain
T6715 416-422 SO:0000417 denotes domain
T6714 399-403 PR:P04386 denotes GAL4
T6713 305-311 SO:0000440 denotes vector
T6712 294-304 GO:0010467 denotes expression
T6711 259-263 PR:000012318 denotes PAX6
T6710 156-163 SO:0001023 denotes Allelic
T6709 151-155 PR:000012318 denotes PAX6
T6708 145-150 NCBITaxon:9606 denotes Human
T6707 84-88 PR:000012318 denotes PAX6
T6706 78-83 NCBITaxon:9606 denotes human
T6735 1781-1784 MOP:0000780 denotes cut
T6734 1702-1703 SO:0001031 denotes R
T6733 1656-1657 SO:0001030 denotes F
T6732 1636-1643 SO:0000112 denotes Primers
T6731 1556-1575 GO:0001171 denotes reverse transcribed
T6730 1309-1315 SO:0000132 denotes primer
T6729 1297-1304 _FRAGMENT denotes reverse
T6728 1170-1176 SO:0000417 denotes domain
T6727 1161-1165 PR:000012318 denotes PAX6
T6726 1033-1050 SO:0000061 denotes restriction sites
T6725 913-920 MOP:0000780 denotes cutting
T6724 849-855 SO:0000417 denotes domain
T6723 817-818 SO:0001031 denotes R
T6722 754-755 SO:0001030 denotes F
T6721 734-741 SO:0000112 denotes Primers
T6720 605-606 SO:0001031 denotes R
R4200 T6730 T6729 _lexicallyChainedTo primer,reverse