PMC:1208879 / 25700-27583 JSONTXT

{"project":"craft-sa-dev","denotations":[{"id":"T7133","span":{"begin":1403,"end":1409},"obj":"NN"},{"id":"T7132","span":{"begin":1421,"end":1433},"obj":"NN"},{"id":"T7131","span":{"begin":1399,"end":1402},"obj":"DT"},{"id":"T7130","span":{"begin":1390,"end":1398},"obj":"VBZ"},{"id":"T7129","span":{"begin":1384,"end":1389},"obj":"WDT"},{"id":"T7128","span":{"begin":1382,"end":1384},"obj":","},{"id":"T7127","span":{"begin":1381,"end":1382},"obj":"SYM"},{"id":"T7126","span":{"begin":1380,"end":1381},"obj":"CD"},{"id":"T7125","span":{"begin":1379,"end":1380},"obj":"HYPH"},{"id":"T7124","span":{"begin":1373,"end":1376},"obj":"NN"},{"id":"T7123","span":{"begin":1369,"end":1372},"obj":"NN"},{"id":"T7122","span":{"begin":1365,"end":1368},"obj":"NN"},{"id":"T7121","span":{"begin":1361,"end":1364},"obj":"NN"},{"id":"T7120","span":{"begin":1357,"end":1360},"obj":"NN"},{"id":"T7119","span":{"begin":1353,"end":1356},"obj":"NN"},{"id":"T7118","span":{"begin":1349,"end":1352},"obj":"NN"},{"id":"T7117","span":{"begin":1345,"end":1348},"obj":"NN"},{"id":"T7116","span":{"begin":1341,"end":1344},"obj":"NN"},{"id":"T7115","span":{"begin":1337,"end":1340},"obj":"NN"},{"id":"T7114","span":{"begin":1333,"end":1336},"obj":"NN"},{"id":"T7113","span":{"begin":1329,"end":1332},"obj":"NN"},{"id":"T7112","span":{"begin":1325,"end":1328},"obj":"NN"},{"id":"T7111","span":{"begin":1324,"end":1325},"obj":"HYPH"},{"id":"T7110","span":{"begin":1323,"end":1324},"obj":"SYM"},{"id":"T7109","span":{"begin":1322,"end":1323},"obj":"CD"},{"id":"T7108","span":{"begin":1377,"end":1379},"obj":"NN"},{"id":"T7107","span":{"begin":1316,"end":1321},"obj":"NN"},{"id":"T7106","span":{"begin":1305,"end":1308},"obj":"NN"},{"id":"T7105","span":{"begin":1297,"end":1304},"obj":"JJ"},{"id":"T7104","span":{"begin":1309,"end":1315},"obj":"NN"},{"id":"T7103","span":{"begin":1293,"end":1296},"obj":"DT"},{"id":"T7102","span":{"begin":1287,"end":1292},"obj":"VBG"},{"id":"T7101","span":{"begin":1283,"end":1286},"obj":"CC"},{"id":"T7100","span":{"begin":1281,"end":1283},"obj":","},{"id":"T7099","span":{"begin":1264,"end":1271},"obj":"NN"},{"id":"T7098","span":{"begin":1272,"end":1281},"obj":"NN"},{"id":"T7097","span":{"begin":1260,"end":1263},"obj":"DT"},{"id":"T7096","span":{"begin":1257,"end":1259},"obj":"IN"},{"id":"T7095","span":{"begin":1248,"end":1252},"obj":"JJ"},{"id":"T7094","span":{"begin":1253,"end":1256},"obj":"NN"},{"id":"T7093","span":{"begin":1244,"end":1247},"obj":"DT"},{"id":"T7092","span":{"begin":1241,"end":1243},"obj":"IN"},{"id":"T7091","span":{"begin":1227,"end":1230},"obj":"VBD"},{"id":"T7090","span":{"begin":1225,"end":1226},"obj":"-RRB-"},{"id":"T7089","span":{"begin":1220,"end":1225},"obj":"NN"},{"id":"T7088","span":{"begin":1219,"end":1220},"obj":"-LRB-"},{"id":"T7087","span":{"begin":1217,"end":1218},"obj":"NN"},{"id":"T7086","span":{"begin":1216,"end":1217},"obj":"SYM"},{"id":"T7085","span":{"begin":1211,"end":1216},"obj":"NN"},{"id":"T7084","span":{"begin":1202,"end":1210},"obj":"NN"},{"id":"T7083","span":{"begin":1198,"end":1201},"obj":"DT"},{"id":"T7082","span":{"begin":1187,"end":1197},"obj":"VBG"},{"id":"T7081","span":{"begin":1231,"end":1240},"obj":"VBN"},{"id":"T7080","span":{"begin":1166,"end":1169},"obj":"NN"},{"id":"T7079","span":{"begin":1170,"end":1176},"obj":"NN"},{"id":"T7078","span":{"begin":1161,"end":1165},"obj":"NN"},{"id":"T7077","span":{"begin":1177,"end":1186},"obj":"NN"},{"id":"T7076","span":{"begin":1159,"end":1160},"obj":"DT"},{"id":"T7075","span":{"begin":1158,"end":1447},"obj":"sentence"},{"id":"T7074","span":{"begin":1157,"end":1158},"obj":"."},{"id":"T7073","span":{"begin":1142,"end":1146},"obj":"VBN"},{"id":"T7072","span":{"begin":1138,"end":1141},"obj":"VBD"},{"id":"T7071","span":{"begin":1131,"end":1137},"obj":"NNS"},{"id":"T7070","span":{"begin":1128,"end":1130},"obj":"DT"},{"id":"T7069","span":{"begin":1147,"end":1157},"obj":"VBN"},{"id":"T7068","span":{"begin":1123,"end":1127},"obj":"IN"},{"id":"T7067","span":{"begin":1117,"end":1122},"obj":"VB"},{"id":"T7066","span":{"begin":1114,"end":1116},"obj":"TO"},{"id":"T7065","span":{"begin":1099,"end":1103},"obj":"VBD"},{"id":"T7064","span":{"begin":1091,"end":1098},"obj":"NNS"},{"id":"T7063","span":{"begin":1086,"end":1090},"obj":"IN"},{"id":"T7062","span":{"begin":1083,"end":1085},"obj":"CC"},{"id":"T7061","span":{"begin":1079,"end":1082},"obj":"NN"},{"id":"T7060","span":{"begin":1076,"end":1078},"obj":"IN"},{"id":"T7059","span":{"begin":1066,"end":1075},"obj":"VBN"},{"id":"T7058","span":{"begin":1104,"end":1113},"obj":"VBN"},{"id":"T7057","span":{"begin":1056,"end":1065},"obj":"NNS"},{"id":"T7056","span":{"begin":1052,"end":1055},"obj":"DT"},{"id":"T7055","span":{"begin":1051,"end":1158},"obj":"sentence"},{"id":"T7054","span":{"begin":1050,"end":1051},"obj":"."},{"id":"T7053","span":{"begin":1033,"end":1044},"obj":"NN"},{"id":"T7052","span":{"begin":1029,"end":1032},"obj":"CD"},{"id":"T7051","span":{"begin":1045,"end":1050},"obj":"NNS"},{"id":"T7050","span":{"begin":1025,"end":1028},"obj":"DT"},{"id":"T7049","span":{"begin":1017,"end":1024},"obj":"IN"},{"id":"T7048","span":{"begin":1000,"end":1009},"obj":"JJ"},{"id":"T7047","span":{"begin":1010,"end":1016},"obj":"NN"},{"id":"T7046","span":{"begin":998,"end":999},"obj":"DT"},{"id":"T7045","span":{"begin":988,"end":997},"obj":"VBG"},{"id":"T7044","span":{"begin":984,"end":987},"obj":"CC"},{"id":"T7043","span":{"begin":982,"end":984},"obj":","},{"id":"T7042","span":{"begin":965,"end":966},"obj":"HYPH"},{"id":"T7041","span":{"begin":966,"end":974},"obj":"JJ"},{"id":"T7040","span":{"begin":964,"end":965},"obj":"NN"},{"id":"T7039","span":{"begin":975,"end":982},"obj":"NN"},{"id":"T7038","span":{"begin":960,"end":963},"obj":"DT"},{"id":"T7037","span":{"begin":956,"end":959},"obj":"RP"},{"id":"T7036","span":{"begin":951,"end":955},"obj":"VB"},{"id":"T7035","span":{"begin":948,"end":950},"obj":"TO"},{"id":"T7034","span":{"begin":943,"end":947},"obj":"NN"},{"id":"T7033","span":{"begin":939,"end":942},"obj":"CC"},{"id":"T7032","span":{"begin":934,"end":938},"obj":"NN"},{"id":"T7031","span":{"begin":929,"end":933},"obj":"IN"},{"id":"T7030","span":{"begin":921,"end":928},"obj":"NN"},{"id":"T7029","span":{"begin":913,"end":920},"obj":"VBG"},{"id":"T7028","span":{"begin":910,"end":912},"obj":"IN"},{"id":"T7027","span":{"begin":901,"end":904},"obj":"VBD"},{"id":"T7026","span":{"begin":905,"end":909},"obj":"VBN"},{"id":"T7025","span":{"begin":896,"end":900},"obj":"DT"},{"id":"T7024","span":{"begin":895,"end":1051},"obj":"sentence"},{"id":"T7023","span":{"begin":894,"end":895},"obj":"."},{"id":"T7022","span":{"begin":893,"end":894},"obj":"-RRB-"},{"id":"T7021","span":{"begin":890,"end":893},"obj":"CD"},{"id":"T7020","span":{"begin":889,"end":890},"obj":"SYM"},{"id":"T7019","span":{"begin":886,"end":889},"obj":"CD"},{"id":"T7018","span":{"begin":885,"end":886},"obj":"-LRB-"},{"id":"T7017","span":{"begin":867,"end":868},"obj":"HYPH"},{"id":"T7016","span":{"begin":868,"end":876},"obj":"JJ"},{"id":"T7015","span":{"begin":866,"end":867},"obj":"NN"},{"id":"T7014","span":{"begin":877,"end":884},"obj":"NN"},{"id":"T7013","span":{"begin":862,"end":865},"obj":"DT"},{"id":"T7012","span":{"begin":856,"end":861},"obj":"CC"},{"id":"T7011","span":{"begin":845,"end":848},"obj":"NN"},{"id":"T7010","span":{"begin":849,"end":855},"obj":"NN"},{"id":"T7009","span":{"begin":841,"end":844},"obj":"DT"},{"id":"T7008","span":{"begin":832,"end":840},"obj":"VBZ"},{"id":"T7007","span":{"begin":828,"end":829},"obj":"HYPH"},{"id":"T7006","span":{"begin":829,"end":831},"obj":"NN"},{"id":"T7005","span":{"begin":821,"end":828},"obj":"NN"},{"id":"T7004","span":{"begin":820,"end":895},"obj":"sentence"},{"id":"T7003","span":{"begin":819,"end":820},"obj":"."},{"id":"T7002","span":{"begin":818,"end":819},"obj":"-RRB-"},{"id":"T7001","span":{"begin":817,"end":818},"obj":"NN"},{"id":"T7000","span":{"begin":816,"end":817},"obj":"-LRB-"},{"id":"T6999","span":{"begin":810,"end":815},"obj":"NN"},{"id":"T6998","span":{"begin":806,"end":809},"obj":"CC"},{"id":"T6997","span":{"begin":804,"end":805},"obj":"SYM"},{"id":"T6996","span":{"begin":803,"end":804},"obj":"CD"},{"id":"T6995","span":{"begin":802,"end":803},"obj":"HYPH"},{"id":"T6994","span":{"begin":796,"end":799},"obj":"NN"},{"id":"T6993","span":{"begin":792,"end":795},"obj":"NN"},{"id":"T6992","span":{"begin":788,"end":791},"obj":"NN"},{"id":"T6991","span":{"begin":784,"end":787},"obj":"NN"},{"id":"T6990","span":{"begin":780,"end":783},"obj":"NN"},{"id":"T6989","span":{"begin":776,"end":779},"obj":"NN"},{"id":"T6988","span":{"begin":772,"end":775},"obj":"NN"},{"id":"T6987","span":{"begin":768,"end":771},"obj":"NN"},{"id":"T6986","span":{"begin":764,"end":767},"obj":"NN"},{"id":"T6985","span":{"begin":760,"end":763},"obj":"NN"},{"id":"T6984","span":{"begin":759,"end":760},"obj":"HYPH"},{"id":"T6983","span":{"begin":758,"end":759},"obj":"SYM"},{"id":"T6982","span":{"begin":757,"end":758},"obj":"CD"},{"id":"T6981","span":{"begin":755,"end":756},"obj":"-RRB-"},{"id":"T6980","span":{"begin":754,"end":755},"obj":"NN"},{"id":"T6979","span":{"begin":753,"end":754},"obj":"-LRB-"},{"id":"T6978","span":{"begin":800,"end":802},"obj":"NN"},{"id":"T6977","span":{"begin":747,"end":752},"obj":"NN"},{"id":"T6976","span":{"begin":742,"end":746},"obj":"VBD"},{"id":"T6975","span":{"begin":734,"end":741},"obj":"NNS"},{"id":"T6974","span":{"begin":733,"end":820},"obj":"sentence"},{"id":"T6973","span":{"begin":732,"end":733},"obj":"."},{"id":"T6972","span":{"begin":731,"end":732},"obj":"-RRB-"},{"id":"T6971","span":{"begin":728,"end":731},"obj":"CD"},{"id":"T6970","span":{"begin":727,"end":728},"obj":"SYM"},{"id":"T6969","span":{"begin":724,"end":727},"obj":"CD"},{"id":"T6968","span":{"begin":723,"end":724},"obj":"-LRB-"},{"id":"T6967","span":{"begin":717,"end":722},"obj":"JJ"},{"id":"T6966","span":{"begin":699,"end":700},"obj":"HYPH"},{"id":"T6965","span":{"begin":700,"end":708},"obj":"JJ"},{"id":"T6964","span":{"begin":698,"end":699},"obj":"NN"},{"id":"T6963","span":{"begin":690,"end":697},"obj":"VBN"},{"id":"T6962","span":{"begin":684,"end":689},"obj":"RB"},{"id":"T6961","span":{"begin":709,"end":716},"obj":"NN"},{"id":"T6960","span":{"begin":680,"end":683},"obj":"DT"},{"id":"T6959","span":{"begin":671,"end":679},"obj":"VBZ"},{"id":"T6958","span":{"begin":663,"end":670},"obj":"NN"},{"id":"T6957","span":{"begin":662,"end":733},"obj":"sentence"},{"id":"T6956","span":{"begin":661,"end":662},"obj":"."},{"id":"T6955","span":{"begin":660,"end":661},"obj":"SYM"},{"id":"T6954","span":{"begin":659,"end":660},"obj":"CD"},{"id":"T6953","span":{"begin":658,"end":659},"obj":"HYPH"},{"id":"T6952","span":{"begin":651,"end":654},"obj":"NN"},{"id":"T6951","span":{"begin":647,"end":650},"obj":"NN"},{"id":"T6950","span":{"begin":643,"end":646},"obj":"NN"},{"id":"T6949","span":{"begin":639,"end":642},"obj":"NN"},{"id":"T6948","span":{"begin":635,"end":638},"obj":"NN"},{"id":"T6947","span":{"begin":631,"end":634},"obj":"NN"},{"id":"T6946","span":{"begin":627,"end":630},"obj":"NN"},{"id":"T6945","span":{"begin":623,"end":626},"obj":"NN"},{"id":"T6944","span":{"begin":619,"end":622},"obj":"NN"},{"id":"T6943","span":{"begin":615,"end":618},"obj":"NN"},{"id":"T6942","span":{"begin":611,"end":614},"obj":"NN"},{"id":"T6941","span":{"begin":610,"end":611},"obj":"HYPH"},{"id":"T6940","span":{"begin":609,"end":610},"obj":"SYM"},{"id":"T6939","span":{"begin":608,"end":609},"obj":"CD"},{"id":"T6938","span":{"begin":606,"end":607},"obj":"-RRB-"},{"id":"T6937","span":{"begin":605,"end":606},"obj":"NN"},{"id":"T6936","span":{"begin":604,"end":605},"obj":"-LRB-"},{"id":"T6935","span":{"begin":655,"end":658},"obj":"NN"},{"id":"T6934","span":{"begin":598,"end":603},"obj":"NN"},{"id":"T6933","span":{"begin":594,"end":597},"obj":"CC"},{"id":"T6932","span":{"begin":592,"end":593},"obj":"SYM"},{"id":"T6931","span":{"begin":591,"end":592},"obj":"CD"},{"id":"T6930","span":{"begin":590,"end":591},"obj":"HYPH"},{"id":"T6928","span":{"begin":581,"end":584},"obj":"NN"},{"id":"T6927","span":{"begin":577,"end":580},"obj":"NN"},{"id":"T6926","span":{"begin":573,"end":576},"obj":"NN"},{"id":"T6925","span":{"begin":569,"end":572},"obj":"NN"},{"id":"T6924","span":{"begin":565,"end":568},"obj":"NN"},{"id":"T6923","span":{"begin":561,"end":564},"obj":"NN"},{"id":"T6922","span":{"begin":557,"end":560},"obj":"NN"},{"id":"T6921","span":{"begin":553,"end":556},"obj":"NN"},{"id":"T6920","span":{"begin":549,"end":552},"obj":"NN"},{"id":"T6919","span":{"begin":548,"end":549},"obj":"HYPH"},{"id":"T6918","span":{"begin":547,"end":548},"obj":"SYM"},{"id":"T6909","span":{"begin":516,"end":662},"obj":"sentence"},{"id":"T6908","span":{"begin":515,"end":516},"obj":"."},{"id":"T6907","span":{"begin":514,"end":515},"obj":"-RRB-"},{"id":"T6906","span":{"begin":502,"end":506},"obj":"NN"},{"id":"T6905","span":{"begin":494,"end":495},"obj":"HYPH"},{"id":"T6904","span":{"begin":495,"end":501},"obj":"NN"},{"id":"T6903","span":{"begin":490,"end":494},"obj":"JJ"},{"id":"T6902","span":{"begin":507,"end":514},"obj":"NN"},{"id":"T6901","span":{"begin":486,"end":489},"obj":"DT"},{"id":"T6900","span":{"begin":483,"end":485},"obj":"IN"},{"id":"T6899","span":{"begin":479,"end":482},"obj":"CD"},{"id":"T6898","span":{"begin":478,"end":479},"obj":"SYM"},{"id":"T6897","span":{"begin":469,"end":474},"obj":"NNS"},{"id":"T6896","span":{"begin":463,"end":468},"obj":"NN"},{"id":"T6895","span":{"begin":475,"end":478},"obj":"CD"},{"id":"T6894","span":{"begin":462,"end":463},"obj":"-LRB-"},{"id":"T6893","span":{"begin":451,"end":454},"obj":"NN"},{"id":"T6892","span":{"begin":445,"end":450},"obj":"JJ"},{"id":"T6891","span":{"begin":455,"end":461},"obj":"NN"},{"id":"T6890","span":{"begin":441,"end":444},"obj":"DT"},{"id":"T6889","span":{"begin":432,"end":440},"obj":"VBZ"},{"id":"T6888","span":{"begin":424,"end":431},"obj":"NN"},{"id":"T6887","span":{"begin":423,"end":516},"obj":"sentence"},{"id":"T6886","span":{"begin":422,"end":423},"obj":"."},{"id":"T6885","span":{"begin":408,"end":415},"obj":"NN"},{"id":"T6884","span":{"begin":404,"end":407},"obj":"NN"},{"id":"T6883","span":{"begin":399,"end":403},"obj":"NN"},{"id":"T6882","span":{"begin":393,"end":398},"obj":"NN"},{"id":"T6881","span":{"begin":416,"end":422},"obj":"NN"},{"id":"T6880","span":{"begin":389,"end":392},"obj":"DT"},{"id":"T6879","span":{"begin":386,"end":388},"obj":"IN"},{"id":"T6878","span":{"begin":380,"end":385},"obj":"VBN"},{"id":"T6877","span":{"begin":372,"end":379},"obj":"NN"},{"id":"T6876","span":{"begin":370,"end":371},"obj":"DT"},{"id":"T6875","span":{"begin":360,"end":369},"obj":"VBZ"},{"id":"T6874","span":{"begin":354,"end":359},"obj":"WDT"},{"id":"T6873","span":{"begin":352,"end":354},"obj":","},{"id":"T6872","span":{"begin":351,"end":352},"obj":"-RRB-"},{"id":"T6871","span":{"begin":341,"end":351},"obj":"NNP"},{"id":"T6870","span":{"begin":339,"end":341},"obj":","},{"id":"T6869","span":{"begin":325,"end":326},"obj":"HYPH"},{"id":"T6868","span":{"begin":326,"end":332},"obj":"NN"},{"id":"T6867","span":{"begin":322,"end":325},"obj":"CD"},{"id":"T6866","span":{"begin":313,"end":321},"obj":"NNP"},{"id":"T6865","span":{"begin":333,"end":339},"obj":"NN"},{"id":"T6864","span":{"begin":312,"end":313},"obj":"-LRB-"},{"id":"T6863","span":{"begin":294,"end":304},"obj":"NN"},{"id":"T6862","span":{"begin":287,"end":293},"obj":"NN"},{"id":"T6861","span":{"begin":305,"end":311},"obj":"NN"},{"id":"T6860","span":{"begin":283,"end":286},"obj":"DT"},{"id":"T6859","span":{"begin":280,"end":282},"obj":"IN"},{"id":"T6858","span":{"begin":264,"end":268},"obj":"NN"},{"id":"T6857","span":{"begin":259,"end":263},"obj":"NN"},{"id":"T6856","span":{"begin":269,"end":279},"obj":"NNS"},{"id":"T6855","span":{"begin":253,"end":258},"obj":"CD"},{"id":"T6854","span":{"begin":248,"end":252},"obj":"VB"},{"id":"T6853","span":{"begin":245,"end":247},"obj":"TO"},{"id":"T6852","span":{"begin":235,"end":239},"obj":"VBD"},{"id":"T6851","span":{"begin":240,"end":244},"obj":"VBN"},{"id":"T6850","span":{"begin":213,"end":223},"obj":"NN"},{"id":"T6849","span":{"begin":209,"end":212},"obj":"CC"},{"id":"T6848","span":{"begin":205,"end":208},"obj":"NN"},{"id":"T6847","span":{"begin":224,"end":234},"obj":"NNS"},{"id":"T6846","span":{"begin":196,"end":204},"obj":"JJ"},{"id":"T6845","span":{"begin":195,"end":423},"obj":"sentence"},{"id":"T6844","span":{"begin":194,"end":195},"obj":"."},{"id":"T6843","span":{"begin":193,"end":194},"obj":"-RRB-"},{"id":"T6842","span":{"begin":191,"end":193},"obj":"CD"},{"id":"T6841","span":{"begin":190,"end":191},"obj":"-LRB-"},{"id":"T6840","span":{"begin":181,"end":184},"obj":"NN"},{"id":"T6839","span":{"begin":164,"end":171},"obj":"NN"},{"id":"T6838","span":{"begin":156,"end":163},"obj":"JJ"},{"id":"T6837","span":{"begin":172,"end":180},"obj":"NN"},{"id":"T6836","span":{"begin":151,"end":155},"obj":"NN"},{"id":"T6835","span":{"begin":145,"end":150},"obj":"JJ"},{"id":"T6834","span":{"begin":185,"end":189},"obj":"NN"},{"id":"T6833","span":{"begin":141,"end":144},"obj":"DT"},{"id":"T6832","span":{"begin":136,"end":140},"obj":"IN"},{"id":"T6831","span":{"begin":126,"end":135},"obj":"JJ"},{"id":"T6830","span":{"begin":106,"end":115},"obj":"NN"},{"id":"T6829","span":{"begin":98,"end":105},"obj":"NN"},{"id":"T6828","span":{"begin":94,"end":97},"obj":"CC"},{"id":"T6827","span":{"begin":84,"end":88},"obj":"NN"},{"id":"T6826","span":{"begin":89,"end":93},"obj":"NN"},{"id":"T6825","span":{"begin":78,"end":83},"obj":"JJ"},{"id":"T6824","span":{"begin":116,"end":125},"obj":"NNS"},{"id":"T6823","span":{"begin":74,"end":77},"obj":"DT"},{"id":"T6822","span":{"begin":71,"end":73},"obj":"IN"},{"id":"T6821","span":{"begin":65,"end":70},"obj":"VBN"},{"id":"T6820","span":{"begin":62,"end":64},"obj":"VBZ"},{"id":"T6819","span":{"begin":47,"end":51},"obj":"NN"},{"id":"T6818","span":{"begin":41,"end":46},"obj":"NN"},{"id":"T6817","span":{"begin":37,"end":40},"obj":"CC"},{"id":"T6816","span":{"begin":32,"end":36},"obj":"NN"},{"id":"T6815","span":{"begin":52,"end":61},"obj":"NN"},{"id":"T6814","span":{"begin":28,"end":31},"obj":"DT"},{"id":"T6813","span":{"begin":27,"end":195},"obj":"sentence"},{"id":"T6812","span":{"begin":9,"end":10},"obj":"HYPH"},{"id":"T6811","span":{"begin":10,"end":16},"obj":"NN"},{"id":"T6810","span":{"begin":6,"end":9},"obj":"CD"},{"id":"T6809","span":{"begin":17,"end":27},"obj":"NNS"},{"id":"T6808","span":{"begin":0,"end":5},"obj":"NN"},{"id":"T7154","span":{"begin":1522,"end":1523},"obj":"-RRB-"},{"id":"T7247","span":{"begin":1882,"end":1883},"obj":"."},{"id":"T7246","span":{"begin":1870,"end":1873},"obj":"VBD"},{"id":"T7245","span":{"begin":1874,"end":1882},"obj":"VBN"},{"id":"T7244","span":{"begin":1854,"end":1860},"obj":"NN"},{"id":"T7243","span":{"begin":1861,"end":1869},"obj":"NN"},{"id":"T7242","span":{"begin":1850,"end":1853},"obj":"DT"},{"id":"T7241","span":{"begin":1846,"end":1849},"obj":"CC"},{"id":"T7240","span":{"begin":1844,"end":1846},"obj":","},{"id":"T7239","span":{"begin":1827,"end":1828},"obj":"HYPH"},{"id":"T7238","span":{"begin":1828,"end":1836},"obj":"JJ"},{"id":"T7237","span":{"begin":1826,"end":1827},"obj":"NN"},{"id":"T7236","span":{"begin":1819,"end":1825},"obj":"JJ"},{"id":"T7235","span":{"begin":1837,"end":1844},"obj":"NN"},{"id":"T7234","span":{"begin":1815,"end":1818},"obj":"DT"},{"id":"T7233","span":{"begin":1807,"end":1814},"obj":"VB"},{"id":"T7232","span":{"begin":1804,"end":1806},"obj":"TO"},{"id":"T7231","span":{"begin":1799,"end":1803},"obj":"NN"},{"id":"T7230","span":{"begin":1795,"end":1798},"obj":"CC"},{"id":"T7229","span":{"begin":1790,"end":1794},"obj":"NN"},{"id":"T7228","span":{"begin":1785,"end":1789},"obj":"IN"},{"id":"T7227","span":{"begin":1777,"end":1780},"obj":"VBD"},{"id":"T7226","span":{"begin":1781,"end":1784},"obj":"VBN"},{"id":"T7225","span":{"begin":1759,"end":1766},"obj":"NN"},{"id":"T7224","span":{"begin":1767,"end":1776},"obj":"NN"},{"id":"T7223","span":{"begin":1755,"end":1758},"obj":"DT"},{"id":"T7222","span":{"begin":1754,"end":1883},"obj":"sentence"},{"id":"T7221","span":{"begin":1753,"end":1754},"obj":"."},{"id":"T7220","span":{"begin":1752,"end":1753},"obj":"SYM"},{"id":"T7219","span":{"begin":1751,"end":1752},"obj":"CD"},{"id":"T7218","span":{"begin":1750,"end":1751},"obj":"HYPH"},{"id":"T7217","span":{"begin":1744,"end":1747},"obj":"NN"},{"id":"T7216","span":{"begin":1740,"end":1743},"obj":"NN"},{"id":"T7215","span":{"begin":1736,"end":1739},"obj":"NN"},{"id":"T7214","span":{"begin":1732,"end":1735},"obj":"NN"},{"id":"T7213","span":{"begin":1728,"end":1731},"obj":"NN"},{"id":"T7212","span":{"begin":1724,"end":1727},"obj":"NN"},{"id":"T7211","span":{"begin":1720,"end":1723},"obj":"NN"},{"id":"T7210","span":{"begin":1716,"end":1719},"obj":"NN"},{"id":"T7209","span":{"begin":1712,"end":1715},"obj":"NN"},{"id":"T7208","span":{"begin":1708,"end":1711},"obj":"NN"},{"id":"T7207","span":{"begin":1707,"end":1708},"obj":"HYPH"},{"id":"T7206","span":{"begin":1706,"end":1707},"obj":"SYM"},{"id":"T7205","span":{"begin":1705,"end":1706},"obj":"CD"},{"id":"T7204","span":{"begin":1703,"end":1704},"obj":"-RRB-"},{"id":"T7203","span":{"begin":1702,"end":1703},"obj":"NN"},{"id":"T7202","span":{"begin":1701,"end":1702},"obj":"-LRB-"},{"id":"T7201","span":{"begin":1748,"end":1750},"obj":"NN"},{"id":"T7200","span":{"begin":1695,"end":1700},"obj":"NN"},{"id":"T7199","span":{"begin":1691,"end":1694},"obj":"CC"},{"id":"T7198","span":{"begin":1689,"end":1690},"obj":"SYM"},{"id":"T7197","span":{"begin":1688,"end":1689},"obj":"CD"},{"id":"T7196","span":{"begin":1687,"end":1688},"obj":"HYPH"},{"id":"T7195","span":{"begin":1682,"end":1685},"obj":"NN"},{"id":"T7194","span":{"begin":1678,"end":1681},"obj":"NN"},{"id":"T7193","span":{"begin":1674,"end":1677},"obj":"NN"},{"id":"T7192","span":{"begin":1670,"end":1673},"obj":"NN"},{"id":"T7191","span":{"begin":1666,"end":1669},"obj":"NN"},{"id":"T7190","span":{"begin":1662,"end":1665},"obj":"NN"},{"id":"T7189","span":{"begin":1661,"end":1662},"obj":"HYPH"},{"id":"T7188","span":{"begin":1660,"end":1661},"obj":"SYM"},{"id":"T7187","span":{"begin":1659,"end":1660},"obj":"CD"},{"id":"T7186","span":{"begin":1657,"end":1658},"obj":"-RRB-"},{"id":"T7185","span":{"begin":1656,"end":1657},"obj":"NN"},{"id":"T7184","span":{"begin":1655,"end":1656},"obj":"-LRB-"},{"id":"T7183","span":{"begin":1686,"end":1687},"obj":"NN"},{"id":"T7182","span":{"begin":1649,"end":1654},"obj":"NN"},{"id":"T7181","span":{"begin":1644,"end":1648},"obj":"VBD"},{"id":"T7180","span":{"begin":1636,"end":1643},"obj":"NNS"},{"id":"T7179","span":{"begin":1635,"end":1754},"obj":"sentence"},{"id":"T7178","span":{"begin":1634,"end":1635},"obj":"."},{"id":"T7177","span":{"begin":1633,"end":1634},"obj":"-RRB-"},{"id":"T7176","span":{"begin":1620,"end":1623},"obj":"NNP"},{"id":"T7175","span":{"begin":1618,"end":1619},"obj":"NNP"},{"id":"T7174","span":{"begin":1624,"end":1633},"obj":"NNP"},{"id":"T7173","span":{"begin":1613,"end":1617},"obj":"NNP"},{"id":"T7172","span":{"begin":1609,"end":1612},"obj":"CC"},{"id":"T7171","span":{"begin":1596,"end":1597},"obj":"NNP"},{"id":"T7170","span":{"begin":1598,"end":1608},"obj":"NNP"},{"id":"T7169","span":{"begin":1593,"end":1595},"obj":"NNP"},{"id":"T7168","span":{"begin":1588,"end":1592},"obj":"IN"},{"id":"T7167","span":{"begin":1581,"end":1582},"obj":"DT"},{"id":"T7166","span":{"begin":1583,"end":1587},"obj":"NN"},{"id":"T7165","span":{"begin":1580,"end":1581},"obj":"-LRB-"},{"id":"T7164","span":{"begin":1576,"end":1579},"obj":"NN"},{"id":"T7163","span":{"begin":1564,"end":1575},"obj":"VBN"},{"id":"T7162","span":{"begin":1556,"end":1563},"obj":"JJ"},{"id":"T7161","span":{"begin":1551,"end":1555},"obj":"IN"},{"id":"T7160","span":{"begin":1547,"end":1550},"obj":"NN"},{"id":"T7159","span":{"begin":1544,"end":1546},"obj":"IN"},{"id":"T7158","span":{"begin":1529,"end":1533},"obj":"VBD"},{"id":"T7157","span":{"begin":1527,"end":1528},"obj":"-RRB-"},{"id":"T7156","span":{"begin":1525,"end":1527},"obj":"CD"},{"id":"T7155","span":{"begin":1524,"end":1525},"obj":"-LRB-"},{"id":"T7153","span":{"begin":1517,"end":1522},"obj":"NN"},{"id":"T7152","span":{"begin":1516,"end":1517},"obj":"-LRB-"},{"id":"T7151","span":{"begin":1507,"end":1515},"obj":"NN"},{"id":"T7150","span":{"begin":1503,"end":1506},"obj":"CC"},{"id":"T7149","span":{"begin":1501,"end":1502},"obj":"-RRB-"},{"id":"T7148","span":{"begin":1499,"end":1501},"obj":"CD"},{"id":"T7147","span":{"begin":1498,"end":1499},"obj":"-LRB-"},{"id":"T7146","span":{"begin":1488,"end":1497},"obj":"NN"},{"id":"T7145","span":{"begin":1478,"end":1487},"obj":"NNS"},{"id":"T7144","span":{"begin":1474,"end":1477},"obj":"DT"},{"id":"T7143","span":{"begin":1463,"end":1473},"obj":"VBG"},{"id":"T7142","span":{"begin":1534,"end":1543},"obj":"VBN"},{"id":"T7141","span":{"begin":1453,"end":1462},"obj":"NNS"},{"id":"T7140","span":{"begin":1448,"end":1452},"obj":"NN"},{"id":"T7139","span":{"begin":1447,"end":1635},"obj":"sentence"},{"id":"T7138","span":{"begin":1446,"end":1447},"obj":"."},{"id":"T7137","span":{"begin":1445,"end":1446},"obj":"-RRB-"},{"id":"T7136","span":{"begin":1435,"end":1445},"obj":"JJ"},{"id":"T7135","span":{"begin":1434,"end":1435},"obj":"-LRB-"},{"id":"T7134","span":{"begin":1410,"end":1420},"obj":"NN"},{"id":"T6917","span":{"begin":546,"end":547},"obj":"CD"},{"id":"T6916","span":{"begin":544,"end":545},"obj":"-RRB-"},{"id":"T6915","span":{"begin":537,"end":544},"obj":"JJ"},{"id":"T6914","span":{"begin":536,"end":537},"obj":"-LRB-"},{"id":"T6913","span":{"begin":589,"end":590},"obj":"NN"},{"id":"T6912","span":{"begin":530,"end":535},"obj":"NN"},{"id":"T6911","span":{"begin":525,"end":529},"obj":"VBD"},{"id":"T6910","span":{"begin":517,"end":524},"obj":"NNS"},{"id":"T6929","span":{"begin":585,"end":588},"obj":"NN"}],"relations":[{"id":"R4202","pred":"nmod","subj":"T6808","obj":"T6809"},{"id":"R4203","pred":"nummod","subj":"T6810","obj":"T6811"},{"id":"R4204","pred":"compound","subj":"T6811","obj":"T6809"},{"id":"R4205","pred":"punct","subj":"T6812","obj":"T6811"},{"id":"R4206","pred":"det","subj":"T6814","obj":"T6815"},{"id":"R4207","pred":"nsubj","subj":"T6815","obj":"T6820"},{"id":"R4208","pred":"nmod","subj":"T6816","obj":"T6815"},{"id":"R4209","pred":"cc","subj":"T6817","obj":"T6816"},{"id":"R4210","pred":"compound","subj":"T6818","obj":"T6819"},{"id":"R4211","pred":"conj","subj":"T6819","obj":"T6816"},{"id":"R4212","pred":"prep","subj":"T6821","obj":"T6820"},{"id":"R4213","pred":"prep","subj":"T6822","obj":"T6821"},{"id":"R4214","pred":"det","subj":"T6823","obj":"T6824"},{"id":"R4215","pred":"pobj","subj":"T6824","obj":"T6822"},{"id":"R4216","pred":"amod","subj":"T6825","obj":"T6826"},{"id":"R4217","pred":"nmod","subj":"T6826","obj":"T6824"},{"id":"R4218","pred":"nmod","subj":"T6827","obj":"T6826"},{"id":"R4219","pred":"cc","subj":"T6828","obj":"T6826"},{"id":"R4220","pred":"compound","subj":"T6829","obj":"T6830"},{"id":"R4221","pred":"conj","subj":"T6830","obj":"T6826"},{"id":"R4222","pred":"amod","subj":"T6831","obj":"T6824"},{"id":"R4223","pred":"prep","subj":"T6832","obj":"T6831"},{"id":"R4224","pred":"det","subj":"T6833","obj":"T6834"},{"id":"R4225","pred":"pobj","subj":"T6834","obj":"T6832"},{"id":"R4226","pred":"amod","subj":"T6835","obj":"T6836"},{"id":"R4227","pred":"nmod","subj":"T6836","obj":"T6837"},{"id":"R4228","pred":"compound","subj":"T6837","obj":"T6834"},{"id":"R4229","pred":"amod","subj":"T6838","obj":"T6839"},{"id":"R4230","pred":"compound","subj":"T6839","obj":"T6837"},{"id":"R4231","pred":"compound","subj":"T6840","obj":"T6834"},{"id":"R4232","pred":"punct","subj":"T6841","obj":"T6842"},{"id":"R4233","pred":"parataxis","subj":"T6842","obj":"T6821"},{"id":"R4234","pred":"punct","subj":"T6843","obj":"T6842"},{"id":"R4235","pred":"punct","subj":"T6844","obj":"T6820"},{"id":"R4236","pred":"amod","subj":"T6846","obj":"T6847"},{"id":"R4237","pred":"nsubjpass","subj":"T6847","obj":"T6851"},{"id":"R4238","pred":"nmod","subj":"T6848","obj":"T6847"},{"id":"R4239","pred":"cc","subj":"T6849","obj":"T6848"},{"id":"R4240","pred":"conj","subj":"T6850","obj":"T6848"},{"id":"R4241","pred":"auxpass","subj":"T6852","obj":"T6851"},{"id":"R4242","pred":"aux","subj":"T6853","obj":"T6854"},{"id":"R4243","pred":"advcl","subj":"T6854","obj":"T6851"},{"id":"R4244","pred":"nummod","subj":"T6855","obj":"T6856"},{"id":"R4245","pred":"dobj","subj":"T6856","obj":"T6854"},{"id":"R4246","pred":"compound","subj":"T6857","obj":"T6858"},{"id":"R4247","pred":"compound","subj":"T6858","obj":"T6856"},{"id":"R4248","pred":"prep","subj":"T6859","obj":"T6854"},{"id":"R4249","pred":"det","subj":"T6860","obj":"T6861"},{"id":"R4250","pred":"pobj","subj":"T6861","obj":"T6859"},{"id":"R4251","pred":"compound","subj":"T6862","obj":"T6861"},{"id":"R4252","pred":"compound","subj":"T6863","obj":"T6861"},{"id":"R4253","pred":"punct","subj":"T6864","obj":"T6865"},{"id":"R4254","pred":"parataxis","subj":"T6865","obj":"T6861"},{"id":"R4255","pred":"nmod","subj":"T6866","obj":"T6865"},{"id":"R4256","pred":"nummod","subj":"T6867","obj":"T6868"},{"id":"R4257","pred":"compound","subj":"T6868","obj":"T6865"},{"id":"R4258","pred":"punct","subj":"T6869","obj":"T6868"},{"id":"R4259","pred":"punct","subj":"T6870","obj":"T6865"},{"id":"R4260","pred":"npadvmod","subj":"T6871","obj":"T6865"},{"id":"R4261","pred":"punct","subj":"T6872","obj":"T6865"},{"id":"R4262","pred":"punct","subj":"T6873","obj":"T6861"},{"id":"R4263","pred":"dep","subj":"T6874","obj":"T6875"},{"id":"R4264","pred":"relcl","subj":"T6875","obj":"T6861"},{"id":"R4265","pred":"det","subj":"T6876","obj":"T6877"},{"id":"R4266","pred":"dobj","subj":"T6877","obj":"T6875"},{"id":"R4267","pred":"acl","subj":"T6878","obj":"T6877"},{"id":"R4268","pred":"prep","subj":"T6879","obj":"T6878"},{"id":"R4269","pred":"det","subj":"T6880","obj":"T6881"},{"id":"R4270","pred":"pobj","subj":"T6881","obj":"T6879"},{"id":"R4271","pred":"compound","subj":"T6882","obj":"T6881"},{"id":"R4272","pred":"compound","subj":"T6883","obj":"T6881"},{"id":"R4273","pred":"compound","subj":"T6884","obj":"T6885"},{"id":"R4274","pred":"compound","subj":"T6885","obj":"T6881"},{"id":"R4275","pred":"punct","subj":"T6886","obj":"T6851"},{"id":"R4276","pred":"nsubj","subj":"T6888","obj":"T6889"},{"id":"R4277","pred":"det","subj":"T6890","obj":"T6891"},{"id":"R4278","pred":"dobj","subj":"T6891","obj":"T6889"},{"id":"R4279","pred":"amod","subj":"T6892","obj":"T6891"},{"id":"R4280","pred":"compound","subj":"T6893","obj":"T6891"},{"id":"R4281","pred":"punct","subj":"T6894","obj":"T6895"},{"id":"R4282","pred":"parataxis","subj":"T6895","obj":"T6889"},{"id":"R4283","pred":"nmod","subj":"T6896","obj":"T6895"},{"id":"R4284","pred":"nmod","subj":"T6897","obj":"T6895"},{"id":"R4285","pred":"punct","subj":"T6898","obj":"T6899"},{"id":"R4286","pred":"prep","subj":"T6899","obj":"T6895"},{"id":"R4287","pred":"prep","subj":"T6900","obj":"T6895"},{"id":"R4288","pred":"det","subj":"T6901","obj":"T6902"},{"id":"R4289","pred":"pobj","subj":"T6902","obj":"T6900"},{"id":"R4290","pred":"amod","subj":"T6903","obj":"T6904"},{"id":"R4291","pred":"compound","subj":"T6904","obj":"T6902"},{"id":"R4292","pred":"punct","subj":"T6905","obj":"T6904"},{"id":"R4293","pred":"compound","subj":"T6906","obj":"T6902"},{"id":"R4294","pred":"punct","subj":"T6907","obj":"T6895"},{"id":"R4295","pred":"punct","subj":"T6908","obj":"T6889"},{"id":"R4296","pred":"nsubj","subj":"T6910","obj":"T6911"},{"id":"R4297","pred":"nmod","subj":"T6912","obj":"T6913"},{"id":"R4298","pred":"attr","subj":"T6913","obj":"T6911"},{"id":"R4299","pred":"punct","subj":"T6914","obj":"T6915"},{"id":"R4300","pred":"parataxis","subj":"T6915","obj":"T6913"},{"id":"R4301","pred":"punct","subj":"T6916","obj":"T6915"},{"id":"R4302","pred":"nummod","subj":"T6917","obj":"T6913"},{"id":"R4303","pred":"punct","subj":"T6918","obj":"T6917"},{"id":"R4304","pred":"punct","subj":"T6919","obj":"T6913"},{"id":"R4305","pred":"compound","subj":"T6920","obj":"T6913"},{"id":"R4306","pred":"compound","subj":"T6921","obj":"T6913"},{"id":"R4307","pred":"compound","subj":"T6922","obj":"T6913"},{"id":"R4308","pred":"compound","subj":"T6923","obj":"T6913"},{"id":"R4309","pred":"compound","subj":"T6924","obj":"T6913"},{"id":"R4310","pred":"compound","subj":"T6925","obj":"T6913"},{"id":"R4311","pred":"compound","subj":"T6926","obj":"T6913"},{"id":"R4312","pred":"compound","subj":"T6927","obj":"T6913"},{"id":"R4313","pred":"compound","subj":"T6928","obj":"T6913"},{"id":"R4314","pred":"compound","subj":"T6929","obj":"T6913"},{"id":"R4315","pred":"punct","subj":"T6930","obj":"T6913"},{"id":"R4316","pred":"nummod","subj":"T6931","obj":"T6913"},{"id":"R4317","pred":"punct","subj":"T6932","obj":"T6913"},{"id":"R4318","pred":"cc","subj":"T6933","obj":"T6913"},{"id":"R4319","pred":"nmod","subj":"T6934","obj":"T6935"},{"id":"R4320","pred":"conj","subj":"T6935","obj":"T6913"},{"id":"R4321","pred":"punct","subj":"T6936","obj":"T6937"},{"id":"R4322","pred":"parataxis","subj":"T6937","obj":"T6935"},{"id":"R4323","pred":"punct","subj":"T6938","obj":"T6937"},{"id":"R4324","pred":"nummod","subj":"T6939","obj":"T6935"},{"id":"R4325","pred":"punct","subj":"T6940","obj":"T6939"},{"id":"R4326","pred":"punct","subj":"T6941","obj":"T6935"},{"id":"R4327","pred":"compound","subj":"T6942","obj":"T6935"},{"id":"R4328","pred":"compound","subj":"T6943","obj":"T6935"},{"id":"R4329","pred":"compound","subj":"T6944","obj":"T6935"},{"id":"R4330","pred":"compound","subj":"T6945","obj":"T6935"},{"id":"R4331","pred":"compound","subj":"T6946","obj":"T6935"},{"id":"R4332","pred":"compound","subj":"T6947","obj":"T6935"},{"id":"R4333","pred":"compound","subj":"T6948","obj":"T6935"},{"id":"R4334","pred":"compound","subj":"T6949","obj":"T6935"},{"id":"R4335","pred":"compound","subj":"T6950","obj":"T6935"},{"id":"R4336","pred":"compound","subj":"T6951","obj":"T6935"},{"id":"R4337","pred":"compound","subj":"T6952","obj":"T6935"},{"id":"R4338","pred":"punct","subj":"T6953","obj":"T6935"},{"id":"R4339","pred":"nummod","subj":"T6954","obj":"T6935"},{"id":"R4340","pred":"punct","subj":"T6955","obj":"T6935"},{"id":"R4341","pred":"punct","subj":"T6956","obj":"T6911"},{"id":"R4342","pred":"nsubj","subj":"T6958","obj":"T6959"},{"id":"R4343","pred":"det","subj":"T6960","obj":"T6961"},{"id":"R4344","pred":"dobj","subj":"T6961","obj":"T6959"},{"id":"R4345","pred":"advmod","subj":"T6962","obj":"T6963"},{"id":"R4346","pred":"amod","subj":"T6963","obj":"T6961"},{"id":"R4347","pred":"npadvmod","subj":"T6964","obj":"T6965"},{"id":"R4348","pred":"amod","subj":"T6965","obj":"T6961"},{"id":"R4349","pred":"punct","subj":"T6966","obj":"T6965"},{"id":"R4350","pred":"amod","subj":"T6967","obj":"T6961"},{"id":"R4351","pred":"punct","subj":"T6968","obj":"T6969"},{"id":"R4352","pred":"parataxis","subj":"T6969","obj":"T6959"},{"id":"R4353","pred":"punct","subj":"T6970","obj":"T6971"},{"id":"R4354","pred":"prep","subj":"T6971","obj":"T6969"},{"id":"R4355","pred":"punct","subj":"T6972","obj":"T6969"},{"id":"R4356","pred":"punct","subj":"T6973","obj":"T6959"},{"id":"R4357","pred":"nsubj","subj":"T6975","obj":"T6976"},{"id":"R4358","pred":"nmod","subj":"T6977","obj":"T6978"},{"id":"R4359","pred":"attr","subj":"T6978","obj":"T6976"},{"id":"R4360","pred":"punct","subj":"T6979","obj":"T6980"},{"id":"R4361","pred":"parataxis","subj":"T6980","obj":"T6978"},{"id":"R4362","pred":"punct","subj":"T6981","obj":"T6980"},{"id":"R4363","pred":"nummod","subj":"T6982","obj":"T6978"},{"id":"R4364","pred":"punct","subj":"T6983","obj":"T6982"},{"id":"R4365","pred":"punct","subj":"T6984","obj":"T6978"},{"id":"R4366","pred":"compound","subj":"T6985","obj":"T6978"},{"id":"R4367","pred":"compound","subj":"T6986","obj":"T6978"},{"id":"R4368","pred":"compound","subj":"T6987","obj":"T6978"},{"id":"R4369","pred":"compound","subj":"T6988","obj":"T6978"},{"id":"R4370","pred":"compound","subj":"T6989","obj":"T6978"},{"id":"R4371","pred":"compound","subj":"T6990","obj":"T6978"},{"id":"R4372","pred":"compound","subj":"T6991","obj":"T6978"},{"id":"R4373","pred":"compound","subj":"T6992","obj":"T6978"},{"id":"R4374","pred":"compound","subj":"T6993","obj":"T6978"},{"id":"R4375","pred":"compound","subj":"T6994","obj":"T6978"},{"id":"R4376","pred":"punct","subj":"T6995","obj":"T6978"},{"id":"R4377","pred":"nummod","subj":"T6996","obj":"T6978"},{"id":"R4378","pred":"punct","subj":"T6997","obj":"T6978"},{"id":"R4379","pred":"cc","subj":"T6998","obj":"T6978"},{"id":"R4380","pred":"conj","subj":"T6999","obj":"T6978"},{"id":"R4381","pred":"punct","subj":"T7000","obj":"T7001"},{"id":"R4382","pred":"parataxis","subj":"T7001","obj":"T6999"},{"id":"R4383","pred":"punct","subj":"T7002","obj":"T7001"},{"id":"R4384","pred":"punct","subj":"T7003","obj":"T6976"},{"id":"R4385","pred":"compound","subj":"T7005","obj":"T7006"},{"id":"R4386","pred":"nsubj","subj":"T7006","obj":"T7008"},{"id":"R4387","pred":"punct","subj":"T7007","obj":"T7006"},{"id":"R4388","pred":"det","subj":"T7009","obj":"T7010"},{"id":"R4389","pred":"dobj","subj":"T7010","obj":"T7008"},{"id":"R4390","pred":"compound","subj":"T7011","obj":"T7010"},{"id":"R4391","pred":"cc","subj":"T7012","obj":"T7010"},{"id":"R4392","pred":"det","subj":"T7013","obj":"T7014"},{"id":"R4393","pred":"conj","subj":"T7014","obj":"T7010"},{"id":"R4394","pred":"npadvmod","subj":"T7015","obj":"T7016"},{"id":"R4395","pred":"amod","subj":"T7016","obj":"T7014"},{"id":"R4396","pred":"punct","subj":"T7017","obj":"T7016"},{"id":"R4397","pred":"punct","subj":"T7018","obj":"T7019"},{"id":"R4398","pred":"parataxis","subj":"T7019","obj":"T7008"},{"id":"R4399","pred":"punct","subj":"T7020","obj":"T7021"},{"id":"R4400","pred":"prep","subj":"T7021","obj":"T7019"},{"id":"R4401","pred":"punct","subj":"T7022","obj":"T7019"},{"id":"R4402","pred":"punct","subj":"T7023","obj":"T7008"},{"id":"R4403","pred":"nsubjpass","subj":"T7025","obj":"T7026"},{"id":"R4404","pred":"auxpass","subj":"T7027","obj":"T7026"},{"id":"R4405","pred":"prep","subj":"T7028","obj":"T7026"},{"id":"R4406","pred":"pcomp","subj":"T7029","obj":"T7028"},{"id":"R4407","pred":"dobj","subj":"T7030","obj":"T7029"},{"id":"R4408","pred":"prep","subj":"T7031","obj":"T7029"},{"id":"R4409","pred":"pobj","subj":"T7032","obj":"T7031"},{"id":"R4410","pred":"cc","subj":"T7033","obj":"T7032"},{"id":"R4411","pred":"conj","subj":"T7034","obj":"T7032"},{"id":"R4412","pred":"aux","subj":"T7035","obj":"T7036"},{"id":"R4413","pred":"advcl","subj":"T7036","obj":"T7029"},{"id":"R4414","pred":"prt","subj":"T7037","obj":"T7036"},{"id":"R4415","pred":"det","subj":"T7038","obj":"T7039"},{"id":"R4416","pred":"dobj","subj":"T7039","obj":"T7036"},{"id":"R4417","pred":"npadvmod","subj":"T7040","obj":"T7041"},{"id":"R4418","pred":"amod","subj":"T7041","obj":"T7039"},{"id":"R4419","pred":"punct","subj":"T7042","obj":"T7041"},{"id":"R4420","pred":"punct","subj":"T7043","obj":"T7029"},{"id":"R4421","pred":"cc","subj":"T7044","obj":"T7029"},{"id":"R4422","pred":"conj","subj":"T7045","obj":"T7029"},{"id":"R4423","pred":"det","subj":"T7046","obj":"T7047"},{"id":"R4424","pred":"dobj","subj":"T7047","obj":"T7045"},{"id":"R4425","pred":"amod","subj":"T7048","obj":"T7047"},{"id":"R4426","pred":"prep","subj":"T7049","obj":"T7047"},{"id":"R4427","pred":"det","subj":"T7050","obj":"T7051"},{"id":"R4428","pred":"pobj","subj":"T7051","obj":"T7049"},{"id":"R4429","pred":"nummod","subj":"T7052","obj":"T7051"},{"id":"R4430","pred":"compound","subj":"T7053","obj":"T7051"},{"id":"R4431","pred":"punct","subj":"T7054","obj":"T7026"},{"id":"R4432","pred":"det","subj":"T7056","obj":"T7057"},{"id":"R4433","pred":"nsubjpass","subj":"T7057","obj":"T7058"},{"id":"R4434","pred":"acl","subj":"T7059","obj":"T7057"},{"id":"R4435","pred":"prep","subj":"T7060","obj":"T7059"},{"id":"R4436","pred":"pobj","subj":"T7061","obj":"T7060"},{"id":"R4437","pred":"cc","subj":"T7062","obj":"T7060"},{"id":"R4438","pred":"conj","subj":"T7063","obj":"T7060"},{"id":"R4439","pred":"pobj","subj":"T7064","obj":"T7063"},{"id":"R4440","pred":"auxpass","subj":"T7065","obj":"T7058"},{"id":"R4441","pred":"aux","subj":"T7066","obj":"T7067"},{"id":"R4442","pred":"advcl","subj":"T7067","obj":"T7058"},{"id":"R4443","pred":"mark","subj":"T7068","obj":"T7069"},{"id":"R4444","pred":"ccomp","subj":"T7069","obj":"T7067"},{"id":"R4445","pred":"det","subj":"T7070","obj":"T7071"},{"id":"R4446","pred":"nsubjpass","subj":"T7071","obj":"T7069"},{"id":"R4447","pred":"aux","subj":"T7072","obj":"T7069"},{"id":"R4448","pred":"auxpass","subj":"T7073","obj":"T7069"},{"id":"R4449","pred":"punct","subj":"T7074","obj":"T7058"},{"id":"R4450","pred":"det","subj":"T7076","obj":"T7077"},{"id":"R4451","pred":"nsubjpass","subj":"T7077","obj":"T7081"},{"id":"R4452","pred":"compound","subj":"T7078","obj":"T7079"},{"id":"R4453","pred":"compound","subj":"T7079","obj":"T7077"},{"id":"R4454","pred":"compound","subj":"T7080","obj":"T7079"},{"id":"R4455","pred":"acl","subj":"T7082","obj":"T7077"},{"id":"R4456","pred":"det","subj":"T7083","obj":"T7084"},{"id":"R4457","pred":"dobj","subj":"T7084","obj":"T7082"},{"id":"R4458","pred":"appos","subj":"T7085","obj":"T7084"},{"id":"R4459","pred":"punct","subj":"T7086","obj":"T7087"},{"id":"R4460","pred":"prep","subj":"T7087","obj":"T7085"},{"id":"R4461","pred":"punct","subj":"T7088","obj":"T7084"},{"id":"R4462","pred":"appos","subj":"T7089","obj":"T7084"},{"id":"R4463","pred":"punct","subj":"T7090","obj":"T7084"},{"id":"R4464","pred":"auxpass","subj":"T7091","obj":"T7081"},{"id":"R4465","pred":"prep","subj":"T7092","obj":"T7081"},{"id":"R4466","pred":"det","subj":"T7093","obj":"T7094"},{"id":"R4467","pred":"pobj","subj":"T7094","obj":"T7092"},{"id":"R4468","pred":"amod","subj":"T7095","obj":"T7094"},{"id":"R4469","pred":"prep","subj":"T7096","obj":"T7094"},{"id":"R4470","pred":"det","subj":"T7097","obj":"T7098"},{"id":"R4471","pred":"pobj","subj":"T7098","obj":"T7096"},{"id":"R4472","pred":"compound","subj":"T7099","obj":"T7098"},{"id":"R4473","pred":"punct","subj":"T7100","obj":"T7092"},{"id":"R4474","pred":"cc","subj":"T7101","obj":"T7092"},{"id":"R4475","pred":"conj","subj":"T7102","obj":"T7092"},{"id":"R4476","pred":"det","subj":"T7103","obj":"T7104"},{"id":"R4477","pred":"dobj","subj":"T7104","obj":"T7102"},{"id":"R4478","pred":"amod","subj":"T7105","obj":"T7104"},{"id":"R4479","pred":"compound","subj":"T7106","obj":"T7104"},{"id":"R4480","pred":"nmod","subj":"T7107","obj":"T7108"},{"id":"R4481","pred":"appos","subj":"T7108","obj":"T7104"},{"id":"R4482","pred":"nummod","subj":"T7109","obj":"T7108"},{"id":"R4483","pred":"punct","subj":"T7110","obj":"T7109"},{"id":"R4484","pred":"punct","subj":"T7111","obj":"T7108"},{"id":"R4485","pred":"compound","subj":"T7112","obj":"T7108"},{"id":"R4486","pred":"compound","subj":"T7113","obj":"T7108"},{"id":"R4487","pred":"compound","subj":"T7114","obj":"T7108"},{"id":"R4488","pred":"compound","subj":"T7115","obj":"T7108"},{"id":"R4489","pred":"compound","subj":"T7116","obj":"T7108"},{"id":"R4490","pred":"compound","subj":"T7117","obj":"T7108"},{"id":"R4491","pred":"compound","subj":"T7118","obj":"T7108"},{"id":"R4492","pred":"compound","subj":"T7119","obj":"T7108"},{"id":"R4493","pred":"compound","subj":"T7120","obj":"T7108"},{"id":"R4494","pred":"compound","subj":"T7121","obj":"T7108"},{"id":"R4495","pred":"compound","subj":"T7122","obj":"T7108"},{"id":"R4496","pred":"compound","subj":"T7123","obj":"T7108"},{"id":"R4497","pred":"compound","subj":"T7124","obj":"T7108"},{"id":"R4498","pred":"punct","subj":"T7125","obj":"T7108"},{"id":"R4499","pred":"nummod","subj":"T7126","obj":"T7108"},{"id":"R4500","pred":"punct","subj":"T7127","obj":"T7108"},{"id":"R4501","pred":"punct","subj":"T7128","obj":"T7104"},{"id":"R4502","pred":"dep","subj":"T7129","obj":"T7130"},{"id":"R4503","pred":"relcl","subj":"T7130","obj":"T7104"},{"id":"R4504","pred":"det","subj":"T7131","obj":"T7132"},{"id":"R4505","pred":"dobj","subj":"T7132","obj":"T7130"},{"id":"R4506","pred":"compound","subj":"T7133","obj":"T7132"},{"id":"R4507","pred":"compound","subj":"T7134","obj":"T7132"},{"id":"R4508","pred":"punct","subj":"T7135","obj":"T7136"},{"id":"R4509","pred":"parataxis","subj":"T7136","obj":"T7104"},{"id":"R4510","pred":"punct","subj":"T7137","obj":"T7136"},{"id":"R4511","pred":"punct","subj":"T7138","obj":"T7081"},{"id":"R4512","pred":"compound","subj":"T7140","obj":"T7141"},{"id":"R4513","pred":"nsubjpass","subj":"T7141","obj":"T7142"},{"id":"R4514","pred":"acl","subj":"T7143","obj":"T7141"},{"id":"R4515","pred":"det","subj":"T7144","obj":"T7145"},{"id":"R4516","pred":"dobj","subj":"T7145","obj":"T7143"},{"id":"R4517","pred":"appos","subj":"T7146","obj":"T7145"},{"id":"R4518","pred":"punct","subj":"T7147","obj":"T7148"},{"id":"R4519","pred":"parataxis","subj":"T7148","obj":"T7146"},{"id":"R4520","pred":"punct","subj":"T7149","obj":"T7148"},{"id":"R4521","pred":"cc","subj":"T7150","obj":"T7146"},{"id":"R4522","pred":"conj","subj":"T7151","obj":"T7146"},{"id":"R4523","pred":"punct","subj":"T7152","obj":"T7151"},{"id":"R4524","pred":"appos","subj":"T7153","obj":"T7151"},{"id":"R4525","pred":"punct","subj":"T7154","obj":"T7151"},{"id":"R4526","pred":"punct","subj":"T7155","obj":"T7156"},{"id":"R4527","pred":"parataxis","subj":"T7156","obj":"T7151"},{"id":"R4528","pred":"punct","subj":"T7157","obj":"T7156"},{"id":"R4529","pred":"auxpass","subj":"T7158","obj":"T7142"},{"id":"R4530","pred":"prep","subj":"T7159","obj":"T7142"},{"id":"R4531","pred":"pobj","subj":"T7160","obj":"T7159"},{"id":"R4532","pred":"prep","subj":"T7161","obj":"T7160"},{"id":"R4533","pred":"amod","subj":"T7162","obj":"T7163"},{"id":"R4534","pred":"amod","subj":"T7163","obj":"T7164"},{"id":"R4535","pred":"pobj","subj":"T7164","obj":"T7161"},{"id":"R4536","pred":"punct","subj":"T7165","obj":"T7166"},{"id":"R4537","pred":"parataxis","subj":"T7166","obj":"T7164"},{"id":"R4538","pred":"det","subj":"T7167","obj":"T7166"},{"id":"R4539","pred":"prep","subj":"T7168","obj":"T7166"},{"id":"R4540","pred":"compound","subj":"T7169","obj":"T7170"},{"id":"R4541","pred":"pobj","subj":"T7170","obj":"T7168"},{"id":"R4542","pred":"compound","subj":"T7171","obj":"T7170"},{"id":"R4543","pred":"cc","subj":"T7172","obj":"T7170"},{"id":"R4544","pred":"compound","subj":"T7173","obj":"T7174"},{"id":"R4545","pred":"conj","subj":"T7174","obj":"T7170"},{"id":"R4546","pred":"compound","subj":"T7175","obj":"T7174"},{"id":"R4547","pred":"compound","subj":"T7176","obj":"T7174"},{"id":"R4548","pred":"punct","subj":"T7177","obj":"T7166"},{"id":"R4549","pred":"punct","subj":"T7178","obj":"T7142"},{"id":"R4550","pred":"nsubj","subj":"T7180","obj":"T7181"},{"id":"R4551","pred":"nmod","subj":"T7182","obj":"T7183"},{"id":"R4552","pred":"attr","subj":"T7183","obj":"T7181"},{"id":"R4553","pred":"punct","subj":"T7184","obj":"T7185"},{"id":"R4554","pred":"parataxis","subj":"T7185","obj":"T7183"},{"id":"R4555","pred":"punct","subj":"T7186","obj":"T7185"},{"id":"R4556","pred":"nummod","subj":"T7187","obj":"T7183"},{"id":"R4557","pred":"punct","subj":"T7188","obj":"T7187"},{"id":"R4558","pred":"punct","subj":"T7189","obj":"T7183"},{"id":"R4559","pred":"compound","subj":"T7190","obj":"T7183"},{"id":"R4560","pred":"compound","subj":"T7191","obj":"T7183"},{"id":"R4561","pred":"compound","subj":"T7192","obj":"T7183"},{"id":"R4562","pred":"compound","subj":"T7193","obj":"T7183"},{"id":"R4563","pred":"compound","subj":"T7194","obj":"T7183"},{"id":"R4564","pred":"compound","subj":"T7195","obj":"T7183"},{"id":"R4565","pred":"punct","subj":"T7196","obj":"T7183"},{"id":"R4566","pred":"nummod","subj":"T7197","obj":"T7183"},{"id":"R4567","pred":"punct","subj":"T7198","obj":"T7183"},{"id":"R4568","pred":"cc","subj":"T7199","obj":"T7183"},{"id":"R4569","pred":"nmod","subj":"T7200","obj":"T7201"},{"id":"R4570","pred":"conj","subj":"T7201","obj":"T7183"},{"id":"R4571","pred":"punct","subj":"T7202","obj":"T7203"},{"id":"R4572","pred":"parataxis","subj":"T7203","obj":"T7201"},{"id":"R4573","pred":"punct","subj":"T7204","obj":"T7203"},{"id":"R4574","pred":"nummod","subj":"T7205","obj":"T7201"},{"id":"R4575","pred":"punct","subj":"T7206","obj":"T7205"},{"id":"R4576","pred":"punct","subj":"T7207","obj":"T7201"},{"id":"R4577","pred":"compound","subj":"T7208","obj":"T7201"},{"id":"R4578","pred":"compound","subj":"T7209","obj":"T7201"},{"id":"R4579","pred":"compound","subj":"T7210","obj":"T7201"},{"id":"R4580","pred":"compound","subj":"T7211","obj":"T7201"},{"id":"R4581","pred":"compound","subj":"T7212","obj":"T7201"},{"id":"R4582","pred":"compound","subj":"T7213","obj":"T7201"},{"id":"R4583","pred":"compound","subj":"T7214","obj":"T7201"},{"id":"R4584","pred":"compound","subj":"T7215","obj":"T7201"},{"id":"R4585","pred":"compound","subj":"T7216","obj":"T7201"},{"id":"R4586","pred":"compound","subj":"T7217","obj":"T7201"},{"id":"R4587","pred":"punct","subj":"T7218","obj":"T7201"},{"id":"R4588","pred":"nummod","subj":"T7219","obj":"T7201"},{"id":"R4589","pred":"punct","subj":"T7220","obj":"T7201"},{"id":"R4590","pred":"punct","subj":"T7221","obj":"T7181"},{"id":"R4591","pred":"det","subj":"T7223","obj":"T7224"},{"id":"R4592","pred":"nsubjpass","subj":"T7224","obj":"T7226"},{"id":"R4593","pred":"compound","subj":"T7225","obj":"T7224"},{"id":"R4594","pred":"auxpass","subj":"T7227","obj":"T7226"},{"id":"R4595","pred":"prep","subj":"T7228","obj":"T7226"},{"id":"R4596","pred":"pobj","subj":"T7229","obj":"T7228"},{"id":"R4597","pred":"cc","subj":"T7230","obj":"T7229"},{"id":"R4598","pred":"conj","subj":"T7231","obj":"T7229"},{"id":"R4599","pred":"aux","subj":"T7232","obj":"T7233"},{"id":"R4600","pred":"advcl","subj":"T7233","obj":"T7226"},{"id":"R4601","pred":"det","subj":"T7234","obj":"T7235"},{"id":"R4602","pred":"dobj","subj":"T7235","obj":"T7233"},{"id":"R4603","pred":"amod","subj":"T7236","obj":"T7235"},{"id":"R4604","pred":"npadvmod","subj":"T7237","obj":"T7238"},{"id":"R4605","pred":"amod","subj":"T7238","obj":"T7235"},{"id":"R4606","pred":"punct","subj":"T7239","obj":"T7238"},{"id":"R4607","pred":"punct","subj":"T7240","obj":"T7226"},{"id":"R4608","pred":"cc","subj":"T7241","obj":"T7226"},{"id":"R4609","pred":"det","subj":"T7242","obj":"T7243"},{"id":"R4610","pred":"nsubjpass","subj":"T7243","obj":"T7245"},{"id":"R4611","pred":"compound","subj":"T7244","obj":"T7243"},{"id":"R4612","pred":"conj","subj":"T7245","obj":"T7226"},{"id":"R4613","pred":"auxpass","subj":"T7246","obj":"T7245"},{"id":"R4614","pred":"punct","subj":"T7247","obj":"T7245"}],"text":"Yeast two-hybrid constructs\nAll cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27]. Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain. PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein). Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'. PAX6CTP contains the newly defined C-terminal peptide alone (391–422). Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R). PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390). This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites. All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.\nA PAX6 PST domain construct containing the mutation 1627A\u003eG (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).\ncDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen). Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'. The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T6807","span":{"begin":1874,"end":1882},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T6806","span":{"begin":1861,"end":1869},"obj":"SO_EXT:biological_sequence"},{"id":"T6805","span":{"begin":1854,"end":1860},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T6804","span":{"begin":1837,"end":1844},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6803","span":{"begin":1826,"end":1836},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6802","span":{"begin":1807,"end":1814},"obj":"GO_EXT:biological_release"},{"id":"T6801","span":{"begin":1781,"end":1784},"obj":"MOP:0000780"},{"id":"T6800","span":{"begin":1767,"end":1776},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T6799","span":{"begin":1702,"end":1703},"obj":"SO:0001031"},{"id":"T6798","span":{"begin":1656,"end":1657},"obj":"SO:0001030"},{"id":"T6797","span":{"begin":1636,"end":1643},"obj":"SO_EXT:0000112"},{"id":"T6796","span":{"begin":1576,"end":1579},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T6795","span":{"begin":1556,"end":1575},"obj":"GO:0001171"},{"id":"T6794","span":{"begin":1478,"end":1487},"obj":"SO_EXT:sequence_alteration_entity_or_process"},{"id":"T6793","span":{"begin":1453,"end":1462},"obj":"SO_EXT:biological_sequence"},{"id":"T6792","span":{"begin":1448,"end":1452},"obj":"SO_EXT:cDNA"},{"id":"T6791","span":{"begin":1421,"end":1433},"obj":"SO_EXT:sequence_substitution_entity_or_process"},{"id":"T6790","span":{"begin":1410,"end":1420},"obj":"CHEBI_SO_EXT:nucleotide"},{"id":"T6789","span":{"begin":1403,"end":1409},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T6788","span":{"begin":1309,"end":1315},"obj":"SO_EXT:0000132"},{"id":"T6787","span":{"begin":1297,"end":1304},"obj":"_FRAGMENT"},{"id":"T6786","span":{"begin":1272,"end":1281},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T6785","span":{"begin":1217,"end":1218},"obj":"CHEBI_EXT:guanine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group"},{"id":"T6784","span":{"begin":1202,"end":1210},"obj":"SO_EXT:sequence_alteration_entity_or_process"},{"id":"T6783","span":{"begin":1177,"end":1186},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T6782","span":{"begin":1170,"end":1176},"obj":"SO_EXT:0000417"},{"id":"T6781","span":{"begin":1161,"end":1165},"obj":"PR_EXT:000012318"},{"id":"T6780","span":{"begin":1033,"end":1050},"obj":"SO_EXT:0000061"},{"id":"T6779","span":{"begin":988,"end":997},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T6778","span":{"begin":975,"end":982},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6777","span":{"begin":964,"end":974},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6776","span":{"begin":913,"end":920},"obj":"MOP:0000780"},{"id":"T6775","span":{"begin":877,"end":884},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6774","span":{"begin":866,"end":876},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6773","span":{"begin":849,"end":855},"obj":"SO_EXT:0000417"},{"id":"T6772","span":{"begin":817,"end":818},"obj":"SO:0001031"},{"id":"T6771","span":{"begin":754,"end":755},"obj":"SO:0001030"},{"id":"T6770","span":{"begin":734,"end":741},"obj":"SO_EXT:0000112"},{"id":"T6769","span":{"begin":709,"end":716},"obj":"CHEBI_SO_EXT:peptide_or_peptide_region"},{"id":"T6768","span":{"begin":698,"end":708},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T6767","span":{"begin":605,"end":606},"obj":"SO:0001031"},{"id":"T6766","span":{"begin":537,"end":544},"obj":"SO:0001030"},{"id":"T6765","span":{"begin":517,"end":524},"obj":"SO_EXT:0000112"},{"id":"T6764","span":{"begin":507,"end":514},"obj":"CHEBI_PR_EXT:protein"},{"id":"T6763","span":{"begin":502,"end":506},"obj":"PR_EXT:000012318"},{"id":"T6762","span":{"begin":463,"end":474},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T6761","span":{"begin":455,"end":461},"obj":"SO_EXT:0000417"},{"id":"T6760","span":{"begin":416,"end":422},"obj":"SO_EXT:0000417"},{"id":"T6759","span":{"begin":408,"end":415},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T6758","span":{"begin":404,"end":407},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T6757","span":{"begin":399,"end":403},"obj":"PR_EXT:P04386"},{"id":"T6756","span":{"begin":393,"end":398},"obj":"NCBITaxon_EXT:yeast"},{"id":"T6755","span":{"begin":380,"end":385},"obj":"SO_EXT:sequence_fusion_process"},{"id":"T6754","span":{"begin":372,"end":379},"obj":"CHEBI_PR_EXT:protein"},{"id":"T6753","span":{"begin":305,"end":311},"obj":"SO_EXT:0000440"},{"id":"T6752","span":{"begin":294,"end":304},"obj":"GO:0010467"},{"id":"T6751","span":{"begin":269,"end":279},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T6750","span":{"begin":264,"end":268},"obj":"SO_EXT:cDNA"},{"id":"T6749","span":{"begin":259,"end":263},"obj":"PR_EXT:000012318"},{"id":"T6748","span":{"begin":164,"end":171},"obj":"SO_EXT:sequence_variant_or_variation"},{"id":"T6747","span":{"begin":156,"end":163},"obj":"SO_EXT:0001023"},{"id":"T6746","span":{"begin":151,"end":155},"obj":"PR_EXT:000012318"},{"id":"T6745","span":{"begin":145,"end":150},"obj":"NCBITaxon:9606"},{"id":"T6744","span":{"begin":116,"end":125},"obj":"SO_EXT:biological_sequence"},{"id":"T6743","span":{"begin":98,"end":105},"obj":"CHEBI_PR_EXT:protein"},{"id":"T6742","span":{"begin":89,"end":93},"obj":"SO_EXT:cDNA"},{"id":"T6741","span":{"begin":84,"end":88},"obj":"PR_EXT:000012318"},{"id":"T6740","span":{"begin":78,"end":83},"obj":"NCBITaxon:9606"},{"id":"T6739","span":{"begin":41,"end":51},"obj":"CHEBI_SO_EXT:amino_acid"},{"id":"T6738","span":{"begin":32,"end":36},"obj":"SO_EXT:cDNA"},{"id":"T6737","span":{"begin":17,"end":27},"obj":"SO_EXT:engineered_biological_sequence"},{"id":"T6736","span":{"begin":0,"end":5},"obj":"NCBITaxon_EXT:yeast"}],"relations":[{"id":"R4201","pred":"_lexicallyChainedTo","subj":"T6788","obj":"T6787"}],"text":"Yeast two-hybrid constructs\nAll cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27]. Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain. PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein). Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'. PAX6CTP contains the newly defined C-terminal peptide alone (391–422). Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R). PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390). This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites. All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.\nA PAX6 PST domain construct containing the mutation 1627A\u003eG (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).\ncDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen). Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'. The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T6719","span":{"begin":537,"end":544},"obj":"SO:0001030"},{"id":"T6718","span":{"begin":517,"end":524},"obj":"SO:0000112"},{"id":"T6717","span":{"begin":502,"end":506},"obj":"PR:000012318"},{"id":"T6716","span":{"begin":455,"end":461},"obj":"SO:0000417"},{"id":"T6715","span":{"begin":416,"end":422},"obj":"SO:0000417"},{"id":"T6714","span":{"begin":399,"end":403},"obj":"PR:P04386"},{"id":"T6713","span":{"begin":305,"end":311},"obj":"SO:0000440"},{"id":"T6712","span":{"begin":294,"end":304},"obj":"GO:0010467"},{"id":"T6711","span":{"begin":259,"end":263},"obj":"PR:000012318"},{"id":"T6710","span":{"begin":156,"end":163},"obj":"SO:0001023"},{"id":"T6709","span":{"begin":151,"end":155},"obj":"PR:000012318"},{"id":"T6708","span":{"begin":145,"end":150},"obj":"NCBITaxon:9606"},{"id":"T6707","span":{"begin":84,"end":88},"obj":"PR:000012318"},{"id":"T6706","span":{"begin":78,"end":83},"obj":"NCBITaxon:9606"},{"id":"T6735","span":{"begin":1781,"end":1784},"obj":"MOP:0000780"},{"id":"T6734","span":{"begin":1702,"end":1703},"obj":"SO:0001031"},{"id":"T6733","span":{"begin":1656,"end":1657},"obj":"SO:0001030"},{"id":"T6732","span":{"begin":1636,"end":1643},"obj":"SO:0000112"},{"id":"T6731","span":{"begin":1556,"end":1575},"obj":"GO:0001171"},{"id":"T6730","span":{"begin":1309,"end":1315},"obj":"SO:0000132"},{"id":"T6729","span":{"begin":1297,"end":1304},"obj":"_FRAGMENT"},{"id":"T6728","span":{"begin":1170,"end":1176},"obj":"SO:0000417"},{"id":"T6727","span":{"begin":1161,"end":1165},"obj":"PR:000012318"},{"id":"T6726","span":{"begin":1033,"end":1050},"obj":"SO:0000061"},{"id":"T6725","span":{"begin":913,"end":920},"obj":"MOP:0000780"},{"id":"T6724","span":{"begin":849,"end":855},"obj":"SO:0000417"},{"id":"T6723","span":{"begin":817,"end":818},"obj":"SO:0001031"},{"id":"T6722","span":{"begin":754,"end":755},"obj":"SO:0001030"},{"id":"T6721","span":{"begin":734,"end":741},"obj":"SO:0000112"},{"id":"T6720","span":{"begin":605,"end":606},"obj":"SO:0001031"}],"relations":[{"id":"R4200","pred":"_lexicallyChainedTo","subj":"T6730","obj":"T6729"}],"text":"Yeast two-hybrid constructs\nAll cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27]. Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain. PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein). Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'. PAX6CTP contains the newly defined C-terminal peptide alone (391–422). Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R). PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390). This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites. All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.\nA PAX6 PST domain construct containing the mutation 1627A\u003eG (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).\ncDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen). Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'. The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted."}