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retinal RNA samples were isolated from P0, P6, P14, and P21 Nr2e3 mutant mice using the Trizol reagent (Gibco, San Diego, California. United States). Total retinal RNA samples from age-matched wild-type C57BL/6 mice were used as controls. Individual total RNA samples were derived from four retinas (pooled from two animals). All microarray experiments were performed in triplicate, in each case with separate RNA preparations. Microarray experiments with cDNAs were performed with the P0, P6, and P14 derived samples. Probes were labeled with either Cy3 or Cy5 using the Array 900 kit from Genisphere (Hatfield, Pennsylvania, United States) starting with 5 μg of total RNA according to the manufacturer's instructions. Wild-type control probes were compared to mutant on the same microarray. In two of the three replicates, the mutant probe was labeled with Cy3 and the wild type with Cy5, and in the third replicate the dyes were swapped. Labeled probe was hybridized to microarray slides spotted with approximately 11,500 cDNA clones from the brain molecular anatomy project library (kind gift of B. Soares, University of Iowa; see http://trans.nih.gov/bmap/index.htm for details) and 500 cDNA clones from our lab collection. Slides were printed and hybridized as described [59,60]. After hybridization and washing of slides according to the manufacturer's instructions (Genisphere), the slides were scanned on an Axon Instruments (Union City, California, United States) GenePix 4000 scanner and images were analyzed using the accompanying GenePix Pro software package. The complete raw cDNA microarray data set are available in Tables S6–S14."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T12678","span":{"begin":6,"end":13},"obj":"UBERON:0000966"},{"id":"T12679","span":{"begin":14,"end":17},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T12680","span":{"begin":66,"end":71},"obj":"PR_EXT:000011403"},{"id":"T12681","span":{"begin":72,"end":78},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T12682","span":{"begin":79,"end":83},"obj":"NCBITaxon:10088"},{"id":"T12683","span":{"begin":101,"end":108},"obj":"CHEBI_EXT:33893"},{"id":"T12684","span":{"begin":162,"end":169},"obj":"UBERON:0000966"},{"id":"T12685","span":{"begin":170,"end":173},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T12686","span":{"begin":199,"end":208},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T12687","span":{"begin":217,"end":221},"obj":"NCBITaxon:10088"},{"id":"T12688","span":{"begin":262,"end":265},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T12689","span":{"begin":297,"end":304},"obj":"UBERON:0000966"},{"id":"T12690","span":{"begin":322,"end":329},"obj":"NCBITaxon:33208"},{"id":"T12691","span":{"begin":416,"end":419},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T12692","span":{"begin":462,"end":467},"obj":"SO_EXT:cDNA"},{"id":"T12693","span":{"begin":525,"end":531},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T12694","span":{"begin":537,"end":544},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T12695","span":{"begin":557,"end":560},"obj":"CHEBI:37987"},{"id":"T12696","span":{"begin":564,"end":567},"obj":"CHEBI:37989"},{"id":"T12697","span":{"begin":676,"end":679},"obj":"CHEBI_SO_EXT:RNA"},{"id":"T12698","span":{"begin":726,"end":735},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T12699","span":{"begin":744,"end":750},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T12700","span":{"begin":768,"end":774},"obj":"SO_EXT:sequence_altered_entity"},{"id":"T12701","span":{"begin":835,"end":841},"obj":"SO_EXT:sequence_altered_entity_or_alteration_process"},{"id":"T12702","span":{"begin":842,"end":847},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T12703","span":{"begin":852,"end":859},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T12704","span":{"begin":865,"end":868},"obj":"CHEBI:37987"},{"id":"T12705","span":{"begin":877,"end":886},"obj":"SO_EXT:wild_type_entity_or_quality"},{"id":"T12706","span":{"begin":892,"end":895},"obj":"CHEBI:37989"},{"id":"T12707","span":{"begin":928,"end":932},"obj":"CHEBI_EXT:37958"},{"id":"T12708","span":{"begin":947,"end":954},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T12709","span":{"begin":955,"end":960},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T12710","span":{"begin":965,"end":975},"obj":"GO:0097617"},{"id":"T12711","span":{"begin":1031,"end":1042},"obj":"SO_EXT:0000317"},{"id":"T12712","span":{"begin":1058,"end":1067},"obj":"CHEBI_EXT:polyatomic_entity_or_group"},{"id":"T12713","span":{"begin":1068,"end":1075},"obj":"UBERON:0001062"},{"id":"T12714","span":{"begin":1198,"end":1209},"obj":"SO_EXT:0000317"},{"id":"T12715","span":{"begin":1259,"end":1269},"obj":"GO:0097617"},{"id":"T12716","span":{"begin":1298,"end":1311},"obj":"GO:0097617"},{"id":"T12717","span":{"begin":1596,"end":1600},"obj":"SO_EXT:cDNA"}],"text":"Total retinal RNA samples were isolated from P0, P6, P14, and P21 Nr2e3 mutant mice using the Trizol reagent (Gibco, San Diego, California. United States). Total retinal RNA samples from age-matched wild-type C57BL/6 mice were used as controls. Individual total RNA samples were derived from four retinas (pooled from two animals). All microarray experiments were performed in triplicate, in each case with separate RNA preparations. Microarray experiments with cDNAs were performed with the P0, P6, and P14 derived samples. Probes were labeled with either Cy3 or Cy5 using the Array 900 kit from Genisphere (Hatfield, Pennsylvania, United States) starting with 5 μg of total RNA according to the manufacturer's instructions. Wild-type control probes were compared to mutant on the same microarray. In two of the three replicates, the mutant probe was labeled with Cy3 and the wild type with Cy5, and in the third replicate the dyes were swapped. Labeled probe was hybridized to microarray slides spotted with approximately 11,500 cDNA clones from the brain molecular anatomy project library (kind gift of B. Soares, University of Iowa; see http://trans.nih.gov/bmap/index.htm for details) and 500 cDNA clones from our lab collection. Slides were printed and hybridized as described [59,60]. After hybridization and washing of slides according to the manufacturer's instructions (Genisphere), the slides were scanned on an Axon Instruments (Union City, California, United States) GenePix 4000 scanner and images were analyzed using the accompanying GenePix Pro software package. The complete raw cDNA microarray data set are available in Tables S6–S14."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T12641","span":{"begin":6,"end":13},"obj":"UBERON:0000966"},{"id":"T12642","span":{"begin":66,"end":71},"obj":"PR:000011403"},{"id":"T12643","span":{"begin":79,"end":83},"obj":"NCBITaxon:10088"},{"id":"T12644","span":{"begin":101,"end":108},"obj":"CHEBI:33893"},{"id":"T12645","span":{"begin":162,"end":169},"obj":"UBERON:0000966"},{"id":"T12646","span":{"begin":217,"end":221},"obj":"NCBITaxon:10088"},{"id":"T12647","span":{"begin":297,"end":304},"obj":"UBERON:0000966"},{"id":"T12648","span":{"begin":322,"end":329},"obj":"NCBITaxon:33208"},{"id":"T12649","span":{"begin":557,"end":560},"obj":"CHEBI:37987"},{"id":"T12650","span":{"begin":564,"end":567},"obj":"CHEBI:37989"},{"id":"T12651","span":{"begin":865,"end":868},"obj":"CHEBI:37987"},{"id":"T12652","span":{"begin":892,"end":895},"obj":"CHEBI:37989"},{"id":"T12653","span":{"begin":928,"end":932},"obj":"CHEBI:37958"},{"id":"T12654","span":{"begin":965,"end":975},"obj":"GO:0097617"},{"id":"T12655","span":{"begin":1031,"end":1042},"obj":"SO:0000317"},{"id":"T12656","span":{"begin":1068,"end":1075},"obj":"UBERON:0001062"},{"id":"T12657","span":{"begin":1198,"end":1209},"obj":"SO:0000317"},{"id":"T12658","span":{"begin":1259,"end":1269},"obj":"GO:0097617"},{"id":"T12659","span":{"begin":1298,"end":1311},"obj":"GO:0097617"}],"text":"Total retinal RNA samples were isolated from P0, P6, P14, and P21 Nr2e3 mutant mice using the Trizol reagent (Gibco, San Diego, California. United States). Total retinal RNA samples from age-matched wild-type C57BL/6 mice were used as controls. Individual total RNA samples were derived from four retinas (pooled from two animals). All microarray experiments were performed in triplicate, in each case with separate RNA preparations. Microarray experiments with cDNAs were performed with the P0, P6, and P14 derived samples. Probes were labeled with either Cy3 or Cy5 using the Array 900 kit from Genisphere (Hatfield, Pennsylvania, United States) starting with 5 μg of total RNA according to the manufacturer's instructions. Wild-type control probes were compared to mutant on the same microarray. In two of the three replicates, the mutant probe was labeled with Cy3 and the wild type with Cy5, and in the third replicate the dyes were swapped. Labeled probe was hybridized to microarray slides spotted with approximately 11,500 cDNA clones from the brain molecular anatomy project library (kind gift of B. Soares, University of Iowa; see http://trans.nih.gov/bmap/index.htm for details) and 500 cDNA clones from our lab collection. Slides were printed and hybridized as described [59,60]. After hybridization and washing of slides according to the manufacturer's instructions (Genisphere), the slides were scanned on an Axon Instruments (Union City, California, United States) GenePix 4000 scanner and images were analyzed using the accompanying GenePix Pro software package. The complete raw cDNA microarray data set are available in Tables S6–S14."}

{"project":"2_test","denotations":[{"id":"16110338-12692551-85798572","span":{"begin":1284,"end":1286},"obj":"12692551"},{"id":"16110338-14734810-85798573","span":{"begin":1287,"end":1289},"obj":"14734810"},{"id":"T69510","span":{"begin":1284,"end":1286},"obj":"12692551"},{"id":"T42515","span":{"begin":1287,"end":1289},"obj":"14734810"}],"text":"Total retinal RNA samples were isolated from P0, P6, P14, and P21 Nr2e3 mutant mice using the Trizol reagent (Gibco, San Diego, California. United States). Total retinal RNA samples from age-matched wild-type C57BL/6 mice were used as controls. Individual total RNA samples were derived from four retinas (pooled from two animals). All microarray experiments were performed in triplicate, in each case with separate RNA preparations. Microarray experiments with cDNAs were performed with the P0, P6, and P14 derived samples. Probes were labeled with either Cy3 or Cy5 using the Array 900 kit from Genisphere (Hatfield, Pennsylvania, United States) starting with 5 μg of total RNA according to the manufacturer's instructions. Wild-type control probes were compared to mutant on the same microarray. In two of the three replicates, the mutant probe was labeled with Cy3 and the wild type with Cy5, and in the third replicate the dyes were swapped. Labeled probe was hybridized to microarray slides spotted with approximately 11,500 cDNA clones from the brain molecular anatomy project library (kind gift of B. Soares, University of Iowa; see http://trans.nih.gov/bmap/index.htm for details) and 500 cDNA clones from our lab collection. Slides were printed and hybridized as described [59,60]. After hybridization and washing of slides according to the manufacturer's instructions (Genisphere), the slides were scanned on an Axon Instruments (Union City, California, United States) GenePix 4000 scanner and images were analyzed using the accompanying GenePix Pro software package. The complete raw cDNA microarray data set are available in Tables S6–S14."}