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Both studies showed that cone genes in addition to S-cone opsin are derepressed in the mouse rd7 mutant. In addition, Peng et al. [19] found by RT-PCR that the levels of several rod-specific genes, including rhodopsin, were modestly reduced in rd7 at P28. Our in situ hybridization data suggest that rhodopsin expression is markedly reduced at P6, but that it attains levels indistinguishable from wild-type by P14. Since the change in rhodopsin levels identified by Peng et al. were relatively small (an approximately 15% reduction), it is not surprising that such a difference was not detected by in situ hybridization. The overall finding of modest reductions in rod-specific gene expression is entirely in keeping with the results of the present study."}

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{"project":"craft-ca-core-dev","denotations":[{"id":"T7455","span":{"begin":132,"end":136},"obj":"CL:0000573"},{"id":"T7456","span":{"begin":137,"end":142},"obj":"SO:0000704"},{"id":"T7457","span":{"begin":158,"end":170},"obj":"PR:000001195"},{"id":"T7458","span":{"begin":160,"end":164},"obj":"CL:0000573"},{"id":"T7459","span":{"begin":194,"end":199},"obj":"NCBITaxon:10088"},{"id":"T7460","span":{"begin":285,"end":288},"obj":"CL:0000604"},{"id":"T7461","span":{"begin":298,"end":303},"obj":"SO:0000704"},{"id":"T7462","span":{"begin":315,"end":324},"obj":"PR:000001245"},{"id":"T7463","span":{"begin":375,"end":388},"obj":"GO:0097617"},{"id":"T7464","span":{"begin":407,"end":416},"obj":"PR:000001245"},{"id":"T7465","span":{"begin":417,"end":427},"obj":"GO:0010467"},{"id":"T7466","span":{"begin":543,"end":552},"obj":"PR:000001245"},{"id":"T7467","span":{"begin":714,"end":727},"obj":"GO:0097617"},{"id":"T7468","span":{"begin":773,"end":776},"obj":"CL:0000604"},{"id":"T7469","span":{"begin":786,"end":790},"obj":"SO:0000704"},{"id":"T7470","span":{"begin":786,"end":801},"obj":"GO:0010467"}],"text":"Two recent studies have presented data that are consistent with many of the findings in our study [18,19]. Both studies showed that cone genes in addition to S-cone opsin are derepressed in the mouse rd7 mutant. In addition, Peng et al. [19] found by RT-PCR that the levels of several rod-specific genes, including rhodopsin, were modestly reduced in rd7 at P28. Our in situ hybridization data suggest that rhodopsin expression is markedly reduced at P6, but that it attains levels indistinguishable from wild-type by P14. Since the change in rhodopsin levels identified by Peng et al. were relatively small (an approximately 15% reduction), it is not surprising that such a difference was not detected by in situ hybridization. The overall finding of modest reductions in rod-specific gene expression is entirely in keeping with the results of the present study."}

{"project":"2_test","denotations":[{"id":"16110338-15634773-85798532","span":{"begin":99,"end":101},"obj":"15634773"},{"id":"16110338-15689355-85798533","span":{"begin":102,"end":104},"obj":"15689355"},{"id":"16110338-15689355-85798534","span":{"begin":238,"end":240},"obj":"15689355"},{"id":"T30895","span":{"begin":99,"end":101},"obj":"15634773"},{"id":"T84637","span":{"begin":102,"end":104},"obj":"15689355"},{"id":"T69025","span":{"begin":238,"end":240},"obj":"15689355"}],"text":"Two recent studies have presented data that are consistent with many of the findings in our study [18,19]. Both studies showed that cone genes in addition to S-cone opsin are derepressed in the mouse rd7 mutant. In addition, Peng et al. [19] found by RT-PCR that the levels of several rod-specific genes, including rhodopsin, were modestly reduced in rd7 at P28. Our in situ hybridization data suggest that rhodopsin expression is markedly reduced at P6, but that it attains levels indistinguishable from wild-type by P14. Since the change in rhodopsin levels identified by Peng et al. were relatively small (an approximately 15% reduction), it is not surprising that such a difference was not detected by in situ hybridization. The overall finding of modest reductions in rod-specific gene expression is entirely in keeping with the results of the present study."}