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quantitative trait loci (QTL) have been identified that influence two-bottle intake of bitter stimuli in the mouse, including loci for quinine (Qui) [12,16,26], cyclohexamide (Cyx) [13] and sucrose octaacetate (Soa) [14,15,17] sensitivity. Each of these QTL map to mouse distal chromosome 6 and are linked to the marker D6Mit13, which lies within a cluster of 24 intact Tas2rs in the C57BL/6 genome (e.g., [16,27,28]). However, the interpretation of these studies remains problematic for two reasons. First, the density of chromosomal markers and number of recombinant inbred (RI) strains used in these earlier studies did not permit the physical definition of the intervals containing each QTL. Second, these previous attempts to map bitter taste QTLs relied on behavioral assays that measured consumption, and were thus susceptible to contributions of post-ingestive effects such as toxicity. As we have shown previously, such effects can confound the quantification of bitter taste behaviors [29]. Therefore, the relevance and/or contribution of the aforementioned QTLs to bitter taste remain unclear."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T652","span":{"begin":8,"end":31},"obj":"SO_EXT:0000771"},{"id":"T653","span":{"begin":33,"end":36},"obj":"SO_EXT:0000771"},{"id":"T654","span":{"begin":85,"end":91},"obj":"GO:0007631"},{"id":"T655","span":{"begin":117,"end":122},"obj":"NCBITaxon:10088"},{"id":"T656","span":{"begin":143,"end":150},"obj":"CHEBI:15854"},{"id":"T657","span":{"begin":152,"end":155},"obj":"CHEBI:15854"},{"id":"T658","span":{"begin":169,"end":182},"obj":"CHEBI:27641"},{"id":"T659","span":{"begin":184,"end":187},"obj":"CHEBI:27641"},{"id":"T660","span":{"begin":262,"end":265},"obj":"SO_EXT:0000771"},{"id":"T661","span":{"begin":273,"end":278},"obj":"NCBITaxon:10088"},{"id":"T662","span":{"begin":286,"end":296},"obj":"GO_SO_EXT:chromosome"},{"id":"T663","span":{"begin":321,"end":327},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T664","span":{"begin":378,"end":384},"obj":"PR_EXT:000036337"},{"id":"T665","span":{"begin":400,"end":406},"obj":"SO_EXT:0001026"},{"id":"T666","span":{"begin":531,"end":542},"obj":"GO_SO_EXT:chromosome"},{"id":"T667","span":{"begin":543,"end":550},"obj":"CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag"},{"id":"T668","span":{"begin":565,"end":576},"obj":"GO_SO_EXT:sequence_rearrangement_process"},{"id":"T669","span":{"begin":699,"end":702},"obj":"SO_EXT:0000771"},{"id":"T670","span":{"begin":743,"end":755},"obj":"GO:0050913"},{"id":"T671","span":{"begin":756,"end":760},"obj":"SO_EXT:0000771"},{"id":"T672","span":{"begin":771,"end":781},"obj":"GO_PATO_EXT:biological_behavior"},{"id":"T673","span":{"begin":803,"end":814},"obj":"GO:0007631"},{"id":"T674","span":{"begin":867,"end":876},"obj":"GO:0007631"},{"id":"T675","span":{"begin":980,"end":992},"obj":"GO:0050913"},{"id":"T676","span":{"begin":993,"end":1002},"obj":"GO_PATO_EXT:biological_behavior"},{"id":"T677","span":{"begin":1076,"end":1080},"obj":"SO_EXT:0000771"},{"id":"T678","span":{"begin":1084,"end":1096},"obj":"GO:0050913"}],"text":"Several quantitative trait loci (QTL) have been identified that influence two-bottle intake of bitter stimuli in the mouse, including loci for quinine (Qui) [12,16,26], cyclohexamide (Cyx) [13] and sucrose octaacetate (Soa) [14,15,17] sensitivity. Each of these QTL map to mouse distal chromosome 6 and are linked to the marker D6Mit13, which lies within a cluster of 24 intact Tas2rs in the C57BL/6 genome (e.g., [16,27,28]). However, the interpretation of these studies remains problematic for two reasons. First, the density of chromosomal markers and number of recombinant inbred (RI) strains used in these earlier studies did not permit the physical definition of the intervals containing each QTL. Second, these previous attempts to map bitter taste QTLs relied on behavioral assays that measured consumption, and were thus susceptible to contributions of post-ingestive effects such as toxicity. As we have shown previously, such effects can confound the quantification of bitter taste behaviors [29]. Therefore, the relevance and/or contribution of the aforementioned QTLs to bitter taste remain unclear."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T544","span":{"begin":8,"end":31},"obj":"SO:0000771"},{"id":"T545","span":{"begin":33,"end":36},"obj":"SO:0000771"},{"id":"T546","span":{"begin":85,"end":91},"obj":"GO:0007631"},{"id":"T547","span":{"begin":117,"end":122},"obj":"NCBITaxon:10088"},{"id":"T548","span":{"begin":143,"end":150},"obj":"CHEBI:15854"},{"id":"T549","span":{"begin":152,"end":155},"obj":"CHEBI:15854"},{"id":"T550","span":{"begin":169,"end":182},"obj":"CHEBI:27641"},{"id":"T551","span":{"begin":184,"end":187},"obj":"CHEBI:27641"},{"id":"T552","span":{"begin":262,"end":265},"obj":"SO:0000771"},{"id":"T553","span":{"begin":273,"end":278},"obj":"NCBITaxon:10088"},{"id":"T554","span":{"begin":378,"end":384},"obj":"PR:000036337"},{"id":"T555","span":{"begin":400,"end":406},"obj":"SO:0001026"},{"id":"T556","span":{"begin":699,"end":702},"obj":"SO:0000771"},{"id":"T557","span":{"begin":743,"end":755},"obj":"GO:0050913"},{"id":"T558","span":{"begin":756,"end":760},"obj":"SO:0000771"},{"id":"T559","span":{"begin":803,"end":814},"obj":"GO:0007631"},{"id":"T560","span":{"begin":867,"end":876},"obj":"GO:0007631"},{"id":"T561","span":{"begin":980,"end":992},"obj":"GO:0050913"},{"id":"T562","span":{"begin":1076,"end":1080},"obj":"SO:0000771"},{"id":"T563","span":{"begin":1084,"end":1096},"obj":"GO:0050913"}],"text":"Several quantitative trait loci (QTL) have been identified that influence two-bottle intake of bitter stimuli in the mouse, including loci for quinine (Qui) [12,16,26], cyclohexamide (Cyx) [13] and sucrose octaacetate (Soa) [14,15,17] sensitivity. Each of these QTL map to mouse distal chromosome 6 and are linked to the marker D6Mit13, which lies within a cluster of 24 intact Tas2rs in the C57BL/6 genome (e.g., [16,27,28]). However, the interpretation of these studies remains problematic for two reasons. First, the density of chromosomal markers and number of recombinant inbred (RI) strains used in these earlier studies did not permit the physical definition of the intervals containing each QTL. Second, these previous attempts to map bitter taste QTLs relied on behavioral assays that measured consumption, and were thus susceptible to contributions of post-ingestive effects such as toxicity. As we have shown previously, such effects can confound the quantification of bitter taste behaviors [29]. Therefore, the relevance and/or contribution of the aforementioned QTLs to bitter taste remain unclear."}