Id |
Subject |
Object |
Predicate |
Lexical cue |
T294 |
0-2 |
IN |
denotes |
In |
T295 |
22-26 |
VBN |
denotes |
used |
T296 |
3-7 |
DT |
denotes |
this |
T297 |
8-13 |
NN |
denotes |
study |
T298 |
14-16 |
PRP |
denotes |
we |
T299 |
17-21 |
VBP |
denotes |
have |
T300 |
27-36 |
JJ |
denotes |
embryonic |
T301 |
37-41 |
NN |
denotes |
stem |
T302 |
47-51 |
NN |
denotes |
cell |
T303 |
42-43 |
-LRB- |
denotes |
( |
T304 |
43-45 |
NN |
denotes |
ES |
T305 |
45-46 |
-RRB- |
denotes |
) |
T306 |
52-60 |
VBN |
denotes |
mediated |
T307 |
51-52 |
HYPH |
denotes |
- |
T308 |
61-73 |
NN |
denotes |
transgenesis |
T309 |
74-76 |
TO |
denotes |
to |
T310 |
77-81 |
VB |
denotes |
test |
T311 |
82-85 |
DT |
denotes |
the |
T312 |
112-119 |
NN |
denotes |
protein |
T313 |
86-94 |
VBN |
denotes |
enhanced |
T314 |
95-99 |
NN |
denotes |
cyan |
T315 |
100-111 |
JJ |
denotes |
fluorescent |
T316 |
120-121 |
-LRB- |
denotes |
( |
T317 |
121-125 |
NN |
denotes |
ECFP |
T318 |
125-126 |
-RRB- |
denotes |
) |
T319 |
127-130 |
CC |
denotes |
and |
T320 |
131-139 |
VBN |
denotes |
enhanced |
T321 |
159-166 |
NN |
denotes |
protein |
T322 |
140-146 |
JJ |
denotes |
yellow |
T323 |
147-158 |
JJ |
denotes |
fluorescent |
T324 |
167-168 |
-LRB- |
denotes |
( |
T325 |
168-172 |
NN |
denotes |
EYFP |
T326 |
172-173 |
-RRB- |
denotes |
) |
T327 |
173-175 |
, |
denotes |
, |
T328 |
175-178 |
CD |
denotes |
two |
T329 |
216-224 |
NNS |
denotes |
variants |
T330 |
179-185 |
JJ |
denotes |
mutant |
T331 |
186-189 |
CC |
denotes |
and |
T332 |
190-200 |
RB |
denotes |
spectrally |
T333 |
201-209 |
JJ |
denotes |
distinct |
T334 |
210-215 |
NN |
denotes |
color |
T335 |
225-227 |
IN |
denotes |
of |
T336 |
228-232 |
JJ |
denotes |
wild |
T337 |
233-237 |
NN |
denotes |
type |
T338 |
243-246 |
NN |
denotes |
GFP |
T339 |
238-239 |
-LRB- |
denotes |
( |
T340 |
239-241 |
NN |
denotes |
wt |
T341 |
241-242 |
-RRB- |
denotes |
) |
T342 |
246-247 |
. |
denotes |
. |
T343 |
247-394 |
sentence |
denotes |
We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. |
T344 |
248-250 |
PRP |
denotes |
We |
T345 |
261-267 |
VBN |
denotes |
tested |
T346 |
251-255 |
VBP |
denotes |
have |
T347 |
256-260 |
RB |
denotes |
also |
T348 |
268-274 |
NN |
denotes |
DsRed1 |
T349 |
274-276 |
, |
denotes |
, |
T350 |
276-279 |
DT |
denotes |
the |
T351 |
310-318 |
NN |
denotes |
reporter |
T352 |
280-285 |
JJ |
denotes |
novel |
T353 |
286-289 |
JJ |
denotes |
red |
T354 |
302-309 |
NN |
denotes |
protein |
T355 |
290-301 |
JJ |
denotes |
fluorescent |
T356 |
319-327 |
RB |
denotes |
recently |
T357 |
328-334 |
VBN |
denotes |
cloned |
T358 |
335-339 |
IN |
denotes |
from |
T359 |
340-343 |
DT |
denotes |
the |
T360 |
355-360 |
NN |
denotes |
coral |
T361 |
344-354 |
NN |
denotes |
Discostoma |
T362 |
361-363 |
IN |
denotes |
by |
T363 |
364-370 |
NN |
denotes |
virtue |
T364 |
371-373 |
IN |
denotes |
of |
T365 |
374-377 |
PRP$ |
denotes |
its |
T366 |
378-386 |
NN |
denotes |
homology |
T367 |
387-389 |
IN |
denotes |
to |
T368 |
390-393 |
NN |
denotes |
GFP |
T369 |
393-394 |
. |
denotes |
. |
T370 |
394-562 |
sentence |
denotes |
To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. |
T371 |
395-397 |
IN |
denotes |
To |
T372 |
416-427 |
VBN |
denotes |
established |
T373 |
398-402 |
DT |
denotes |
this |
T374 |
403-406 |
NN |
denotes |
end |
T375 |
406-408 |
, |
denotes |
, |
T376 |
408-410 |
PRP |
denotes |
we |
T377 |
411-415 |
VBP |
denotes |
have |
T378 |
428-433 |
NNS |
denotes |
lines |
T379 |
434-436 |
IN |
denotes |
of |
T380 |
437-439 |
NN |
denotes |
ES |
T381 |
440-445 |
NNS |
denotes |
cells |
T382 |
446-454 |
RB |
denotes |
together |
T383 |
455-459 |
IN |
denotes |
with |
T384 |
460-466 |
JJ |
denotes |
viable |
T385 |
479-483 |
NNS |
denotes |
mice |
T386 |
467-470 |
CC |
denotes |
and |
T387 |
471-478 |
JJ |
denotes |
fertile |
T388 |
484-490 |
VBG |
denotes |
having |
T389 |
491-501 |
JJ |
denotes |
widespread |
T390 |
502-512 |
NN |
denotes |
expression |
T391 |
513-515 |
IN |
denotes |
of |
T392 |
516-522 |
CC |
denotes |
either |
T393 |
552-561 |
NNS |
denotes |
reporters |
T394 |
523-526 |
DT |
denotes |
the |
T395 |
527-531 |
NN |
denotes |
ECFP |
T396 |
532-534 |
CC |
denotes |
or |
T397 |
535-539 |
NN |
denotes |
EYFP |
T398 |
540-543 |
NN |
denotes |
GFP |
T399 |
544-551 |
NN |
denotes |
variant |
T400 |
543-544 |
HYPH |
denotes |
- |
T401 |
561-562 |
. |
denotes |
. |
T402 |
562-742 |
sentence |
denotes |
However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively. |
T403 |
563-570 |
RB |
denotes |
However |
T404 |
575-579 |
VBD |
denotes |
were |
T405 |
570-572 |
, |
denotes |
, |
T406 |
572-574 |
PRP |
denotes |
we |
T407 |
580-586 |
JJ |
denotes |
unable |
T408 |
587-589 |
TO |
denotes |
to |
T409 |
590-598 |
VB |
denotes |
generate |
T410 |
599-609 |
JJ |
denotes |
equivalent |
T411 |
617-622 |
NNS |
denotes |
lines |
T412 |
610-616 |
NN |
denotes |
DsRed1 |
T413 |
622-624 |
, |
denotes |
, |
T414 |
624-634 |
VBG |
denotes |
suggesting |
T415 |
635-639 |
IN |
denotes |
that |
T416 |
647-649 |
VBZ |
denotes |
is |
T417 |
640-646 |
NN |
denotes |
DsRed1 |
T418 |
650-653 |
RB |
denotes |
not |
T419 |
654-669 |
RB |
denotes |
developmentally |
T420 |
670-677 |
JJ |
denotes |
neutral |
T421 |
678-680 |
CC |
denotes |
or |
T422 |
681-685 |
IN |
denotes |
that |
T423 |
717-726 |
VBN |
denotes |
sustained |
T424 |
686-695 |
NN |
denotes |
transgene |
T425 |
696-706 |
NN |
denotes |
expression |
T426 |
707-710 |
MD |
denotes |
can |
T427 |
710-713 |
RB |
denotes |
not |
T428 |
714-716 |
VB |
denotes |
be |
T429 |
727-741 |
RB |
denotes |
constitutively |
T430 |
741-742 |
. |
denotes |
. |
T431 |
742-1010 |
sentence |
denotes |
Balanced (diploid <-> diploid) and polarized (tetraploid <-> diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal. |
T432 |
743-751 |
JJ |
denotes |
Balanced |
T433 |
813-821 |
NNS |
denotes |
chimeras |
T434 |
752-753 |
-LRB- |
denotes |
( |
T435 |
765-772 |
JJ |
denotes |
diploid |
T436 |
753-760 |
JJ |
denotes |
diploid |
T437 |
761-762 |
SYM |
denotes |
< |
T438 |
762-763 |
HYPH |
denotes |
- |
T439 |
763-764 |
SYM |
denotes |
> |
T440 |
772-773 |
-RRB- |
denotes |
) |
T441 |
774-777 |
CC |
denotes |
and |
T442 |
778-787 |
JJ |
denotes |
polarized |
T443 |
788-789 |
-LRB- |
denotes |
( |
T444 |
804-811 |
JJ |
denotes |
diploid |
T445 |
789-799 |
JJ |
denotes |
tetraploid |
T446 |
800-801 |
SYM |
denotes |
< |
T447 |
801-802 |
HYPH |
denotes |
- |
T448 |
802-803 |
SYM |
denotes |
> |
T449 |
811-812 |
-RRB- |
denotes |
) |
T450 |
892-903 |
VBP |
denotes |
demonstrate |
T451 |
822-832 |
VBG |
denotes |
comprising |
T452 |
833-845 |
NNS |
denotes |
combinations |
T453 |
846-848 |
IN |
denotes |
of |
T454 |
849-852 |
DT |
denotes |
the |
T455 |
853-857 |
NN |
denotes |
ECFP |
T456 |
858-861 |
CC |
denotes |
and |
T457 |
862-866 |
NN |
denotes |
EYFP |
T458 |
867-869 |
NN |
denotes |
ES |
T459 |
870-875 |
NNS |
denotes |
cells |
T460 |
876-879 |
CC |
denotes |
and |
T461 |
879-880 |
HYPH |
denotes |
/ |
T462 |
880-882 |
CC |
denotes |
or |
T463 |
883-890 |
NNS |
denotes |
embryos |
T464 |
890-892 |
, |
denotes |
, |
T465 |
904-908 |
IN |
denotes |
that |
T466 |
973-986 |
VBN |
denotes |
distinguished |
T467 |
909-920 |
NNS |
denotes |
populations |
T468 |
921-923 |
IN |
denotes |
of |
T469 |
924-929 |
NNS |
denotes |
cells |
T470 |
930-940 |
VBG |
denotes |
expressing |
T471 |
941-945 |
DT |
denotes |
each |
T472 |
957-965 |
NN |
denotes |
reporter |
T473 |
946-956 |
JJ |
denotes |
individual |
T474 |
966-969 |
MD |
denotes |
can |
T475 |
970-972 |
VB |
denotes |
be |
T476 |
987-993 |
IN |
denotes |
within |
T477 |
994-995 |
DT |
denotes |
a |
T478 |
1003-1009 |
NN |
denotes |
animal |
T479 |
996-1002 |
JJ |
denotes |
single |
T480 |
1009-1010 |
. |
denotes |
. |
R129 |
T294 |
T295 |
prep |
In,used |
R130 |
T296 |
T297 |
det |
this,study |
R131 |
T297 |
T294 |
pobj |
study,In |
R132 |
T298 |
T295 |
nsubj |
we,used |
R133 |
T299 |
T295 |
aux |
have,used |
R134 |
T300 |
T301 |
amod |
embryonic,stem |
R135 |
T301 |
T302 |
nmod |
stem,cell |
R136 |
T302 |
T306 |
npadvmod |
cell,mediated |
R137 |
T303 |
T301 |
punct |
(,stem |
R138 |
T304 |
T301 |
appos |
ES,stem |
R139 |
T305 |
T302 |
punct |
),cell |
R140 |
T306 |
T308 |
amod |
mediated,transgenesis |
R141 |
T307 |
T306 |
punct |
-,mediated |
R142 |
T308 |
T295 |
dobj |
transgenesis,used |
R143 |
T309 |
T310 |
aux |
to,test |
R144 |
T310 |
T295 |
advcl |
test,used |
R145 |
T311 |
T312 |
det |
the,protein |
R146 |
T312 |
T310 |
dobj |
protein,test |
R147 |
T313 |
T312 |
amod |
enhanced,protein |
R148 |
T314 |
T312 |
nmod |
cyan,protein |
R149 |
T315 |
T312 |
amod |
fluorescent,protein |
R150 |
T316 |
T312 |
punct |
(,protein |
R151 |
T317 |
T312 |
appos |
ECFP,protein |
R152 |
T318 |
T312 |
punct |
),protein |
R153 |
T319 |
T312 |
cc |
and,protein |
R154 |
T320 |
T321 |
amod |
enhanced,protein |
R155 |
T321 |
T312 |
conj |
protein,protein |
R156 |
T322 |
T321 |
amod |
yellow,protein |
R157 |
T323 |
T321 |
amod |
fluorescent,protein |
R158 |
T324 |
T321 |
punct |
(,protein |
R159 |
T325 |
T321 |
appos |
EYFP,protein |
R160 |
T326 |
T312 |
punct |
),protein |
R161 |
T327 |
T312 |
punct |
", ",protein |
R162 |
T328 |
T329 |
nummod |
two,variants |
R163 |
T329 |
T312 |
appos |
variants,protein |
R164 |
T330 |
T329 |
amod |
mutant,variants |
R165 |
T331 |
T330 |
cc |
and,mutant |
R166 |
T332 |
T333 |
advmod |
spectrally,distinct |
R167 |
T333 |
T330 |
conj |
distinct,mutant |
R168 |
T334 |
T329 |
compound |
color,variants |
R169 |
T335 |
T329 |
prep |
of,variants |
R170 |
T336 |
T337 |
amod |
wild,type |
R171 |
T337 |
T338 |
nmod |
type,GFP |
R172 |
T338 |
T335 |
pobj |
GFP,of |
R173 |
T339 |
T337 |
punct |
(,type |
R174 |
T340 |
T337 |
appos |
wt,type |
R175 |
T341 |
T338 |
punct |
),GFP |
R176 |
T342 |
T295 |
punct |
.,used |
R177 |
T344 |
T345 |
nsubj |
We,tested |
R178 |
T346 |
T345 |
aux |
have,tested |
R179 |
T347 |
T345 |
advmod |
also,tested |
R180 |
T348 |
T345 |
dobj |
DsRed1,tested |
R181 |
T349 |
T348 |
punct |
", ",DsRed1 |
R182 |
T350 |
T351 |
det |
the,reporter |
R183 |
T351 |
T348 |
appos |
reporter,DsRed1 |
R184 |
T352 |
T351 |
amod |
novel,reporter |
R185 |
T353 |
T354 |
amod |
red,protein |
R186 |
T354 |
T351 |
compound |
protein,reporter |
R187 |
T355 |
T354 |
amod |
fluorescent,protein |
R188 |
T356 |
T357 |
advmod |
recently,cloned |
R189 |
T357 |
T351 |
acl |
cloned,reporter |
R190 |
T358 |
T357 |
prep |
from,cloned |
R191 |
T359 |
T360 |
det |
the,coral |
R192 |
T360 |
T358 |
pobj |
coral,from |
R193 |
T361 |
T360 |
compound |
Discostoma,coral |
R194 |
T362 |
T357 |
prep |
by,cloned |
R195 |
T363 |
T362 |
pobj |
virtue,by |
R196 |
T364 |
T363 |
prep |
of,virtue |
R197 |
T365 |
T366 |
poss |
its,homology |
R198 |
T366 |
T364 |
pobj |
homology,of |
R199 |
T367 |
T366 |
prep |
to,homology |
R200 |
T368 |
T367 |
pobj |
GFP,to |
R201 |
T369 |
T345 |
punct |
.,tested |
R202 |
T371 |
T372 |
prep |
To,established |
R203 |
T373 |
T374 |
det |
this,end |
R204 |
T374 |
T371 |
pobj |
end,To |
R205 |
T375 |
T372 |
punct |
", ",established |
R206 |
T376 |
T372 |
nsubj |
we,established |
R207 |
T377 |
T372 |
aux |
have,established |
R208 |
T378 |
T372 |
dobj |
lines,established |
R209 |
T379 |
T378 |
prep |
of,lines |
R210 |
T380 |
T381 |
compound |
ES,cells |
R211 |
T381 |
T379 |
pobj |
cells,of |
R212 |
T382 |
T383 |
advmod |
together,with |
R213 |
T383 |
T372 |
prep |
with,established |
R214 |
T384 |
T385 |
amod |
viable,mice |
R215 |
T385 |
T383 |
pobj |
mice,with |
R216 |
T386 |
T384 |
cc |
and,viable |
R217 |
T387 |
T384 |
conj |
fertile,viable |
R218 |
T388 |
T385 |
acl |
having,mice |
R219 |
T389 |
T390 |
amod |
widespread,expression |
R220 |
T390 |
T388 |
dobj |
expression,having |
R221 |
T391 |
T390 |
prep |
of,expression |
R222 |
T392 |
T393 |
preconj |
either,reporters |
R224 |
T394 |
T393 |
det |
the,reporters |
R225 |
T395 |
T393 |
nmod |
ECFP,reporters |
R226 |
T396 |
T395 |
cc |
or,ECFP |
R227 |
T397 |
T395 |
conj |
EYFP,ECFP |
R228 |
T398 |
T399 |
compound |
GFP,variant |
R229 |
T399 |
T393 |
compound |
variant,reporters |
R230 |
T400 |
T399 |
punct |
-,variant |
R231 |
T401 |
T372 |
punct |
.,established |
R232 |
T403 |
T404 |
advmod |
However,were |
R233 |
T405 |
T404 |
punct |
", ",were |
R234 |
T406 |
T404 |
nsubj |
we,were |
R235 |
T407 |
T404 |
acomp |
unable,were |
R236 |
T408 |
T409 |
aux |
to,generate |
R237 |
T409 |
T407 |
xcomp |
generate,unable |
R238 |
T410 |
T411 |
amod |
equivalent,lines |
R239 |
T411 |
T409 |
dobj |
lines,generate |
R240 |
T412 |
T411 |
compound |
DsRed1,lines |
R241 |
T413 |
T404 |
punct |
", ",were |
R242 |
T414 |
T404 |
advcl |
suggesting,were |
R243 |
T415 |
T416 |
mark |
that,is |
R244 |
T416 |
T414 |
advcl |
is,suggesting |
R245 |
T417 |
T416 |
nsubj |
DsRed1,is |
R246 |
T418 |
T416 |
neg |
not,is |
R247 |
T419 |
T420 |
advmod |
developmentally,neutral |
R248 |
T420 |
T416 |
acomp |
neutral,is |
R249 |
T421 |
T416 |
cc |
or,is |
R250 |
T422 |
T423 |
mark |
that,sustained |
R251 |
T423 |
T416 |
conj |
sustained,is |
R252 |
T424 |
T425 |
compound |
transgene,expression |
R253 |
T425 |
T423 |
nsubjpass |
expression,sustained |
R254 |
T426 |
T423 |
aux |
can,sustained |
R255 |
T427 |
T423 |
neg |
not,sustained |
R256 |
T428 |
T423 |
auxpass |
be,sustained |
R257 |
T429 |
T423 |
advmod |
constitutively,sustained |
R258 |
T430 |
T404 |
punct |
.,were |
R259 |
T432 |
T433 |
amod |
Balanced,chimeras |
R260 |
T433 |
T450 |
nsubj |
chimeras,demonstrate |
R261 |
T434 |
T435 |
punct |
(,diploid |
R262 |
T435 |
T432 |
parataxis |
diploid,Balanced |
R263 |
T436 |
T435 |
nsubj |
diploid,diploid |
R264 |
T437 |
T435 |
punct |
<,diploid |
R265 |
T438 |
T435 |
punct |
-,diploid |
R266 |
T439 |
T435 |
punct |
>,diploid |
R267 |
T440 |
T435 |
punct |
),diploid |
R268 |
T441 |
T432 |
cc |
and,Balanced |
R269 |
T442 |
T432 |
conj |
polarized,Balanced |
R270 |
T443 |
T444 |
punct |
(,diploid |
R271 |
T444 |
T442 |
parataxis |
diploid,polarized |
R272 |
T445 |
T444 |
nsubj |
tetraploid,diploid |
R273 |
T446 |
T444 |
punct |
<,diploid |
R274 |
T447 |
T444 |
punct |
-,diploid |
R275 |
T448 |
T444 |
punct |
>,diploid |
R276 |
T449 |
T444 |
punct |
),diploid |
R277 |
T451 |
T433 |
acl |
comprising,chimeras |
R278 |
T452 |
T451 |
dobj |
combinations,comprising |
R279 |
T453 |
T452 |
prep |
of,combinations |
R280 |
T454 |
T455 |
det |
the,ECFP |
R281 |
T455 |
T453 |
pobj |
ECFP,of |
R282 |
T456 |
T455 |
cc |
and,ECFP |
R283 |
T457 |
T455 |
conj |
EYFP,ECFP |
R284 |
T458 |
T459 |
compound |
ES,cells |
R285 |
T459 |
T455 |
appos |
cells,ECFP |
R286 |
T460 |
T459 |
cc |
and,cells |
R287 |
T461 |
T460 |
punct |
/,and |
R288 |
T462 |
T460 |
cc |
or,and |
R289 |
T463 |
T459 |
conj |
embryos,cells |
R290 |
T464 |
T450 |
punct |
", ",demonstrate |
R291 |
T465 |
T466 |
mark |
that,distinguished |
R292 |
T466 |
T450 |
ccomp |
distinguished,demonstrate |
R293 |
T467 |
T466 |
nsubjpass |
populations,distinguished |
R294 |
T468 |
T467 |
prep |
of,populations |
R295 |
T469 |
T468 |
pobj |
cells,of |
R296 |
T470 |
T467 |
acl |
expressing,populations |
R297 |
T471 |
T472 |
det |
each,reporter |
R298 |
T472 |
T470 |
dobj |
reporter,expressing |
R299 |
T473 |
T472 |
amod |
individual,reporter |
R300 |
T474 |
T466 |
aux |
can,distinguished |
R301 |
T475 |
T466 |
auxpass |
be,distinguished |
R302 |
T476 |
T466 |
prep |
within,distinguished |
R303 |
T477 |
T478 |
det |
a,animal |
R304 |
T478 |
T476 |
pobj |
animal,within |
R305 |
T479 |
T478 |
amod |
single,animal |
R306 |
T480 |
T450 |
punct |
.,demonstrate |
R223 |
T393 |
T391 |
pobj |
reporters,of |