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We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively. Balanced (diploid \u003c-\u003e diploid) and polarized (tetraploid \u003c-\u003e diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal."}

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{"project":"craft-ca-core-dev","denotations":[{"id":"T71","span":{"begin":27,"end":36},"obj":"UBERON:0000922"},{"id":"T72","span":{"begin":27,"end":41},"obj":"_FRAGMENT"},{"id":"T73","span":{"begin":47,"end":51},"obj":"CL:0002322"},{"id":"T74","span":{"begin":43,"end":45},"obj":"_FRAGMENT"},{"id":"T75","span":{"begin":344,"end":354},"obj":"NCBITaxon:86599"},{"id":"T76","span":{"begin":378,"end":386},"obj":"SO:0000857"},{"id":"T77","span":{"begin":437,"end":445},"obj":"CL:0002322"},{"id":"T78","span":{"begin":479,"end":483},"obj":"NCBITaxon:10088"},{"id":"T79","span":{"begin":502,"end":512},"obj":"GO:0010467"},{"id":"T80","span":{"begin":686,"end":695},"obj":"SO:0000902"},{"id":"T81","span":{"begin":696,"end":706},"obj":"GO:0010467"},{"id":"T82","span":{"begin":867,"end":875},"obj":"CL:0002322"},{"id":"T83","span":{"begin":883,"end":890},"obj":"UBERON:0000922"},{"id":"T84","span":{"begin":930,"end":940},"obj":"GO:0010467"},{"id":"T85","span":{"begin":1003,"end":1009},"obj":"NCBITaxon:33208"}],"relations":[{"id":"R3","pred":"_lexicallyChainedTo","subj":"T73","obj":"T72"},{"id":"R4","pred":"_lexicallyChainedTo","subj":"T73","obj":"T74"}],"text":"In this study we have used embryonic stem (ES) cell-mediated transgenesis to test the enhanced cyan fluorescent protein (ECFP) and enhanced yellow fluorescent protein (EYFP), two mutant and spectrally distinct color variants of wild type (wt) GFP. We have also tested DsRed1, the novel red fluorescent protein reporter recently cloned from the Discostoma coral by virtue of its homology to GFP. To this end, we have established lines of ES cells together with viable and fertile mice having widespread expression of either the ECFP or EYFP GFP-variant reporters. However, we were unable to generate equivalent DsRed1 lines, suggesting that DsRed1 is not developmentally neutral or that transgene expression cannot be sustained constitutively. Balanced (diploid \u003c-\u003e diploid) and polarized (tetraploid \u003c-\u003e diploid) chimeras comprising combinations of the ECFP and EYFP ES cells and/or embryos, demonstrate that populations of cells expressing each individual reporter can be distinguished within a single animal."}