Id |
Subject |
Object |
Predicate |
Lexical cue |
T1679 |
0-3 |
DT |
denotes |
The |
T1680 |
16-29 |
NNS |
denotes |
baculoviruses |
T1681 |
4-15 |
JJ |
denotes |
recombinant |
T1682 |
94-103 |
VBN |
denotes |
described |
T1683 |
30-40 |
VBG |
denotes |
expressing |
T1684 |
41-45 |
NN |
denotes |
His6 |
T1685 |
51-55 |
NN |
denotes |
Mcm6 |
T1686 |
45-46 |
HYPH |
denotes |
- |
T1687 |
46-50 |
NN |
denotes |
Mcm4 |
T1688 |
50-51 |
HYPH |
denotes |
/ |
T1689 |
69-77 |
NN |
denotes |
proteins |
T1690 |
56-58 |
CC |
denotes |
or |
T1691 |
59-63 |
NN |
denotes |
Mcm7 |
T1692 |
64-68 |
NN |
denotes |
FLAG |
T1693 |
63-64 |
HYPH |
denotes |
- |
T1694 |
78-82 |
VBD |
denotes |
were |
T1695 |
83-93 |
RB |
denotes |
previously |
T1696 |
104-105 |
-LRB- |
denotes |
( |
T1697 |
105-107 |
CD |
denotes |
11 |
T1698 |
107-108 |
-RRB- |
denotes |
) |
T1699 |
108-109 |
. |
denotes |
. |
T1700 |
109-261 |
sentence |
denotes |
Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. |
T1701 |
110-113 |
NN |
denotes |
Sf9 |
T1702 |
135-140 |
NNS |
denotes |
cells |
T1703 |
114-117 |
CC |
denotes |
and |
T1704 |
118-122 |
JJ |
denotes |
High |
T1705 |
123-127 |
CD |
denotes |
five |
T1706 |
128-134 |
NN |
denotes |
insect |
T1707 |
146-154 |
VBN |
denotes |
cultured |
T1708 |
141-145 |
VBD |
denotes |
were |
T1709 |
155-157 |
IN |
denotes |
at |
T1710 |
158-160 |
CD |
denotes |
27 |
T1711 |
160-162 |
NN |
denotes |
°C |
T1712 |
163-165 |
IN |
denotes |
in |
T1713 |
166-168 |
NN |
denotes |
Sf |
T1714 |
176-179 |
NN |
denotes |
SFM |
T1715 |
168-169 |
HYPH |
denotes |
- |
T1716 |
169-172 |
CD |
denotes |
900 |
T1717 |
173-175 |
CD |
denotes |
II |
T1718 |
180-181 |
-LRB- |
denotes |
( |
T1719 |
200-204 |
NNP |
denotes |
Inc. |
T1720 |
181-185 |
NNP |
denotes |
Life |
T1721 |
186-198 |
NNP |
denotes |
Technologies |
T1722 |
198-200 |
, |
denotes |
, |
T1723 |
204-205 |
-RRB- |
denotes |
) |
T1724 |
206-209 |
CC |
denotes |
and |
T1725 |
210-212 |
NN |
denotes |
EX |
T1726 |
213-217 |
NN |
denotes |
CELL |
T1727 |
212-213 |
HYPH |
denotes |
- |
T1728 |
222-228 |
NN |
denotes |
medium |
T1729 |
218-221 |
CD |
denotes |
405 |
T1730 |
229-230 |
-LRB- |
denotes |
( |
T1731 |
234-245 |
NNP |
denotes |
Biosciences |
T1732 |
230-233 |
NNP |
denotes |
JRH |
T1733 |
245-246 |
-RRB- |
denotes |
) |
T1734 |
246-248 |
, |
denotes |
, |
T1735 |
248-260 |
RB |
denotes |
respectively |
T1736 |
260-261 |
. |
denotes |
. |
T1737 |
261-483 |
sentence |
denotes |
For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. |
T1738 |
262-265 |
IN |
denotes |
For |
T1739 |
324-334 |
VBN |
denotes |
coinfected |
T1740 |
266-276 |
NN |
denotes |
expression |
T1741 |
277-279 |
IN |
denotes |
of |
T1742 |
280-283 |
DT |
denotes |
the |
T1743 |
293-301 |
NN |
denotes |
proteins |
T1744 |
284-288 |
NN |
denotes |
Mcm4 |
T1745 |
288-289 |
HYPH |
denotes |
/ |
T1746 |
289-290 |
CD |
denotes |
6 |
T1747 |
290-291 |
HYPH |
denotes |
/ |
T1748 |
291-292 |
CD |
denotes |
7 |
T1749 |
301-303 |
, |
denotes |
, |
T1750 |
303-307 |
JJ |
denotes |
High |
T1751 |
308-312 |
CD |
denotes |
five |
T1752 |
313-318 |
NNS |
denotes |
cells |
T1753 |
319-323 |
VBD |
denotes |
were |
T1754 |
335-339 |
IN |
denotes |
with |
T1755 |
340-351 |
JJ |
denotes |
recombinant |
T1756 |
352-365 |
NNS |
denotes |
baculoviruses |
T1757 |
366-376 |
VBG |
denotes |
expressing |
T1758 |
377-380 |
DT |
denotes |
the |
T1759 |
396-404 |
NN |
denotes |
proteins |
T1760 |
381-385 |
NN |
denotes |
His6 |
T1761 |
391-395 |
NN |
denotes |
Mcm6 |
T1762 |
385-386 |
HYPH |
denotes |
- |
T1763 |
386-390 |
NN |
denotes |
Mcm4 |
T1764 |
390-391 |
HYPH |
denotes |
/ |
T1765 |
405-408 |
CC |
denotes |
and |
T1766 |
409-414 |
DT |
denotes |
those |
T1767 |
415-425 |
VBG |
denotes |
expressing |
T1768 |
426-429 |
DT |
denotes |
the |
T1769 |
435-439 |
NN |
denotes |
FLAG |
T1770 |
430-434 |
NN |
denotes |
Mcm7 |
T1771 |
434-435 |
HYPH |
denotes |
- |
T1772 |
439-441 |
, |
denotes |
, |
T1773 |
441-444 |
CC |
denotes |
and |
T1774 |
445-449 |
VBD |
denotes |
were |
T1775 |
450-459 |
VBN |
denotes |
collected |
T1776 |
460-462 |
IN |
denotes |
at |
T1777 |
463-465 |
CD |
denotes |
48 |
T1778 |
466-467 |
NN |
denotes |
h |
T1779 |
468-482 |
RB |
denotes |
post-infection |
T1780 |
482-483 |
. |
denotes |
. |
T1781 |
483-562 |
sentence |
denotes |
The recombinant Mcm4/6/7 complexes were purified as previously described (12). |
T1782 |
484-487 |
DT |
denotes |
The |
T1783 |
509-518 |
NNS |
denotes |
complexes |
T1784 |
488-499 |
JJ |
denotes |
recombinant |
T1785 |
500-504 |
NN |
denotes |
Mcm4 |
T1786 |
504-505 |
HYPH |
denotes |
/ |
T1787 |
505-506 |
CD |
denotes |
6 |
T1788 |
506-507 |
HYPH |
denotes |
/ |
T1789 |
507-508 |
CD |
denotes |
7 |
T1790 |
524-532 |
VBN |
denotes |
purified |
T1791 |
519-523 |
VBD |
denotes |
were |
T1792 |
533-535 |
IN |
denotes |
as |
T1793 |
547-556 |
VBN |
denotes |
described |
T1794 |
536-546 |
RB |
denotes |
previously |
T1795 |
557-558 |
-LRB- |
denotes |
( |
T1796 |
558-560 |
CD |
denotes |
12 |
T1797 |
560-561 |
-RRB- |
denotes |
) |
T1798 |
561-562 |
. |
denotes |
. |
R1005 |
T1679 |
T1680 |
det |
The,baculoviruses |
R1006 |
T1680 |
T1682 |
nsubjpass |
baculoviruses,described |
R1007 |
T1681 |
T1680 |
amod |
recombinant,baculoviruses |
R1008 |
T1683 |
T1680 |
acl |
expressing,baculoviruses |
R1009 |
T1684 |
T1685 |
nmod |
His6,Mcm6 |
R1010 |
T1685 |
T1689 |
nmod |
Mcm6,proteins |
R1011 |
T1686 |
T1685 |
punct |
-,Mcm6 |
R1012 |
T1687 |
T1685 |
nmod |
Mcm4,Mcm6 |
R1013 |
T1688 |
T1685 |
punct |
/,Mcm6 |
R1014 |
T1689 |
T1683 |
dobj |
proteins,expressing |
R1015 |
T1690 |
T1685 |
cc |
or,Mcm6 |
R1016 |
T1691 |
T1692 |
compound |
Mcm7,FLAG |
R1017 |
T1692 |
T1685 |
conj |
FLAG,Mcm6 |
R1018 |
T1693 |
T1692 |
punct |
-,FLAG |
R1019 |
T1694 |
T1682 |
auxpass |
were,described |
R1020 |
T1695 |
T1682 |
advmod |
previously,described |
R1021 |
T1696 |
T1697 |
punct |
(,11 |
R1022 |
T1697 |
T1682 |
parataxis |
11,described |
R1023 |
T1698 |
T1697 |
punct |
),11 |
R1024 |
T1699 |
T1682 |
punct |
.,described |
R1025 |
T1701 |
T1702 |
nmod |
Sf9,cells |
R1026 |
T1702 |
T1707 |
nsubjpass |
cells,cultured |
R1027 |
T1703 |
T1701 |
cc |
and,Sf9 |
R1028 |
T1704 |
T1705 |
amod |
High,five |
R1029 |
T1705 |
T1701 |
conj |
five,Sf9 |
R1030 |
T1706 |
T1702 |
compound |
insect,cells |
R1031 |
T1708 |
T1707 |
auxpass |
were,cultured |
R1032 |
T1709 |
T1707 |
prep |
at,cultured |
R1033 |
T1710 |
T1711 |
nummod |
27,°C |
R1034 |
T1711 |
T1709 |
pobj |
°C,at |
R1035 |
T1712 |
T1707 |
prep |
in,cultured |
R1036 |
T1713 |
T1714 |
nmod |
Sf,SFM |
R1037 |
T1714 |
T1712 |
pobj |
SFM,in |
R1038 |
T1715 |
T1713 |
punct |
-,Sf |
R1039 |
T1716 |
T1713 |
nummod |
900,Sf |
R1040 |
T1717 |
T1714 |
nummod |
II,SFM |
R1041 |
T1718 |
T1719 |
punct |
(,Inc. |
R1042 |
T1719 |
T1714 |
parataxis |
Inc.,SFM |
R1043 |
T1720 |
T1719 |
nmod |
Life,Inc. |
R1044 |
T1721 |
T1719 |
nmod |
Technologies,Inc. |
R1045 |
T1722 |
T1719 |
punct |
", ",Inc. |
R1046 |
T1723 |
T1719 |
punct |
),Inc. |
R1047 |
T1724 |
T1714 |
cc |
and,SFM |
R1048 |
T1725 |
T1726 |
nmod |
EX,CELL |
R1049 |
T1726 |
T1728 |
nmod |
CELL,medium |
R1050 |
T1727 |
T1726 |
punct |
-,CELL |
R1051 |
T1728 |
T1714 |
conj |
medium,SFM |
R1052 |
T1729 |
T1726 |
nummod |
405,CELL |
R1053 |
T1730 |
T1731 |
punct |
(,Biosciences |
R1054 |
T1731 |
T1728 |
parataxis |
Biosciences,medium |
R1055 |
T1732 |
T1731 |
compound |
JRH,Biosciences |
R1056 |
T1733 |
T1731 |
punct |
),Biosciences |
R1057 |
T1734 |
T1707 |
punct |
", ",cultured |
R1058 |
T1735 |
T1707 |
advmod |
respectively,cultured |
R1059 |
T1736 |
T1707 |
punct |
.,cultured |
R1060 |
T1738 |
T1739 |
prep |
For,coinfected |
R1061 |
T1740 |
T1738 |
pobj |
expression,For |
R1062 |
T1741 |
T1740 |
prep |
of,expression |
R1063 |
T1742 |
T1743 |
det |
the,proteins |
R1065 |
T1744 |
T1743 |
nmod |
Mcm4,proteins |
R1066 |
T1745 |
T1744 |
punct |
/,Mcm4 |
R1067 |
T1746 |
T1744 |
nummod |
6,Mcm4 |
R1068 |
T1747 |
T1744 |
punct |
/,Mcm4 |
R1069 |
T1748 |
T1744 |
nummod |
7,Mcm4 |
R1070 |
T1749 |
T1739 |
punct |
", ",coinfected |
R1071 |
T1750 |
T1751 |
amod |
High,five |
R1072 |
T1751 |
T1752 |
nummod |
five,cells |
R1073 |
T1752 |
T1739 |
nsubjpass |
cells,coinfected |
R1074 |
T1753 |
T1739 |
auxpass |
were,coinfected |
R1075 |
T1754 |
T1739 |
prep |
with,coinfected |
R1076 |
T1755 |
T1756 |
amod |
recombinant,baculoviruses |
R1077 |
T1756 |
T1754 |
pobj |
baculoviruses,with |
R1078 |
T1757 |
T1756 |
acl |
expressing,baculoviruses |
R1079 |
T1758 |
T1759 |
det |
the,proteins |
R1081 |
T1760 |
T1761 |
compound |
His6,Mcm6 |
R1082 |
T1761 |
T1759 |
compound |
Mcm6,proteins |
R1083 |
T1762 |
T1761 |
punct |
-,Mcm6 |
R1084 |
T1763 |
T1761 |
compound |
Mcm4,Mcm6 |
R1085 |
T1764 |
T1761 |
punct |
/,Mcm6 |
R1086 |
T1765 |
T1756 |
cc |
and,baculoviruses |
R1087 |
T1766 |
T1756 |
conj |
those,baculoviruses |
R1088 |
T1767 |
T1766 |
acl |
expressing,those |
R1089 |
T1768 |
T1769 |
det |
the,FLAG |
R1090 |
T1769 |
T1767 |
dobj |
FLAG,expressing |
R1091 |
T1770 |
T1769 |
compound |
Mcm7,FLAG |
R1092 |
T1771 |
T1769 |
punct |
-,FLAG |
R1093 |
T1772 |
T1739 |
punct |
", ",coinfected |
R1094 |
T1773 |
T1739 |
cc |
and,coinfected |
R1095 |
T1774 |
T1775 |
auxpass |
were,collected |
R1096 |
T1775 |
T1739 |
conj |
collected,coinfected |
R1097 |
T1776 |
T1775 |
prep |
at,collected |
R1098 |
T1777 |
T1778 |
nummod |
48,h |
R1099 |
T1778 |
T1776 |
pobj |
h,at |
R1100 |
T1779 |
T1778 |
advmod |
post-infection,h |
R1101 |
T1780 |
T1739 |
punct |
.,coinfected |
R1102 |
T1782 |
T1783 |
det |
The,complexes |
R1103 |
T1783 |
T1790 |
nsubjpass |
complexes,purified |
R1104 |
T1784 |
T1783 |
amod |
recombinant,complexes |
R1105 |
T1785 |
T1783 |
nmod |
Mcm4,complexes |
R1106 |
T1786 |
T1785 |
punct |
/,Mcm4 |
R1107 |
T1787 |
T1785 |
nummod |
6,Mcm4 |
R1108 |
T1788 |
T1785 |
punct |
/,Mcm4 |
R1109 |
T1789 |
T1785 |
nummod |
7,Mcm4 |
R1110 |
T1791 |
T1790 |
auxpass |
were,purified |
R1111 |
T1792 |
T1793 |
mark |
as,described |
R1112 |
T1793 |
T1790 |
advcl |
described,purified |
R1113 |
T1794 |
T1793 |
advmod |
previously,described |
R1114 |
T1795 |
T1796 |
punct |
(,12 |
R1115 |
T1796 |
T1793 |
parataxis |
12,described |
R1116 |
T1797 |
T1796 |
punct |
),12 |
R1117 |
T1798 |
T1790 |
punct |
.,purified |
R1064 |
T1743 |
T1741 |
pobj |
proteins,of |
R1080 |
T1759 |
T1757 |
dobj |
proteins,expressing |