PMC:1140370 / 5357-5919
Annnotations
craft-sa-dev
The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11). Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. The recombinant Mcm4/6/7 complexes were purified as previously described (12).
craft-ca-core-ex-dev
The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11). Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. The recombinant Mcm4/6/7 complexes were purified as previously described (12).
craft-ca-core-dev
The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11). Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. The recombinant Mcm4/6/7 complexes were purified as previously described (12).
2_test
The recombinant baculoviruses expressing His6-Mcm4/Mcm6 or Mcm7-FLAG proteins were previously described (11). Sf9 and High five insect cells were cultured at 27°C in Sf-900 II SFM (Life Technologies, Inc.) and EX-CELL 405 medium (JRH Biosciences), respectively. For expression of the Mcm4/6/7 proteins, High five cells were coinfected with recombinant baculoviruses expressing the His6-Mcm4/Mcm6 proteins and those expressing the Mcm7-FLAG, and were collected at 48 h post-infection. The recombinant Mcm4/6/7 complexes were purified as previously described (12).