Id |
Subject |
Object |
Predicate |
Lexical cue |
T7743 |
0-3 |
PRP$ |
denotes |
Our |
T7744 |
4-11 |
NNS |
denotes |
results |
T7745 |
12-20 |
VBP |
denotes |
indicate |
T7746 |
21-25 |
IN |
denotes |
that |
T7747 |
62-70 |
VBN |
denotes |
required |
T7748 |
26-33 |
NN |
denotes |
thymine |
T7749 |
34-38 |
JJ |
denotes |
rich |
T7750 |
33-34 |
HYPH |
denotes |
- |
T7751 |
55-58 |
NN |
denotes |
DNA |
T7752 |
39-45 |
JJ |
denotes |
single |
T7753 |
46-54 |
VBN |
denotes |
stranded |
T7754 |
45-46 |
HYPH |
denotes |
- |
T7755 |
59-61 |
VBZ |
denotes |
is |
T7756 |
71-74 |
IN |
denotes |
for |
T7757 |
75-82 |
JJ |
denotes |
initial |
T7758 |
83-90 |
NN |
denotes |
loading |
T7759 |
91-94 |
CC |
denotes |
and |
T7760 |
95-105 |
NN |
denotes |
activation |
T7761 |
106-108 |
IN |
denotes |
of |
T7762 |
109-112 |
DT |
denotes |
the |
T7763 |
117-125 |
NN |
denotes |
helicase |
T7764 |
113-116 |
NN |
denotes |
Mcm |
T7765 |
125-126 |
. |
denotes |
. |
T7766 |
126-230 |
sentence |
denotes |
However, it is not known whether thymine sequences are required for processive unwinding of duplex DNA. |
T7767 |
127-134 |
RB |
denotes |
However |
T7768 |
146-151 |
VBN |
denotes |
known |
T7769 |
134-136 |
, |
denotes |
, |
T7770 |
136-138 |
PRP |
denotes |
it |
T7771 |
139-141 |
VBZ |
denotes |
is |
T7772 |
142-145 |
RB |
denotes |
not |
T7773 |
152-159 |
IN |
denotes |
whether |
T7774 |
182-190 |
VBN |
denotes |
required |
T7775 |
160-167 |
NN |
denotes |
thymine |
T7776 |
168-177 |
NNS |
denotes |
sequences |
T7777 |
178-181 |
VBP |
denotes |
are |
T7778 |
191-194 |
IN |
denotes |
for |
T7779 |
195-205 |
JJ |
denotes |
processive |
T7780 |
206-215 |
NN |
denotes |
unwinding |
T7781 |
216-218 |
IN |
denotes |
of |
T7782 |
219-225 |
NN |
denotes |
duplex |
T7783 |
226-229 |
NN |
denotes |
DNA |
T7784 |
229-230 |
. |
denotes |
. |
T7785 |
230-346 |
sentence |
denotes |
Therefore, we have examined whether the nucleotide composition of the duplex region affects its unwinding activity. |
T7786 |
231-240 |
RB |
denotes |
Therefore |
T7787 |
250-258 |
VBN |
denotes |
examined |
T7788 |
240-242 |
, |
denotes |
, |
T7789 |
242-244 |
PRP |
denotes |
we |
T7790 |
245-249 |
VBP |
denotes |
have |
T7791 |
259-266 |
IN |
denotes |
whether |
T7792 |
315-322 |
VBZ |
denotes |
affects |
T7793 |
267-270 |
DT |
denotes |
the |
T7794 |
282-293 |
NN |
denotes |
composition |
T7795 |
271-281 |
NN |
denotes |
nucleotide |
T7796 |
294-296 |
IN |
denotes |
of |
T7797 |
297-300 |
DT |
denotes |
the |
T7798 |
308-314 |
NN |
denotes |
region |
T7799 |
301-307 |
NN |
denotes |
duplex |
T7800 |
323-326 |
PRP$ |
denotes |
its |
T7801 |
337-345 |
NN |
denotes |
activity |
T7802 |
327-336 |
NN |
denotes |
unwinding |
T7803 |
345-346 |
. |
denotes |
. |
T7804 |
346-484 |
sentence |
denotes |
To address this issue, we constructed a series of T-tailed Y-fork structures (T-fork) containing various sequences in the duplex segment. |
T7805 |
347-349 |
TO |
denotes |
To |
T7806 |
350-357 |
VB |
denotes |
address |
T7807 |
373-384 |
VBD |
denotes |
constructed |
T7808 |
358-362 |
DT |
denotes |
this |
T7809 |
363-368 |
NN |
denotes |
issue |
T7810 |
368-370 |
, |
denotes |
, |
T7811 |
370-372 |
PRP |
denotes |
we |
T7812 |
385-386 |
DT |
denotes |
a |
T7813 |
387-393 |
NN |
denotes |
series |
T7814 |
394-396 |
IN |
denotes |
of |
T7815 |
397-398 |
NN |
denotes |
T |
T7816 |
399-405 |
VBN |
denotes |
tailed |
T7817 |
398-399 |
HYPH |
denotes |
- |
T7818 |
413-423 |
NNS |
denotes |
structures |
T7819 |
406-407 |
NN |
denotes |
Y |
T7820 |
408-412 |
NN |
denotes |
fork |
T7821 |
407-408 |
HYPH |
denotes |
- |
T7822 |
424-425 |
-LRB- |
denotes |
( |
T7823 |
425-426 |
NN |
denotes |
T |
T7824 |
427-431 |
NN |
denotes |
fork |
T7825 |
426-427 |
HYPH |
denotes |
- |
T7826 |
431-432 |
-RRB- |
denotes |
) |
T7827 |
433-443 |
VBG |
denotes |
containing |
T7828 |
444-451 |
JJ |
denotes |
various |
T7829 |
452-461 |
NNS |
denotes |
sequences |
T7830 |
462-464 |
IN |
denotes |
in |
T7831 |
465-468 |
DT |
denotes |
the |
T7832 |
476-483 |
NN |
denotes |
segment |
T7833 |
469-475 |
NN |
denotes |
duplex |
T7834 |
483-484 |
. |
denotes |
. |
T7835 |
484-557 |
sentence |
denotes |
They carried varied contents of cytosine residues on the 3′-tail strand. |
T7836 |
485-489 |
PRP |
denotes |
They |
T7837 |
490-497 |
VBD |
denotes |
carried |
T7838 |
498-504 |
VBN |
denotes |
varied |
T7839 |
505-513 |
NNS |
denotes |
contents |
T7840 |
514-516 |
IN |
denotes |
of |
T7841 |
517-525 |
NN |
denotes |
cytosine |
T7842 |
526-534 |
NNS |
denotes |
residues |
T7843 |
535-537 |
IN |
denotes |
on |
T7844 |
538-541 |
DT |
denotes |
the |
T7845 |
550-556 |
NN |
denotes |
strand |
T7846 |
542-543 |
CD |
denotes |
3 |
T7847 |
545-549 |
NN |
denotes |
tail |
T7848 |
543-544 |
SYM |
denotes |
′ |
T7849 |
544-545 |
HYPH |
denotes |
- |
T7850 |
556-557 |
. |
denotes |
. |
T7851 |
557-734 |
sentence |
denotes |
In gel shift assays, Mcm4/6/7 bound to these Y-fork substrates with identical affinity (Figure 8A), consistent with the notion that the Mcm4/6/7 binds to single-stranded tails. |
T7852 |
558-560 |
IN |
denotes |
In |
T7853 |
588-593 |
VBD |
denotes |
bound |
T7854 |
561-564 |
NN |
denotes |
gel |
T7855 |
565-570 |
NN |
denotes |
shift |
T7856 |
571-577 |
NNS |
denotes |
assays |
T7857 |
577-579 |
, |
denotes |
, |
T7858 |
579-583 |
NN |
denotes |
Mcm4 |
T7859 |
583-584 |
HYPH |
denotes |
/ |
T7860 |
584-585 |
CD |
denotes |
6 |
T7861 |
585-586 |
HYPH |
denotes |
/ |
T7862 |
586-587 |
CD |
denotes |
7 |
T7863 |
594-596 |
IN |
denotes |
to |
T7864 |
597-602 |
DT |
denotes |
these |
T7865 |
610-620 |
NNS |
denotes |
substrates |
T7866 |
603-604 |
NN |
denotes |
Y |
T7867 |
605-609 |
NN |
denotes |
fork |
T7868 |
604-605 |
HYPH |
denotes |
- |
T7869 |
621-625 |
IN |
denotes |
with |
T7870 |
626-635 |
JJ |
denotes |
identical |
T7871 |
636-644 |
NN |
denotes |
affinity |
T7872 |
645-646 |
-LRB- |
denotes |
( |
T7873 |
653-655 |
NN |
denotes |
8A |
T7874 |
646-652 |
NN |
denotes |
Figure |
T7875 |
655-656 |
-RRB- |
denotes |
) |
T7876 |
656-658 |
, |
denotes |
, |
T7877 |
658-668 |
JJ |
denotes |
consistent |
T7878 |
669-673 |
IN |
denotes |
with |
T7879 |
674-677 |
DT |
denotes |
the |
T7880 |
678-684 |
NN |
denotes |
notion |
T7881 |
685-689 |
IN |
denotes |
that |
T7882 |
703-708 |
VBZ |
denotes |
binds |
T7883 |
690-693 |
DT |
denotes |
the |
T7884 |
694-698 |
NN |
denotes |
Mcm4 |
T7885 |
698-699 |
HYPH |
denotes |
/ |
T7886 |
699-700 |
CD |
denotes |
6 |
T7887 |
700-701 |
HYPH |
denotes |
/ |
T7888 |
701-702 |
CD |
denotes |
7 |
T7889 |
709-711 |
IN |
denotes |
to |
T7890 |
712-718 |
JJ |
denotes |
single |
T7891 |
719-727 |
VBN |
denotes |
stranded |
T7892 |
718-719 |
HYPH |
denotes |
- |
T7893 |
728-733 |
NNS |
denotes |
tails |
T7894 |
733-734 |
. |
denotes |
. |
T7895 |
734-886 |
sentence |
denotes |
However, in DNA helicase assays, T-fork/(C:G)49 was hardly displaced by Mcm4/6/7, but was readily displaced by SV40 T-antigen DNA helicase (Figure 8B). |
T7896 |
735-742 |
RB |
denotes |
However |
T7897 |
794-803 |
VBN |
denotes |
displaced |
T7898 |
742-744 |
, |
denotes |
, |
T7899 |
744-746 |
IN |
denotes |
in |
T7900 |
747-750 |
NN |
denotes |
DNA |
T7901 |
751-759 |
NN |
denotes |
helicase |
T7902 |
760-766 |
NNS |
denotes |
assays |
T7903 |
766-768 |
, |
denotes |
, |
T7904 |
768-769 |
NN |
denotes |
T |
T7905 |
770-774 |
NN |
denotes |
fork |
T7906 |
769-770 |
HYPH |
denotes |
- |
T7907 |
774-775 |
HYPH |
denotes |
/ |
T7908 |
775-776 |
-LRB- |
denotes |
( |
T7909 |
778-779 |
NN |
denotes |
G |
T7910 |
776-777 |
NN |
denotes |
C |
T7911 |
777-778 |
: |
denotes |
: |
T7912 |
779-780 |
-RRB- |
denotes |
) |
T7913 |
780-782 |
CD |
denotes |
49 |
T7914 |
783-786 |
VBD |
denotes |
was |
T7915 |
787-793 |
RB |
denotes |
hardly |
T7916 |
804-806 |
IN |
denotes |
by |
T7917 |
807-811 |
NN |
denotes |
Mcm4 |
T7918 |
811-812 |
HYPH |
denotes |
/ |
T7919 |
812-813 |
CD |
denotes |
6 |
T7920 |
813-814 |
HYPH |
denotes |
/ |
T7921 |
814-815 |
CD |
denotes |
7 |
T7922 |
815-817 |
, |
denotes |
, |
T7923 |
817-820 |
CC |
denotes |
but |
T7924 |
821-824 |
VBD |
denotes |
was |
T7925 |
833-842 |
VBN |
denotes |
displaced |
T7926 |
825-832 |
RB |
denotes |
readily |
T7927 |
843-845 |
IN |
denotes |
by |
T7928 |
846-850 |
NN |
denotes |
SV40 |
T7929 |
865-873 |
NN |
denotes |
helicase |
T7930 |
851-852 |
NN |
denotes |
T |
T7931 |
853-860 |
NN |
denotes |
antigen |
T7932 |
852-853 |
HYPH |
denotes |
- |
T7933 |
861-864 |
NN |
denotes |
DNA |
T7934 |
874-875 |
-LRB- |
denotes |
( |
T7935 |
882-884 |
NN |
denotes |
8B |
T7936 |
875-881 |
NN |
denotes |
Figure |
T7937 |
884-885 |
-RRB- |
denotes |
) |
T7938 |
885-886 |
. |
denotes |
. |
T7939 |
886-1052 |
sentence |
denotes |
When thymine or adenine is inserted as every third nucleotide (repeats of CCT or CCA), the extent of unwinding increased (T-fork/(CCT:GGA)16 and T-fork/(CCA:GGT)16). |
T7940 |
887-891 |
WRB |
denotes |
When |
T7941 |
914-922 |
VBN |
denotes |
inserted |
T7942 |
892-899 |
NN |
denotes |
thymine |
T7943 |
900-902 |
CC |
denotes |
or |
T7944 |
903-910 |
NN |
denotes |
adenine |
T7945 |
911-913 |
VBZ |
denotes |
is |
T7946 |
998-1007 |
VBD |
denotes |
increased |
T7947 |
923-925 |
IN |
denotes |
as |
T7948 |
926-931 |
DT |
denotes |
every |
T7949 |
938-948 |
NN |
denotes |
nucleotide |
T7950 |
932-937 |
JJ |
denotes |
third |
T7951 |
949-950 |
-LRB- |
denotes |
( |
T7952 |
950-957 |
NNS |
denotes |
repeats |
T7953 |
958-960 |
IN |
denotes |
of |
T7954 |
961-964 |
NN |
denotes |
CCT |
T7955 |
965-967 |
CC |
denotes |
or |
T7956 |
968-971 |
NN |
denotes |
CCA |
T7957 |
971-972 |
-RRB- |
denotes |
) |
T7958 |
972-974 |
, |
denotes |
, |
T7959 |
974-977 |
DT |
denotes |
the |
T7960 |
978-984 |
NN |
denotes |
extent |
T7961 |
985-987 |
IN |
denotes |
of |
T7962 |
988-997 |
NN |
denotes |
unwinding |
T7963 |
1008-1009 |
-LRB- |
denotes |
( |
T7964 |
1021-1024 |
NN |
denotes |
GGA |
T7965 |
1009-1010 |
NN |
denotes |
T |
T7966 |
1011-1015 |
NN |
denotes |
fork |
T7967 |
1010-1011 |
HYPH |
denotes |
- |
T7968 |
1015-1016 |
HYPH |
denotes |
/ |
T7969 |
1016-1017 |
-LRB- |
denotes |
( |
T7970 |
1017-1020 |
NN |
denotes |
CCT |
T7971 |
1020-1021 |
: |
denotes |
: |
T7972 |
1024-1025 |
-RRB- |
denotes |
) |
T7973 |
1025-1027 |
CD |
denotes |
16 |
T7974 |
1028-1031 |
CC |
denotes |
and |
T7975 |
1032-1033 |
NN |
denotes |
T |
T7976 |
1034-1038 |
NN |
denotes |
fork |
T7977 |
1033-1034 |
HYPH |
denotes |
- |
T7978 |
1044-1047 |
NN |
denotes |
GGT |
T7979 |
1038-1039 |
HYPH |
denotes |
/ |
T7980 |
1039-1040 |
-LRB- |
denotes |
( |
T7981 |
1040-1043 |
NN |
denotes |
CCA |
T7982 |
1043-1044 |
: |
denotes |
: |
T7983 |
1047-1048 |
-RRB- |
denotes |
) |
T7984 |
1048-1050 |
CD |
denotes |
16 |
T7985 |
1050-1051 |
-RRB- |
denotes |
) |
T7986 |
1051-1052 |
. |
denotes |
. |
T7987 |
1052-1257 |
sentence |
denotes |
The efficiency of unwinding is roughly correlated with the content of GC pairs in the duplex segment [T-fork/(CCAA:GGTT)12, T-fork/(CCTT:GGAA)12, T-fork/(CAAA:GTTT)12 and T-fork/(CTTT:GAAA)12; Figure 8B]. |
T7988 |
1053-1056 |
DT |
denotes |
The |
T7989 |
1057-1067 |
NN |
denotes |
efficiency |
T7990 |
1092-1102 |
VBN |
denotes |
correlated |
T7991 |
1068-1070 |
IN |
denotes |
of |
T7992 |
1071-1080 |
NN |
denotes |
unwinding |
T7993 |
1081-1083 |
VBZ |
denotes |
is |
T7994 |
1084-1091 |
RB |
denotes |
roughly |
T7995 |
1103-1107 |
IN |
denotes |
with |
T7996 |
1108-1111 |
DT |
denotes |
the |
T7997 |
1112-1119 |
NN |
denotes |
content |
T7998 |
1120-1122 |
IN |
denotes |
of |
T7999 |
1123-1125 |
NN |
denotes |
GC |
T8000 |
1126-1131 |
NNS |
denotes |
pairs |
T8001 |
1132-1134 |
IN |
denotes |
in |
T8002 |
1135-1138 |
DT |
denotes |
the |
T8003 |
1146-1153 |
NN |
denotes |
segment |
T8004 |
1139-1145 |
NN |
denotes |
duplex |
T8005 |
1154-1155 |
-LRB- |
denotes |
[ |
T8006 |
1168-1172 |
NN |
denotes |
GGTT |
T8007 |
1155-1156 |
NN |
denotes |
T |
T8008 |
1157-1161 |
NN |
denotes |
fork |
T8009 |
1156-1157 |
HYPH |
denotes |
- |
T8010 |
1161-1162 |
HYPH |
denotes |
/ |
T8011 |
1162-1163 |
-LRB- |
denotes |
( |
T8012 |
1163-1167 |
NN |
denotes |
CCAA |
T8013 |
1167-1168 |
: |
denotes |
: |
T8014 |
1172-1173 |
-RRB- |
denotes |
) |
T8015 |
1173-1175 |
CD |
denotes |
12 |
T8016 |
1175-1177 |
, |
denotes |
, |
T8017 |
1177-1178 |
NN |
denotes |
T |
T8018 |
1179-1183 |
NN |
denotes |
fork |
T8019 |
1178-1179 |
HYPH |
denotes |
- |
T8020 |
1190-1194 |
NN |
denotes |
GGAA |
T8021 |
1183-1184 |
HYPH |
denotes |
/ |
T8022 |
1184-1185 |
-LRB- |
denotes |
( |
T8023 |
1185-1189 |
NN |
denotes |
CCTT |
T8024 |
1189-1190 |
: |
denotes |
: |
T8025 |
1194-1195 |
-RRB- |
denotes |
) |
T8026 |
1195-1197 |
CD |
denotes |
12 |
T8027 |
1197-1199 |
, |
denotes |
, |
T8028 |
1199-1200 |
NN |
denotes |
T |
T8029 |
1201-1205 |
NN |
denotes |
fork |
T8030 |
1200-1201 |
HYPH |
denotes |
- |
T8031 |
1212-1216 |
NN |
denotes |
GTTT |
T8032 |
1205-1206 |
HYPH |
denotes |
/ |
T8033 |
1206-1207 |
-LRB- |
denotes |
( |
T8034 |
1207-1211 |
NN |
denotes |
CAAA |
T8035 |
1211-1212 |
: |
denotes |
: |
T8036 |
1216-1217 |
-RRB- |
denotes |
) |
T8037 |
1217-1219 |
CD |
denotes |
12 |
T8038 |
1220-1223 |
CC |
denotes |
and |
T8039 |
1224-1225 |
NN |
denotes |
T |
T8040 |
1226-1230 |
NN |
denotes |
fork |
T8041 |
1225-1226 |
HYPH |
denotes |
- |
T8042 |
1237-1241 |
NN |
denotes |
GAAA |
T8043 |
1230-1231 |
HYPH |
denotes |
/ |
T8044 |
1231-1232 |
-LRB- |
denotes |
( |
T8045 |
1232-1236 |
NN |
denotes |
CTTT |
T8046 |
1236-1237 |
: |
denotes |
: |
T8047 |
1241-1242 |
-RRB- |
denotes |
) |
T8048 |
1242-1244 |
CD |
denotes |
12 |
T8049 |
1244-1245 |
: |
denotes |
; |
T8050 |
1253-1255 |
NN |
denotes |
8B |
T8051 |
1246-1252 |
NN |
denotes |
Figure |
T8052 |
1255-1256 |
-RRB- |
denotes |
] |
T8053 |
1256-1257 |
. |
denotes |
. |
T8054 |
1257-1353 |
sentence |
denotes |
It appears that the duplex segment containing <50% GC pairs is efficiently unwound (Figure 8C). |
T8055 |
1258-1260 |
PRP |
denotes |
It |
T8056 |
1261-1268 |
VBZ |
denotes |
appears |
T8057 |
1269-1273 |
IN |
denotes |
that |
T8058 |
1333-1340 |
VBN |
denotes |
unwound |
T8059 |
1274-1277 |
DT |
denotes |
the |
T8060 |
1285-1292 |
NN |
denotes |
segment |
T8061 |
1278-1284 |
NN |
denotes |
duplex |
T8062 |
1293-1303 |
VBG |
denotes |
containing |
T8063 |
1304-1305 |
SYM |
denotes |
< |
T8064 |
1305-1307 |
CD |
denotes |
50 |
T8065 |
1307-1308 |
NN |
denotes |
% |
T8066 |
1312-1317 |
NNS |
denotes |
pairs |
T8067 |
1309-1311 |
NN |
denotes |
GC |
T8068 |
1318-1320 |
VBZ |
denotes |
is |
T8069 |
1321-1332 |
RB |
denotes |
efficiently |
T8070 |
1341-1342 |
-LRB- |
denotes |
( |
T8071 |
1349-1351 |
NN |
denotes |
8C |
T8072 |
1342-1348 |
NN |
denotes |
Figure |
T8073 |
1351-1352 |
-RRB- |
denotes |
) |
T8074 |
1352-1353 |
. |
denotes |
. |
R5143 |
T7743 |
T7744 |
poss |
Our,results |
R5144 |
T7744 |
T7745 |
nsubj |
results,indicate |
R5145 |
T7746 |
T7747 |
mark |
that,required |
R5146 |
T7747 |
T7745 |
ccomp |
required,indicate |
R5147 |
T7748 |
T7749 |
npadvmod |
thymine,rich |
R5148 |
T7749 |
T7751 |
amod |
rich,DNA |
R5149 |
T7750 |
T7749 |
punct |
-,rich |
R5150 |
T7751 |
T7747 |
nsubjpass |
DNA,required |
R5151 |
T7752 |
T7753 |
amod |
single,stranded |
R5152 |
T7753 |
T7751 |
amod |
stranded,DNA |
R5153 |
T7754 |
T7753 |
punct |
-,stranded |
R5154 |
T7755 |
T7747 |
auxpass |
is,required |
R5155 |
T7756 |
T7747 |
prep |
for,required |
R5156 |
T7757 |
T7758 |
amod |
initial,loading |
R5157 |
T7758 |
T7756 |
pobj |
loading,for |
R5158 |
T7759 |
T7758 |
cc |
and,loading |
R5159 |
T7760 |
T7758 |
conj |
activation,loading |
R5160 |
T7761 |
T7758 |
prep |
of,loading |
R5161 |
T7762 |
T7763 |
det |
the,helicase |
R5162 |
T7763 |
T7761 |
pobj |
helicase,of |
R5163 |
T7764 |
T7763 |
compound |
Mcm,helicase |
R5164 |
T7765 |
T7745 |
punct |
.,indicate |
R5165 |
T7767 |
T7768 |
advmod |
However,known |
R5166 |
T7769 |
T7768 |
punct |
", ",known |
R5167 |
T7770 |
T7768 |
nsubjpass |
it,known |
R5168 |
T7771 |
T7768 |
auxpass |
is,known |
R5169 |
T7772 |
T7768 |
neg |
not,known |
R5170 |
T7773 |
T7774 |
mark |
whether,required |
R5171 |
T7774 |
T7768 |
ccomp |
required,known |
R5172 |
T7775 |
T7776 |
compound |
thymine,sequences |
R5173 |
T7776 |
T7774 |
nsubjpass |
sequences,required |
R5174 |
T7777 |
T7774 |
auxpass |
are,required |
R5175 |
T7778 |
T7774 |
prep |
for,required |
R5176 |
T7779 |
T7780 |
amod |
processive,unwinding |
R5177 |
T7780 |
T7778 |
pobj |
unwinding,for |
R5178 |
T7781 |
T7780 |
prep |
of,unwinding |
R5179 |
T7782 |
T7783 |
compound |
duplex,DNA |
R5180 |
T7783 |
T7781 |
pobj |
DNA,of |
R5181 |
T7784 |
T7768 |
punct |
.,known |
R5182 |
T7786 |
T7787 |
advmod |
Therefore,examined |
R5183 |
T7788 |
T7787 |
punct |
", ",examined |
R5184 |
T7789 |
T7787 |
nsubj |
we,examined |
R5185 |
T7790 |
T7787 |
aux |
have,examined |
R5186 |
T7791 |
T7792 |
mark |
whether,affects |
R5187 |
T7792 |
T7787 |
ccomp |
affects,examined |
R5188 |
T7793 |
T7794 |
det |
the,composition |
R5189 |
T7794 |
T7792 |
nsubj |
composition,affects |
R5190 |
T7795 |
T7794 |
compound |
nucleotide,composition |
R5191 |
T7796 |
T7794 |
prep |
of,composition |
R5192 |
T7797 |
T7798 |
det |
the,region |
R5193 |
T7798 |
T7796 |
pobj |
region,of |
R5194 |
T7799 |
T7798 |
compound |
duplex,region |
R5195 |
T7800 |
T7801 |
poss |
its,activity |
R5196 |
T7801 |
T7792 |
dobj |
activity,affects |
R5197 |
T7802 |
T7801 |
compound |
unwinding,activity |
R5198 |
T7803 |
T7787 |
punct |
.,examined |
R5199 |
T7805 |
T7806 |
aux |
To,address |
R5200 |
T7806 |
T7807 |
advcl |
address,constructed |
R5201 |
T7808 |
T7809 |
det |
this,issue |
R5202 |
T7809 |
T7806 |
dobj |
issue,address |
R5203 |
T7810 |
T7807 |
punct |
", ",constructed |
R5204 |
T7811 |
T7807 |
nsubj |
we,constructed |
R5205 |
T7812 |
T7813 |
det |
a,series |
R5206 |
T7813 |
T7807 |
dobj |
series,constructed |
R5207 |
T7814 |
T7813 |
prep |
of,series |
R5208 |
T7815 |
T7816 |
npadvmod |
T,tailed |
R5209 |
T7816 |
T7818 |
amod |
tailed,structures |
R5210 |
T7817 |
T7816 |
punct |
-,tailed |
R5211 |
T7818 |
T7814 |
pobj |
structures,of |
R5212 |
T7819 |
T7820 |
compound |
Y,fork |
R5213 |
T7820 |
T7818 |
compound |
fork,structures |
R5214 |
T7821 |
T7820 |
punct |
-,fork |
R5215 |
T7822 |
T7818 |
punct |
(,structures |
R5216 |
T7823 |
T7824 |
compound |
T,fork |
R5217 |
T7824 |
T7818 |
appos |
fork,structures |
R5218 |
T7825 |
T7824 |
punct |
-,fork |
R5219 |
T7826 |
T7818 |
punct |
),structures |
R5220 |
T7827 |
T7818 |
acl |
containing,structures |
R5221 |
T7828 |
T7829 |
amod |
various,sequences |
R5222 |
T7829 |
T7827 |
dobj |
sequences,containing |
R5223 |
T7830 |
T7807 |
prep |
in,constructed |
R5224 |
T7831 |
T7832 |
det |
the,segment |
R5225 |
T7832 |
T7830 |
pobj |
segment,in |
R5226 |
T7833 |
T7832 |
compound |
duplex,segment |
R5227 |
T7834 |
T7807 |
punct |
.,constructed |
R5228 |
T7836 |
T7837 |
nsubj |
They,carried |
R5229 |
T7838 |
T7839 |
amod |
varied,contents |
R5230 |
T7839 |
T7837 |
dobj |
contents,carried |
R5231 |
T7840 |
T7839 |
prep |
of,contents |
R5232 |
T7841 |
T7842 |
compound |
cytosine,residues |
R5233 |
T7842 |
T7840 |
pobj |
residues,of |
R5234 |
T7843 |
T7837 |
prep |
on,carried |
R5235 |
T7844 |
T7845 |
det |
the,strand |
R5236 |
T7845 |
T7843 |
pobj |
strand,on |
R5237 |
T7846 |
T7847 |
nummod |
3,tail |
R5238 |
T7847 |
T7845 |
compound |
tail,strand |
R5239 |
T7848 |
T7846 |
punct |
′,3 |
R5240 |
T7849 |
T7847 |
punct |
-,tail |
R5241 |
T7850 |
T7837 |
punct |
.,carried |
R5242 |
T7852 |
T7853 |
prep |
In,bound |
R5243 |
T7854 |
T7855 |
compound |
gel,shift |
R5244 |
T7855 |
T7856 |
compound |
shift,assays |
R5245 |
T7856 |
T7852 |
pobj |
assays,In |
R5246 |
T7857 |
T7853 |
punct |
", ",bound |
R5247 |
T7858 |
T7853 |
nsubj |
Mcm4,bound |
R5248 |
T7859 |
T7858 |
punct |
/,Mcm4 |
R5249 |
T7860 |
T7858 |
nummod |
6,Mcm4 |
R5250 |
T7861 |
T7858 |
punct |
/,Mcm4 |
R5251 |
T7862 |
T7858 |
nummod |
7,Mcm4 |
R5252 |
T7863 |
T7853 |
prep |
to,bound |
R5253 |
T7864 |
T7865 |
det |
these,substrates |
R5254 |
T7865 |
T7863 |
pobj |
substrates,to |
R5255 |
T7866 |
T7867 |
compound |
Y,fork |
R5256 |
T7867 |
T7865 |
compound |
fork,substrates |
R5257 |
T7868 |
T7867 |
punct |
-,fork |
R5258 |
T7869 |
T7853 |
prep |
with,bound |
R5259 |
T7870 |
T7871 |
amod |
identical,affinity |
R5260 |
T7871 |
T7869 |
pobj |
affinity,with |
R5261 |
T7872 |
T7873 |
punct |
(,8A |
R5262 |
T7873 |
T7871 |
parataxis |
8A,affinity |
R5263 |
T7874 |
T7873 |
compound |
Figure,8A |
R5264 |
T7875 |
T7873 |
punct |
),8A |
R5265 |
T7876 |
T7853 |
punct |
", ",bound |
R5266 |
T7877 |
T7853 |
advcl |
consistent,bound |
R5267 |
T7878 |
T7877 |
prep |
with,consistent |
R5268 |
T7879 |
T7880 |
det |
the,notion |
R5269 |
T7880 |
T7878 |
pobj |
notion,with |
R5270 |
T7881 |
T7882 |
mark |
that,binds |
R5271 |
T7882 |
T7880 |
acl |
binds,notion |
R5272 |
T7883 |
T7884 |
det |
the,Mcm4 |
R5273 |
T7884 |
T7882 |
nsubj |
Mcm4,binds |
R5274 |
T7885 |
T7884 |
punct |
/,Mcm4 |
R5275 |
T7886 |
T7884 |
nummod |
6,Mcm4 |
R5276 |
T7887 |
T7884 |
punct |
/,Mcm4 |
R5277 |
T7888 |
T7884 |
nummod |
7,Mcm4 |
R5278 |
T7889 |
T7882 |
prep |
to,binds |
R5279 |
T7890 |
T7891 |
amod |
single,stranded |
R5280 |
T7891 |
T7893 |
amod |
stranded,tails |
R5281 |
T7892 |
T7891 |
punct |
-,stranded |
R5282 |
T7893 |
T7889 |
pobj |
tails,to |
R5283 |
T7894 |
T7853 |
punct |
.,bound |
R5284 |
T7896 |
T7897 |
advmod |
However,displaced |
R5285 |
T7898 |
T7897 |
punct |
", ",displaced |
R5286 |
T7899 |
T7897 |
prep |
in,displaced |
R5287 |
T7900 |
T7901 |
compound |
DNA,helicase |
R5288 |
T7901 |
T7902 |
compound |
helicase,assays |
R5289 |
T7902 |
T7899 |
pobj |
assays,in |
R5290 |
T7903 |
T7897 |
punct |
", ",displaced |
R5291 |
T7904 |
T7905 |
compound |
T,fork |
R5292 |
T7905 |
T7897 |
nsubjpass |
fork,displaced |
R5293 |
T7906 |
T7905 |
punct |
-,fork |
R5294 |
T7907 |
T7905 |
punct |
/,fork |
R5295 |
T7908 |
T7909 |
punct |
(,G |
R5296 |
T7909 |
T7905 |
appos |
G,fork |
R5297 |
T7910 |
T7909 |
nmod |
C,G |
R5298 |
T7911 |
T7909 |
punct |
:,G |
R5299 |
T7912 |
T7909 |
punct |
),G |
R5300 |
T7913 |
T7909 |
nummod |
49,G |
R5301 |
T7914 |
T7897 |
auxpass |
was,displaced |
R5302 |
T7915 |
T7897 |
advmod |
hardly,displaced |
R5303 |
T7916 |
T7897 |
agent |
by,displaced |
R5304 |
T7917 |
T7916 |
pobj |
Mcm4,by |
R5305 |
T7918 |
T7917 |
punct |
/,Mcm4 |
R5306 |
T7919 |
T7917 |
nummod |
6,Mcm4 |
R5307 |
T7920 |
T7917 |
punct |
/,Mcm4 |
R5308 |
T7921 |
T7917 |
nummod |
7,Mcm4 |
R5309 |
T7922 |
T7897 |
punct |
", ",displaced |
R5310 |
T7923 |
T7897 |
cc |
but,displaced |
R5311 |
T7924 |
T7925 |
auxpass |
was,displaced |
R5312 |
T7925 |
T7897 |
conj |
displaced,displaced |
R5313 |
T7926 |
T7925 |
advmod |
readily,displaced |
R5314 |
T7927 |
T7925 |
agent |
by,displaced |
R5315 |
T7928 |
T7929 |
compound |
SV40,helicase |
R5316 |
T7929 |
T7927 |
pobj |
helicase,by |
R5317 |
T7930 |
T7931 |
compound |
T,antigen |
R5318 |
T7931 |
T7929 |
compound |
antigen,helicase |
R5319 |
T7932 |
T7931 |
punct |
-,antigen |
R5320 |
T7933 |
T7929 |
compound |
DNA,helicase |
R5321 |
T7934 |
T7935 |
punct |
(,8B |
R5322 |
T7935 |
T7925 |
parataxis |
8B,displaced |
R5323 |
T7936 |
T7935 |
compound |
Figure,8B |
R5324 |
T7937 |
T7935 |
punct |
),8B |
R5325 |
T7938 |
T7897 |
punct |
.,displaced |
R5326 |
T7940 |
T7941 |
advmod |
When,inserted |
R5327 |
T7941 |
T7946 |
advcl |
inserted,increased |
R5328 |
T7942 |
T7941 |
nsubjpass |
thymine,inserted |
R5329 |
T7943 |
T7942 |
cc |
or,thymine |
R5330 |
T7944 |
T7942 |
conj |
adenine,thymine |
R5331 |
T7945 |
T7941 |
auxpass |
is,inserted |
R5332 |
T7947 |
T7941 |
prep |
as,inserted |
R5333 |
T7948 |
T7949 |
det |
every,nucleotide |
R5334 |
T7949 |
T7947 |
pobj |
nucleotide,as |
R5335 |
T7950 |
T7949 |
amod |
third,nucleotide |
R5336 |
T7951 |
T7952 |
punct |
(,repeats |
R5337 |
T7952 |
T7949 |
parataxis |
repeats,nucleotide |
R5338 |
T7953 |
T7952 |
prep |
of,repeats |
R5339 |
T7954 |
T7953 |
pobj |
CCT,of |
R5340 |
T7955 |
T7954 |
cc |
or,CCT |
R5341 |
T7956 |
T7954 |
conj |
CCA,CCT |
R5342 |
T7957 |
T7952 |
punct |
),repeats |
R5343 |
T7958 |
T7946 |
punct |
", ",increased |
R5344 |
T7959 |
T7960 |
det |
the,extent |
R5345 |
T7960 |
T7946 |
nsubj |
extent,increased |
R5346 |
T7961 |
T7960 |
prep |
of,extent |
R5347 |
T7962 |
T7961 |
pobj |
unwinding,of |
R5348 |
T7963 |
T7964 |
punct |
(,GGA |
R5349 |
T7964 |
T7946 |
dobj |
GGA,increased |
R5350 |
T7965 |
T7966 |
nmod |
T,fork |
R5351 |
T7966 |
T7964 |
nmod |
fork,GGA |
R5352 |
T7967 |
T7966 |
punct |
-,fork |
R5353 |
T7968 |
T7964 |
punct |
/,GGA |
R5354 |
T7969 |
T7964 |
punct |
(,GGA |
R5355 |
T7970 |
T7964 |
nmod |
CCT,GGA |
R5356 |
T7971 |
T7964 |
punct |
:,GGA |
R5357 |
T7972 |
T7964 |
punct |
),GGA |
R5358 |
T7973 |
T7964 |
nummod |
16,GGA |
R5359 |
T7974 |
T7964 |
cc |
and,GGA |
R5360 |
T7975 |
T7976 |
nmod |
T,fork |
R5361 |
T7976 |
T7978 |
nmod |
fork,GGT |
R5362 |
T7977 |
T7976 |
punct |
-,fork |
R5363 |
T7978 |
T7964 |
conj |
GGT,GGA |
R5364 |
T7979 |
T7978 |
punct |
/,GGT |
R5365 |
T7980 |
T7978 |
punct |
(,GGT |
R5366 |
T7981 |
T7978 |
nmod |
CCA,GGT |
R5367 |
T7982 |
T7978 |
punct |
:,GGT |
R5368 |
T7983 |
T7978 |
punct |
),GGT |
R5369 |
T7984 |
T7978 |
nummod |
16,GGT |
R5370 |
T7985 |
T7946 |
punct |
),increased |
R5371 |
T7986 |
T7946 |
punct |
.,increased |
R5372 |
T7988 |
T7989 |
det |
The,efficiency |
R5373 |
T7989 |
T7990 |
nsubjpass |
efficiency,correlated |
R5374 |
T7991 |
T7989 |
prep |
of,efficiency |
R5375 |
T7992 |
T7991 |
pobj |
unwinding,of |
R5376 |
T7993 |
T7990 |
auxpass |
is,correlated |
R5377 |
T7994 |
T7990 |
advmod |
roughly,correlated |
R5378 |
T7995 |
T7990 |
prep |
with,correlated |
R5379 |
T7996 |
T7997 |
det |
the,content |
R5380 |
T7997 |
T7995 |
pobj |
content,with |
R5381 |
T7998 |
T7997 |
prep |
of,content |
R5382 |
T7999 |
T8000 |
compound |
GC,pairs |
R5383 |
T8000 |
T7998 |
pobj |
pairs,of |
R5384 |
T8001 |
T8000 |
prep |
in,pairs |
R5385 |
T8002 |
T8003 |
det |
the,segment |
R5386 |
T8003 |
T8001 |
pobj |
segment,in |
R5387 |
T8004 |
T8003 |
compound |
duplex,segment |
R5388 |
T8005 |
T8006 |
punct |
[,GGTT |
R5389 |
T8006 |
T8000 |
appos |
GGTT,pairs |
R5390 |
T8007 |
T8008 |
nmod |
T,fork |
R5391 |
T8008 |
T8006 |
nmod |
fork,GGTT |
R5392 |
T8009 |
T8008 |
punct |
-,fork |
R5393 |
T8010 |
T8006 |
punct |
/,GGTT |
R5394 |
T8011 |
T8006 |
punct |
(,GGTT |
R5395 |
T8012 |
T8006 |
nmod |
CCAA,GGTT |
R5396 |
T8013 |
T8006 |
punct |
:,GGTT |
R5397 |
T8014 |
T8006 |
punct |
),GGTT |
R5398 |
T8015 |
T8006 |
nummod |
12,GGTT |
R5399 |
T8016 |
T8006 |
punct |
", ",GGTT |
R5400 |
T8017 |
T8018 |
nmod |
T,fork |
R5401 |
T8018 |
T8020 |
nmod |
fork,GGAA |
R5402 |
T8019 |
T8018 |
punct |
-,fork |
R5403 |
T8020 |
T8006 |
conj |
GGAA,GGTT |
R5404 |
T8021 |
T8020 |
punct |
/,GGAA |
R5405 |
T8022 |
T8020 |
punct |
(,GGAA |
R5406 |
T8023 |
T8020 |
nmod |
CCTT,GGAA |
R5407 |
T8024 |
T8020 |
punct |
:,GGAA |
R5408 |
T8025 |
T8020 |
punct |
),GGAA |
R5409 |
T8026 |
T8020 |
nummod |
12,GGAA |
R5410 |
T8027 |
T8020 |
punct |
", ",GGAA |
R5411 |
T8028 |
T8029 |
nmod |
T,fork |
R5412 |
T8029 |
T8031 |
nmod |
fork,GTTT |
R5413 |
T8030 |
T8029 |
punct |
-,fork |
R5414 |
T8031 |
T8020 |
conj |
GTTT,GGAA |
R5415 |
T8032 |
T8031 |
punct |
/,GTTT |
R5416 |
T8033 |
T8031 |
punct |
(,GTTT |
R5417 |
T8034 |
T8031 |
nmod |
CAAA,GTTT |
R5418 |
T8035 |
T8031 |
punct |
:,GTTT |
R5419 |
T8036 |
T8031 |
punct |
),GTTT |
R5420 |
T8037 |
T8031 |
nummod |
12,GTTT |
R5421 |
T8038 |
T8031 |
cc |
and,GTTT |
R5422 |
T8039 |
T8040 |
nmod |
T,fork |
R5423 |
T8040 |
T8042 |
nmod |
fork,GAAA |
R5424 |
T8041 |
T8040 |
punct |
-,fork |
R5425 |
T8042 |
T8031 |
conj |
GAAA,GTTT |
R5426 |
T8043 |
T8042 |
punct |
/,GAAA |
R5427 |
T8044 |
T8042 |
punct |
(,GAAA |
R5428 |
T8045 |
T8042 |
nmod |
CTTT,GAAA |
R5429 |
T8046 |
T8042 |
punct |
:,GAAA |
R5430 |
T8047 |
T8042 |
punct |
),GAAA |
R5431 |
T8048 |
T8042 |
nummod |
12,GAAA |
R5432 |
T8049 |
T8050 |
punct |
;,8B |
R5433 |
T8050 |
T7990 |
parataxis |
8B,correlated |
R5434 |
T8051 |
T8050 |
compound |
Figure,8B |
R5435 |
T8052 |
T8050 |
punct |
],8B |
R5436 |
T8053 |
T7990 |
punct |
.,correlated |
R5437 |
T8055 |
T8056 |
nsubj |
It,appears |
R5438 |
T8057 |
T8058 |
mark |
that,unwound |
R5439 |
T8058 |
T8056 |
ccomp |
unwound,appears |
R5440 |
T8059 |
T8060 |
det |
the,segment |
R5441 |
T8060 |
T8058 |
nsubjpass |
segment,unwound |
R5442 |
T8061 |
T8060 |
compound |
duplex,segment |
R5443 |
T8062 |
T8060 |
acl |
containing,segment |
R5444 |
T8063 |
T8064 |
punct |
<,50 |
R5445 |
T8064 |
T8065 |
nummod |
50,% |
R5446 |
T8065 |
T8066 |
compound |
%,pairs |
R5447 |
T8066 |
T8062 |
dobj |
pairs,containing |
R5448 |
T8067 |
T8066 |
compound |
GC,pairs |
R5449 |
T8068 |
T8058 |
auxpass |
is,unwound |
R5450 |
T8069 |
T8058 |
advmod |
efficiently,unwound |
R5451 |
T8070 |
T8071 |
punct |
(,8C |
R5452 |
T8071 |
T8058 |
parataxis |
8C,unwound |
R5453 |
T8072 |
T8071 |
compound |
Figure,8C |
R5454 |
T8073 |
T8071 |
punct |
),8C |
R5455 |
T8074 |
T8056 |
punct |
.,appears |