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PubMed:9988767 JSONTXT 35 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-73 Sentence denotes Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
T2 74-177 Sentence denotes Isolation, characterization, and expression of human cdnas and identification of distinct genomic loci.
T3 178-322 Sentence denotes 3-O-Sulfated glucosaminyl residues are rare constituents of heparan sulfate and are essential for the activity of anticoagulant heparan sulfate.
T4 323-497 Sentence denotes Cellular production of the critical active structure is controlled by the rate-limiting enzyme, heparan sulfate D-glucosaminyl 3-O-sulfotransferase-1 (3-OST-1) (EC 2.8.2.23).
T5 498-658 Sentence denotes We have probed the expressed sequence tag data base with the carboxyl-terminal sulfotransferase domain of 3-OST-1 to reveal three novel, incomplete human cDNAs.
T6 659-727 Sentence denotes These were utilized in library screens to isolate full-length cDNAs.
T7 728-883 Sentence denotes Clones corresponding to predominant transcripts were obtained for the 367-, 406-, and 390-amino acid enzymes 3-OST-2, 3-OST-3A, and 3-OST-3B, respectively.
T8 884-1070 Sentence denotes These type II integral membrane proteins are comprised of a divergent amino-terminal region and a very homologous carboxyl-terminal sulfotransferase domain of approximately 260 residues.
T9 1071-1125 Sentence denotes Also recovered were partial length clones for 3-OST-4.
T10 1126-1269 Sentence denotes Expression of the full-length enzymes confirms the 3-O-sulfation of specific glucosaminyl residues within heparan sulfate (Liu, J., Shworak, N.
T11 1270-1297 Sentence denotes W., Sinaÿ, P., Schwartz, J.
T12 1298-1300 Sentence denotes J.
T13 1301-1322 Sentence denotes Zhang, L., Fritze, L.
T14 1323-1325 Sentence denotes M.
T15 1326-1347 Sentence denotes S., and Rosenberg, R.
T16 1348-1360 Sentence denotes D. (1999) J.
T17 1361-1366 Sentence denotes Biol.
T18 1367-1372 Sentence denotes Chem.
T19 1373-1389 Sentence denotes 274, 5185-5192).
T20 1390-1510 Sentence denotes Southern analyses suggest the human 3OST1, 3OST2, and 3OST4 genes, and the corresponding mouse isologs, are single copy.
T21 1511-1614 Sentence denotes However, 3OST3A and 3OST3B genes are each duplicated in humans and show at least one copy each in mice.
T22 1615-1762 Sentence denotes Intriguingly, the entire sulfotransferase domain sequence of the 3-OST-3B cDNA (774 base pairs) was 99.2% identical to the same region of 3-OST-3A.
T23 1763-1920 Sentence denotes Together, these data argue that the structure of this functionally important region is actively maintained by gene conversion between 3OST3A and 3OST3B loci.
T24 1921-2134 Sentence denotes Interspecific mouse back-cross analysis identified the loci for mouse 3Ost genes and syntenic assignments of corresponding human isologs were confirmed by the identification of mapped sequence-tagged site markers.
T25 2135-2357 Sentence denotes Northern blot analyses indicate brain exclusive and brain predominant expression of 3-OST-4 and 3-OST-2 transcripts, respectively; whereas, 3-OST-3A and 3-OST-3B isoforms show widespread expression of multiple transcripts.
T26 2358-2492 Sentence denotes The reiteration and conservation of the 3-OST sulfotransferase domain suggest that this structure is a self-contained functional unit.
T27 2493-2720 Sentence denotes Moreover, the extensive number of 3OST genes with diverse expression patterns of multiple transcripts suggests that the novel 3-OST enzymes, like 3-OST-1, regulate important biologic properties of heparan sulfate proteoglycans.