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PubMed:9915862 JSONTXT 39 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-128 Sentence denotes Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches.
T2 129-200 Sentence denotes Mucin-type O-glycans are classified according to their core structures.
T3 201-362 Sentence denotes Among them, cores 2 and 4 are important for having N-acetyllactosamine side chains, which can be further modified to express various functional oligosaccharides.
T4 363-625 Sentence denotes Previously, we discovered by cloning cDNAs that the core 2 branching enzyme, termed core 2 beta-1,6-N-acetylglucosaminyltransferase-leukocyte type (C2GnT-L), is highly homologous to the I branching beta-1, 6-N-acetylglucosaminyltransferase (IGnT) (Bierhuizen, M.
T5 626-628 Sentence denotes F.
T6 629-680 Sentence denotes A., Mattei, M.-G., and Fukuda, M. (1993) Genes Dev.
T7 681-693 Sentence denotes 7, 468-478).
T8 694-839 Sentence denotes Using these homologous sequences as probes, we identified an expressed sequence tag in dbEST, which has significant homology to C2GnT-L and IGnT.
T9 840-1020 Sentence denotes This approach, together with 5'and 3' rapid amplification of cDNA ends, yielded a human cDNA that encompasses a whole coding region of an enzyme, termed C2GnT-mucin type (C2GnT-M).
T10 1021-1104 Sentence denotes C2GnT-M has 48.2 and 33.8% identity with C2GnT-L and IGnT at the amino acid levels.
T11 1105-1230 Sentence denotes The expression of C2GnT-M cDNA directed the expression of core 2 branched oligosaccharides and I antigen on the cell surface.
T12 1231-1347 Sentence denotes Moreover, a soluble chimeric C2GnT-M had core 4 branching activity in addition to core 2 and I branching activities.
T13 1348-1443 Sentence denotes A soluble chimeric C2GnT-L, in contrast, almost exclusively contains core 2 branching activity.
T14 1444-1604 Sentence denotes Northern blot analysis demonstrated that the C2GnT-M transcripts are heavily expressed in colon, small intestine, trachea, and stomach, where mucin is produced.
T15 1605-1713 Sentence denotes In contrast, the transcripts of C2GnT-L were more widely detected, including the lymph node and bone marrow.
T16 1714-1921 Sentence denotes These results indicate that the newly cloned C2GnT-M plays a critical role in O-glycan synthesis in mucins and might have distinctly different roles in oligosaccharide ligand formation compared with C2GnT-L.